Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Palmitoyltransferase ZDHHC3

Gene

Zdhhc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability (PubMed:15229235). Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi (PubMed:16129400). May also palmitoylate DLG4, DNAJC5 and SNAP25 (PubMed:15603741, PubMed:25253725).4 Publications

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri126 – 17651DHHC-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • protein palmitoylation Source: UniProtKB
  • protein targeting Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.3.1.225. 3474.

Protein family/group databases

TCDBi9.B.37.2.1. the huntington-interacting protein 14 (hip14) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyltransferase ZDHHC3Curated (EC:2.3.1.2254 Publications)
Alternative name(s):
GABA-A receptor-associated membrane protein 1Imported
Golgi-specific DHHC zinc finger protein1 Publication
Zinc finger DHHC domain-containing protein 3Imported
Short name:
DHHC-31 Publication
Gene namesi
Name:Zdhhc3Imported
Synonyms:Godz1 Publication, Gramp1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1926134. Zdhhc3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4747CytoplasmicSequence analysisAdd
BLAST
Transmembranei48 – 6821HelicalSequence analysisAdd
BLAST
Topological domaini69 – 724LumenalSequence analysis
Transmembranei73 – 9321HelicalSequence analysisAdd
BLAST
Topological domaini94 – 17178CytoplasmicSequence analysisAdd
BLAST
Transmembranei172 – 19221HelicalSequence analysisAdd
BLAST
Topological domaini193 – 21422LumenalSequence analysisAdd
BLAST
Transmembranei215 – 23521HelicalSequence analysisAdd
BLAST
Topological domaini236 – 29964CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi apparatus Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi157 – 1571C → S: Abolishes activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Palmitoyltransferase ZDHHC3PRO_0000212863Add
BLAST

Post-translational modificationi

Autopalmitoylated.3 Publications

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

MaxQBiQ8R173.
PaxDbiQ8R173.
PRIDEiQ8R173.

PTM databases

PhosphoSiteiQ8R173.
SwissPalmiQ8R173.

Expressioni

Tissue specificityi

Widely expressed, with highest levels in brain. In the brain, expressed only in neurons (at protein level).3 Publications

Gene expression databases

BgeeiQ8R173.
CleanExiMM_ZDHHC3.
ExpressionAtlasiQ8R173. baseline and differential.
GenevisibleiQ8R173. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8R173. 1 interaction.
STRINGi10090.ENSMUSP00000117392.

Family & Domainsi

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity

Sequence similaritiesi

Belongs to the DHHC palmitoyltransferase family.Curated
Contains 1 DHHC-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri126 – 17651DHHC-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1311. Eukaryota.
COG5273. LUCA.
GeneTreeiENSGT00840000129737.
HOGENOMiHOG000118083.
HOVERGENiHBG056583.
InParanoidiQ8R173.
KOiK18932.
OMAiRAWCVKD.
PhylomeDBiQ8R173.
TreeFamiTF319798.

Family and domain databases

InterProiIPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamiPF01529. zf-DHHC. 1 hit.
[Graphical view]
PROSITEiPS50216. ZF_DHHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R173-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLIPTHHFR DIERKPEYLQ PEKCAPPPFP GPAGAMWFIR DGCGIACAIV
60 70 80 90 100
TWFLVLYAEF VVLFVMLVPS RDYAYSIING IVFNLLAFLA LASHCRAMLT
110 120 130 140 150
DPGAVPKGNA TKEFIESLQL KPGQVVYKCP KCCSIKPDRA HHCSVCKRCI
160 170 180 190 200
RKMDHHCPWV NNCVGENNQK YFVLFTMYIA LISLHALIMV GFHFLHCFEE
210 220 230 240 250
DWTKCSSFSP PTTVILLILL CFEALLFLIF TSVMFGTQVH SICTDETGIE
260 270 280 290
QLKKEERRWA KKTKWMNMKA VFGHPFSLGW ASPFATPDQG KADPYQYVV
Length:299
Mass (Da):34,041
Last modified:June 1, 2002 - v1
Checksum:iBA93F65828A3B36C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti246 – 2461E → G in BAC37939 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB088044 mRNA. Translation: BAC02941.1.
AY166672 mRNA. Translation: AAO27359.1.
AK080541 mRNA. Translation: BAC37939.1.
BC025126 mRNA. Translation: AAH25126.1.
BC052464 mRNA. Translation: AAH52464.1.
CCDSiCCDS23654.1.
RefSeqiNP_081193.2. NM_026917.5.
UniGeneiMm.28300.

