Reviewed, UniProtKB/Swiss-Prot Q8R2H5 (PHLD_RAT)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol-glycan-specific phospholipase D
      Short name=PI-G PLD
    EC=3.1.4.50
Alternative name(s):
    Glycoprotein phospholipase D
    Glycosyl-phosphatidylinositol-specific phospholipase D
      Short name=GPI-specific phospholipase D
      Short name=GPI-PLD

Gene names

Name:

Gpld1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	843 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
Catalytic activity	6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + phosphatidate.
Subunit structure	Monomer Potential.
Subcellular location	Secreted. Note: Associated with the High-Density Lipoproteins (HDL) By similarity.
Post-translational modification	Glycosylated By similarity.
Sequence similarities	Belongs to the GPLD1 family. Contains 7 FG-GAP repeats.

Ontologies

Keywords
Cellular component	HDL Secreted
Domain	Repeat Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	high-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycosylphosphatidylinositol phospholipase D activity Ref.1 Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 843	820	Phosphatidylinositol-glycan-specific phospholipase D		PRO_0000278285
Regions
	Repeat	368 – 429	62	FG-GAP 1
	Repeat	435 – 498	64	FG-GAP 2
	Repeat	500 – 560	61	FG-GAP 3
	Repeat	564 – 625	62	FG-GAP 4
	Repeat	635 – 695	61	FG-GAP 5
	Repeat	707 – 773	67	FG-GAP 6
	Repeat	791 – 843	53	FG-GAP 7
Amino acid modifications
	Glycosylation	94	1	N-linked (GlcNAc...) Potential
	Glycosylation	271	1	N-linked (GlcNAc...) Potential
	Glycosylation	292	1	N-linked (GlcNAc...) Potential
	Glycosylation	308	1	N-linked (GlcNAc...) Potential
	Glycosylation	322	1	N-linked (GlcNAc...) Potential
	Glycosylation	483	1	N-linked (GlcNAc...) Potential
	Glycosylation	502	1	N-linked (GlcNAc...) Potential
	Glycosylation	592	1	N-linked (GlcNAc...) Potential
	Glycosylation	605	1	N-linked (GlcNAc...) Potential
	Glycosylation	661	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8R2H5-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 99964E1C35AB84D6	FASTA	843	93,782
	10 20 30 40 50 60 MSVGRLWSGL LLLLLFFCSR SSSCGLSTHV EIGHRALQFL QLQDGRINYK ELLLEHQDAY 70 80 90 100 110 120 QAGTVFPDAF YPSICKQGKF HEVSESTHWT PFLNASIHYI RENYPLPWEK DTEKLVAFLF 130 140 150 160 170 180 GVTSHMVADV SWHSLGIEQG FLRTMGAIDF YDSYSEAHSA GDFGGDVLSQ FEFNFNYLSR 190 200 210 220 230 240 RWYVPIQDLL RIYDNLYGRK VITKNVIVDC TYLQFLEMHG EMLAVSKLYS TYSTKSPFLV 250 260 270 280 290 300 EQFQDYFLGG LDDMAFWSTN IYRLTSFMLE NGTSDCNLPE NPLFISCDGR KNHTLSSSKV 310 320 330 340 350 360 QKNDFHRNLT MFISKDIRKN LNYTERGVFY STGSWAPESV TFMYQTLERN LRMMFTGNSQ 370 380 390 400 410 420 TALKHVSSPS ASYTLSVPYA RLGWVMASAD LNQDGHGDLV VGAPGYSHPG RFQIGRVYII 430 440 450 460 470 480 YGNDLGLPPI DLDLDKEAHG VLEGFQPSGR FGSALAVLDF NKDGLPDLAV GAPSVGSGQL 490 500 510 520 530 540 TYNGSVYVYY GSQQGRLSSS PNITISCKDT YCNLGWALLA ADADGDGQHD LVISSPFAPG 550 560 570 580 590 600 GGKQRGIVAA FYSHPRRNDK ESLTLDEADW KVNGEEDFSW FGYSLHGVTV ANRSLLLIGS 610 620 630 640 650 660 PTWKNISRMA RSSHQKNQEK KSLGRVYGYF PPNRQREITI SGDKAMGKLG TSLSSGYVRV 670 680 690 700 710 720 NGTLTQVLLV GAPTHDDVSK MAFLTMTLHH GGATRMYELA PEKTQPALFS TFSGDRRFSR 730 740 750 760 770 780 FGSVLHLTDL DDDGLDEIIM AAPLRITDVT SGLLGEEDGR VYIYNGMHTT LGDVTGKCKS 790 800 810 820 830 840 WMTPCPEEKA QYVLISPEAS SRFGSSLVSV RSKERNQVVV AAGRSSWGAR LSGALHVYSF SSD

References

[1]

"Insulin reduces serum glycosylphosphatidylinositol phospholipase D levels in human type I diabetic patients and streptozotocin diabetic rats." Schofield J.N., Stephens J.W., Hurel S.J., Bell K.M., deSouza J.B., Rademacher T.W. Mol. Genet. Metab. 75:154-161(2002) [PubMed: 11855934] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ308109 mRNA. Translation: CAC87069.1.
IPI	IPI00325157.
RefSeq	NP_001093982.1.
UniGene	Rn.18350
3D structure databases
SMR	Q8R2H5. Positions 378-589.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8R2H5.
Genome annotation databases
Ensembl	ENSRNOT00000024196; ENSRNOP00000024196; ENSRNOG00000017702; Rattus norvegicus. [Genome view]
GeneID	291132.
KEGG	rno:291132.
UCSC	AJ308109. rat.
Organism-specific databases
CTD	291132.
RGD	631371. Gpld1.
Phylogenomic databases
eggNOG	roNOG11917.
HOVERGEN	Q8R2H5.
InParanoid	Q8R2H5.
Enzyme and pathway databases
BRENDA	3.1.4.50. 248.
Gene expression databases
ArrayExpress	Q8R2H5.
Genevestigator	Q8R2H5.
Family and domain databases
InterPro	IPR001028. Gprt_PlipaseD. IPR013519. Int_alpha_beta-p. [Graphical view]
PANTHER	PTHR23221:SF2. Gprt_PlipaseD. 1 hit.
PRINTS	PR00718. PHPHLIPASED.
SMART	SM00191. Int_alpha. 5 hits. [Graphical view]
PROSITE	PS51470. FG_GAP. 7 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHLD_RAT

Accession

Primary (citable) accession number: Q8R2H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)