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Protein

Dedicator of cytokinesis protein 7

Gene

Dock7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a guanine nucleotide exchange factor (GEF), which activates Rac1 and Rac3 Rho small GTPases by exchanging bound GDP for free GTP. Does not have a GEF activity for CDC42. Required for STMN1 'Ser-15' phosphorylation during axon formation and consequently for neuronal polarization (By similarity). Has a role in pigmentation (PubMed:19202056). Involved in the regulation of cortical neurogenesis through the control of radial glial cells (RGCs) proliferation versus differentiation; negatively regulates the basal-to-apical interkinetic nuclear migration of RGCs by antagonizing the microtubule growth-promoting function of TACC3 (PubMed:22842144).By similarity2 Publications

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: UniProtKB-KW
  • Rac GTPase binding Source: BHF-UCL

GO - Biological processi

  • activation of GTPase activity Source: MGI
  • axonogenesis Source: MGI
  • establishment of neuroblast polarity Source: MGI
  • hematopoietic progenitor cell differentiation Source: MGI
  • interkinetic nuclear migration Source: UniProtKB
  • microtubule cytoskeleton organization Source: MGI
  • neuron projection development Source: MGI
  • pigmentation Source: MGI
  • positive regulation of peptidyl-serine phosphorylation Source: MGI
  • positive regulation of vascular associated smooth muscle cell migration Source: MGI
  • regulation of neurogenesis Source: UniProtKB
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Guanine-nucleotide releasing factor

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Dedicator of cytokinesis protein 7
Alternative name(s):
Protein moonlight1 Publication
Gene namesi
Name:Dock7
Synonyms:Gm430, Kiaa1771, Mnlt1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1914549. Dock7.

Subcellular locationi

  • Cell projectionaxon By similarity

  • Note: Enriched in the developing axons of hippocampal neurons.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection

Pathology & Biotechi

Involvement in diseasei

Defects in Dock7 are responsible for the moonlight phenotype (mnlt). mnlt/mnlt mice display an overall lightened coat color and hypopigmentation of the belly and distal extremities, particularly the paws, tail tip, and genitalia.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001899961 – 2130Dedicator of cytokinesis protein 7Add BLAST2130

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei30PhosphoserineCombined sources1
Modified residuei180PhosphoserineBy similarity1
Modified residuei182PhosphoserineCombined sources1
Modified residuei381N6-methyllysineBy similarity1
Modified residuei450PhosphothreonineCombined sources1
Modified residuei452PhosphoserineBy similarity1
Modified residuei862PhosphoserineCombined sources1
Modified residuei864PhosphoserineCombined sources1
Modified residuei882PhosphoserineBy similarity1
Modified residuei888PhosphoserineBy similarity1
Modified residuei896PhosphoserineCombined sources1
Modified residuei900PhosphoserineCombined sources1
Modified residuei905PhosphoserineBy similarity1
Modified residuei907PhosphothreonineCombined sources1
Modified residuei909PhosphothreonineBy similarity1
Modified residuei910PhosphoserineCombined sources1
Modified residuei929PhosphoserineCombined sources1
Modified residuei963PhosphoserineBy similarity1
Modified residuei1382PhosphoserineBy similarity1
Modified residuei1420PhosphoserineCombined sources1
Modified residuei1422PhosphoserineCombined sources1
Modified residuei1424PhosphoserineCombined sources1
Modified residuei1428PhosphoserineCombined sources1
Modified residuei1952N6-acetyllysineCombined sources1
Modified residuei2119PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ8R1A4.
PeptideAtlasiQ8R1A4.
PRIDEiQ8R1A4.

PTM databases

iPTMnetiQ8R1A4.
PhosphoSitePlusiQ8R1A4.

Expressioni

Gene expression databases

CleanExiMM_DOCK7.

Interactioni

Subunit structurei

Interacts with TSC1 (By similarity). Interacts with nucleotide-free RAC1 and RAC3 (By similarity). Interacts with TACC3. Interacts with CRY1.By similarity2 Publications

GO - Molecular functioni

  • Rac GTPase binding Source: BHF-UCL

Protein-protein interaction databases

IntActiQ8R1A4. 4 interactors.
MINTiMINT-4093415.
STRINGi10090.ENSMUSP00000075233.

Structurei

3D structure databases

ProteinModelPortaliQ8R1A4.
SMRiQ8R1A4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini561 – 727DHR-1PROSITE-ProRule annotationAdd BLAST167
Domaini1668 – 2104DHR-2PROSITE-ProRule annotationAdd BLAST437

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili365 – 395Sequence analysisAdd BLAST31
Coiled coili2076 – 2102Sequence analysisAdd BLAST27

Domaini

The DHR-2 domain mediates GEF activity.By similarity

Sequence similaritiesi

Belongs to the DOCK family.PROSITE-ProRule annotation
Contains 1 DHR-1 domain.PROSITE-ProRule annotation
Contains 1 DHR-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1997. Eukaryota.
ENOG410XNVY. LUCA.
HOGENOMiHOG000230910.
HOVERGENiHBG051390.
InParanoidiQ8R1A4.
PhylomeDBiQ8R1A4.

