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Protein

5-phosphohydroxy-L-lysine phospho-lyase

Gene

Phykpl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the pyridoxal-phosphate-dependent breakdown of 5-phosphohydroxy-L-lysine, converting it to ammonia, inorganic phosphate and 2-aminoadipate semialdehyde.By similarity

Catalytic activityi

(5R)-5-phosphonooxy-L-lysine + H2O = (S)-2-amino-6-oxohexanoate + NH3 + phosphate.

Cofactori

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Lyase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-1442490. Collagen degradation.
R-MMU-71064. Lysine catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
5-phosphohydroxy-L-lysine phospho-lyase (EC:4.2.3.134)
Alternative name(s):
Alanine--glyoxylate aminotransferase 2-like 2
Gene namesi
Name:Phykpl
Synonyms:Agxt2l2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1920197. Phykpl.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4674675-phosphohydroxy-L-lysine phospho-lyasePRO_0000287668Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei278 – 2781N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

EPDiQ8R1K4.
MaxQBiQ8R1K4.
PaxDbiQ8R1K4.
PeptideAtlasiQ8R1K4.
PRIDEiQ8R1K4.

PTM databases

iPTMnetiQ8R1K4.
PhosphoSiteiQ8R1K4.

Expressioni

Gene expression databases

BgeeiQ8R1K4.
CleanExiMM_AGXT2L2.
ExpressionAtlasiQ8R1K4. baseline and differential.
GenevisibleiQ8R1K4. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000132190.

Structurei

3D structure databases

ProteinModelPortaliQ8R1K4.
SMRiQ8R1K4. Positions 30-436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1403. Eukaryota.
COG0160. LUCA.
GeneTreeiENSGT00530000062907.
HOGENOMiHOG000020206.
HOVERGENiHBG004196.
InParanoidiQ8R1K4.
KOiK18202.
OMAiHPIACVA.
PhylomeDBiQ8R1K4.
TreeFamiTF320468.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R1K4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAADTRAKAV TLDLRRRLLS SSCRLFFPED PVKIIRGQGQ YLYDEQGREY
60 70 80 90 100
LDCINNVAHV GHCHPTVVQA AHEQNLVLNT NSRYLHDNIV DYAQRLSETL
110 120 130 140 150
PEQLSVFYFL NSGSEANDLA LRLARQYTGH QDVVVLDHAY HGHLSSLIDI
160 170 180 190 200
SPYKFRNLGG QKEWVHVAPL PDTYRGPYRE DHPNPAEAYA NEVKHVISSA
210 220 230 240 250
QQKGRKIAAF FAESLPSVSG QIIPPAGYFS QVAEHIHRAG GLFVADEIQV
260 270 280 290 300
GFGRIGKHFW AFQLEGEDFV PDIVTMGKSI GNGHPVACMA TTQAVSRAFE
310 320 330 340 350
ATGVEYFNTF GGNPVSCAVG LAVLDVLKTE QLQAHATNVG SFLLEHLTQQ
360 370 380 390 400
KAKHPIIGDV RGTGLFIGVD LIKDETLRTP ATEEAEYLVS RLKENYILLS
410 420 430 440 450
IDGPGKNILK FKPPMCFNVD NAQHVVAKLD DILTDMEEKV RSCETLRIKH
460
PPEDTHPTQI LLTRQQD
Length:467
Mass (Da):51,966
Last modified:June 1, 2002 - v1
Checksum:i85DE6A02090E6758
GO
Isoform 2 (identifier: Q8R1K4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     453-467: EDTHPTQILLTRQQD → GSTGPGCRAATGNRPS

Show »
Length:468
Mass (Da):51,660
Checksum:i5D89D137828A1614
GO
Isoform 3 (identifier: Q8R1K4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     168-184: APLPDTYRGPYREDHPN → VCIPTISNRSVSIPKPG
     185-467: Missing.

