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Protein

Sodium-coupled neutral amino acid transporter 4

Gene

Slc38a4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-dependent amino acid transporter. Mediates electrogenic symport of neutral amino acids and sodium ions. Has a broad specificity, with a preference for Ala, followed by Ser, His, Gly, Cys, Asn, Thr, Pro, Gln and Met. May mediate sodium-independent transport of cationic amino acids, such as Arg and Lys. Amino acid uptake is pH-dependent, with lower transport activities at pH 6.5, intermediate at pH 7.0 and highest between pH 7.5 and 8.5.1 Publication

pH dependencei

Optimum pH is 7.5-8.5.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-MMU-352230. Amino acid transport across the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-coupled neutral amino acid transporter 4
Alternative name(s):
Amino acid transporter A3
Na(+)-coupled neutral amino acid transporter 4
Solute carrier family 38 member 4
System A amino acid transporter 3
Gene namesi
Name:Slc38a4
Synonyms:Ata3, Nat3, Snat4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1916604. Slc38a4.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 104ExtracellularSequence analysisAdd BLAST104
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Topological domaini126 – 151CytoplasmicSequence analysisAdd BLAST26
Transmembranei152 – 172HelicalSequence analysisAdd BLAST21
Topological domaini173 – 195ExtracellularSequence analysisAdd BLAST23
Transmembranei196 – 216HelicalSequence analysisAdd BLAST21
Topological domaini217 – 220CytoplasmicSequence analysis4
Transmembranei221 – 241HelicalSequence analysisAdd BLAST21
Topological domaini242 – 332ExtracellularSequence analysisAdd BLAST91
Transmembranei333 – 353HelicalSequence analysisAdd BLAST21
Topological domaini354 – 369CytoplasmicSequence analysisAdd BLAST16
Transmembranei370 – 390HelicalSequence analysisAdd BLAST21
Topological domaini391 – 411ExtracellularSequence analysisAdd BLAST21
Transmembranei412 – 432HelicalSequence analysisAdd BLAST21
Topological domaini433 – 453CytoplasmicSequence analysisAdd BLAST21
Transmembranei454 – 474HelicalSequence analysisAdd BLAST21
Topological domaini475 – 476ExtracellularSequence analysis2
Transmembranei477 – 497HelicalSequence analysisAdd BLAST21
Topological domaini498 – 514CytoplasmicSequence analysisAdd BLAST17
Transmembranei515 – 535HelicalSequence analysisAdd BLAST21
Topological domaini536 – 547ExtracellularSequence analysisAdd BLAST12

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi18C → A: No significant effect on alanine uptake. 1 Publication1
Mutagenesisi232C → A: 40% decrease in alanine uptake. 1 Publication1
Mutagenesisi249C → A: Abolishes transport activity. 1 Publication1
Mutagenesisi321C → A: Abolishes transport activity. 1 Publication1
Mutagenesisi345C → A: No significant effect on alanine uptake. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002478611 – 547Sodium-coupled neutral amino acid transporter 4Add BLAST547

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49PhosphoserineBy similarity1
Disulfide bondi249 ↔ 3211 Publication
Glycosylationi260N-linked (GlcNAc...)Sequence analysis1
Glycosylationi264N-linked (GlcNAc...)Sequence analysis1
Glycosylationi276N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The disulfide bond plays an important role in substrate transport, but has no effect on trafficking to the cell surface.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8R1S9.
PaxDbiQ8R1S9.
PeptideAtlasiQ8R1S9.
PRIDEiQ8R1S9.

PTM databases

iPTMnetiQ8R1S9.
PhosphoSitePlusiQ8R1S9.

Expressioni

Tissue specificityi

Detected in liver, in hepatocytes surrounding the central vein. Not detected in heart, kidney, brain, lung, small intestine, spleen and thymus.1 Publication

Gene expression databases

BgeeiENSMUSG00000022464.
CleanExiMM_NAT3.
MM_SLC38A4.
GenevisibleiQ8R1S9. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8R1S9. 1 interactor.
MINTiMINT-4118642.
STRINGi10090.ENSMUSP00000023101.

Structurei

3D structure databases

ProteinModelPortaliQ8R1S9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1305. Eukaryota.
COG0814. LUCA.
GeneTreeiENSGT00760000119147.
HOGENOMiHOG000013088.
HOVERGENiHBG059571.
InParanoidiQ8R1S9.
KOiK14991.
OMAiNTLPMHV.
OrthoDBiEOG091G0GP8.
PhylomeDBiQ8R1S9.
TreeFamiTF328787.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R1S9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPMELNNVS IEPDGDSCSG DSIQDSYTGM ENSDKDAMNS QFANEDAESQ
60 70 80 90 100
KFLTNGFLGK KKLADYADEH HPGMTSFGMS SFNLSNAIMG SGILGLSYAM
110 120 130 140 150
ANTGIILFII MLLTVAILSL YSVHLLLKTA KEGGSLIYEK LGEKAFGWPG
160 170 180 190 200
KIGAFISITM QNIGAMSSYL FIIKYELPEV IRAFMGLEEN TGEWYLNGNY
210 220 230 240 250
LVLFVSVGII LPLSLLKNLG YLGYTSGFSL SCMVFFVSVV IYKKFQIPCP
260 270 280 290 300
LPALDHNNGN LTFNNTLPIH MISLPNDSES SGVNFMMDYA HHNPAGLDEK
310 320 330 340 350
QVAGPLHSNG VEYEAQGAEK CQPKYFVFNS RTAYAIPILA FAFVCHPEVL
360 370 380 390 400
PIYSELKDRS RRKMQTVSNI SISGMLVMYL LAALFGYLSF YGDVEDELLH
410 420 430 440 450
AYSKVYTFDT ALLMVRLAVL VAVTLTVPIV LFPIRTSVIT LLFPRKPFSW
460 470 480 490 500
LKHFGIAAII IALNNILVIL VPTIKYIFGF IGASSATMLI FILPAAFYLK
510 520 530 540
LVKKEPLRSP QKIGALVFLV TGIIFMMGSM ALIILDWIYN PPNPNHH
Length:547
Mass (Da):60,464
Last modified:June 1, 2002 - v1
Checksum:i7A3B615B6CA09D08
GO

