Q8R1H0 (HOP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeodomain-only protein

Alternative name(s):
Homeobox-only protein
Odd homeobox protein 1
Short name=mOB1

Gene names

Name:	Hopx
Synonyms:	Hod, Hop, Ob1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	73 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy. Ref.1 Ref.2
Subunit structure	Interacts with serum response factor (SRF). Component of a large complex containing histone deacetylases such as HDAC2. Ref.1 Ref.2 Ref.6
Subcellular location	Nucleus. Cytoplasm. Note: According to Ref.3 it is cytoplasmic. Ref.1 Ref.2 Ref.3
Tissue specificity	Expressed in the embryonic and adult heart and in the adult brain, liver, lung, skeletal muscle, intestine and spleen. Throughout embryonic and postnatal development, it is expressed in the myocardium. Ref.1 Ref.2 Ref.3
Developmental stage	First detected at E7.75 in trophoblasts within extraembryonic membranes, in the lateral wings of the cardiac crescent and in the anterior head folds. At E8.0, it is expressed along the length of the linear heart tube and in the head folds. Expressed throughout the myocardium at E9.5 and in the branchial arches. At E12.5, it is expressed in the heart and in the ventricular zone of the neural tube. At E13.5, it is weakly expressed in the intestinal epithelium. At E13.5 and E15.5, it is also expressed in skeletal muscle, stratified epithelium (upper aerodigestive tract and skin), epithelium of developing airways, vibrissae, midbrain/hindbrain junction, meninges, mesenchymal cellular condensations that preceded cartilage formation and chondrocytes. Ref.1 Ref.2 Ref.3
Induction	By the transcription factor NKX2-5 that acts as a direct regulator. Ref.1
Disruption phenotype	Mice display partial embryonic lethality and heart failure. Ref.1 Ref.2
Sequence similarities	Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Homeobox
Molecular function	Developmental protein Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	heart development Inferred from mutant phenotype Ref.1Ref.2. Source: MGI histone deacetylation Inferred from genetic interaction Ref.6. Source: MGI lung alveolus development Inferred from mutant phenotype. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.1. Source: MGI positive regulation of skeletal muscle tissue regeneration Inferred from mutant phenotype. Source: MGI positive regulation of striated muscle cell differentiation Inferred from mutant phenotype. Source: MGI regulation of heart contraction Inferred from mutant phenotype. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW trophectodermal cell differentiation Inferred from mutant phenotype. Source: MGI
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.1. Source: MGI
Molecular function	protein binding Inferred from physical interaction Ref.2Ref.6. Source: MGI sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 73

73

Homeodomain-only protein

PRO_0000049130

Regions

DNA binding

3 – 62

60

Homeobox; atypical

Experimental info

Mutagenesis

35 – 39

5

LIAAE → AAASM in H2; induces a strong reduction in interaction with SRF and subsequent ability to prevent transcription of cardiac-specific genes. Ref.1

Mutagenesis

49 – 53

5

KWFKQ → AAASM in H3; does not affect the interaction with SRF and keeps its ability to prevent transcription of cardiac-specific genes. Ref.1

Sequence conflict

6

1

A → P in BAC25235. Ref.4

Sequence conflict

6

1

A → V in AAN16320. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

73

Helix Strand Turn

Details...

