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Q8R1H0

- HOP_MOUSE

UniProt

Q8R1H0 - HOP_MOUSE

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Protein

Homeodomain-only protein

Gene
Hopx, Hod, Hop, Ob1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi3 – 6260Homeobox; atypicalAdd
BLAST

GO - Molecular functioni

  1. protein binding Source: MGI
  2. sequence-specific DNA binding Source: InterPro
  3. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. heart development Source: MGI
  2. histone deacetylation Source: MGI
  3. lung alveolus development Source: MGI
  4. negative regulation of cell differentiation Source: Ensembl
  5. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  6. positive regulation of skeletal muscle tissue regeneration Source: MGI
  7. positive regulation of striated muscle cell differentiation Source: MGI
  8. regulation of heart contraction Source: MGI
  9. regulation of protein binding Source: MGI
  10. transcription, DNA-templated Source: UniProtKB-KW
  11. trophectodermal cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Homeodomain-only protein
Alternative name(s):
Homeobox-only protein
Odd homeobox protein 1
Short name:
mOB1
Gene namesi
Name:Hopx
Synonyms:Hod, Hop, Ob1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1916782. Hopx.

Subcellular locationi

Nucleus. Cytoplasm
Note: According to 1 Publication it is cytoplasmic.3 Publications

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice display partial embryonic lethality and heart failure.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi35 – 395LIAAE → AAASM in H2; induces a strong reduction in interaction with SRF and subsequent ability to prevent transcription of cardiac-specific genes. 1 Publication
Mutagenesisi49 – 535KWFKQ → AAASM in H3; does not affect the interaction with SRF and keeps its ability to prevent transcription of cardiac-specific genes. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7373Homeodomain-only proteinPRO_0000049130Add
BLAST

Proteomic databases

MaxQBiQ8R1H0.
PaxDbiQ8R1H0.
PRIDEiQ8R1H0.

Expressioni

Tissue specificityi

Expressed in the embryonic and adult heart and in the adult brain, liver, lung, skeletal muscle, intestine and spleen. Throughout embryonic and postnatal development, it is expressed in the myocardium.3 Publications

Developmental stagei

First detected at E7.75 in trophoblasts within extraembryonic membranes, in the lateral wings of the cardiac crescent and in the anterior head folds. At E8.0, it is expressed along the length of the linear heart tube and in the head folds. Expressed throughout the myocardium at E9.5 and in the branchial arches. At E12.5, it is expressed in the heart and in the ventricular zone of the neural tube. At E13.5, it is weakly expressed in the intestinal epithelium. At E13.5 and E15.5, it is also expressed in skeletal muscle, stratified epithelium (upper aerodigestive tract and skin), epithelium of developing airways, vibrissae, midbrain/hindbrain junction, meninges, mesenchymal cellular condensations that preceded cartilage formation and chondrocytes.3 Publications

Inductioni

By the transcription factor NKX2-5 that acts as a direct regulator.1 Publication

Gene expression databases

BgeeiQ8R1H0.
CleanExiMM_HOPX.
GenevestigatoriQ8R1H0.

Interactioni

Subunit structurei

Interacts with serum response factor (SRF). Component of a large complex containing histone deacetylases such as HDAC2.3 Publications

Protein-protein interaction databases

BioGridi216662. 2 interactions.
IntActiQ8R1H0. 1 interaction.
MINTiMINT-4097829.

Structurei

Secondary structure

1
73
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 2212
Helixi30 – 4011
Helixi44 – 6219
Beta strandi66 – 683

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UHSNMR-A10-73[»]
2HI3NMR-A1-73[»]
ProteinModelPortaliQ8R1H0.
SMRiQ8R1H0. Positions 1-73.

Miscellaneous databases

EvolutionaryTraceiQ8R1H0.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG319963.
GeneTreeiENSGT00390000017143.
HOGENOMiHOG000112932.
HOVERGENiHBG051921.
InParanoidiQ8R1H0.
OMAiFKLRNAQ.
OrthoDBiEOG780RQF.
PhylomeDBiQ8R1H0.
TreeFamiTF330730.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R1H0-1 [UniParc]FASTAAdd to Basket

« Hide

MSAQTASGPT EDQVEILEYN FNKVNKHPDP TTLCLIAAEA GLTEEQTQKW   50
FKQRLAEWRR SEGLPSECRS VTD 73
Length:73
Mass (Da):8,282
Last modified:June 1, 2002 - v1
Checksum:i9D9B6D7F7A11C592
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61A → P in BAC25235. 1 Publication
Sequence conflicti6 – 61A → V in AAN16320. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF536202 mRNA. Translation: AAN16320.1.
AF534182 mRNA. Translation: AAN05634.1.
AF492703 mRNA. Translation: AAM46823.1.
AF492704 mRNA. Translation: AAM46824.1.
AK003784 mRNA. Translation: BAC25054.1.
AK004798 mRNA. Translation: BAC25097.1.
AK008297 mRNA. Translation: BAC25214.1.
AK008439 mRNA. Translation: BAC25219.1.
AK009007 mRNA. Translation: BAC25235.1.
BC024546 mRNA. Translation: AAH24546.1.
CCDSiCCDS39120.1.
RefSeqiNP_001153372.1. NM_001159900.1.
NP_001153373.1. NM_001159901.1.
NP_783199.1. NM_175606.3.
UniGeneiMm.181852.
Mm.478741.

