Q8R1W2 (GSG1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Germ cell-specific gene 1 protein

Alternative name(s):
Germ cell-associated protein 1

Gene names

Name:

Gsg1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May cause the redistribution of PAPOLB from the cytosol to the endoplasmic reticulum. Ref.6
Subunit structure	Interacts with PAPOLB. Ref.6
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Colocalizes with PAPOLB in the endoplasmic reticulum. Ref.6
Tissue specificity	Expressed in spermatogenic cells (at protein level). Expressed in germ cells within the testis from day 21 onwards. Ref.1 Ref.6
Sequence similarities	Belongs to the GSG1 family.
Sequence caution	The sequence AAH23009.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA37087.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.6. Source: MGI endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8R1W2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8R1W2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     125-125: R → RGEKGLLEFATLQGSCHPTLRFGGEWLMEKASLLHLPWGPVAKVF

Isoform 3 (identifier: Q8R1W2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-107: Missing.
     108-125: EPGEKCRRFIELTPPAQR → MEKASLLHLPWGPVAKVF

Isoform 4 (identifier: Q8R1W2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MAKM → M
     125-125: R → RGEKGLLEFATLQGSCHPTLRFGGEWLMEKASLLHLPWGPVAKVF

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Germ cell-specific gene 1 protein		PRO_0000329462
Regions
	Transmembrane	15 – 35	21	Helical; Potential
	Transmembrane	127 – 147	21	Helical; Potential
	Transmembrane	164 – 184	21	Helical; Potential
	Transmembrane	208 – 228	21	Helical; Potential
Natural variations
	Alternative sequence	1 – 107	107	Missing in isoform 3.		VSP_032999
	Alternative sequence	1 – 4	4	MAKM → M in isoform 4.		VSP_032998
	Alternative sequence	108 – 125	18	EPGEK…PPAQR → MEKASLLHLPWGPVAKVF in isoform 3.		VSP_033000
	Alternative sequence	125	1	R → RGEKGLLEFATLQGSCHPTL RFGGEWLMEKASLLHLPWGP VAKVF in isoform 2 and isoform 4.		VSP_033001
Experimental info
	Sequence conflict	178	1	M → I in BAC40258. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 9AB3A42C147F2B90	FASTA	324	36,108
	10 20 30 40 50 60 MAKMEFQKGS SDQRTFISAI LNMLSLGLST ASLLSSEWFV GTQKVPKPLC GQSLAAKCFD 70 80 90 100 110 120 MPMSLDGGIA NTSAQEVVQY TWETGDDRFS FLAFRSGMWL SCEETMEEPG EKCRRFIELT 130 140 150 160 170 180 PPAQRWLSLG AQTAYIGLQL ISFLLLLTDL LLTTNPGCGL KLSAFAAVSL VLSGLLGMVA 190 200 210 220 230 240 HMLYSQVFQA TANLGPEDWR PHSWNYGWAF YTAWVSFTCC MASAVTTFNM YTRMVLEFKC 250 260 270 280 290 300 RHSKSFNTNP SCLAQHHRCF LPPPLTCTTH AGEPLSSCHQ YPSHPIRSVS EAIDLYSALQ 310 320 DKEFQQGISQ ELKEVVEPSV EEQR
	Isoform 2 [UniParc]. Checksum: 0ADF2ADD7DC5E4C3	FASTA	368	40,943
	10 20 30 40 50 60 MAKMEFQKGS SDQRTFISAI LNMLSLGLST ASLLSSEWFV GTQKVPKPLC GQSLAAKCFD 70 80 90 100 110 120 MPMSLDGGIA NTSAQEVVQY TWETGDDRFS FLAFRSGMWL SCEETMEEPG EKCRRFIELT 130 140 150 160 170 180 PPAQRGEKGL LEFATLQGSC HPTLRFGGEW LMEKASLLHL PWGPVAKVFW LSLGAQTAYI 190 200 210 220 230 240 GLQLISFLLL LTDLLLTTNP GCGLKLSAFA AVSLVLSGLL GMVAHMLYSQ VFQATANLGP 250 260 270 280 290 300 EDWRPHSWNY GWAFYTAWVS FTCCMASAVT TFNMYTRMVL EFKCRHSKSF NTNPSCLAQH 310 320 330 340 350 360 HRCFLPPPLT CTTHAGEPLS SCHQYPSHPI RSVSEAIDLY SALQDKEFQQ GISQELKEVV EPSVEEQR
	Isoform 3 [UniParc]. Checksum: C9775ACA6478AF1B	FASTA	217	24,215
