Q8R2H9 (PHOP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoethanolamine/phosphocholine phosphatase
EC=3.1.3.75

Gene names

Name:

Phospho1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization. Ref.3
Catalytic activity	O-phosphoethanolamine + H2O = ethanolamine + phosphate. Phosphocholine + H2O = choline + phosphate.
Cofactor	Magnesium By similarity.
Tissue specificity	Has a 120-fold higher level of expression in bone compared with a range of soft tissues. Ref.3
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.

Ontologies

Keywords
Biological process	Mineral balance
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of bone mineralization Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphocholine phosphatase activity Inferred from electronic annotation. Source: EC phosphoethanolamine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Phosphoethanolamine/phosphocholine phosphatase		PRO_0000068830
Sites
	Active site	32	1	Nucleophile By similarity
	Active site	34	1	Proton donor By similarity
	Metal binding	32	1	Magnesium By similarity
	Metal binding	34	1	Magnesium By similarity
	Metal binding	203	1	Magnesium By similarity
	Binding site	43	1	Substrate By similarity
	Binding site	123	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8R2H9 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 0CEEE66019C0CEA7	FASTA	267	29,911
	10 20 30 40 50 60 MSGCFPAVGL RCLSRDGRMA APGAPRFLLT FDFDETIVDE NSDDSIVRAA PGQQLPESLR 70 80 90 100 110 120 ATYREGYYNE YMQRVFKYLG EQGVRPRDLR AVYETIPLSP GMGDLLQFIA KQGSCFEVIL 130 140 150 160 170 180 ISDANTFGVE SALRAAGHHS LFRRILSNPS GPDARGLLTL RPFHTHSCSR CPANMCKHKV 190 200 210 220 230 240 LSEYLRERAR DGVHFERLFY VGDGANDFCP MGLLAGGDVA FPRRGYPMHR LIQEAQKAEP 250 260 SSFRAHVVPW ETAADVRQHL QQVLKMC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Chromosomal localization of the chicken and mammalian orthologues of the orphan phosphatase PHOSPHO1 gene." Houston B., Paton I.R., Burt D.W., Farquharson C. Anim. Genet. 33:451-454(2002) [PubMed: 12464021] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[3]	"Functional involvement of PHOSPHO1 in matrix vesicle-mediated skeletal mineralization." Roberts S., Narisawa S., Harmey D., Millan J.L., Farquharson C. J. Bone Miner. Res. 22:617-627(2007) [PubMed: 17227223] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ457190 mRNA. Translation: CAD29804.1. AL593858 Genomic DNA. Translation: CAM15510.1.
IPI	IPI00153582.
RefSeq	NP_694744.1. NM_153104.3.
UniGene	Mm.133075.
3D structure databases
ProteinModelPortal	Q8R2H9.
SMR	Q8R2H9. Positions 23-224.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8R2H9.
PTM databases
PhosphoSite	Q8R2H9.
Proteomic databases
PRIDE	Q8R2H9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000054173; ENSMUSP00000057858; ENSMUSG00000050860.
GeneID	237928.
KEGG	mmu:237928.
UCSC	uc007las.2. mouse.
Organism-specific databases
CTD	162466.
MGI	MGI:2447348. Phospho1.
Phylogenomic databases
GeneTree	ENSGT00390000007741.
HOGENOM	HBG319786.
HOVERGEN	HBG080058.
InParanoid	Q8R2H9.
OMA	ASVVPWE.
OrthoDB	EOG4S4PH0.
PhylomeDB	Q8R2H9.
Gene expression databases
ArrayExpress	Q8R2H9.
Bgee	Q8R2H9.
Genevestigator	Q8R2H9.
GermOnline	ENSMUSG00000050860. Mus musculus.
Family and domain databases
InterPro	IPR023214. HAD-like_dom. IPR006383. HAD-SF_hydro_IB_PSP-like. IPR016965. Pase_PHOSPHO-typ. IPR006384. PyrdxlP_Pase-rel. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KO	K06124.
Pfam	PF06888. Put_Phosphatase. 1 hit. [Graphical view]
PIRSF	PIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01489. DKMTPPase-SF. 1 hit. TIGR01488. HAD-SF-IB. 1 hit.
ProtoNet	Search...
Other
NextBio	383578.
SOURCE	Search...

Entry information

Entry name

PHOP1_MOUSE

Accession

Primary (citable) accession number: Q8R2H9
Secondary accession number(s): A2A619

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program