Reviewed, UniProtKB/Swiss-Prot Q8R2G4 (NAR3_MOUSE)

Names and origin

Protein names

Recommended name:
    Ecto-ADP-ribosyltransferase 3
    EC=2.4.2.31
Alternative name(s):
    NAD(P)(+)--arginine ADP-ribosyltransferase 3
    Mono(ADP-ribosyl)transferase 3

Gene names

Name:

Art3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine. NADP+ + protein-L-arginine = nicotinamide + N(omega)-((2'-phospho-ADP)-D-ribosyl)-protein-L-arginine.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity.
Sequence similarities	Belongs to the Arg-specific ADP-ribosyltransferase family.
Sequence caution	The sequence CAC84526.1 differs from that shown. Reason: Miscellaneous discrepancy. 3'extension leads to out-of-frame translation. The sequence CAC84539.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAC84540.1 differs from that shown. Reason: Miscellaneous discrepancy. 3'extension leads to out-of-frame translation. The sequence CAC84541.1 differs from that shown. Reason: Miscellaneous discrepancy. 3'extension leads to out-of-frame translation. The sequence CAC84543.1 differs from that shown. Reason: Miscellaneous discrepancy. 3'extension leads to out-of-frame translation.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	NAD NADP
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Biological process	protein amino acid ADP-ribosylation Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD(P)+-protein-arginine ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 8 (identifier: Q8R2G4-5)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 5 (identifier: Q8R2G4-7)

The sequence of this isoform differs from the canonical sequence as follows:
     271-291: Missing.

Isoform 7 (identifier: Q8R2G4-8)

The sequence of this isoform differs from the canonical sequence as follows:
     321-331: DRSRGKANNPT → A

