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Q8R1H0

- HOP_MOUSE

UniProt

Q8R1H0 - HOP_MOUSE

Protein

Homeodomain-only protein

Gene

Hopx

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi3 – 6260Homeobox; atypicalPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. sequence-specific DNA binding Source: InterPro
    3. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. heart development Source: MGI
    2. histone deacetylation Source: MGI
    3. lung alveolus development Source: MGI
    4. negative regulation of cell differentiation Source: Ensembl
    5. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    6. positive regulation of skeletal muscle tissue regeneration Source: MGI
    7. positive regulation of striated muscle cell differentiation Source: MGI
    8. regulation of heart contraction Source: MGI
    9. regulation of protein binding Source: MGI
    10. transcription, DNA-templated Source: UniProtKB-KW
    11. trophectodermal cell differentiation Source: MGI

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homeodomain-only protein
    Alternative name(s):
    Homeobox-only protein
    Odd homeobox protein 1
    Short name:
    mOB1
    Gene namesi
    Name:Hopx
    Synonyms:Hod, Hop, Ob1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1916782. Hopx.

    Subcellular locationi

    Nucleus. Cytoplasm
    Note: According to PubMed:14516659 it is cytoplasmic.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice display partial embryonic lethality and heart failure.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi35 – 395LIAAE → AAASM in H2; induces a strong reduction in interaction with SRF and subsequent ability to prevent transcription of cardiac-specific genes. 1 Publication
    Mutagenesisi49 – 535KWFKQ → AAASM in H3; does not affect the interaction with SRF and keeps its ability to prevent transcription of cardiac-specific genes. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7373Homeodomain-only proteinPRO_0000049130Add
    BLAST

    Proteomic databases

    MaxQBiQ8R1H0.
    PaxDbiQ8R1H0.
    PRIDEiQ8R1H0.

    Expressioni

    Tissue specificityi

    Expressed in the embryonic and adult heart and in the adult brain, liver, lung, skeletal muscle, intestine and spleen. Throughout embryonic and postnatal development, it is expressed in the myocardium.3 Publications

    Developmental stagei

    First detected at E7.75 in trophoblasts within extraembryonic membranes, in the lateral wings of the cardiac crescent and in the anterior head folds. At E8.0, it is expressed along the length of the linear heart tube and in the head folds. Expressed throughout the myocardium at E9.5 and in the branchial arches. At E12.5, it is expressed in the heart and in the ventricular zone of the neural tube. At E13.5, it is weakly expressed in the intestinal epithelium. At E13.5 and E15.5, it is also expressed in skeletal muscle, stratified epithelium (upper aerodigestive tract and skin), epithelium of developing airways, vibrissae, midbrain/hindbrain junction, meninges, mesenchymal cellular condensations that preceded cartilage formation and chondrocytes.3 Publications

    Inductioni

    By the transcription factor NKX2-5 that acts as a direct regulator.1 Publication

    Gene expression databases

    BgeeiQ8R1H0.
    CleanExiMM_HOPX.
    GenevestigatoriQ8R1H0.

    Interactioni

    Subunit structurei

    Interacts with serum response factor (SRF). Component of a large complex containing histone deacetylases such as HDAC2.3 Publications

    Protein-protein interaction databases

    BioGridi216662. 2 interactions.
    IntActiQ8R1H0. 1 interaction.
    MINTiMINT-4097829.

    Structurei

    Secondary structure

    1
    73
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi11 – 2212
    Helixi30 – 4011
    Helixi44 – 6219
    Beta strandi66 – 683

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1UHSNMR-A10-73[»]
    2HI3NMR-A1-73[»]
    ProteinModelPortaliQ8R1H0.
    SMRiQ8R1H0. Positions 1-73.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8R1H0.

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG319963.
    GeneTreeiENSGT00390000017143.
    HOGENOMiHOG000112932.
    HOVERGENiHBG051921.
    InParanoidiQ8R1H0.
    OMAiFKLRNAQ.
    OrthoDBiEOG780RQF.
    PhylomeDBiQ8R1H0.
    TreeFamiTF330730.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    [Graphical view]
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8R1H0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAQTASGPT EDQVEILEYN FNKVNKHPDP TTLCLIAAEA GLTEEQTQKW   50
    FKQRLAEWRR SEGLPSECRS VTD 73
    Length:73
    Mass (Da):8,282
    Last modified:June 1, 2002 - v1
    Checksum:i9D9B6D7F7A11C592
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61A → P in BAC25235. (PubMed:16141072)Curated
    Sequence conflicti6 – 61A → V in AAN16320. (PubMed:12297045)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF536202 mRNA. Translation: AAN16320.1.
    AF534182 mRNA. Translation: AAN05634.1.
    AF492703 mRNA. Translation: AAM46823.1.
    AF492704 mRNA. Translation: AAM46824.1.
    AK003784 mRNA. Translation: BAC25054.1.
    AK004798 mRNA. Translation: BAC25097.1.
    AK008297 mRNA. Translation: BAC25214.1.
    AK008439 mRNA. Translation: BAC25219.1.
    AK009007 mRNA. Translation: BAC25235.1.
    BC024546 mRNA. Translation: AAH24546.1.
    CCDSiCCDS39120.1.
    RefSeqiNP_001153372.1. NM_001159900.1.
    NP_001153373.1. NM_001159901.1.
    NP_783199.1. NM_175606.3.
    UniGeneiMm.181852.
    Mm.478741.

