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Q8R1G6

- PDLI2_MOUSE

UniProt

Q8R1G6 - PDLI2_MOUSE

Protein

PDZ and LIM domain protein 2

Gene

Pdlim2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity By similarity.By similarity

    GO - Molecular functioni

    1. zinc ion binding Source: InterPro

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    PDZ and LIM domain protein 2
    Alternative name(s):
    PDZ-LIM protein mystique
    Gene namesi
    Name:Pdlim2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:2384850. Pdlim2.

    Subcellular locationi

    Cytoplasm. Cytoplasmcytoskeleton
    Note: Localizes at the cytoskeleton. Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin (PXN) By similarity.By similarity

    GO - Cellular componenti

    1. actin cytoskeleton Source: Ensembl
    2. cytoplasm Source: UniProtKB-SubCell
    3. focal adhesion Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 349349PDZ and LIM domain protein 2PRO_0000075864Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei199 – 1991Phosphoserine1 Publication
    Modified residuei205 – 2051Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8R1G6.
    PaxDbiQ8R1G6.
    PRIDEiQ8R1G6.

    PTM databases

    PhosphoSiteiQ8R1G6.

    Expressioni

    Tissue specificityi

    Highly expressed in lung. Expressed at intermediate level in kidney, testis and spleen. Weakly expressed in heart and brain.1 Publication

    Inductioni

    Regulated by IGF-1.1 Publication

    Gene expression databases

    ArrayExpressiQ8R1G6.
    BgeeiQ8R1G6.
    CleanExiMM_PDLIM2.
    GenevestigatoriQ8R1G6.

    Interactioni

    Subunit structurei

    Interacts with alpha-actinins ACTN1 and ACTN4, FLNA and MYH9.By similarity

    Protein-protein interaction databases

    BioGridi229389. 2 interactions.
    IntActiQ8R1G6. 2 interactions.
    MINTiMINT-4118594.

    Structurei

    Secondary structure

    1
    349
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 75
    Beta strandi9 – 113
    Beta strandi18 – 203
    Turni21 – 244
    Beta strandi25 – 284
    Helixi38 – 414
    Beta strandi48 – 525
    Helixi62 – 709
    Beta strandi74 – 818

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VB7NMR-A3-83[»]
    ProteinModelPortaliQ8R1G6.
    SMRiQ8R1G6. Positions 2-89, 279-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8R1G6.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 8484PDZPROSITE-ProRule annotationAdd
    BLAST
    Domaini281 – 34161LIM zinc-bindingPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 LIM zinc-binding domain.PROSITE-ProRule annotation
    Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    LIM domain

    Phylogenomic databases

    eggNOGiNOG246155.
    GeneTreeiENSGT00590000082980.
    HOGENOMiHOG000290704.
    HOVERGENiHBG061371.
    InParanoidiQ8R1G6.
    OMAiFQSLAHS.
    OrthoDBiEOG77DJ69.
    PhylomeDBiQ8R1G6.
    TreeFamiTF106408.

    Family and domain databases

    Gene3Di2.10.110.10. 1 hit.
    2.30.42.10. 1 hit.
    InterProiIPR001478. PDZ.
    IPR001781. Znf_LIM.
    [Graphical view]
    PfamiPF00412. LIM. 1 hit.
    PF00595. PDZ. 1 hit.
    [Graphical view]
    SMARTiSM00132. LIM. 1 hit.
    SM00228. PDZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 1 hit.
    PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
    PS50023. LIM_DOMAIN_2. 1 hit.
    PS50106. PDZ. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced.

    Isoform 1 (identifier: Q8R1G6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MALTVDVAGP APWGFRISGG RDFHTPIIVT KVTERGKAEA ADLRPGDIIV    50
    AINGQSAENM LHAEAQSKIR QSASPLRLQL DRSQTASPGQ TNGEGSLEVL 100
    ATRFQGSLRT HRDSQSSQRS ACFSPVSLSP RPCSPFSTPP PTSPVALSKE 150
    DMIGCSFQSL THSPGLAAAH HLTYPGHPTS QQAGHSSPSD SAVRVLLHSP 200
    GRPSSPRFSS LDLEEDSEVF KMLQENRQGR AAPRQSSSFR LLQEALEAEE 250
    RGGTPAFVPS SLSSQASLPT SRALATPPKL HTCEKCSVNI SNQAVRIQEG 300
    RYRHPGCYTC ADCGLNLKMR GHFWVGNELY CEKHARQRYS MPGTLNSRA 349
    Length:349
    Mass (Da):37,703
    Last modified:June 1, 2002 - v1
    Checksum:i9809D7F1AF2BD907
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC024556 mRNA. Translation: AAH24556.1.
    CCDSiCCDS27248.1. [Q8R1G6-1]
    RefSeqiNP_001240665.1. NM_001253736.1. [Q8R1G6-1]
    NP_666090.1. NM_145978.2. [Q8R1G6-1]
    UniGeneiMm.283968.