Family and domain databases

InterProiIPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR021816. DOCK_C/D_N.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 4 hits.
PfamiPF06920. DHR-2. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF11878. DUF3398. 1 hit.
[Graphical view]
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R1A4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAERRAFAQK ISRTVAAEVR KQISGQYSGS PQLLKNLNIV GNISHHTTVP
60 70 80 90 100
LTEAVDPVDL EDYLVTHPLS GDSGPLRDLV EFPPDDIEVV YSPRDCRTLV
110 120 130 140 150
SAVPEESEMD PHVRDCIRSY TEDWAVVVRK YHKLGTGFNP NTLDKQKERQ
160 170 180 190 200
KGLPRQVFES DEAPDGSSYQ DEQDDLKRRS MSIDDTPRGS WACSIFDLKN
210 220 230 240 250
SLPDALLPNL LDRTPNEEID HQNDDQRKSN RHKELFALHP SPDEEEPIER
260 270 280 290 300
LSVPDVPKEH FGQRLLVKCL SLKFEIEIEP IFASLALYDV KEKKKISENF
310 320 330 340 350
YFDLNSEQMK GLLRPHVPPA AITTLARSAI FSITYPSQDV FLVIKLEKVL
360 370 380 390 400
QQGDIGECAE PYMIFKEADA TKNKEKLEKL KSQADQFCQR LGKYRMPFAW
410 420 430 440 450
TAIHLMNIVS SAGSLERDST EVEISTGERK GSWSERRNSS LVGRRSLERT
460 470 480 490 500
TSGDDACNLT SFRPATLTVA NFFKQEGDRL SDEDLYKFLA DMRRPSSVLR
510 520 530 540 550
RLRPITAQLK IDISPAPENP HYCLTPELLQ VKLYPDSRVR PTREILEFPA
560 570 580 590 600
RDVYVPNTTY RNLLYIYPQS LNFANRQGSA RNITVKVQFM YGEDPSNAMP
610 620 630 640 650
VIFGKSSCSE FSKEAYTAVV YHNRSPDFHE EIKVKLPATL TDHHHLLFTF
660 670 680 690 700
YHVSCQQKQN TPLETPVGYT WIPMLQNGRL KTGQFCLPVS LEKPPQAYSV
710 720 730 740 750
LSPEVPLPGM KWVDNHKGVF NVEVVAVSSI HTQDPYLDKF FALVNALDEH
760 770 780 790 800
MFPVRIGDMR IMENNLESEL KSSISALNSS QLEPVVRFLH LLLDKLILLV
810 820 830 840 850
VRPPVIAGQI VNLGQASFEA MASIINRLHK NLEGNHDQHG RNNLLASYIY
860 870 880 890 900
YVFRLPNTYP NSPSPGPGGL GGSVHYATMA RSAVRPASLN LNRSRSLSNS
910 920 930 940 950
NPDISGTPTS PDDEVRSIIG SKGLDRSNSW VNTGPKAAPW GSNPSPSAES
960 970 980 990 1000
TQAMDRSCNR MSSHTETSSF LQTLTGRLPT KKLFHEELAL QWVVCSGSVR
1010 1020 1030 1040 1050
ESALQQAWFF FELMVKSMVH HLYFNDKLDA PRESRFPERF MDDIAALVST
1060 1070 1080 1090 1100
IAGDVVSRFQ KDTEMVERLN TSLAFFLNDL LSVMDRGFVF SLIKSCYKQV
1110 1120 1130 1140 1150
SAKLYSLPNP SVLVSLRLDF LRIICSHEHY VTLNLPCSLL TPPASPSPSV
1160 1170 1180 1190 1200
SSATSQSSGF STSVQDQKIA NMFELSLPFR QQHYLAGLVL TELALILDPD
1210 1220 1230 1240 1250
AEGLFGLHKK VINMVHNLLS THDSDPRYSD PQIKARVAML YLPLIGIIME
1260 1270 1280 1290 1300
TVPQLYDFTE SHNQRGRPIC IAPDDYDSES GSMISQTVAM AIAGTSVPQL
1310 1320 1330 1340 1350
TRPGSFLLTS TSGRQHTTFS AESSRSLLIC LLWVLKNADE TVLQKWFTDL
1360 1370 1380 1390 1400
SVLQLNRLLD LLYLCVSCFE YKGKKVFERM NSLTFKKSKD MRAKLEEAIL
1410 1420 1430 1440 1450
GSIGARQEMV RRSRGQLERS PSGSAFGSQE NLRWRKDMTH WRQNSEKLDK
1460 1470 1480 1490 1500
SRAEIEHEAL IDGNLATEAN LIILDTLEII VQTVSVTESK ESILGGVLKV
1510 1520 1530 1540 1550
LLQSMACNQS AVYLQHCFAT QRALVSKFPE LLFEEETEQC ADLCLRLLRH
1560 1570 1580 1590 1600
CSSSISTIRS HASASLYLLM RQNFEIGNNF ARVKMQVTMS LSSLVGTSQN
1610 1620 1630 1640 1650
FNEEFLRRSL KTILTYAEED LELRETTFPD QVQDLVFNLH MILSDTVKMK
1660 1670 1680 1690 1700
EHQEDPEMLI DLMYRIAKGY QTSPDLRLTW LQNMAGKHSE RSNHAEAAQC
1710 1720 1730 1740 1750
LVHSAALVAE YLSMLEDRKY LPVGCVTFQN ISSNVLEESA VSDDVVSPDE
1760 1770 1780 1790 1800
EGICSGKYFT ESGLVGLLEQ AAASFSMAGM YEAVNEVYKV LIPIHEANRD
1810 1820 1830 1840 1850
AKKLSTIHGK LQEAFSKIVH QSTGWERMFG TYFRVGFYGT KFGDLDEQEF
1860 1870 1880 1890 1900
VYKEPAITKL AEISHRLEGF YGERFGEDVL EVIKDSNPVD KCKLDPNKAY
1910 1920 1930 1940 1950
IQITYVEPFF DTYEMKDRIT YFDKNYNLRR FMYCTPFTLD GRAHGELHEQ
1960 1970 1980 1990 2000
FKRKTILTTS HAFPYIKTRV NVTHKEEIIL TPIEVAIEDM QKKTQELAFA
2010 2020 2030 2040 2050
THQDPADPKM LQMVLQGSVG TTVNQGPLEV AQVFLSEIPG DPKLFRHHNK
2060 2070 2080 2090 2100
LRLCFKDFTK RCEDALRKNK SLIGPDQKEY QRELERNYHR LKEALQPLIN
2110 2120 2130
RKIPQLYKAV LPVTCHRDSF SRMSLRKMEL
Length:2,130
Mass (Da):241,438
Last modified:January 9, 2007 - v3
Checksum:i936B564638C1FC1D
GO
Isoform 2 (identifier: Q8R1A4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1822-1826: STGWE → DGK