	10 20 30 40 50 60 MEKASLLHLP WGPVAKVFWL SLGAQTAYIG LQLISFLLLL TDLLLTTNPG CGLKLSAFAA 70 80 90 100 110 120 VSLVLSGLLG MVAHMLYSQV FQATANLGPE DWRPHSWNYG WAFYTAWVSF TCCMASAVTT 130 140 150 160 170 180 FNMYTRMVLE FKCRHSKSFN TNPSCLAQHH RCFLPPPLTC TTHAGEPLSS CHQYPSHPIR 190 200 210 SVSEAIDLYS ALQDKEFQQG ISQELKEVVE PSVEEQR
	Isoform 4 [UniParc]. Checksum: 2D0A0AEC0EA971FD	FASTA	365	40,613
	10 20 30 40 50 60 MEFQKGSSDQ RTFISAILNM LSLGLSTASL LSSEWFVGTQ KVPKPLCGQS LAAKCFDMPM 70 80 90 100 110 120 SLDGGIANTS AQEVVQYTWE TGDDRFSFLA FRSGMWLSCE ETMEEPGEKC RRFIELTPPA 130 140 150 160 170 180 QRGEKGLLEF ATLQGSCHPT LRFGGEWLME KASLLHLPWG PVAKVFWLSL GAQTAYIGLQ 190 200 210 220 230 240 LISFLLLLTD LLLTTNPGCG LKLSAFAAVS LVLSGLLGMV AHMLYSQVFQ ATANLGPEDW 250 260 270 280 290 300 RPHSWNYGWA FYTAWVSFTC CMASAVTTFN MYTRMVLEFK CRHSKSFNTN PSCLAQHHRC 310 320 330 340 350 360 FLPPPLTCTT HAGEPLSSCH QYPSHPIRSV SEAIDLYSAL QDKEFQQGIS QELKEVVEPS VEEQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of cDNA clones specifically expressed in testicular germ cells." Tanaka H., Yoshimura Y., Nishina Y., Nozaki M., Nojima H., Nishimune Y. FEBS Lett. 355:4-10(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Testis.
[2]	"Mapping of six germ-cell-specific genes to mouse chromosomes." Matsui M., Ichihara H., Kobayashi S., Tanaka H., Tsuchida J., Nozaki M., Yoshimura Y., Nojima H., Rochelle J.M., Nishimune Y., Taketo M.M., Seldin M.F. Mamm. Genome 8:873-874(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4). Strain: C57BL/6. Tissue: Testis.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6J and NOD. Tissue: Testis and Thymus.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Eye.
[6]	"Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to the endoplasmic reticulum through protein-protein interactions." Choi H.-S., Lee S.-H., Kim H., Lee Y. FEBS Lett. 582:1203-1209(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PAPOLB, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87325 mRNA. Translation: BAA37087.1. Frameshift. AK006326 mRNA. Translation: BAB24527.1. AK088285 mRNA. Translation: BAC40258.1. AC122820 Genomic DNA. No translation available. BC023009 mRNA. Translation: AAH23009.1. Different initiation.
IPI	IPI00153480. IPI00828917. IPI00857678. IPI00890842.
RefSeq	NP_001074021.1. NM_001080552.1. NP_001074022.1. NM_001080553.1. NP_034482.2. NM_010352.2.
UniGene	Mm.272306.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-6167922.
PTM databases
PhosphoSite	Q8R1W2.
Proteomic databases
PRIDE	Q8R1W2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000087729; ENSMUSP00000085022; ENSMUSG00000030206. ENSMUST00000111910; ENSMUSP00000107541; ENSMUSG00000030206. ENSMUST00000111911; ENSMUSP00000107542; ENSMUSG00000030206.
GeneID	14840.
KEGG	mmu:14840.
UCSC	uc009elj.1. mouse. uc009elk.1. mouse.
Organism-specific databases
CTD	83445.
MGI	MGI:1194499. Gsg1.
Phylogenomic databases
eggNOG	NOG47621.
GeneTree	ENSGT00390000011933.
HOGENOM	HOG000112826.
HOVERGEN	HBG069545.
OMA	WNYGWAF.
OrthoDB	EOG42822J.
Gene expression databases
ArrayExpress	Q8R1W2.
Bgee	Q8R1W2.
Genevestigator	Q8R1W2.
Family and domain databases
InterPro	IPR012478. GSG-1. [Graphical view]
Pfam	PF07803. GSG-1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	287055.
SOURCE	Search...

Entry information

Entry name

GSG1_MOUSE

Accession

Primary (citable) accession number: Q8R1W2
Secondary accession number(s): Q8C2N5, Q9D9Z3, Q9Z1H7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	April 3, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program