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Protein

Valacyclovir hydrolase

Gene

Bphl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine hydrolase that catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. Activates valacyclovir to acyclovir. May play a role in detoxification processes. It is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei139NucleophileBy similarity1
Sitei140Binding of alpha-amino group of substrateBy similarity1
Active sitei244Charge relay systemPROSITE-ProRule annotation1
Active sitei272Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

ReactomeiR-MMU-211945 Phase I - Functionalization of compounds

Protein family/group databases

ESTHERimouse-bphl Valacyclovir-hydrolase
MEROPSiS33.982

Names & Taxonomyi

Protein namesi
Recommended name:
Valacyclovir hydrolase (EC:3.1.-.-)
Short name:
VACVase
Short name:
Valacyclovirase
Alternative name(s):
Biphenyl hydrolase-like protein
Gene namesi
Name:Bphl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1915271 Bphl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37By similarityAdd BLAST37
ChainiPRO_000001784238 – 291Valacyclovir hydrolaseAdd BLAST254

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei74N6-acetyllysine; alternateCombined sources1
Modified residuei74N6-succinyllysine; alternateCombined sources1
Modified residuei86N6-acetyllysineCombined sources1
Modified residuei119N6-acetyllysineCombined sources1
Modified residuei126N6-acetyllysine; alternateCombined sources1
Modified residuei126N6-succinyllysine; alternateCombined sources1
Modified residuei184N6-succinyllysineCombined sources1
Modified residuei191N6-acetyllysine; alternateCombined sources1
Modified residuei191N6-succinyllysine; alternateCombined sources1
Modified residuei217N6-acetyllysineCombined sources1
Modified residuei243N6-acetyllysineCombined sources1
Modified residuei260N6-acetyllysine; alternateCombined sources1
Modified residuei260N6-succinyllysine; alternateCombined sources1
Modified residuei271N6-acetyllysine; alternateCombined sources1
Modified residuei271N6-succinyllysine; alternateCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8R164
MaxQBiQ8R164
PaxDbiQ8R164
PRIDEiQ8R164

PTM databases

iPTMnetiQ8R164
PhosphoSitePlusiQ8R164
SwissPalmiQ8R164

Expressioni

Gene expression databases

BgeeiENSMUSG00000038286
ExpressionAtlasiQ8R164 baseline and differential
GenevisibleiQ8R164 MM

Interactioni

Subunit structurei

Monomer. May also form homodimers (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8R164, 5 interactors
MINTiQ8R164

Structurei

3D structure databases

ProteinModelPortaliQ8R164
SMRiQ8R164
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 173AB hydrolase-1Sequence analysisAdd BLAST112

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2984 Eukaryota
ENOG410YNJD LUCA
GeneTreeiENSGT00390000004746
HOVERGENiHBG048762
InParanoidiQ8R164
KOiK18399
OMAiDGNICRH
OrthoDBiEOG091G0EEA
PhylomeDBiQ8R164
TreeFamiTF318389

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000073 AB_hydrolase_1
PfamiView protein in Pfam
PF00561 Abhydrolase_1, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00120 LIPASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATATVRPAA QRLRLLLSPL KSRICVPQAE PVATFGTAVT SAKVAVNGVH
60 70 80 90 100
LHYQRVGEGE HAILLLPGML GSGKTDFAPQ LQSLNKKRFT LVAWDPRGYG
110 120 130 140 150
YSRPPDRDFP RDFFERDAKD AVDLMKALQF KQVSLLGWSD GGITALIAAA
160 170 180 190 200
KYPSYIRKMV IWGANAYVTE EDSRIYQGIR DVSKWSEKAR KPLEALYGYD
210 220 230 240 250
YLAKTCEDWV DGISQFKQLP EGNICRHLLP LVQCPTLIVH GEKDPLVPRF
260 270 280 290
HADFLLQHVK GSRLHLMPEG KHNLHLRFAD EFNRLVEDFL Q
Length:291
Mass (Da):32,851
Last modified:June 1, 2002 - v1
Checksum:iD3831D8B0674CF7A
GO

Sequence cautioni

The sequence BAC37185 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62A → V in AAH23146 (PubMed:15489334).Curated1
Sequence conflicti218 – 219QL → HC in BAB22447 (PubMed:16141072).Curated2
Sequence conflicti290L → F in BAB22447 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002907 mRNA Translation: BAB22447.1
AK078232 mRNA Translation: BAC37185.1 Different initiation.
BC025162 mRNA Translation: AAH25162.1
BC023146 mRNA Translation: AAH23146.1
CCDSiCCDS49229.1
RefSeqiNP_080788.1, NM_026512.1
UniGeneiMm.140243

Genome annotation databases

EnsembliENSMUST00000040656; ENSMUSP00000046168; ENSMUSG00000038286
GeneIDi68021
KEGGimmu:68021
UCSCiuc007qaz.1 mouse

Similar proteinsi

Entry informationi

Entry nameiBPHL_MOUSE
AccessioniPrimary (citable) accession number: Q8R164
Secondary accession number(s): Q8BVH2, Q8R589, Q9DCC6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health