Q8R1N0 (ZN830_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 830

Alternative name(s):
Coiled-coil domain-containing protein 16
Ovus mutant candidate gene 1 protein

Gene names

Name:	Znf830
Synonyms:	Ccdc16, Omcg1, Zfp830

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as an important regulator of the cell cycle in the preimplantation embryo by controlling different aspects of M phase. Ref.5
Subcellular location	Nucleus. Chromosome. Note: Excluded from nucleolus. In metaphase II oocytes and in mitotic blastomeres, it is detected in cytoplasm, suggesting that it is not associated with chromosomes during mitosis. Ref.5
Tissue specificity	Widely expressed at low level. Ref.5
Developmental stage	Expressed in preimplantation embryos.
Disruption phenotype	Mice die by the end of preimplantation development and exhibit a dramatic reduction in the total cell number, a high mitotic index, and the presence of abnormal mitotic figures. Ref.5
Sequence similarities	Contains 1 C2H2-type zinc finger.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Chromosome Nucleus
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blastocyst growth Inferred from mutant phenotype Ref.5. Source: MGI cell cycle Inferred from mutant phenotype Ref.5. Source: MGI cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of S phase of mitotic cell cycle Inferred from mutant phenotype PubMed 21191184. Source: MGI
Cellular_component	chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: Compara nuclear speck Inferred from direct assay PubMed 21191184. Source: MGI
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Zinc finger protein 830		PRO_0000076194
Regions
	Zinc finger	53 – 75	23	C2H2-type
	Coiled coil	16 – 40	25	Potential
	Coiled coil	303 – 331	29	Potential
Amino acid modifications
	Modified residue	342	1	Phosphoserine Ref.4 Ref.6 Ref.7
	Modified residue	353	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	81 – 82	2	EL → DV in BAB30851. Ref.1
	Sequence conflict	111	1	Q → L in BAB26873. Ref.1
	Sequence conflict	305	1	C → G in BAE37178. Ref.1
	Sequence conflict	309	1	V → G in BAE37178. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8R1N0 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 1247580A0D8B6E60	FASTA	363	40,658
	10 20 30 40 50 60 MASSTSTRTP AGKRVVNQEE LRRLMREKQR LSTNRKRIES PFAKYNRLGQ LSCALCNTPV 70 80 90 100 110 120 KSELLWQTHV LGKQHRERVA ELKGAKGATQ GPSTGTVPQA TKRRATDVES QDAKKAKASA 130 140 150 160 170 180 GPQVQPSTSA SSANLDAARA APSKPGLGLL PDYDDEEEEE EEGGGEERRD SSKHLPDAQG 190 200 210 220 230 240 KEHSLASPRE TTSNVLPNDP FNTNPPKAPL VPHSGSIEKA EIHEKVVERR ENTAEALPEG 250 260 270 280 290 300 FFDDPEVDAK VRKVDAPKDQ MDKEWDEFQK AMRQVNTISE AIVAEEDEEG RLDRQIGEID 310 320 330 340 350 360 EQIECYRRVE KLRNRQDEIK NKLKEVLTIK ELQKKEEENV DSDDEGELQD LLSQDWRVKG ALL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Kidney, Liver, Skin and Thymus.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon.
[4]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-342, MASS SPECTROMETRY. Tissue: Embryonic brain.
[5]	"Impaired mitotic progression and preimplantation lethality in mice lacking OMCG1, a new evolutionarily conserved nuclear protein." Artus J., Vandormael-Pournin S., Froedin M., Nacerddine K., Babinet C., Cohen-Tannoudji M. Mol. Cell. Biol. 25:6289-6302(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-342 AND SER-353, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-342, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK050043 mRNA. Translation: BAC34045.1. AK010353 mRNA. Translation: BAB26873.1. AK017640 mRNA. Translation: BAB30851.1. AK132497 mRNA. Translation: BAE21204.1. AK148058 mRNA. Translation: BAE28318.1. AK163064 mRNA. Translation: BAE37178.1. AK167656 mRNA. Translation: BAE39707.1. AK168417 mRNA. Translation: BAE40331.1. AL645594 Genomic DNA. Translation: CAI25093.1. BC024340 mRNA. Translation: AAH24340.1.
IPI	IPI00320645.
RefSeq	NP_080160.2. NM_025884.4.
UniGene	Mm.29622.
3D structure databases
ProteinModelPortal	Q8R1N0.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4118608.
PTM databases
PhosphoSite	Q8R1N0.
Proteomic databases
PaxDb	Q8R1N0.
PRIDE	Q8R1N0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000056677; ENSMUSP00000056154; ENSMUSG00000046010.
GeneID	66983.
KEGG	mmu:66983.
UCSC	uc007kmy.1. mouse.
Organism-specific databases
CTD	66983.
MGI	MGI:1914233. Zfp830.
Phylogenomic databases
eggNOG	NOG306107.
GeneTree	ENSGT00390000012151.
HOGENOM	HOG000246999.
HOVERGEN	HBG105347.
InParanoid	Q8R1N0.
KO	K13104.
OMA	THVLGKQ.
OrthoDB	EOG4QFWFC.
Gene expression databases
Bgee	Q8R1N0.
CleanEx	MM_ZFP830.
Genevestigator	Q8R1N0.
GermOnline	ENSMUSG00000046010. Mus musculus.
Family and domain databases
InterPro	IPR025290. DUF4151. [Graphical view]
Pfam	PF13666. DUF4151. 1 hit. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	323216.
SOURCE	Search...

Entry information

Entry name

ZN830_MOUSE

Accession

Primary (citable) accession number: Q8R1N0
Secondary accession number(s): Q3TR52, Q9CWV9, Q9CYI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program