Show »
Length:2,128
Mass (Da):241,178
Checksum:iF9EC4C5783631CE4
GO

Sequence cautioni

The sequence AAH24823 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH86672 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB28798 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0077081822 – 1826STGWE → DGK in isoform 2. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL627349 Genomic DNA. No translation available.
AL935325 Genomic DNA. No translation available.
DQ109674 mRNA. Translation: AAZ17650.1.
BC024823 mRNA. Translation: AAH24823.2. Different initiation.
BC024917 mRNA. Translation: AAH24917.3.
BC086672 mRNA. Translation: AAH86672.1. Different initiation.
AK122554 mRNA. Translation: BAC65836.1.
AK013336 mRNA. Translation: BAB28798.3. Different initiation.
AK014226 mRNA. Translation: BAB29215.1.
CCDSiCCDS71425.1. [Q8R1A4-2]
UniGeneiMm.260623.

Genome annotation databases

UCSCiuc008tup.2. mouse. [Q8R1A4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL627349 Genomic DNA. No translation available.
AL935325 Genomic DNA. No translation available.
DQ109674 mRNA. Translation: AAZ17650.1.
BC024823 mRNA. Translation: AAH24823.2. Different initiation.
BC024917 mRNA. Translation: AAH24917.3.
BC086672 mRNA. Translation: AAH86672.1. Different initiation.
AK122554 mRNA. Translation: BAC65836.1.
AK013336 mRNA. Translation: BAB28798.3. Different initiation.
AK014226 mRNA. Translation: BAB29215.1.
CCDSiCCDS71425.1. [Q8R1A4-2]
UniGeneiMm.260623.

3D structure databases

ProteinModelPortaliQ8R1A4.
SMRiQ8R1A4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R1A4. 4 interactors.
MINTiMINT-4093415.
STRINGi10090.ENSMUSP00000075233.

PTM databases

iPTMnetiQ8R1A4.
PhosphoSitePlusiQ8R1A4.

Proteomic databases

PaxDbiQ8R1A4.
PeptideAtlasiQ8R1A4.
PRIDEiQ8R1A4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc008tup.2. mouse. [Q8R1A4-1]

Organism-specific databases

MGIiMGI:1914549. Dock7.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1997. Eukaryota.
ENOG410XNVY. LUCA.
HOGENOMiHOG000230910.
HOVERGENiHBG051390.
InParanoidiQ8R1A4.
PhylomeDBiQ8R1A4.

Miscellaneous databases

ChiTaRSiDock7. mouse.
PROiQ8R1A4.
SOURCEiSearch...

Gene expression databases

CleanExiMM_DOCK7.

Family and domain databases

InterProiIPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR021816. DOCK_C/D_N.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 4 hits.
PfamiPF06920. DHR-2. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF11878. DUF3398. 1 hit.
[Graphical view]
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDOCK7_MOUSE
AccessioniPrimary (citable) accession number: Q8R1A4
Secondary accession number(s): Q45V78
, Q5PRE6, Q6PJ17, Q9CSB6, Q9CXM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.