ID   IL34_MOUSE              Reviewed;         235 AA.
AC   Q8R1R4; B2ZC71; B2ZC72; Q9D8F2;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   24-JAN-2024, entry version 124.
DE   RecName: Full=Interleukin-34;
DE            Short=IL-34;
DE   Flags: Precursor;
GN   Name=Il34;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3).
RC   STRAIN=C57BL/6J;
RX   PubMed=18467591; DOI=10.1126/science.1154370;
RA   Lin H., Lee E., Hestir K., Leo C., Huang M., Bosch E., Halenbeck R., Wu G.,
RA   Zhou A., Behrens D., Hollenbaugh D., Linnemann T., Qin M., Wong J., Chu K.,
RA   Doberstein S.K., Williams L.T.;
RT   "Discovery of a cytokine and its receptor by functional screening of the
RT   extracellular proteome.";
RL   Science 320:807-811(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=C57BL/6J; TISSUE=Small intestine;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=FVB/N; TISSUE=Salivary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Cytokine that promotes the proliferation, survival and
CC       differentiation of monocytes and macrophages. Promotes the release of
CC       pro-inflammatory chemokines, and thereby plays an important role in
CC       innate immunity and in inflammatory processes. Plays an important role
CC       in the regulation of osteoclast proliferation and differentiation, and
CC       in the regulation of bone resorption. Signaling via CSF1R and its
CC       downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND
CC       MAPK3/ERK1 (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. Interacts with CSF1R (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q8R1R4-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8R1R4-2; Sequence=VSP_026632;
CC       Name=3;
CC         IsoId=Q8R1R4-3; Sequence=VSP_035080;
CC   -!- SIMILARITY: Belongs to the IL-34 family. {ECO:0000305}.
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DR   EMBL; EU636995; ACD13453.1; -; mRNA.
DR   EMBL; EU636996; ACD13454.1; -; mRNA.
DR   EMBL; AK008082; BAB25448.1; -; mRNA.
DR   EMBL; BC016254; AAH16254.1; -; mRNA.
DR   CCDS; CCDS52669.1; -. [Q8R1R4-1]
DR   CCDS; CCDS52670.1; -. [Q8R1R4-2]
DR   RefSeq; NP_001128572.1; NM_001135100.2. [Q8R1R4-1]
DR   RefSeq; NP_083922.1; NM_029646.3. [Q8R1R4-2]
DR   RefSeq; XP_006531548.1; XM_006531485.3. [Q8R1R4-3]
DR   PDB; 4EXN; X-ray; 2.70 A; A/B/E/F=21-194.
DR   PDB; 4EXP; X-ray; 2.80 A; A=21-194.
DR   PDBsum; 4EXN; -.
DR   PDBsum; 4EXP; -.
DR   AlphaFoldDB; Q8R1R4; -.
DR   SMR; Q8R1R4; -.
DR   BioGRID; 218168; 2.
DR   STRING; 10090.ENSMUSP00000076120; -.
DR   GlyCosmos; Q8R1R4; 1 site, No reported glycans.
DR   GlyGen; Q8R1R4; 1 site.
DR   PaxDb; 10090-ENSMUSP00000076120; -.
DR   ProteomicsDB; 269395; -. [Q8R1R4-1]
DR   ProteomicsDB; 269396; -. [Q8R1R4-2]
DR   ProteomicsDB; 269397; -. [Q8R1R4-3]
DR   ABCD; Q8R1R4; 1 sequenced antibody.
DR   Antibodypedia; 30010; 483 antibodies from 32 providers.
DR   Ensembl; ENSMUST00000076846.11; ENSMUSP00000076120.5; ENSMUSG00000031750.16. [Q8R1R4-1]
DR   Ensembl; ENSMUST00000150680.2; ENSMUSP00000114398.2; ENSMUSG00000031750.16. [Q8R1R4-2]
DR   GeneID; 76527; -.
DR   KEGG; mmu:76527; -.
DR   UCSC; uc009nkz.3; mouse. [Q8R1R4-2]
DR   UCSC; uc009nla.3; mouse. [Q8R1R4-1]
DR   UCSC; uc012gko.1; mouse. [Q8R1R4-3]
DR   AGR; MGI:1923777; -.
DR   CTD; 146433; -.
DR   MGI; MGI:1923777; Il34.
DR   VEuPathDB; HostDB:ENSMUSG00000031750; -.
DR   eggNOG; ENOG502S2ET; Eukaryota.
DR   GeneTree; ENSGT00390000000932; -.
