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Protein

Metastasis suppressor protein 1

Gene

Mtss1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the nucleation of actin filaments in vitro.1 Publication

GO - Molecular functioni

  • actin binding Source: MGI
  • actin filament binding Source: MGI
  • actin monomer binding Source: UniProtKB
  • receptor binding Source: UniProtKB

GO - Biological processi

  • actin filament organization Source: MGI
  • actin filament polymerization Source: MGI
  • adherens junction maintenance Source: MGI
  • bone mineralization Source: MGI
  • cellular response to fluid shear stress Source: UniProtKB
  • epithelial cell proliferation involved in renal tubule morphogenesis Source: UniProtKB
  • glomerulus morphogenesis Source: UniProtKB
  • in utero embryonic development Source: MGI
  • magnesium ion homeostasis Source: MGI
  • muscle organ development Source: UniProtKB
  • negative regulation of epithelial cell proliferation Source: UniProtKB
  • nephron tubule epithelial cell differentiation Source: UniProtKB
  • nervous system development Source: UniProtKB
  • plasma membrane organization Source: InterPro
  • positive regulation of actin filament bundle assembly Source: MGI
  • renal system development Source: MGI
  • renal tubule morphogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Metastasis suppressor protein 1
Alternative name(s):
Missing in metastasis protein
Gene namesi
Name:Mtss1
Synonyms:Mim
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2384818. Mtss1.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: UniProtKB
  • adherens junction Source: MGI
  • cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi746K → A: Loss of actin-binding. 1 Publication1
Mutagenesisi747K → A: Loss of actin-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000966401 – 759Metastasis suppressor protein 1Add BLAST759

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei262PhosphothreonineCombined sources1
Modified residuei265PhosphoserineCombined sources1
Modified residuei266PhosphoserineCombined sources1
Modified residuei275PhosphoserineCombined sources1
Modified residuei326PhosphoserineCombined sources1
Modified residuei429PhosphothreonineCombined sources1
Modified residuei607PhosphothreonineCombined sources1
Modified residuei648PhosphoserineCombined sources1
Modified residuei651PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8R1S4.
PRIDEiQ8R1S4.

PTM databases

iPTMnetiQ8R1S4.
PhosphoSitePlusiQ8R1S4.

Expressioni

Tissue specificityi

Strongly expressed in the developing neurons and skeletal and cardiac muscles in embryos. Strongly expressed also in liver, outer layers of the kidney, and in the Purkinje cells of the brain.1 Publication

Gene expression databases

CleanExiMM_MTSS1.

Interactioni

Subunit structurei

Binds to actin.

GO - Molecular functioni

  • actin binding Source: MGI
  • actin filament binding Source: MGI
  • actin monomer binding Source: UniProtKB
  • receptor binding Source: UniProtKB

Protein-protein interaction databases

DIPiDIP-29271N.
STRINGi10090.ENSMUSP00000079239.

Structurei

Secondary structure

1759
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 24Combined sources24
Helixi27 – 66Combined sources40
Helixi71 – 103Combined sources33
Helixi105 – 150Combined sources46
Helixi154 – 156Combined sources3
Turni157 – 159Combined sources3
Helixi162 – 214Combined sources53
Helixi215 – 219Combined sources5
Helixi220 – 233Combined sources14
Helixi242 – 244Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D1LX-ray1.85A/B1-250[»]
ProteinModelPortaliQ8R1S4.
SMRiQ8R1S4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R1S4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 254IMDPROSITE-ProRule annotationAdd BLAST254
Domaini731 – 748WH2PROSITE-ProRule annotationCuratedAdd BLAST18

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili108 – 157Sequence analysisAdd BLAST50

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi242 – 363Ser-richAdd BLAST122
Compositional biasi612 – 730Pro-richAdd BLAST119

Domaini

The WH2 motif at the C-terminus binds to actin monomers.1 Publication

Sequence similaritiesi

Belongs to the MTSS1 family.Curated
Contains 1 IMD (IRSp53/MIM homology) domain.PROSITE-ProRule annotation
Contains 1 WH2 domain.PROSITE-ProRule annotationCurated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGVU. Eukaryota.
ENOG410XRT4. LUCA.
HOGENOMiHOG000113691.
HOVERGENiHBG052530.
InParanoidiQ8R1S4.
KOiK20128.
PhylomeDBiQ8R1S4.

