Q8R1B8 (RORB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear receptor ROR-beta

Alternative name(s):
Nuclear receptor RZR-beta
Nuclear receptor subfamily 1 group F member 2
Retinoid-related orphan receptor-beta

Gene names

Name:	Rorb
Synonyms:	Nr1f2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Orphan nuclear receptor required for normal postnatal development of rod and cone photoreceptor cells. Regulates transcription of OPN1SW in cone photoreceptor cells by binding the sequence 5'-AGGTCA-3' in the OPN1SW promoter. Ref.1 Ref.4
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in inner and outer neuroblastic layer as well as in the ganglion cell layer of the developing retina. Ref.1
Developmental stage	Highest level in retina of stage E17.5 embryos. Lower level in postnatal and adult retina. Ref.1
Disruption phenotype	Mice are blind, show juvenile ataxia, adult gait pecularities as well as delayed onset of male fertility. They show degeneration of the retina during postnatal development with severe defects in photoreceptor cell morphology as well as failure to express Opn1sw in cone photoreceptor cells. Ref.1 Ref.4
Sequence similarities	Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Sequence caution	The sequence AAH24842.2 differs from that shown. Reason: Intron retention. The sequence AAH58269.1 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Biological process	Sensory transduction Transcription Transcription regulation Vision
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Developmental protein Receptor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	eye photoreceptor cell development Inferred from mutant phenotype Ref.1. Source: UniProtKB intracellular receptor mediated signaling pathway Inferred from electronic annotation. Source: GOC positive regulation of transcription, DNA-dependent Inferred from direct assay Ref.1. Source: UniProtKB visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred by curator Ref.1. Source: UniProtKB
Molecular_function	ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from direct assay Ref.1. Source: UniProtKB sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.1. Source: UniProtKB steroid hormone receptor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: Q8R1B8-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q8R1B8-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MCENQPKTKADGT → MR