DR   HOGENOM; CLU_102223_1_0_1; -.
DR   InParanoid; Q8R1R4; -.
DR   OMA; WQDCELP; -.
DR   OrthoDB; 5322823at2759; -.
DR   PhylomeDB; Q8R1R4; -.
DR   TreeFam; TF337386; -.
DR   Reactome; R-MMU-449836; Other interleukin signaling.
DR   BioGRID-ORCS; 76527; 2 hits in 76 CRISPR screens.
DR   PRO; PR:Q8R1R4; -.
DR   Proteomes; UP000000589; Chromosome 8.
DR   RNAct; Q8R1R4; Protein.
DR   Bgee; ENSMUSG00000031750; Expressed in superior frontal gyrus and 84 other cell types or tissues.
DR   GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR   GO; GO:0005157; F:macrophage colony-stimulating factor receptor binding; ISS:UniProtKB.
DR   GO; GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0061514; P:interleukin-34-mediated signaling pathway; ISO:MGI.
DR   GO; GO:0061518; P:microglial cell proliferation; IGI:ARUK-UCL.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; ISS:UniProtKB.
DR   GO; GO:0010628; P:positive regulation of gene expression; IDA:ARUK-UCL.
DR   GO; GO:0010759; P:positive regulation of macrophage chemotaxis; ISO:MGI.
DR   GO; GO:0045651; P:positive regulation of macrophage differentiation; ISO:MGI.
DR   GO; GO:0120041; P:positive regulation of macrophage proliferation; ISO:MGI.
DR   GO; GO:0045657; P:positive regulation of monocyte differentiation; ISO:MGI.
DR   GO; GO:0048714; P:positive regulation of oligodendrocyte differentiation; IDA:UniProtKB.
DR   GO; GO:0001934; P:positive regulation of protein phosphorylation; ISS:UniProtKB.
DR   Gene3D; 1.20.1250.80; Interleukin-34; 1.
DR   InterPro; IPR020415; IL-34.
DR   InterPro; IPR038328; IL-34_sf.
DR   PANTHER; PTHR28606; INTERLEUKIN-34; 1.
DR   PANTHER; PTHR28606:SF1; INTERLEUKIN-34; 1.
DR   Pfam; PF15036; IL34; 1.
DR   PRINTS; PR01938; INTRLEUKIN34.
DR   Genevisible; Q8R1R4; MM.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; Cytokine; Glycoprotein; Growth factor;
KW   Immunity; Inflammatory response; Innate immunity; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..235
FT                   /note="Interleukin-34"
FT                   /id="PRO_0000294349"
FT   CARBOHYD        100
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         81
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:18467591"
FT                   /id="VSP_035080"
FT   VAR_SEQ         135..235
FT                   /note="DVEIGPHVEAVLSLLSTPGLSLKLVRPKALLDNCFRVMELLYCSCCKQSPIL
FT                   KWQDCELPRLHPHSPGSLMQCTATNVYPLSRQTPTSLPGSPSSSHGSLP -> AVGVHL
FT                   PGHVLVTLLSQLPGLPSPWARSFDTSWELLMMKGCGDWPSRGSCVISSEYSRPKPEAGA
FT                   AQSLAGQLLPGHGTAVLFLL (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_026632"
FT   STRAND          31..33
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   HELIX           37..44
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   HELIX           47..54
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   TURN            55..57
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   STRAND          64..66
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   HELIX           68..70
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   HELIX           74..81
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   TURN            82..84
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   HELIX           87..106
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   HELIX           116..131
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   TURN            132..135
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   HELIX           140..150
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   STRAND          157..159
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   HELIX           161..180
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   STRAND          181..184
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   HELIX           186..188
FT                   /evidence="ECO:0007829|PDB:4EXN"
FT   STRAND          189..191
FT                   /evidence="ECO:0007829|PDB:4EXP"
SQ   SEQUENCE   235 AA;  26764 MW;  4D30E33C977FD54D CRC64;
     MPWGLAWLYC LGILLDVALG NENLEIWTLT QDKECDLTGY LRGKLQYKNR LQYMKHYFPI
     NYRIAVPYEG VLRVANITRL QKAHVSEREL RYLWVLVSLN ATESVMDVLL EGHPSWKYLQ
     EVQTLLENVQ RSLMDVEIGP HVEAVLSLLS TPGLSLKLVR PKALLDNCFR VMELLYCSCC
     KQSPILKWQD CELPRLHPHS PGSLMQCTAT NVYPLSRQTP TSLPGSPSSS HGSLP
//