Q8R207 (SPTSA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine palmitoyltransferase small subunit A

Alternative name(s):
Small subunit of serine palmitoyltransferase A
Short name=ssSPTa

Gene names

Name:	Sptssa
Synonyms:	Ssspta

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	71 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Stimulates the activity of serine palmitoyltransferase (SPT). The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA By similarity.
Pathway	Lipid metabolism; sphingolipid metabolism.
Subunit structure	Interacts with SPTLC1; the interaction is direct. Component of the serine palmitoyltransferase (SPT) complex, composed of SPTLC1, either SPTLC2 or SPTLC3, and either SPTSSA or SPTSSB By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the SPTSS family. SPTSSA subfamily.
Caution	It is uncertain whether Met-1 or Met-4 is the initiator.
Sequence caution	The sequence AAH22674.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC25023.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC25026.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC25233.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC25555.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW serine C-palmitoyltransferase complex Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 71	71	Serine palmitoyltransferase small subunit A		PRO_0000089950
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 29	17	Helical; Potential
	Topological domain	30 – 34	5	Lumenal Potential
	Transmembrane	35 – 57	23	Helical; Potential
	Topological domain	58 – 71	14	Cytoplasmic Potential
Experimental info
	Sequence conflict	9	1	A → S in BAC25026. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8R207 [UniParc]. Last modified May 26, 2009. Version 2. Checksum: ED4F189EB101703C	FASTA	71	8,452
	10 20 30 40 50 60 MAGMALARAW KQMSWFYYQY LLVTALYMLE PWERTVFNSM LVSVVGMALY TGYVFMPQHI 70 MAILHYFEIV Q

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung and Stomach.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK003171 mRNA. Translation: BAC25023.1. Different initiation. AK003275 mRNA. Translation: BAC25026.1. Different initiation. AK008904 mRNA. Translation: BAC25233.1. Different initiation. AK018380 mRNA. Translation: BAC25555.1. Different initiation. BC022674 mRNA. Translation: AAH22674.1. Different initiation.
IPI	IPI00153420.
RefSeq	NP_598815.2. NM_134054.2.
UniGene	Mm.485026.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8R207.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000056228; ENSMUSP00000053671; ENSMUSG00000044408.
GeneID	104725.
KEGG	mmu:104725.
UCSC	uc007nnt.1. mouse.
Organism-specific databases
MGI	MGI:1913399. 1110002B05Rik.
Phylogenomic databases
eggNOG	roNOG17452.
GeneTree	ENSGT00390000002766.
InParanoid	Q8R207.
OMA	ILAILHY.
OrthoDB	EOG4G7C12.
Gene expression databases
ArrayExpress	Q8R207.
Bgee	Q8R207.
CleanEx	MM_1110002B05RIK.
Genevestigator	Q8R207.
GermOnline	ENSMUSG00000044408. Mus musculus.
Family and domain databases
InterPro	IPR024512. Ser_palmitoyltrfase_ssu-like. [Graphical view]
Pfam	PF11779. DUF3317. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	357240.
SOURCE	Search...

Entry information

Entry name

SPTSA_MOUSE

Accession

Primary (citable) accession number: Q8R207
Secondary accession number(s): Q8BHT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	May 26, 2009
Last modified:	October 19, 2011
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program