Genome annotation databases

EnsembliENSMUST00000081964; ENSMUSP00000080630; ENSMUSG00000059325.
ENSMUST00000113453; ENSMUSP00000109080; ENSMUSG00000059325.
ENSMUST00000120827; ENSMUSP00000113295; ENSMUSG00000059325.
GeneIDi74318.
KEGGimmu:74318.
UCSCiuc008xvv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF536202 mRNA. Translation: AAN16320.1 .
AF534182 mRNA. Translation: AAN05634.1 .
AF492703 mRNA. Translation: AAM46823.1 .
AF492704 mRNA. Translation: AAM46824.1 .
AK003784 mRNA. Translation: BAC25054.1 .
AK004798 mRNA. Translation: BAC25097.1 .
AK008297 mRNA. Translation: BAC25214.1 .
AK008439 mRNA. Translation: BAC25219.1 .
AK009007 mRNA. Translation: BAC25235.1 .
BC024546 mRNA. Translation: AAH24546.1 .
CCDSi CCDS39120.1.
RefSeqi NP_001153372.1. NM_001159900.1.
NP_001153373.1. NM_001159901.1.
NP_783199.1. NM_175606.3.
UniGenei Mm.181852.
Mm.478741.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1UHS NMR - A 10-73 [» ]
2HI3 NMR - A 1-73 [» ]
ProteinModelPortali Q8R1H0.
SMRi Q8R1H0. Positions 1-73.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 216662. 2 interactions.
IntActi Q8R1H0. 1 interaction.
MINTi MINT-4097829.

Proteomic databases

MaxQBi Q8R1H0.
PaxDbi Q8R1H0.
PRIDEi Q8R1H0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000081964 ; ENSMUSP00000080630 ; ENSMUSG00000059325 .
ENSMUST00000113453 ; ENSMUSP00000109080 ; ENSMUSG00000059325 .
ENSMUST00000120827 ; ENSMUSP00000113295 ; ENSMUSG00000059325 .
GeneIDi 74318.
KEGGi mmu:74318.
UCSCi uc008xvv.2. mouse.

Organism-specific databases

CTDi 84525.
MGIi MGI:1916782. Hopx.

Phylogenomic databases

eggNOGi NOG319963.
GeneTreei ENSGT00390000017143.
HOGENOMi HOG000112932.
HOVERGENi HBG051921.
InParanoidi Q8R1H0.
OMAi FKLRNAQ.
OrthoDBi EOG780RQF.
PhylomeDBi Q8R1H0.
TreeFami TF330730.

Miscellaneous databases

EvolutionaryTracei Q8R1H0.
NextBioi 340437.
PROi Q8R1H0.
SOURCEi Search...

Gene expression databases

Bgeei Q8R1H0.
CleanExi MM_HOPX.
Genevestigatori Q8R1H0.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
InterProi IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
[Graphical view ]
SMARTi SM00389. HOX. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS50071. HOMEOBOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, INTERACTION WITH SRF, MUTAGENESIS OF 35-LEU--GLU-39 AND 49-LYS--GLN-53, DISRUPTION PHENOTYPE.
    Strain: NIH Swiss.
  2. "Modulation of cardiac growth and development by HOP, an unusual homeodomain protein."
    Shin C.H., Liu Z.-P., Passier R., Zhang C.-L., Wang D.-Z., Harris T.M., Yamagishi H., Richardson J.A., Childs G., Olson E.N.
    Cell 110:725-735(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH SRF, DISRUPTION PHENOTYPE.
    Strain: C57BL/6J.
    Tissue: Heart.
  3. "Expression of mOb1, a novel atypical 73 amino acid K50-homeodomain protein, during mouse development."
    Adu J., Leong F.T., Smith N.R., Leek J.P., Markham A.F., Robinson P.A., Mighell A.J.
    Mech. Dev. 119:S43-S47(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: CD-1.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Lung, Small intestine and Tongue.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.
  6. "Cardiac hypertrophy and histone deacetylase-dependent transcriptional repression mediated by the atypical homeodomain protein Hop."
    Kook H., Lepore J.J., Gitler A.D., Lu M.M., Yung W.W.-M., Mackay J., Zhou R., Ferrari V., Gruber P., Epstein J.A.
    J. Clin. Invest. 112:863-871(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HDAC2.
  7. "Solution structure of mouse homeodomain-only protein Hop."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JUL-2004) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 10-73.

Entry informationi

Entry nameiHOP_MOUSE
AccessioniPrimary (citable) accession number: Q8R1H0
Secondary accession number(s): Q8C1N5, Q8CJ22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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