Show »
Length:184
Mass (Da):20,825
Checksum:iD8677E8C1238C66B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei168 – 18417APLPD…EDHPN → VCIPTISNRSVSIPKPG in isoform 3. 1 PublicationVSP_025586Add
BLAST
Alternative sequencei185 – 467283Missing in isoform 3. 1 PublicationVSP_025587Add
BLAST
Alternative sequencei453 – 46715EDTHP…TRQQD → GSTGPGCRAATGNRPS in isoform 2. 1 PublicationVSP_025588Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK085984 mRNA. Translation: BAC39584.1.
AK087703 mRNA. Translation: BAC39972.1.
AK133235 mRNA. Translation: BAE21570.1.
AK160557 mRNA. Translation: BAE35872.1.
AL645602, AL662843 Genomic DNA. Translation: CAM26934.1.
AL662843, AL645602 Genomic DNA. Translation: CAI35931.1.
AL662843 Genomic DNA. Translation: CAI35932.1.
BC024461 mRNA. Translation: AAH24461.1.
CCDSiCCDS24653.1. [Q8R1K4-1]
RefSeqiNP_082674.1. NM_028398.2. [Q8R1K4-1]
UniGeneiMm.256875.

Genome annotation databases

EnsembliENSMUST00000020625; ENSMUSP00000020625; ENSMUSG00000020359. [Q8R1K4-2]
ENSMUST00000167797; ENSMUSP00000132190; ENSMUSG00000020359. [Q8R1K4-1]
GeneIDi72947.
KEGGimmu:72947.
UCSCiuc007itw.1. mouse. [Q8R1K4-3]
uc007itx.1. mouse. [Q8R1K4-2]
uc007ity.1. mouse. [Q8R1K4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK085984 mRNA. Translation: BAC39584.1.
AK087703 mRNA. Translation: BAC39972.1.
AK133235 mRNA. Translation: BAE21570.1.
AK160557 mRNA. Translation: BAE35872.1.
AL645602, AL662843 Genomic DNA. Translation: CAM26934.1.
AL662843, AL645602 Genomic DNA. Translation: CAI35931.1.
AL662843 Genomic DNA. Translation: CAI35932.1.
BC024461 mRNA. Translation: AAH24461.1.
CCDSiCCDS24653.1. [Q8R1K4-1]
RefSeqiNP_082674.1. NM_028398.2. [Q8R1K4-1]
UniGeneiMm.256875.

3D structure databases

ProteinModelPortaliQ8R1K4.
SMRiQ8R1K4. Positions 30-436.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000132190.

PTM databases

iPTMnetiQ8R1K4.
PhosphoSiteiQ8R1K4.

Proteomic databases

EPDiQ8R1K4.
MaxQBiQ8R1K4.
PaxDbiQ8R1K4.
PeptideAtlasiQ8R1K4.
PRIDEiQ8R1K4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020625; ENSMUSP00000020625; ENSMUSG00000020359. [Q8R1K4-2]
ENSMUST00000167797; ENSMUSP00000132190; ENSMUSG00000020359. [Q8R1K4-1]
GeneIDi72947.
KEGGimmu:72947.
UCSCiuc007itw.1. mouse. [Q8R1K4-3]
uc007itx.1. mouse. [Q8R1K4-2]
uc007ity.1. mouse. [Q8R1K4-1]

Organism-specific databases

CTDi85007.
MGIiMGI:1920197. Phykpl.

Phylogenomic databases

eggNOGiKOG1403. Eukaryota.
COG0160. LUCA.
GeneTreeiENSGT00530000062907.
HOGENOMiHOG000020206.
HOVERGENiHBG004196.
InParanoidiQ8R1K4.
KOiK18202.
OMAiHPIACVA.
PhylomeDBiQ8R1K4.
TreeFamiTF320468.

Enzyme and pathway databases

ReactomeiR-MMU-1442490. Collagen degradation.
R-MMU-71064. Lysine catabolism.

Miscellaneous databases

PROiQ8R1K4.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R1K4.
CleanExiMM_AGXT2L2.
ExpressionAtlasiQ8R1K4. baseline and differential.
GenevisibleiQ8R1K4. MM.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J.
    Tissue: Heart, Ovary and Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Kidney.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart and Lung.

Entry informationi

Entry nameiAT2L2_MOUSE
AccessioniPrimary (citable) accession number: Q8R1K4
Secondary accession number(s): Q3TUU9, Q8BG04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2002
Last modified: July 6, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.