    Genome annotation databases

    EnsembliENSMUST00000081964; ENSMUSP00000080630; ENSMUSG00000059325.
    ENSMUST00000113453; ENSMUSP00000109080; ENSMUSG00000059325.
    ENSMUST00000120827; ENSMUSP00000113295; ENSMUSG00000059325.
    GeneIDi74318.
    KEGGimmu:74318.
    UCSCiuc008xvv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF536202 mRNA. Translation: AAN16320.1 .
    AF534182 mRNA. Translation: AAN05634.1 .
    AF492703 mRNA. Translation: AAM46823.1 .
    AF492704 mRNA. Translation: AAM46824.1 .
    AK003784 mRNA. Translation: BAC25054.1 .
    AK004798 mRNA. Translation: BAC25097.1 .
    AK008297 mRNA. Translation: BAC25214.1 .
    AK008439 mRNA. Translation: BAC25219.1 .
    AK009007 mRNA. Translation: BAC25235.1 .
    BC024546 mRNA. Translation: AAH24546.1 .
    CCDSi CCDS39120.1.
    RefSeqi NP_001153372.1. NM_001159900.1.
    NP_001153373.1. NM_001159901.1.
    NP_783199.1. NM_175606.3.
    UniGenei Mm.181852.
    Mm.478741.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1UHS NMR - A 10-73 [» ]
    2HI3 NMR - A 1-73 [» ]
    ProteinModelPortali Q8R1H0.
    SMRi Q8R1H0. Positions 1-73.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 216662. 2 interactions.
    IntActi Q8R1H0. 1 interaction.
    MINTi MINT-4097829.

    Proteomic databases

    MaxQBi Q8R1H0.
    PaxDbi Q8R1H0.
    PRIDEi Q8R1H0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000081964 ; ENSMUSP00000080630 ; ENSMUSG00000059325 .
    ENSMUST00000113453 ; ENSMUSP00000109080 ; ENSMUSG00000059325 .
    ENSMUST00000120827 ; ENSMUSP00000113295 ; ENSMUSG00000059325 .
    GeneIDi 74318.
    KEGGi mmu:74318.
    UCSCi uc008xvv.2. mouse.

    Organism-specific databases

    CTDi 84525.
    MGIi MGI:1916782. Hopx.

    Phylogenomic databases

    eggNOGi NOG319963.
    GeneTreei ENSGT00390000017143.
    HOGENOMi HOG000112932.
    HOVERGENi HBG051921.
    InParanoidi Q8R1H0.
    OMAi FKLRNAQ.
    OrthoDBi EOG780RQF.
    PhylomeDBi Q8R1H0.
    TreeFami TF330730.

    Miscellaneous databases

    EvolutionaryTracei Q8R1H0.
    NextBioi 340437.
    PROi Q8R1H0.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8R1H0.
    CleanExi MM_HOPX.
    Genevestigatori Q8R1H0.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    [Graphical view ]
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, INTERACTION WITH SRF, MUTAGENESIS OF 35-LEU--GLU-39 AND 49-LYS--GLN-53, DISRUPTION PHENOTYPE.
      Strain: NIH Swiss.
    2. "Modulation of cardiac growth and development by HOP, an unusual homeodomain protein."
      Shin C.H., Liu Z.-P., Passier R., Zhang C.-L., Wang D.-Z., Harris T.M., Yamagishi H., Richardson J.A., Childs G., Olson E.N.
      Cell 110:725-735(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH SRF, DISRUPTION PHENOTYPE.
      Strain: C57BL/6J.
      Tissue: Heart.
    3. "Expression of mOb1, a novel atypical 73 amino acid K50-homeodomain protein, during mouse development."
      Adu J., Leong F.T., Smith N.R., Leek J.P., Markham A.F., Robinson P.A., Mighell A.J.
      Mech. Dev. 119:S43-S47(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: CD-1.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Lung, Small intestine and Tongue.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon.
    6. "Cardiac hypertrophy and histone deacetylase-dependent transcriptional repression mediated by the atypical homeodomain protein Hop."
      Kook H., Lepore J.J., Gitler A.D., Lu M.M., Yung W.W.-M., Mackay J., Zhou R., Ferrari V., Gruber P., Epstein J.A.
      J. Clin. Invest. 112:863-871(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HDAC2.
    7. "Solution structure of mouse homeodomain-only protein Hop."
      RIKEN structural genomics initiative (RSGI)
      Submitted (JUL-2004) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 10-73.

    Entry informationi

    Entry nameiHOP_MOUSE
    AccessioniPrimary (citable) accession number: Q8R1H0
    Secondary accession number(s): Q8C1N5, Q8CJ22
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3