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 345	319	Ecto-ADP-ribosyltransferase 3		PRO_0000019327
	Propeptide	346 – 371	26	Removed in mature form By similarity		PRO_0000019328
Sites
	Active site	228	1	By similarity
	Binding site	101	1	NAD By similarity
	Binding site	182	1	NAD By similarity
Amino acid modifications
	Lipidation	345	1	GPI-anchor amidated serine By similarity
	Disulfide bond	43 ↔ 255		By similarity
Natural variations
	Alternative sequence	271 – 291	21	Missing in isoform 5.		VSP_036193
	Alternative sequence	321 – 331	11	DRSRGKANNPT → A in isoform 7.		VSP_036199
Experimental info
	Sequence conflict	11 – 15	5	TLLAA → AAGS in CAA69284. Ref.2
	Sequence conflict	348	1	Missing in AAI41070. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 8 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 52F1B373A928182B	FASTA	371	42,015
	10 20 30 40 50 60 MKMGHFEMVT TLLAAAVLMD IFQVKAEVLD MAENAFDDEY LKCKSRMESK YIPQMKREEW 70 80 90 100 110 120 ANDALLRMVW DNAEIQWEAR KAQLFLPRNF KDTYGIALTA YVNEAQEQTS FYHTFSSAVK 130 140 150 160 170 180 MAGLSRRRYI YNFPFKAFHF YLVRALQLLR RPCEKSYKTV VYSTSPDISF TFGEQNQARL 190 200 210 220 230 240 GNFTLAYSAK PETADNQRVL TIQTCFGVAV GKFLNKEDDS VVLIPLSEVF QVSRKGTSND 250 260 270 280 290 300 LVLQSINSTC SYYECAFLGG LKTENCIANA EYIDPRYLYN PDMDNQKLED SGRNNLDPDR 310 320 330 340 350 360 MPEIKVLQTE ENPLLPDEKP DRSRGKANNP TPGLVPGPKS HPSASSGNTL LPSVMASTIL 370 LVASAVNFIE L
	Isoform 5. Checksum: B1F3BB4F6FD80415	FASTA	350	39,414
	10 20 30 40 50 60 MKMGHFEMVT TLLAAAVLMD IFQVKAEVLD MAENAFDDEY LKCKSRMESK YIPQMKREEW 70 80 90 100 110 120 ANDALLRMVW DNAEIQWEAR KAQLFLPRNF KDTYGIALTA YVNEAQEQTS FYHTFSSAVK 130 140 150 160 170 180 MAGLSRRRYI YNFPFKAFHF YLVRALQLLR RPCEKSYKTV VYSTSPDISF TFGEQNQARL 190 200 210 220 230 240 GNFTLAYSAK PETADNQRVL TIQTCFGVAV GKFLNKEDDS VVLIPLSEVF QVSRKGTSND 250 260 270 280 290 300 LVLQSINSTC SYYECAFLGG LKTENCIANA GRNNLDPDRM PEIKVLQTEE NPLLPDEKPD 310 320 330 340 350 RSRGKANNPT PGLVPGPKSH PSASSGNTLL PSVMASTILL VASAVNFIEL
	Isoform 7. Checksum: 17386DF02015D673	FASTA	361	40,889
	10 20 30 40 50 60 MKMGHFEMVT TLLAAAVLMD IFQVKAEVLD MAENAFDDEY LKCKSRMESK YIPQMKREEW 70 80 90 100 110 120 ANDALLRMVW DNAEIQWEAR KAQLFLPRNF KDTYGIALTA YVNEAQEQTS FYHTFSSAVK 130 140 150 160 170 180 MAGLSRRRYI YNFPFKAFHF YLVRALQLLR RPCEKSYKTV VYSTSPDISF TFGEQNQARL 190 200 210 220 230 240 GNFTLAYSAK PETADNQRVL TIQTCFGVAV GKFLNKEDDS VVLIPLSEVF QVSRKGTSND 250 260 270 280 290 300 LVLQSINSTC SYYECAFLGG LKTENCIANA EYIDPRYLYN PDMDNQKLED SGRNNLDPDR 310 320 330 340 350 360 MPEIKVLQTE ENPLLPDEKP APGLVPGPKS HPSASSGNTL LPSVMASTIL LVASAVNFIE L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The family of toxin-related ecto-ADP-ribosyltransferases in humans and the mouse." Glowacki G., Braren R., Firner K., Nissen M., Kuehl M., Reche P., Bazan J.F., Cetkovic-Cvrlje M., Leiter E., Haag F., Koch-Nolte F. Protein Sci. 11:1657-1670(2002) [PubMed: 12070318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 5).
[2]	"Molecular cloning of two ecto-mono(ADP-ribosyl)transferases operating in murine testis." Koch-Nolte F., Firner K., Haag F., Khl M., Thiele H.G. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 8). Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 7). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ311768 Genomic DNA. Translation: CAC84539.1. Sequence problems. AJ311769 mRNA. Translation: CAC84526.1. Sequence problems. AJ311770 mRNA. Translation: CAC84540.1. Sequence problems. AJ311771 mRNA. Translation: CAC84541.1. Sequence problems. AJ311772 mRNA. Translation: CAC84542.1. AJ311773 mRNA. Translation: CAC84543.1. Sequence problems. Y08027 Genomic DNA. Translation: CAA69284.1. BC141069 mRNA. Translation: AAI41070.1.
IPI	IPI00229194. IPI00229195. IPI00229196. IPI00229197. IPI00229198. IPI00880925. IPI00919012.
RefSeq	NP_859417.2.
UniGene	Mm.263514
3D structure databases
HSSP	HSSP built from PDB template 1GXY based on UniProtKB P20974.
ModBase	Search...
Proteomic databases
PRIDE	Q8R2G4.
Genome annotation databases
Ensembl	ENSMUSG00000034842. Mus musculus. [Contig view]
GeneID	109979.
KEGG	mmu:109979.
Organism-specific databases
MGI	MGI:1202729. Art3.
Phylogenomic databases
HOGENOM	Q8R2G4.
HOVERGEN	Q8R2G4.
Enzyme and pathway databases
BRENDA	2.4.2.31. 244.
Gene expression databases
Bgee	Q8R2G4.
CleanEx	MM_ART3.
GermOnline	ENSMUSG00000034842. Mus musculus.
Family and domain databases
InterPro	IPR000768. ART. [Graphical view]
PANTHER	PTHR10339. ART. 1 hit.
Pfam	PF01129. ART. 1 hit. [Graphical view]
PRINTS	PR00970. RIBTRNSFRASE.
ProtoNet	Search...
Other Resources
NextBio	363113.
SOURCE	Search...

Entry information

Entry name

NAR3_MOUSE

Accession

Primary (citable) accession number: Q8R2G4
Secondary accession number(s): B2RUC2 , O54738, Q8R2F9, Q8R2G0, Q8R2G1, Q8R2G2, Q8R2G3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 2002
Last sequence update:	January 20, 2009
Last modified:	June 16, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)