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Protein

Phosphoethanolamine/phosphocholine phosphatase

Gene

Phospho1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.1 Publication

Catalytic activityi

O-phosphoethanolamine + H2O = ethanolamine + phosphate.
Phosphocholine + H2O = choline + phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei32 – 321NucleophileBy similarity
Metal bindingi32 – 321MagnesiumBy similarity
Active sitei34 – 341Proton donorBy similarity
Metal bindingi34 – 341MagnesiumBy similarity
Binding sitei43 – 431SubstrateBy similarity
Binding sitei123 – 1231SubstrateBy similarity
Metal bindingi203 – 2031MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

  • bone mineralization involved in bone maturation Source: MGI
  • dephosphorylation Source: MGI
  • endochondral ossification Source: MGI
  • regulation of bone mineralization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Mineral balance

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.3.75. 3474.
ReactomeiR-MMU-1483191. Synthesis of PC.
R-MMU-1483213. Synthesis of PE.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoethanolamine/phosphocholine phosphatase (EC:3.1.3.75)
Gene namesi
Name:Phospho1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2447348. Phospho1.

Subcellular locationi

GO - Cellular componenti

  • extracellular membrane-bounded organelle Source: MGI
  • proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Phosphoethanolamine/phosphocholine phosphatasePRO_0000068830Add
BLAST

Proteomic databases

MaxQBiQ8R2H9.
PaxDbiQ8R2H9.
PRIDEiQ8R2H9.

PTM databases

PhosphoSiteiQ8R2H9.

Expressioni

Tissue specificityi

Has a 120-fold higher level of expression in bone compared with a range of soft tissues.1 Publication

Gene expression databases

BgeeiQ8R2H9.
ExpressionAtlasiQ8R2H9. baseline and differential.
GenevisibleiQ8R2H9. MM.

Interactioni

Protein-protein interaction databases

BioGridi231927. 7 interactions.
IntActiQ8R2H9. 7 interactions.
STRINGi10090.ENSMUSP00000057858.

Structurei

3D structure databases

ProteinModelPortaliQ8R2H9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3120. Eukaryota.
ENOG4111NAQ. LUCA.
GeneTreeiENSGT00390000007741.
HOGENOMiHOG000231038.
HOVERGENiHBG080058.
InParanoidiQ8R2H9.
KOiK06124.
OMAiSPGMGDL.
PhylomeDBiQ8R2H9.
TreeFamiTF300112.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006383. HAD-SF_hydro_IB_PSP-like.
IPR016965. Pase_PHOSPHO-typ.
IPR006384. PyrdxlP_Pase-rel.
[Graphical view]
PfamiPF06888. Put_Phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01489. DKMTPPase-SF. 1 hit.
TIGR01488. HAD-SF-IB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8R2H9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGCFPAVGL RCLSRDGRMA APGAPRFLLT FDFDETIVDE NSDDSIVRAA
60 70 80 90 100
PGQQLPESLR ATYREGYYNE YMQRVFKYLG EQGVRPRDLR AVYETIPLSP
110 120 130 140 150
GMGDLLQFIA KQGSCFEVIL ISDANTFGVE SALRAAGHHS LFRRILSNPS
160 170 180 190 200
GPDARGLLTL RPFHTHSCSR CPANMCKHKV LSEYLRERAR DGVHFERLFY
210 220 230 240 250
VGDGANDFCP MGLLAGGDVA FPRRGYPMHR LIQEAQKAEP SSFRAHVVPW
260
ETAADVRQHL QQVLKMC
Length:267
Mass (Da):29,911
Last modified:June 1, 2002 - v1
Checksum:i0CEEE66019C0CEA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ457190 mRNA. Translation: CAD29804.1.
AL593858 Genomic DNA. Translation: CAM15510.1.
CCDSiCCDS36288.1.
RefSeqiNP_694744.1. NM_153104.3.
UniGeneiMm.133075.

Genome annotation databases

EnsembliENSMUST00000054173; ENSMUSP00000057858; ENSMUSG00000050860.
GeneIDi237928.
KEGGimmu:237928.
UCSCiuc007las.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ457190 mRNA. Translation: CAD29804.1.
AL593858 Genomic DNA. Translation: CAM15510.1.
CCDSiCCDS36288.1.
RefSeqiNP_694744.1. NM_153104.3.
UniGeneiMm.133075.

3D structure databases

ProteinModelPortaliQ8R2H9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231927. 7 interactions.
IntActiQ8R2H9. 7 interactions.
STRINGi10090.ENSMUSP00000057858.

PTM databases

PhosphoSiteiQ8R2H9.

Proteomic databases

MaxQBiQ8R2H9.
PaxDbiQ8R2H9.
PRIDEiQ8R2H9.

Protocols and materials databases

DNASUi237928.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054173; ENSMUSP00000057858; ENSMUSG00000050860.
GeneIDi237928.
KEGGimmu:237928.
UCSCiuc007las.2. mouse.

Organism-specific databases

CTDi162466.
MGIiMGI:2447348. Phospho1.

Phylogenomic databases

eggNOGiKOG3120. Eukaryota.
ENOG4111NAQ. LUCA.
GeneTreeiENSGT00390000007741.
HOGENOMiHOG000231038.
HOVERGENiHBG080058.
InParanoidiQ8R2H9.
KOiK06124.
OMAiSPGMGDL.
PhylomeDBiQ8R2H9.
TreeFamiTF300112.

Enzyme and pathway databases

BRENDAi3.1.3.75. 3474.
ReactomeiR-MMU-1483191. Synthesis of PC.
R-MMU-1483213. Synthesis of PE.

Miscellaneous databases

NextBioi383578.
PROiQ8R2H9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R2H9.
ExpressionAtlasiQ8R2H9. baseline and differential.
GenevisibleiQ8R2H9. MM.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006383. HAD-SF_hydro_IB_PSP-like.
IPR016965. Pase_PHOSPHO-typ.
IPR006384. PyrdxlP_Pase-rel.
[Graphical view]
PfamiPF06888. Put_Phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01489. DKMTPPase-SF. 1 hit.
TIGR01488. HAD-SF-IB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Chromosomal localization of the chicken and mammalian orthologues of the orphan phosphatase PHOSPHO1 gene."
    Houston B., Paton I.R., Burt D.W., Farquharson C.
    Anim. Genet. 33:451-454(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Functional involvement of PHOSPHO1 in matrix vesicle-mediated skeletal mineralization."
    Roberts S., Narisawa S., Harmey D., Millan J.L., Farquharson C.
    J. Bone Miner. Res. 22:617-627(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Brown adipose tissue.

Entry informationi

Entry nameiPHOP1_MOUSE
AccessioniPrimary (citable) accession number: Q8R2H9
Secondary accession number(s): A2A619
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2002
Last modified: May 11, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.