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Protein

Transmembrane emp24 domain-containing protein 4

Gene

Tmed4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in vesicular protein trafficking, mainly in the early secretory pathway. Involved in the maintenance of the Golgi apparatus. Appears to play a role in the biosynthesis of secreted cargo including processing. Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 4
Alternative name(s):
Endoplasmic reticulum stress-response protein 25
Short name:
ERS25
p24 family protein alpha-3
Short name:
p24alpha3
p26
Gene namesi
Name:Tmed4
Synonyms:Ers25
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1915070. Tmed4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 194165LumenalSequence analysisAdd
BLAST
Transmembranei195 – 21218HelicalSequence analysisAdd
BLAST
Topological domaini213 – 22715CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Chaini30 – 227198Transmembrane emp24 domain-containing protein 4PRO_0000010388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ8R1V4.
MaxQBiQ8R1V4.
PaxDbiQ8R1V4.
PeptideAtlasiQ8R1V4.
PRIDEiQ8R1V4.

PTM databases

iPTMnetiQ8R1V4.
PhosphoSiteiQ8R1V4.

Expressioni

Inductioni

By brefeldin A, oxidative stress and heat shock, but not by tunicamycin, hypersomotic stress or serum starvation.1 Publication

Gene expression databases

BgeeiQ8R1V4.
ExpressionAtlasiQ8R1V4. baseline and differential.
GenevisibleiQ8R1V4. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8R1V4. 3 interactions.
MINTiMINT-4997787.
STRINGi10090.ENSMUSP00000004508.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 13799GOLDPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili147 – 17630Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi220 – 2278COPI vesicle coat-bindingSequence analysis
Motifi220 – 2212COPII vesicle coat-bindingSequence analysis

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated
Contains 1 GOLD domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1690. Eukaryota.
ENOG410XPUW. LUCA.
GeneTreeiENSGT00430000030848.
HOGENOMiHOG000160228.
HOVERGENiHBG105357.
InParanoidiQ8R1V4.
OMAiLGMHVEI.
OrthoDBiEOG74BJT2.
PhylomeDBiQ8R1V4.
TreeFamiTF314123.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R1V4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVGVGPLQ GMVRFGLLVL TVCAACARGL YFHIGETEKR CFIEEIPDET
60 70 80 90 100
MVIGNYRTQM WDKQKEVFLP STPGLGMHVE VKDPDGKVVL SRQYGSEGRF
110 120 130 140 150
TFTSHTPGDH QICLHSNSTR MALFAGGKLR VHLDIQVGEH ANNYPEIAAK
160 170 180 190 200
DKLTELQLRA RQLLDQVEQI QKEQDYQRYR EERFRLTSES TNQRVLWWSI
210 220
AQTVILILTG IWQMRHLKSF FEAKKLV
Length:227
Mass (Da):26,022
Last modified:June 1, 2002 - v1
Checksum:i38BF0C8475C954BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF368921 mRNA. Translation: AAM20821.1.
AK078425 mRNA. Translation: BAC37268.1.
AK158876 mRNA. Translation: BAE34708.1.
AL607152 Genomic DNA. Translation: CAI24400.1.
BC023041 mRNA. Translation: AAH23041.1.
CCDSiCCDS24415.1.
RefSeqiNP_598781.1. NM_134020.1.
UniGeneiMm.254495.
Mm.471082.

Genome annotation databases

EnsembliENSMUST00000004508; ENSMUSP00000004508; ENSMUSG00000004394.
GeneIDi103694.
KEGGimmu:103694.
UCSCiuc007hye.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF368921 mRNA. Translation: AAM20821.1.
AK078425 mRNA. Translation: BAC37268.1.
AK158876 mRNA. Translation: BAE34708.1.
AL607152 Genomic DNA. Translation: CAI24400.1.
BC023041 mRNA. Translation: AAH23041.1.
CCDSiCCDS24415.1.
RefSeqiNP_598781.1. NM_134020.1.
UniGeneiMm.254495.
Mm.471082.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R1V4. 3 interactions.
MINTiMINT-4997787.
STRINGi10090.ENSMUSP00000004508.

PTM databases

iPTMnetiQ8R1V4.
PhosphoSiteiQ8R1V4.

Proteomic databases

EPDiQ8R1V4.
MaxQBiQ8R1V4.
PaxDbiQ8R1V4.
PeptideAtlasiQ8R1V4.
PRIDEiQ8R1V4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004508; ENSMUSP00000004508; ENSMUSG00000004394.
GeneIDi103694.
KEGGimmu:103694.
UCSCiuc007hye.1. mouse.

Organism-specific databases

CTDi222068.
MGIiMGI:1915070. Tmed4.

Phylogenomic databases

eggNOGiKOG1690. Eukaryota.
ENOG410XPUW. LUCA.
GeneTreeiENSGT00430000030848.
HOGENOMiHOG000160228.
HOVERGENiHBG105357.
InParanoidiQ8R1V4.
OMAiLGMHVEI.
OrthoDBiEOG74BJT2.
PhylomeDBiQ8R1V4.
TreeFamiTF314123.

Miscellaneous databases

PROiQ8R1V4.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R1V4.
ExpressionAtlasiQ8R1V4. baseline and differential.
GenevisibleiQ8R1V4. MM.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Zhang W., Xie Z., Cao X.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Visual cortex and Wolffian duct.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  5. "Novel oxidative stress-responsive gene ERS25 functions as a regulator of the heat-shock and cell death response."
    Hwang S.O., Boswell S.A., Seo J.-S., Lee S.W.
    J. Biol. Chem. 283:13063-13069(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiTMED4_MOUSE
AccessioniPrimary (citable) accession number: Q8R1V4
Secondary accession number(s): Q3TY55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: June 1, 2002
Last modified: July 6, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.