PDZ and LIM domain protein 2 - Mus musculus (Mouse)

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Q8R1G6 (PDLI2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 91. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
PDZ and LIM domain protein 2

Alternative name(s):
PDZ-LIM protein mystique

Gene names

Name:

Pdlim2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity By similarity.
Subunit structure	Interacts with alpha-actinins ACTN1 and ACTN4, FLNA and MYH9 By similarity.
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton. Note: Localizes at the cytoskeleton. Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin (PXN) By similarity.
Tissue specificity	Highly expressed in lung. Expressed at intermediate level in kidney, testis and spleen. Weakly expressed in heart and brain. Ref.2
Induction	Regulated by IGF-1. Ref.2
Sequence similarities	Contains 1 LIM zinc-binding domain. Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Domain	LIM domain
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	actin cytoskeleton Inferred from electronic annotation. Source: Compara cell surface Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell focal adhesion Inferred from electronic annotation. Source: Compara
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced.

Isoform 1 (identifier: Q8R1G6-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 349

349

PDZ and LIM domain protein 2

PRO_0000075864

Regions

Domain

1 – 84

PDZ

Domain

281 – 341

LIM zinc-binding

Amino acid modifications

Modified residue

199

Phosphoserine Ref.3 Ref.4

Modified residue

205

Phosphoserine Ref.4

Secondary structure

349

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 9809D7F1AF2BD907
Show »

FASTA

349

37,703

        10         20         30         40         50         60 
MALTVDVAGP APWGFRISGG RDFHTPIIVT KVTERGKAEA ADLRPGDIIV AINGQSAENM 

        70         80         90        100        110        120 
LHAEAQSKIR QSASPLRLQL DRSQTASPGQ TNGEGSLEVL ATRFQGSLRT HRDSQSSQRS 

       130        140        150        160        170        180 
ACFSPVSLSP RPCSPFSTPP PTSPVALSKE DMIGCSFQSL THSPGLAAAH HLTYPGHPTS 

       190        200        210        220        230        240 
QQAGHSSPSD SAVRVLLHSP GRPSSPRFSS LDLEEDSEVF KMLQENRQGR AAPRQSSSFR 

       250        260        270        280        290        300 
LLQEALEAEE RGGTPAFVPS SLSSQASLPT SRALATPPKL HTCEKCSVNI SNQAVRIQEG 

       310        320        330        340 
RYRHPGCYTC ADCGLNLKMR GHFWVGNELY CEKHARQRYS MPGTLNSRA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Colon.
[2]	"Mystique is a new IGF-I regulated PDZ-LIM domain protein that promotes cell attachment and migration and suppresses anchorage independent growth." Loughran G., Healy N., Kiely P.A., Huigsloot M., Kedersha N.L., O'connor R. Mol. Biol. Cell 16:1811-1822(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[3]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, MASS SPECTROMETRY. Tissue: Melanoma.
[4]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-205, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
[5]	"Solution structure of the PDZ domain of PDZ and LIM domain 2." RIKEN structural genomics initiative (RSGI) Submitted (FEB-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 3-83.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BC024556 mRNA. Translation: AAH24556.1.

IPI

IPI00153375.

RefSeq

NP_001240665.1. NM_001253736.1.
NP_666090.1. NM_145978.2.

UniGene

Mm.283968.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VB7	NMR	-	A	3-83	[»]

ProteinModelPortal

Q8R1G6.

SMR

Q8R1G6. Positions 2-89, 279-344.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-4118594.

PTM databases

PhosphoSite

Q8R1G6.

Proteomic databases

PaxDb

Q8R1G6.

PRIDE

Q8R1G6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000022681; ENSMUSP00000022681; ENSMUSG00000022090.
ENSMUST00000153735; ENSMUSP00000116200; ENSMUSG00000022090.

GeneID

213019.

KEGG

mmu:213019.

UCSC

uc007uni.1. mouse.

Organism-specific databases

CTD

64236.

MGI

MGI:2384850. Pdlim2.

Phylogenomic databases

eggNOG

NOG246155.

GeneTree

ENSGT00590000082980.

HOGENOM

HOG000290704.

HOVERGEN

HBG061371.

InParanoid

Q8R1G6.

OMA

FQSLAHS.

Gene expression databases

ArrayExpress

Q8R1G6.

Bgee

Q8R1G6.

CleanEx

MM_PDLIM2.

Genevestigator

Q8R1G6.

GermOnline

ENSMUSG00000022090. Mus musculus.

Family and domain databases

Gene3D

2.10.110.10. 1 hit.

InterPro

IPR001478. PDZ.
IPR001781. Znf_LIM.
[Graphical view]

Pfam

PF00412. LIM. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]

SMART

SM00132. LIM. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]

SUPFAM

SSF50156. PDZ. 1 hit.

PROSITE

PS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8R1G6.

NextBio

373818.

SOURCE

Search...

Entry information

Entry name

PDLI2_MOUSE

Accession

Primary (citable) accession number: Q8R1G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents