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Q8R1G6

- PDLI2_MOUSE

UniProt

Q8R1G6 - PDLI2_MOUSE

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Protein
PDZ and LIM domain protein 2
Gene
Pdlim2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity By similarity.

GO - Molecular functioni

  1. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
PDZ and LIM domain protein 2
Alternative name(s):
PDZ-LIM protein mystique
Gene namesi
Name:Pdlim2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:2384850. Pdlim2.

Subcellular locationi

Cytoplasm. Cytoplasmcytoskeleton
Note: Localizes at the cytoskeleton. Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin (PXN) By similarity.

GO - Cellular componenti

  1. actin cytoskeleton Source: Ensembl
  2. cytoplasm Source: UniProtKB-SubCell
  3. focal adhesion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349PDZ and LIM domain protein 2
PRO_0000075864Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991Phosphoserine1 Publication
Modified residuei205 – 2051Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8R1G6.
PaxDbiQ8R1G6.
PRIDEiQ8R1G6.

PTM databases

PhosphoSiteiQ8R1G6.

Expressioni

Tissue specificityi

Highly expressed in lung. Expressed at intermediate level in kidney, testis and spleen. Weakly expressed in heart and brain.1 Publication

Inductioni

Regulated by IGF-1.1 Publication

Gene expression databases

ArrayExpressiQ8R1G6.
BgeeiQ8R1G6.
CleanExiMM_PDLIM2.
GenevestigatoriQ8R1G6.

Interactioni

Subunit structurei

Interacts with alpha-actinins ACTN1 and ACTN4, FLNA and MYH9 By similarity.

Protein-protein interaction databases

BioGridi229389. 2 interactions.
IntActiQ8R1G6. 2 interactions.
MINTiMINT-4118594.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 75
Beta strandi9 – 113
Beta strandi18 – 203
Turni21 – 244
Beta strandi25 – 284
Helixi38 – 414
Beta strandi48 – 525
Helixi62 – 709
Beta strandi74 – 818

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VB7NMR-A3-83[»]
ProteinModelPortaliQ8R1G6.
SMRiQ8R1G6. Positions 2-89, 279-344.

Miscellaneous databases

EvolutionaryTraceiQ8R1G6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8484PDZ
Add
BLAST
Domaini281 – 34161LIM zinc-binding
Add
BLAST

Sequence similaritiesi

Contains 1 PDZ (DHR) domain.

Keywords - Domaini

LIM domain

Phylogenomic databases

eggNOGiNOG246155.
GeneTreeiENSGT00590000082980.
HOGENOMiHOG000290704.
HOVERGENiHBG061371.
InParanoidiQ8R1G6.
OMAiFQSLAHS.
OrthoDBiEOG77DJ69.
PhylomeDBiQ8R1G6.
TreeFamiTF106408.

Family and domain databases

Gene3Di2.10.110.10. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced.

Isoform 1 (identifier: Q8R1G6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MALTVDVAGP APWGFRISGG RDFHTPIIVT KVTERGKAEA ADLRPGDIIV    50
AINGQSAENM LHAEAQSKIR QSASPLRLQL DRSQTASPGQ TNGEGSLEVL 100
ATRFQGSLRT HRDSQSSQRS ACFSPVSLSP RPCSPFSTPP PTSPVALSKE 150
DMIGCSFQSL THSPGLAAAH HLTYPGHPTS QQAGHSSPSD SAVRVLLHSP 200
GRPSSPRFSS LDLEEDSEVF KMLQENRQGR AAPRQSSSFR LLQEALEAEE 250
RGGTPAFVPS SLSSQASLPT SRALATPPKL HTCEKCSVNI SNQAVRIQEG 300
RYRHPGCYTC ADCGLNLKMR GHFWVGNELY CEKHARQRYS MPGTLNSRA 349
Length:349
Mass (Da):37,703
Last modified:June 1, 2002 - v1
Checksum:i9809D7F1AF2BD907
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC024556 mRNA. Translation: AAH24556.1.
CCDSiCCDS27248.1. [Q8R1G6-1]
RefSeqiNP_001240665.1. NM_001253736.1. [Q8R1G6-1]
NP_666090.1. NM_145978.2. [Q8R1G6-1]
UniGeneiMm.283968.

Genome annotation databases

EnsembliENSMUST00000022681; ENSMUSP00000022681; ENSMUSG00000022090. [Q8R1G6-1]
ENSMUST00000153735; ENSMUSP00000116200; ENSMUSG00000022090. [Q8R1G6-1]
GeneIDi213019.
KEGGimmu:213019.
UCSCiuc007uni.1. mouse. [Q8R1G6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC024556 mRNA. Translation: AAH24556.1 .
CCDSi CCDS27248.1. [Q8R1G6-1 ]
RefSeqi NP_001240665.1. NM_001253736.1. [Q8R1G6-1 ]
NP_666090.1. NM_145978.2. [Q8R1G6-1 ]
UniGenei Mm.283968.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VB7 NMR - A 3-83 [» ]
ProteinModelPortali Q8R1G6.
SMRi Q8R1G6. Positions 2-89, 279-344.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 229389. 2 interactions.
IntActi Q8R1G6. 2 interactions.
MINTi MINT-4118594.

PTM databases

PhosphoSitei Q8R1G6.

Proteomic databases

MaxQBi Q8R1G6.
PaxDbi Q8R1G6.
PRIDEi Q8R1G6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022681 ; ENSMUSP00000022681 ; ENSMUSG00000022090 . [Q8R1G6-1 ]
ENSMUST00000153735 ; ENSMUSP00000116200 ; ENSMUSG00000022090 . [Q8R1G6-1 ]
GeneIDi 213019.
KEGGi mmu:213019.
UCSCi uc007uni.1. mouse. [Q8R1G6-1 ]

Organism-specific databases

CTDi 64236.
MGIi MGI:2384850. Pdlim2.

Phylogenomic databases

eggNOGi NOG246155.
GeneTreei ENSGT00590000082980.
HOGENOMi HOG000290704.
HOVERGENi HBG061371.
InParanoidi Q8R1G6.
OMAi FQSLAHS.
OrthoDBi EOG77DJ69.
PhylomeDBi Q8R1G6.
TreeFami TF106408.

Miscellaneous databases

EvolutionaryTracei Q8R1G6.
NextBioi 373818.
PROi Q8R1G6.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8R1G6.
Bgeei Q8R1G6.
CleanExi MM_PDLIM2.
Genevestigatori Q8R1G6.

Family and domain databases

Gene3Di 2.10.110.10. 1 hit.
2.30.42.10. 1 hit.
InterProi IPR001478. PDZ.
IPR001781. Znf_LIM.
[Graphical view ]
Pfami PF00412. LIM. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view ]
SMARTi SM00132. LIM. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view ]
SUPFAMi SSF50156. SSF50156. 1 hit.
PROSITEi PS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Colon.
  2. "Mystique is a new IGF-I regulated PDZ-LIM domain protein that promotes cell attachment and migration and suppresses anchorage independent growth."
    Loughran G., Healy N., Kiely P.A., Huigsloot M., Kedersha N.L., O'connor R.
    Mol. Biol. Cell 16:1811-1822(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  3. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-205, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  4. "Solution structure of the PDZ domain of PDZ and LIM domain 2."
    RIKEN structural genomics initiative (RSGI)
    Submitted (FEB-2004) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 3-83.

Entry informationi

Entry nameiPDLI2_MOUSE
AccessioniPrimary (citable) accession number: Q8R1G6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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