    Genome annotation databases

    EnsembliENSMUST00000022681; ENSMUSP00000022681; ENSMUSG00000022090. [Q8R1G6-1]
    ENSMUST00000153735; ENSMUSP00000116200; ENSMUSG00000022090. [Q8R1G6-1]
    GeneIDi213019.
    KEGGimmu:213019.
    UCSCiuc007uni.1. mouse. [Q8R1G6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC024556 mRNA. Translation: AAH24556.1 .
    CCDSi CCDS27248.1. [Q8R1G6-1 ]
    RefSeqi NP_001240665.1. NM_001253736.1. [Q8R1G6-1 ]
    NP_666090.1. NM_145978.2. [Q8R1G6-1 ]
    UniGenei Mm.283968.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VB7 NMR - A 3-83 [» ]
    ProteinModelPortali Q8R1G6.
    SMRi Q8R1G6. Positions 2-89, 279-344.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 229389. 2 interactions.
    IntActi Q8R1G6. 2 interactions.
    MINTi MINT-4118594.

    PTM databases

    PhosphoSitei Q8R1G6.

    Proteomic databases

    MaxQBi Q8R1G6.
    PaxDbi Q8R1G6.
    PRIDEi Q8R1G6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022681 ; ENSMUSP00000022681 ; ENSMUSG00000022090 . [Q8R1G6-1 ]
    ENSMUST00000153735 ; ENSMUSP00000116200 ; ENSMUSG00000022090 . [Q8R1G6-1 ]
    GeneIDi 213019.
    KEGGi mmu:213019.
    UCSCi uc007uni.1. mouse. [Q8R1G6-1 ]

    Organism-specific databases

    CTDi 64236.
    MGIi MGI:2384850. Pdlim2.

    Phylogenomic databases

    eggNOGi NOG246155.
    GeneTreei ENSGT00590000082980.
    HOGENOMi HOG000290704.
    HOVERGENi HBG061371.
    InParanoidi Q8R1G6.
    OMAi FQSLAHS.
    OrthoDBi EOG77DJ69.
    PhylomeDBi Q8R1G6.
    TreeFami TF106408.

    Miscellaneous databases

    EvolutionaryTracei Q8R1G6.
    NextBioi 373818.
    PROi Q8R1G6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8R1G6.
    Bgeei Q8R1G6.
    CleanExi MM_PDLIM2.
    Genevestigatori Q8R1G6.

    Family and domain databases

    Gene3Di 2.10.110.10. 1 hit.
    2.30.42.10. 1 hit.
    InterProi IPR001478. PDZ.
    IPR001781. Znf_LIM.
    [Graphical view ]
    Pfami PF00412. LIM. 1 hit.
    PF00595. PDZ. 1 hit.
    [Graphical view ]
    SMARTi SM00132. LIM. 1 hit.
    SM00228. PDZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 1 hit.
    PROSITEi PS00478. LIM_DOMAIN_1. 1 hit.
    PS50023. LIM_DOMAIN_2. 1 hit.
    PS50106. PDZ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Colon.
    2. "Mystique is a new IGF-I regulated PDZ-LIM domain protein that promotes cell attachment and migration and suppresses anchorage independent growth."
      Loughran G., Healy N., Kiely P.A., Huigsloot M., Kedersha N.L., O'connor R.
      Mol. Biol. Cell 16:1811-1822(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
    3. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-205, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    4. "Solution structure of the PDZ domain of PDZ and LIM domain 2."
      RIKEN structural genomics initiative (RSGI)
      Submitted (FEB-2004) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 3-83.

    Entry informationi

    Entry nameiPDLI2_MOUSE
    AccessioniPrimary (citable) accession number: Q8R1G6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3