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Nuclear receptor ROR-beta		PRO_0000053515
Regions
	DNA binding	18 – 93	76	Nuclear receptor
	Zinc finger	21 – 41	21	NR C4-type
	Zinc finger	57 – 81	25	NR C4-type
	Region	94 – 221	128	Hinge Potential
	Region	222 – 470	249	Ligand-binding Potential
Natural variations
	Alternative sequence	1 – 13	13	MCENQ…KADGT → MR in isoform 1.		VSP_022576
Experimental info
	Sequence conflict	107	1	E → G in BAE93688. Ref.2
	Sequence conflict	220	1	M → L in BAE93688. Ref.2
	Sequence conflict	247	1	Q → H in BAE93688. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 6E2F30DB56E94B86	FASTA	470	53,118
	10 20 30 40 50 60 MCENQPKTKA DGTAQIEVIP CKICGDKSSG IHYGVITCEG CKGFFRRSQQ NNASYSCPRQ 70 80 90 100 110 120 RNCLIDRTNR NRCQHCRLQK CLALGMSRDA VKFGRMSKKQ RDSLYAEVQK HQQRLQEQRQ 130 140 150 160 170 180 QQSGEAEALA RVYSSSISNG LSNLNTETGG TYANGHVIDL PKSEGYYSID SGQPSPDQSG 190 200 210 220 230 240 LDMTGIKQIK QEPIYDLTSV PNLFTYSSFN NGQLAPGITM SEIDRIAQNI IKSHLETCQY 250 260 270 280 290 300 TMEELHQLAW QTHTYEEIKA YQSKSREALW QQCAIQITHA IQYVVEFAKR ITGFMELCQN 310 320 330 340 350 360 DQILLLKSGC LEVVLVRMCR AFNPLNNTVL FEGKYGGMQM FKALGSDDLV NEAFDFAKNL 370 380 390 400 410 420 CSLQLTEEEI ALFSSAVLIS PDRAWLIEPR KVQKLQEKIY FALQHVIQKN HLDDETLAKL 430 440 450 460 470 IAKIPTITAV CNLHGEKLQV FKQSHPDIVN TLFPPLYKEL FNPDCAAVCK
	Isoform 1 [UniParc]. Checksum: 8A22F5C8D104B269	FASTA	459	52,000
	10 20 30 40 50 60 MRAQIEVIPC KICGDKSSGI HYGVITCEGC KGFFRRSQQN NASYSCPRQR NCLIDRTNRN 70 80 90 100 110 120 RCQHCRLQKC LALGMSRDAV KFGRMSKKQR DSLYAEVQKH QQRLQEQRQQ QSGEAEALAR 130 140 150 160 170 180 VYSSSISNGL SNLNTETGGT YANGHVIDLP KSEGYYSIDS GQPSPDQSGL DMTGIKQIKQ 190 200 210 220 230 240 EPIYDLTSVP NLFTYSSFNN GQLAPGITMS EIDRIAQNII KSHLETCQYT MEELHQLAWQ 250 260 270 280 290 300 THTYEEIKAY QSKSREALWQ QCAIQITHAI QYVVEFAKRI TGFMELCQND QILLLKSGCL 310 320 330 340 350 360 EVVLVRMCRA FNPLNNTVLF EGKYGGMQMF KALGSDDLVN EAFDFAKNLC SLQLTEEEIA 370 380 390 400 410 420 LFSSAVLISP DRAWLIEPRK VQKLQEKIYF ALQHVIQKNH LDDETLAKLI AKIPTITAVC 430 440 450 NLHGEKLQVF KQSHPDIVNT LFPPLYKELF NPDCAAVCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Activation of the blue opsin gene in cone photoreceptor development by retinoid-related orphan receptor beta." Srinivas M., Ng L., Liu H., Jia L., Forrest D. Mol. Endocrinol. 20:1728-1741(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE. Strain: C57BL/6. Tissue: Embryonic eye.
[2]	"Mouse ROR beta partial cDNA." Takano A., Katsuyama Y. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Retina.
[4]	"Disruption of retinoid-related orphan receptor beta changes circadian behavior, causes retinal degeneration and leads to vacillans phenotype in mice." Andre E., Conquet F., Steinmayr M., Stratton S.C., Porciatti V., Becker-Andre M. EMBO J. 17:3867-3877(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC024842 mRNA. Translation: AAH24842.2. Sequence problems. BC058269 mRNA. Translation: AAH58269.1. Sequence problems. DQ779923 mRNA. Translation: ABG77269.1. DQ779924 mRNA. Translation: ABG77270.1. AB258405 mRNA. Translation: BAE93688.1.
IPI	IPI00153341. IPI00788322.
RefSeq	NP_001036819.1. NM_001043354.2. NP_666207.3. NM_146095.4.
UniGene	Mm.276747.
3D structure databases
ProteinModelPortal	Q8R1B8.
SMR	Q8R1B8. Positions 19-139, 219-462.
ModBase	Search...
PTM databases
PhosphoSite	Q8R1B8.
Proteomic databases
PRIDE	Q8R1B8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000040153; ENSMUSP00000047597; ENSMUSG00000036192. ENSMUST00000112828; ENSMUSP00000108447; ENSMUSG00000036192. ENSMUST00000112832; ENSMUSP00000108451; ENSMUSG00000036192.
GeneID	225998.
KEGG	mmu:225998.
UCSC	uc008gyc.1. mouse. uc008gyd.1. mouse.
Organism-specific databases
CTD	6096.
MGI	MGI:1343464. Rorb.
Phylogenomic databases
eggNOG	NOG324222.
GeneTree	ENSGT00700000104109.
HOGENOM	HOG000010200.
HOVERGEN	HBG106848.
KO	K08533.
OMA	SGHTMRA.
OrthoDB	EOG4KH2TT.
Gene expression databases
Bgee	Q8R1B8.
CleanEx	MM_RORB.
Genevestigator	Q8R1B8.
GermOnline	ENSMUSG00000036192. Mus musculus.
Family and domain databases
Gene3D	1.10.565.10. 2 hits. 3.30.50.10. 1 hit.
InterPro	IPR008946. Nucl_hormone_rcpt_ligand-bd. IPR000536. Nucl_hrmn_rcpt_lig-bd_core. IPR003079. ROR_rcpt. IPR001723. Str_hrmn_rcpt. IPR001628. Znf_hrmn_rcpt. IPR013088. Znf_NHR/GATA. [Graphical view]
Pfam	PF00104. Hormone_recep. 1 hit. PF00105. zf-C4. 1 hit. [Graphical view]
PRINTS	PR01293. RORNUCRECPTR. PR00398. STRDHORMONER. PR00047. STROIDFINGER.
SMART	SM00430. HOLI. 1 hit. SM00399. ZnF_C4. 1 hit. [Graphical view]
SUPFAM	SSF48508. Str_ncl_receptor. 1 hit.
PROSITE	PS00031. NUCLEAR_REC_DBD_1. 1 hit. PS51030. NUCLEAR_REC_DBD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	377911.
SOURCE	Search...

Entry information

Entry name

RORB_MOUSE

Accession

Primary (citable) accession number: Q8R1B8
Secondary accession number(s): Q0PQZ2, Q0PQZ3, Q1MVR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program