Sequence cautioni

The sequence BAE37721 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90G → S in BAE41099 (PubMed:16141072).Curated1
Sequence conflicti143E → G in BAE37721 (PubMed:16141072).Curated1
Sequence conflicti316Q → K in BAE37721 (PubMed:16141072).Curated1
Sequence conflicti345C → R in BAB84091 (Ref. 2) Curated1
Sequence conflicti546 – 547HH → PP in BAE39606 (PubMed:16141072).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY027919 mRNA. Translation: AAK21967.1.
AB055004 mRNA. Translation: BAB84091.1.
AK051023 mRNA. Translation: BAC34500.1.
AK164292 mRNA. Translation: BAE37721.1. Different initiation.
AK167539 mRNA. Translation: BAE39606.1.
AK169349 mRNA. Translation: BAE41099.1.
BC024072 mRNA. Translation: AAH24072.1.
BC024123 mRNA. Translation: AAH24123.1.
BC031717 mRNA. Translation: AAH31717.1.
CCDSiCCDS27779.1.
RefSeqiNP_081328.2. NM_027052.3.
XP_006521409.1. XM_006521346.3.
XP_006521411.1. XM_006521348.3.
XP_006521412.1. XM_006521349.3.
XP_006521413.1. XM_006521350.3.
XP_006521414.1. XM_006521351.2.
UniGeneiMm.250980.

Genome annotation databases

EnsembliENSMUST00000023101; ENSMUSP00000023101; ENSMUSG00000022464.
ENSMUST00000166223; ENSMUSP00000127676; ENSMUSG00000022464.
GeneIDi69354.
KEGGimmu:69354.
UCSCiuc007xko.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY027919 mRNA. Translation: AAK21967.1.
AB055004 mRNA. Translation: BAB84091.1.
AK051023 mRNA. Translation: BAC34500.1.
AK164292 mRNA. Translation: BAE37721.1. Different initiation.
AK167539 mRNA. Translation: BAE39606.1.
AK169349 mRNA. Translation: BAE41099.1.
BC024072 mRNA. Translation: AAH24072.1.
BC024123 mRNA. Translation: AAH24123.1.
BC031717 mRNA. Translation: AAH31717.1.
CCDSiCCDS27779.1.
RefSeqiNP_081328.2. NM_027052.3.
XP_006521409.1. XM_006521346.3.
XP_006521411.1. XM_006521348.3.
XP_006521412.1. XM_006521349.3.
XP_006521413.1. XM_006521350.3.
XP_006521414.1. XM_006521351.2.
UniGeneiMm.250980.

3D structure databases

ProteinModelPortaliQ8R1S9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R1S9. 1 interactor.
MINTiMINT-4118642.
STRINGi10090.ENSMUSP00000023101.

PTM databases

iPTMnetiQ8R1S9.
PhosphoSitePlusiQ8R1S9.

Proteomic databases

MaxQBiQ8R1S9.
PaxDbiQ8R1S9.
PeptideAtlasiQ8R1S9.
PRIDEiQ8R1S9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023101; ENSMUSP00000023101; ENSMUSG00000022464.
ENSMUST00000166223; ENSMUSP00000127676; ENSMUSG00000022464.
GeneIDi69354.
KEGGimmu:69354.
UCSCiuc007xko.1. mouse.

Organism-specific databases

CTDi55089.
MGIiMGI:1916604. Slc38a4.

Phylogenomic databases

eggNOGiKOG1305. Eukaryota.
COG0814. LUCA.
GeneTreeiENSGT00760000119147.
HOGENOMiHOG000013088.
HOVERGENiHBG059571.
InParanoidiQ8R1S9.
KOiK14991.
OMAiNTLPMHV.
OrthoDBiEOG091G0GP8.
PhylomeDBiQ8R1S9.
TreeFamiTF328787.

Enzyme and pathway databases

ReactomeiR-MMU-352230. Amino acid transport across the plasma membrane.

Miscellaneous databases

ChiTaRSiSlc38a4. mouse.
PROiQ8R1S9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022464.
CleanExiMM_NAT3.
MM_SLC38A4.
GenevisibleiQ8R1S9. MM.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS38A4_MOUSE
AccessioniPrimary (citable) accession number: Q8R1S9
Secondary accession number(s): Q3TEZ9
, Q3TJ89, Q3TPL5, Q8VIE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Imprinted gene expressed from the paternal allele in blastocysts.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.