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Protein

Interleukin-34

Gene

Il34

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1 (By similarity).By similarity

GO - Molecular functioni

  • macrophage colony-stimulating factor receptor binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-34
Short name:
IL-34
Gene namesi
Name:Il34
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1923777. Il34.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 235215Interleukin-34PRO_0000294349Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ8R1R4.

Expressioni

Gene expression databases

BgeeiQ8R1R4.
GenevisibleiQ8R1R4. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with CSF1R (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076120.

Structurei

Secondary structure

1
235
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi31 – 333Combined sources
Helixi37 – 448Combined sources
Helixi47 – 548Combined sources
Turni55 – 573Combined sources
Beta strandi64 – 663Combined sources
Helixi68 – 703Combined sources
Helixi74 – 818Combined sources
Turni82 – 843Combined sources
Helixi87 – 10620Combined sources
Helixi116 – 13116Combined sources
Turni132 – 1354Combined sources
Helixi140 – 15011Combined sources
Beta strandi157 – 1593Combined sources
Helixi161 – 18020Combined sources
Beta strandi181 – 1844Combined sources
Helixi186 – 1883Combined sources
Beta strandi189 – 1913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EXNX-ray2.70A/B/E/F21-194[»]
4EXPX-ray2.80A21-194[»]
ProteinModelPortaliQ8R1R4.
SMRiQ8R1R4. Positions 23-194.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-34 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG46280.
GeneTreeiENSGT00390000000932.
HOGENOMiHOG000113048.
HOVERGENiHBG108003.
InParanoidiQ8R1R4.
OMAiYKISVPY.
OrthoDBiEOG7H7936.
PhylomeDBiQ8R1R4.
TreeFamiTF337386.

Family and domain databases

InterProiIPR020415. IL-34.
[Graphical view]
PfamiPF15036. IL34. 1 hit.
[Graphical view]
PRINTSiPR01938. INTRLEUKIN34.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R1R4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPWGLAWLYC LGILLDVALG NENLEIWTLT QDKECDLTGY LRGKLQYKNR
60 70 80 90 100
LQYMKHYFPI NYRIAVPYEG VLRVANITRL QKAHVSEREL RYLWVLVSLN
110 120 130 140 150
ATESVMDVLL EGHPSWKYLQ EVQTLLENVQ RSLMDVEIGP HVEAVLSLLS
160 170 180 190 200
TPGLSLKLVR PKALLDNCFR VMELLYCSCC KQSPILKWQD CELPRLHPHS
210 220 230
PGSLMQCTAT NVYPLSRQTP TSLPGSPSSS HGSLP
Length:235
Mass (Da):26,764
Last modified:June 1, 2002 - v1
Checksum:i4D30E33C977FD54D
GO
Isoform 2 (identifier: Q8R1R4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     135-235: DVEIGPHVEA...SPSSSHGSLP → AVGVHLPGHV...GHGTAVLFLL

Note: No experimental confirmation available.
Show »
Length:219
Mass (Da):24,736
Checksum:i55AD04CEB406C73C
GO
Isoform 3 (identifier: Q8R1R4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     81-81: Missing.

Show »
Length:234
Mass (Da):26,636
Checksum:i33A081F6099F1C12
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei81 – 811Missing in isoform 3. 1 PublicationVSP_035080
Alternative sequencei135 – 235101DVEIG…HGSLP → AVGVHLPGHVLVTLLSQLPG LPSPWARSFDTSWELLMMKG CGDWPSRGSCVISSEYSRPK PEAGAAQSLAGQLLPGHGTA VLFLL in isoform 2. 1 PublicationVSP_026632Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU636995 mRNA. Translation: ACD13453.1.
EU636996 mRNA. Translation: ACD13454.1.
AK008082 mRNA. Translation: BAB25448.1.
BC016254 mRNA. Translation: AAH16254.1.
CCDSiCCDS52669.1. [Q8R1R4-1]
CCDS52670.1. [Q8R1R4-2]
RefSeqiNP_001128572.1. NM_001135100.2. [Q8R1R4-1]
NP_083922.1. NM_029646.3. [Q8R1R4-2]
XP_006531548.1. XM_006531485.2. [Q8R1R4-3]
UniGeneiMm.77697.

Genome annotation databases

EnsembliENSMUST00000076846; ENSMUSP00000076120; ENSMUSG00000031750. [Q8R1R4-1]
ENSMUST00000150680; ENSMUSP00000114398; ENSMUSG00000031750. [Q8R1R4-2]
GeneIDi76527.
KEGGimmu:76527.
UCSCiuc009nkz.2. mouse. [Q8R1R4-2]
uc009nla.2. mouse. [Q8R1R4-1]
uc012gko.1. mouse. [Q8R1R4-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU636995 mRNA. Translation: ACD13453.1.
EU636996 mRNA. Translation: ACD13454.1.
AK008082 mRNA. Translation: BAB25448.1.
BC016254 mRNA. Translation: AAH16254.1.
CCDSiCCDS52669.1. [Q8R1R4-1]
CCDS52670.1. [Q8R1R4-2]
RefSeqiNP_001128572.1. NM_001135100.2. [Q8R1R4-1]
NP_083922.1. NM_029646.3. [Q8R1R4-2]
XP_006531548.1. XM_006531485.2. [Q8R1R4-3]
UniGeneiMm.77697.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EXNX-ray2.70A/B/E/F21-194[»]
4EXPX-ray2.80A21-194[»]
ProteinModelPortaliQ8R1R4.
SMRiQ8R1R4. Positions 23-194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076120.

Proteomic databases

PRIDEiQ8R1R4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076846; ENSMUSP00000076120; ENSMUSG00000031750. [Q8R1R4-1]
ENSMUST00000150680; ENSMUSP00000114398; ENSMUSG00000031750. [Q8R1R4-2]
GeneIDi76527.
KEGGimmu:76527.
UCSCiuc009nkz.2. mouse. [Q8R1R4-2]
uc009nla.2. mouse. [Q8R1R4-1]
uc012gko.1. mouse. [Q8R1R4-3]

Organism-specific databases

CTDi146433.
MGIiMGI:1923777. Il34.

Phylogenomic databases

eggNOGiNOG46280.
GeneTreeiENSGT00390000000932.
HOGENOMiHOG000113048.
HOVERGENiHBG108003.
InParanoidiQ8R1R4.
OMAiYKISVPY.
OrthoDBiEOG7H7936.
PhylomeDBiQ8R1R4.
TreeFamiTF337386.

Miscellaneous databases

NextBioi345322.
PROiQ8R1R4.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R1R4.
GenevisibleiQ8R1R4. MM.

Family and domain databases

InterProiIPR020415. IL-34.
[Graphical view]
PfamiPF15036. IL34. 1 hit.
[Graphical view]
PRINTSiPR01938. INTRLEUKIN34.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Discovery of a cytokine and its receptor by functional screening of the extracellular proteome."
    Lin H., Lee E., Hestir K., Leo C., Huang M., Bosch E., Halenbeck R., Wu G., Zhou A., Behrens D., Hollenbaugh D., Linnemann T., Qin M., Wong J., Chu K., Doberstein S.K., Williams L.T.
    Science 320:807-811(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Small intestine.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Salivary gland.

Entry informationi

Entry nameiIL34_MOUSE
AccessioniPrimary (citable) accession number: Q8R1R4
Secondary accession number(s): B2ZC71, B2ZC72, Q9D8F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: June 1, 2002
Last modified: June 24, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.