Genome annotation databases

EnsembliENSMUST00000147563; ENSMUSP00000117392; ENSMUSG00000025786.
GeneIDi69035.
KEGGimmu:69035.
UCSCiuc009sfq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB088044 mRNA. Translation: BAC02941.1.
AY166672 mRNA. Translation: AAO27359.1.
AK080541 mRNA. Translation: BAC37939.1.
BC025126 mRNA. Translation: AAH25126.1.
BC052464 mRNA. Translation: AAH52464.1.
CCDSiCCDS23654.1.
RefSeqiNP_081193.2. NM_026917.5.
UniGeneiMm.28300.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R173. 1 interaction.
STRINGi10090.ENSMUSP00000117392.

Protein family/group databases

TCDBi9.B.37.2.1. the huntington-interacting protein 14 (hip14) family.

PTM databases

PhosphoSiteiQ8R173.
SwissPalmiQ8R173.

Proteomic databases

MaxQBiQ8R173.
PaxDbiQ8R173.
PRIDEiQ8R173.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000147563; ENSMUSP00000117392; ENSMUSG00000025786.
GeneIDi69035.
KEGGimmu:69035.
UCSCiuc009sfq.2. mouse.

Organism-specific databases

CTDi51304.
MGIiMGI:1926134. Zdhhc3.

Phylogenomic databases

eggNOGiKOG1311. Eukaryota.
COG5273. LUCA.
GeneTreeiENSGT00840000129737.
HOGENOMiHOG000118083.
HOVERGENiHBG056583.
InParanoidiQ8R173.
KOiK18932.
OMAiRAWCVKD.
PhylomeDBiQ8R173.
TreeFamiTF319798.

Enzyme and pathway databases

BRENDAi2.3.1.225. 3474.

Miscellaneous databases

NextBioi328438.
PROiQ8R173.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R173.
CleanExiMM_ZDHHC3.
ExpressionAtlasiQ8R173. baseline and differential.
GenevisibleiQ8R173. MM.

Family and domain databases

InterProiIPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamiPF01529. zf-DHHC. 1 hit.
[Graphical view]
PROSITEiPS50216. ZF_DHHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of Golgi apparatus-specific GODZ with the DHHC zinc finger domain."
    Uemura T., Mori H., Mishina M.
    Biochem. Biophys. Res. Commun. 296:492-496(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Brain.
  2. "The gamma2 subunit of GABA(A) receptors is a substrate for palmitoylation by GODZ."
    Keller C.A., Yuan X., Panzanelli P., Martin M.L., Alldred M., Sassoe-Pognetto M., Luescher B.
    J. Neurosci. 24:5881-5891(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, AUTOPALMITOYLATION, TISSUE SPECIFICITY.
    Strain: BALB/cJ.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Mammary gland.
  5. "Identification of PSD-95 palmitoylating enzymes."
    Fukata M., Fukata Y., Adesnik H., Nicoll R.A., Bredt D.S.
    Neuron 44:987-996(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
  6. "Differential regulation of AMPA receptor subunit trafficking by palmitoylation of two distinct sites."
    Hayashi T., Rumbaugh G., Huganir R.L.
    Neuron 47:709-723(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF CYS-157, AUTOPALMITOYLATION, SUBCELLULAR LOCATION.
  7. "The Golgi S-acylation machinery comprises zDHHC enzymes with major differences in substrate affinity and S-acylation activity."
    Lemonidis K., Gorleku O.A., Sanchez-Perez M.C., Grefen C., Chamberlain L.H.
    Mol. Biol. Cell 25:3870-3883(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, AUTOPALMITOYLATION.

Entry informationi

Entry nameiZDHC3_MOUSE
AccessioniPrimary (citable) accession number: Q8R173
Secondary accession number(s): Q6EMK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: June 1, 2002
Last modified: May 11, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.