Family and domain databases

InterProiIPR013606. I-BAR_dom.
IPR027682. MIM.
IPR030127. MIM/ABBA.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR15708. PTHR15708. 1 hit.
PTHR15708:SF10. PTHR15708:SF10. 1 hit.
PfamiPF08397. IMD. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
PROSITEiPS51338. IMD. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1Curated (identifier: Q8R1S4-1) [UniParc]FASTAAdd to basket
Also known as: Long1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAVIEKECS ALGGLFQTII SDMKGSYPVW EDFINKAGKL QSQLRTTVVA
60 70 80 90 100
AAAFLDAFQK VADMATNTRG GTREIGSALT RMCMRHRSIE AKLRQFSSAL
110 120 130 140 150
IDCLINPLQE QMEEWKKVAN QLDKDHAKEY KKARQEIKNK SSDTLKLQKK
160 170 180 190 200
AKKVDAQGRG DIQPQLDSAL QDVNDKYLLL EETEKQAVRK ALIEERGRFC
210 220 230 240 250
TFISMLRPVI EEEISMLGEI THLQTISEDL KSLTMDPHKL PSSSEQVILD
260 270 280 290 300
LKGSDYSWSY QTPPSSPSTT MSRKSSVCSS LNSVNSSDSR SSGSHSHSPS
310 320 330 340 350
SHYRYRSSNL AQQAPVRLSS VSSHDSGFIS QDAFQSKSPS PMPPEAANQL
360 370 380 390 400
SNGFSHCSLS SESHAGPVGA GPFPHCLPAS RLLPRVTSVH LPDYAHYYTI
410 420 430 440 450
GPGMFPSSQI PSWKDWAKPG PYDQPLVNTL QRRKEKREPD SNGGGPTTTG
460 470 480 490 500
GPPAGAEEAQ RPRSMTVSAA TRPGEEMAAC EELTLALSRG LQLDVQRSSR
510 520 530 540 550
DSLQCSSGYS TQTTTPCCSE DTIPSQVSDY DYFSVSGDQE AEQQEFDKSS
560 570 580 590 600
TIPRNSDISQ SYRRMFQAKR PASTAGLPTT LGPAMVTPGV ATIRRTPSTK
610 620 630 640 650
PSVRRGTIGA GPIPIKTPVI PVKTPTVPDL PGVLPSPPDG PEERGEHSPE
660 670 680 690 700
SPSAGEGPQG VSNIPSSLWS GQAPVNPPLP GPKPSIPEEH RQAIPESEAE
710 720 730 740 750
DQERDPPSAT VSPGPIPESD PADLSPRESP QGEDMLNAIR RGVKLKKTTT

NDRSAPRFS
Length:759
Mass (Da):82,408
Last modified:June 1, 2002 - v1
Checksum:i3E9008065FF78439
GO
Isoform 2Curated (identifier: Q8R1S4-2) [UniParc]FASTAAdd to basket
Also known as: Short1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     483-483: L → P
     487-487: L → P
     646-681: Missing.

Show »
Length:723
Mass (Da):78,829
Checksum:i52B227AA050AB392
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139N → K in BAC27008 (PubMed:16141072).Curated1
Sequence conflicti758F → L in BAC27008 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050527483L → P in isoform 2. 1 Publication1
Alternative sequenceiVSP_050528487L → P in isoform 2. 1 Publication1
Alternative sequenceiVSP_050529646 – 681Missing in isoform 2. 1 PublicationAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY214918 mRNA. Translation: AAO52743.1.
BC003483 mRNA. Translation: AAH03483.1.
BC024131 mRNA. Translation: AAH24131.1.
BC042632 mRNA. Translation: AAH42632.1.
AK030533 mRNA. Translation: BAC27008.1.
CCDSiCCDS27496.1. [Q8R1S4-1]
RefSeqiNP_659049.2. NM_144800.2.
UniGeneiMm.215481.
Mm.394414.

Genome annotation databases

GeneIDi211401.
KEGGimmu:211401.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY214918 mRNA. Translation: AAO52743.1.
BC003483 mRNA. Translation: AAH03483.1.
BC024131 mRNA. Translation: AAH24131.1.
BC042632 mRNA. Translation: AAH42632.1.
AK030533 mRNA. Translation: BAC27008.1.
CCDSiCCDS27496.1. [Q8R1S4-1]
RefSeqiNP_659049.2. NM_144800.2.
UniGeneiMm.215481.
Mm.394414.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D1LX-ray1.85A/B1-250[»]
ProteinModelPortaliQ8R1S4.
SMRiQ8R1S4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29271N.
STRINGi10090.ENSMUSP00000079239.

PTM databases

iPTMnetiQ8R1S4.
PhosphoSitePlusiQ8R1S4.

Proteomic databases

PaxDbiQ8R1S4.
PRIDEiQ8R1S4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi211401.
KEGGimmu:211401.

Organism-specific databases

CTDi9788.
MGIiMGI:2384818. Mtss1.

Phylogenomic databases

eggNOGiENOG410IGVU. Eukaryota.
ENOG410XRT4. LUCA.
HOGENOMiHOG000113691.
HOVERGENiHBG052530.
InParanoidiQ8R1S4.
KOiK20128.
PhylomeDBiQ8R1S4.

Miscellaneous databases

ChiTaRSiMtss1. mouse.
EvolutionaryTraceiQ8R1S4.
PROiQ8R1S4.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MTSS1.

Family and domain databases

InterProiIPR013606. I-BAR_dom.
IPR027682. MIM.
IPR030127. MIM/ABBA.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR15708. PTHR15708. 1 hit.
PTHR15708:SF10. PTHR15708:SF10. 1 hit.
PfamiPF08397. IMD. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
PROSITEiPS51338. IMD. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTSS1_MOUSE
AccessioniPrimary (citable) accession number: Q8R1S4
Secondary accession number(s): Q8BMM3, Q99LB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.