Helix

11 – 22

12

Helix

30 – 40

11

Helix

44 – 62

19

Sequences

	Sequence		Length	Mass (Da)
	Q8R1H0 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 9D9B6D7F7A11C592	FASTA	73	8,282
	10 20 30 40 50 60 MSAQTASGPT EDQVEILEYN FNKVNKHPDP TTLCLIAAEA GLTEEQTQKW FKQRLAEWRR 70 SEGLPSECRS VTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Hop is an unusual homeobox gene that modulates cardiac development." Chen F., Kook H., Milewski R., Gitler A.D., Lu M.M., Li J., Nazarian R., Schnepp R., Jen K., Biben C., Runke G., Mackay J.P., Novotny J., Schwartz R.J., Harvey R.P., Mullins M.C., Epstein J.A. Cell 110:713-723(2002) [PubMed: 12297045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, INTERACTION WITH SRF, MUTAGENESIS OF 35-LEU--GLU-39 AND 49-LYS--GLN-53, DISRUPTION PHENOTYPE. Strain: NIH Swiss.
[2]	"Modulation of cardiac growth and development by HOP, an unusual homeodomain protein." Shin C.H., Liu Z.-P., Passier R., Zhang C.-L., Wang D.-Z., Harris T.M., Yamagishi H., Richardson J.A., Childs G., Olson E.N. Cell 110:725-735(2002) [PubMed: 12297046] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH SRF, DISRUPTION PHENOTYPE. Strain: C57BL/6J. Tissue: Heart.
[3]	"Expression of mOb1, a novel atypical 73 amino acid K50-homeodomain protein, during mouse development." Adu J., Leong F.T., Smith N.R., Leek J.P., Markham A.F., Robinson P.A., Mighell A.J. Mech. Dev. 119:S43-S47(2002) [PubMed: 14516659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: CD-1.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung, Small intestine and Tongue.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon.
[6]	"Cardiac hypertrophy and histone deacetylase-dependent transcriptional repression mediated by the atypical homeodomain protein Hop." Kook H., Lepore J.J., Gitler A.D., Lu M.M., Yung W.W.-M., Mackay J., Zhou R., Ferrari V., Gruber P., Epstein J.A. J. Clin. Invest. 112:863-871(2003) [PubMed: 12975471] [Abstract] Cited for: INTERACTION WITH HDAC2.
[7]	"Solution structure of mouse homeodomain-only protein Hop." RIKEN structural genomics initiative (RSGI) Submitted (JUL-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 10-73.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF536202 mRNA. Translation: AAN16320.1.
AF534182 mRNA. Translation: AAN05634.1.
AF492703 mRNA. Translation: AAM46823.1.
AF492704 mRNA. Translation: AAM46824.1.
AK003784 mRNA. Translation: BAC25054.1.
AK004798 mRNA. Translation: BAC25097.1.
AK008297 mRNA. Translation: BAC25214.1.
AK008439 mRNA. Translation: BAC25219.1.
AK009007 mRNA. Translation: BAC25235.1.
BC024546 mRNA. Translation: AAH24546.1.

IPI

IPI00153376.

RefSeq

NP_001153372.1. NM_001159900.1.
NP_001153373.1. NM_001159901.1.
NP_783199.1. NM_175606.3.

UniGene

Mm.181852.
Mm.478741.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UHS	NMR	-	A	10-73	[»]
2HI3	NMR	-	A	1-73	[»]

ProteinModelPortal

Q8R1H0.

SMR

Q8R1H0. Positions 1-73.

ModBase

Search...

Protein-protein interaction databases

STRING

Q8R1H0.

Proteomic databases

PRIDE

Q8R1H0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000081964; ENSMUSP00000080630; ENSMUSG00000059325.
ENSMUST00000113453; ENSMUSP00000109080; ENSMUSG00000059325.
ENSMUST00000120827; ENSMUSP00000113295; ENSMUSG00000059325.

GeneID

74318.

KEGG

mmu:74318.

UCSC

uc008xvv.2. mouse.

Organism-specific databases

CTD

84525.

MGI

MGI:1916782. Hopx.

Phylogenomic databases

eggNOG

roNOG17957.

GeneTree

ENSGT00390000017143.

HOGENOM

HBG714841.

HOVERGEN

HBG051921.

InParanoid

Q8R1H0.

OMA

EEETQKW.

OrthoDB

EOG4M39B5.

PhylomeDB

Q8R1H0.

Gene expression databases

ArrayExpress

Q8R1H0.

Bgee

Q8R1H0.

CleanEx

MM_HOPX.

Genevestigator

Q8R1H0.

GermOnline

ENSMUSG00000059325. Mus musculus.

Family and domain databases

InterPro

IPR001356. Homeobox.
IPR009057. Homeodomain-like.
IPR012287. Homeodomain-rel.
[Graphical view]

Gene3D

G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.

Pfam

PF00046. Homeobox. 1 hit.
[Graphical view]

SMART

SM00389. HOX. 1 hit.
[Graphical view]

SUPFAM

SSF46689. Homeodomain_like. 1 hit.

PROSITE

PS00027. HOMEOBOX_1. False negative.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

340437.

SOURCE

Search...

Entry information

Entry name

HOP_MOUSE

Accession

Primary (citable) accession number: Q8R1H0
Secondary accession number(s): Q8C1N5, Q8CJ22

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program