Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Di-N-acetylchitobiase

Gene

Ctbs

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the degradation of asparagine-linked glycoproteins. Hydrolyze of N-acetyl-beta-D-glucosamine (1-4)N-acetylglucosamine chitobiose core from the reducing end of the bond, it requires prior cleavage by glycosylasparaginase (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Di-N-acetylchitobiase (EC:3.2.1.-)
Gene namesi
Name:Ctbs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1921495. Ctbs.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 366344Di-N-acetylchitobiasePRO_0000011962Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence analysis
Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence analysis
Glycosylationi212 – 2121N-linked (GlcNAc...)Sequence analysis
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence analysis
Glycosylationi283 – 2831N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ8R242.
MaxQBiQ8R242.
PeptideAtlasiQ8R242.
PRIDEiQ8R242.

PTM databases

PhosphoSiteiQ8R242.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028189.
CleanExiMM_CTBS.
ExpressionAtlasiQ8R242. baseline and differential.
GenevisibleiQ8R242. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8R242. 1 interaction.
MINTiMINT-4118724.

Structurei

3D structure databases

ProteinModelPortaliQ8R242.
SMRiQ8R242. Positions 100-363.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 18 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00390000012891.
HOGENOMiHOG000007829.
HOVERGENiHBG005486.
InParanoidiQ8R242.
KOiK12310.
OMAiDEQSQIW.
OrthoDBiEOG091G0CA1.
PhylomeDBiQ8R242.
TreeFamiTF332677.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR011583. Chitinase_II.
IPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
PROSITEiPS01095. CHITINASE_18. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R242-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALCGLPEFT LLLLPLLARL SAGDCPCSEA ALCQPIRHRP DFEVFVFDVG
60 70 80 90 100
QKTWKSYDWS QITTVAAFGK YDPELMCYAH SKGARVVLKG DISLKNIIDP
110 120 130 140 150
TFRASWIAQK VDLAKAQYMD GINIDIEQEV NCSSPEYEAL TALVKETTES
160 170 180 190 200
FQREIEGSQV TFDVAWSPKR IDKRCYNYTG IADACDFLFV MSYDEQSQIW
210 220 230 240 250
SECIAAANAP YNQTLTGYID YIKMGISPKK LVMGVPWYGY DYICLNLSKD
260 270 280 290 300
DICTITKVPF RGAPCSDAAG HQVPYKVIMK QVNGSVSGSQ WNKDQQAPYY
310 320 330 340 350
NYKDPAGRFH QVWYDNPQSI SLKAAYVKNY GLRGIGMWNA NCLDYSDDAL
360
AREQTQEMWG ALKPRL
Length:366
Mass (Da):41,295
Last modified:June 7, 2005 - v2
Checksum:i1075F6EAD7AD024A
GO
Isoform 2 (identifier: Q8R242-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     251-304: Missing.

Note: No experimental confirmation available.
Show »
Length:312
Mass (Da):35,307
Checksum:iA0B75A7C0F5BB512
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei251 – 30454Missing in isoform 2. 1 PublicationVSP_013909Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008791 mRNA. Translation: BAB25896.1.
AC124987 Genomic DNA. No translation available.
BC022594 mRNA. Translation: AAH22594.1.
CCDSiCCDS17902.1. [Q8R242-2]
CCDS80048.1. [Q8R242-1]
RefSeqiNP_001280601.1. NM_001293672.1. [Q8R242-1]
NP_083112.1. NM_028836.4. [Q8R242-2]
UniGeneiMm.45396.

Genome annotation databases

EnsembliENSMUST00000029840; ENSMUSP00000029840; ENSMUSG00000028189. [Q8R242-2]
ENSMUST00000061937; ENSMUSP00000059167; ENSMUSG00000028189. [Q8R242-1]
GeneIDi74245.
KEGGimmu:74245.
UCSCiuc008rrg.2. mouse. [Q8R242-2]
uc008rrh.2. mouse. [Q8R242-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008791 mRNA. Translation: BAB25896.1.
AC124987 Genomic DNA. No translation available.
BC022594 mRNA. Translation: AAH22594.1.
CCDSiCCDS17902.1. [Q8R242-2]
CCDS80048.1. [Q8R242-1]
RefSeqiNP_001280601.1. NM_001293672.1. [Q8R242-1]
NP_083112.1. NM_028836.4. [Q8R242-2]
UniGeneiMm.45396.

3D structure databases

ProteinModelPortaliQ8R242.
SMRiQ8R242. Positions 100-363.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R242. 1 interaction.
MINTiMINT-4118724.

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

PTM databases

PhosphoSiteiQ8R242.

Proteomic databases

EPDiQ8R242.
MaxQBiQ8R242.
PeptideAtlasiQ8R242.
PRIDEiQ8R242.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029840; ENSMUSP00000029840; ENSMUSG00000028189. [Q8R242-2]
ENSMUST00000061937; ENSMUSP00000059167; ENSMUSG00000028189. [Q8R242-1]
GeneIDi74245.
KEGGimmu:74245.
UCSCiuc008rrg.2. mouse. [Q8R242-2]
uc008rrh.2. mouse. [Q8R242-1]

Organism-specific databases

CTDi1486.
MGIiMGI:1921495. Ctbs.

Phylogenomic databases

GeneTreeiENSGT00390000012891.
HOGENOMiHOG000007829.
HOVERGENiHBG005486.
InParanoidiQ8R242.
KOiK12310.
OMAiDEQSQIW.
OrthoDBiEOG091G0CA1.
PhylomeDBiQ8R242.
TreeFamiTF332677.

Miscellaneous databases

PROiQ8R242.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028189.
CleanExiMM_CTBS.
ExpressionAtlasiQ8R242. baseline and differential.
GenevisibleiQ8R242. MM.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR011583. Chitinase_II.
IPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
PROSITEiPS01095. CHITINASE_18. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDIAC_MOUSE
AccessioniPrimary (citable) accession number: Q8R242
Secondary accession number(s): Q9D7V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.