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Q8R1G6 (PDLI2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PDZ and LIM domain protein 2
Alternative name(s):
PDZ-LIM protein mystique
Gene names
Name:Pdlim2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity By similarity.

Subunit structure

Interacts with alpha-actinins ACTN1 and ACTN4, FLNA and MYH9 By similarity.

Subcellular location

Cytoplasm. Cytoplasmcytoskeleton. Note: Localizes at the cytoskeleton. Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin (PXN) By similarity.

Tissue specificity

Highly expressed in lung. Expressed at intermediate level in kidney, testis and spleen. Weakly expressed in heart and brain. Ref.2

Induction

Regulated by IGF-1. Ref.2

Sequence similarities

Contains 1 LIM zinc-binding domain.

Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
   Coding sequence diversityAlternative splicing
   DomainLIM domain
   LigandMetal-binding
Zinc
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentactin cytoskeleton

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

focal adhesion

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionzinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced.
Isoform 1 (identifier: Q8R1G6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349PDZ and LIM domain protein 2
PRO_0000075864

Regions

Domain1 – 8484PDZ
Domain281 – 34161LIM zinc-binding

Amino acid modifications

Modified residue1991Phosphoserine Ref.3
Modified residue2051Phosphoserine Ref.3

Secondary structure

................. 349
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 9809D7F1AF2BD907

FASTA34937,703
        10         20         30         40         50         60 
MALTVDVAGP APWGFRISGG RDFHTPIIVT KVTERGKAEA ADLRPGDIIV AINGQSAENM 

        70         80         90        100        110        120 
LHAEAQSKIR QSASPLRLQL DRSQTASPGQ TNGEGSLEVL ATRFQGSLRT HRDSQSSQRS 

       130        140        150        160        170        180 
ACFSPVSLSP RPCSPFSTPP PTSPVALSKE DMIGCSFQSL THSPGLAAAH HLTYPGHPTS 

       190        200        210        220        230        240 
QQAGHSSPSD SAVRVLLHSP GRPSSPRFSS LDLEEDSEVF KMLQENRQGR AAPRQSSSFR 

       250        260        270        280        290        300 
LLQEALEAEE RGGTPAFVPS SLSSQASLPT SRALATPPKL HTCEKCSVNI SNQAVRIQEG 

       310        320        330        340 
RYRHPGCYTC ADCGLNLKMR GHFWVGNELY CEKHARQRYS MPGTLNSRA 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Colon.
[2]"Mystique is a new IGF-I regulated PDZ-LIM domain protein that promotes cell attachment and migration and suppresses anchorage independent growth."
Loughran G., Healy N., Kiely P.A., Huigsloot M., Kedersha N.L., O'connor R.
Mol. Biol. Cell 16:1811-1822(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[3]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-205, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[4]"Solution structure of the PDZ domain of PDZ and LIM domain 2."
RIKEN structural genomics initiative (RSGI)
Submitted (FEB-2004) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 3-83.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC024556 mRNA. Translation: AAH24556.1.
CCDSCCDS27248.1. [Q8R1G6-1]
RefSeqNP_001240665.1. NM_001253736.1. [Q8R1G6-1]
NP_666090.1. NM_145978.2. [Q8R1G6-1]
UniGeneMm.283968.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1VB7NMR-A3-83[»]
ProteinModelPortalQ8R1G6.
SMRQ8R1G6. Positions 2-89, 279-344.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid229389. 2 interactions.
IntActQ8R1G6. 2 interactions.
MINTMINT-4118594.

PTM databases

PhosphoSiteQ8R1G6.

Proteomic databases

MaxQBQ8R1G6.
PaxDbQ8R1G6.
PRIDEQ8R1G6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022681; ENSMUSP00000022681; ENSMUSG00000022090. [Q8R1G6-1]
ENSMUST00000153735; ENSMUSP00000116200; ENSMUSG00000022090. [Q8R1G6-1]
GeneID213019.
KEGGmmu:213019.
UCSCuc007uni.1. mouse. [Q8R1G6-1]

Organism-specific databases

CTD64236.
MGIMGI:2384850. Pdlim2.

Phylogenomic databases

eggNOGNOG246155.
GeneTreeENSGT00590000082980.
HOGENOMHOG000290704.
HOVERGENHBG061371.
InParanoidQ8R1G6.
OMAFQSLAHS.
OrthoDBEOG77DJ69.
PhylomeDBQ8R1G6.
TreeFamTF106408.

Gene expression databases

ArrayExpressQ8R1G6.
BgeeQ8R1G6.
CleanExMM_PDLIM2.
GenevestigatorQ8R1G6.

Family and domain databases

Gene3D2.10.110.10. 1 hit.
2.30.42.10. 1 hit.
InterProIPR001478. PDZ.
IPR001781. Znf_LIM.
[Graphical view]
PfamPF00412. LIM. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTSM00132. LIM. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 1 hit.
PROSITEPS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8R1G6.
NextBio373818.
PROQ8R1G6.
SOURCESearch...

Entry information

Entry namePDLI2_MOUSE
AccessionPrimary (citable) accession number: Q8R1G6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot