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Protein

Valacyclovir hydrolase

Gene

Bphl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Serine hydrolase that catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. Activates valacyclovir to acyclovir. May play a role in detoxification processes. It is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei139 – 1391NucleophileBy similarity
Sitei140 – 1401Binding of alpha-amino group of substrateBy similarity
Active sitei244 – 2441Charge relay systemPROSITE-ProRule annotation
Active sitei272 – 2721Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

ReactomeiR-MMU-211945. Phase 1 - Functionalization of compounds.

Protein family/group databases

ESTHERimouse-bphl. Valacyclovir-hydrolase.
MEROPSiS33.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Valacyclovir hydrolase (EC:3.1.-.-)
Short name:
VACVase
Short name:
Valacyclovirase
Alternative name(s):
Biphenyl hydrolase-like protein
Gene namesi
Name:Bphl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1915271. Bphl.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3737By similarityAdd
BLAST
Chaini38 – 291254Valacyclovir hydrolasePRO_0000017842Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei74 – 741N6-acetyllysine; alternateCombined sources
Modified residuei74 – 741N6-succinyllysine; alternateCombined sources
Modified residuei86 – 861N6-acetyllysineCombined sources
Modified residuei119 – 1191N6-acetyllysineCombined sources
Modified residuei126 – 1261N6-acetyllysine; alternateCombined sources
Modified residuei126 – 1261N6-succinyllysine; alternateCombined sources
Modified residuei184 – 1841N6-succinyllysineCombined sources
Modified residuei191 – 1911N6-acetyllysine; alternateCombined sources
Modified residuei191 – 1911N6-succinyllysine; alternateCombined sources
Modified residuei217 – 2171N6-acetyllysineCombined sources
Modified residuei243 – 2431N6-acetyllysineCombined sources
Modified residuei260 – 2601N6-acetyllysine; alternateCombined sources
Modified residuei260 – 2601N6-succinyllysine; alternateCombined sources
Modified residuei271 – 2711N6-acetyllysine; alternateCombined sources
Modified residuei271 – 2711N6-succinyllysine; alternateCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8R164.
MaxQBiQ8R164.
PaxDbiQ8R164.
PRIDEiQ8R164.

PTM databases

iPTMnetiQ8R164.
PhosphoSiteiQ8R164.
SwissPalmiQ8R164.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038286.
GenevisibleiQ8R164. MM.

Interactioni

Subunit structurei

Monomer. May also form homodimers (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8R164. 5 interactions.
MINTiMINT-1860794.
STRINGi10090.ENSMUSP00000046168.

Structurei

3D structure databases

ProteinModelPortaliQ8R164.
SMRiQ8R164. Positions 38-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2984. Eukaryota.
ENOG410YNJD. LUCA.
GeneTreeiENSGT00390000004746.
HOVERGENiHBG048762.
InParanoidiQ8R164.
KOiK18399.
OMAiKWVDGIR.
OrthoDBiEOG091G0EEA.
PhylomeDBiQ8R164.
TreeFamiTF318389.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATATVRPAA QRLRLLLSPL KSRICVPQAE PVATFGTAVT SAKVAVNGVH
60 70 80 90 100
LHYQRVGEGE HAILLLPGML GSGKTDFAPQ LQSLNKKRFT LVAWDPRGYG
110 120 130 140 150
YSRPPDRDFP RDFFERDAKD AVDLMKALQF KQVSLLGWSD GGITALIAAA
160 170 180 190 200
KYPSYIRKMV IWGANAYVTE EDSRIYQGIR DVSKWSEKAR KPLEALYGYD
210 220 230 240 250
YLAKTCEDWV DGISQFKQLP EGNICRHLLP LVQCPTLIVH GEKDPLVPRF
260 270 280 290
HADFLLQHVK GSRLHLMPEG KHNLHLRFAD EFNRLVEDFL Q
Length:291
Mass (Da):32,851
Last modified:June 1, 2002 - v1
Checksum:iD3831D8B0674CF7A
GO

Sequence cautioni

The sequence BAC37185 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621A → V in AAH23146 (PubMed:15489334).Curated
Sequence conflicti218 – 2192QL → HC in BAB22447 (PubMed:16141072).Curated
Sequence conflicti290 – 2901L → F in BAB22447 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002907 mRNA. Translation: BAB22447.1.
AK078232 mRNA. Translation: BAC37185.1. Different initiation.
BC025162 mRNA. Translation: AAH25162.1.
BC023146 mRNA. Translation: AAH23146.1.
CCDSiCCDS49229.1.
RefSeqiNP_080788.1. NM_026512.1.
UniGeneiMm.140243.

Genome annotation databases

EnsembliENSMUST00000040656; ENSMUSP00000046168; ENSMUSG00000038286.
GeneIDi68021.
KEGGimmu:68021.
UCSCiuc007qaz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002907 mRNA. Translation: BAB22447.1.
AK078232 mRNA. Translation: BAC37185.1. Different initiation.
BC025162 mRNA. Translation: AAH25162.1.
BC023146 mRNA. Translation: AAH23146.1.
CCDSiCCDS49229.1.
RefSeqiNP_080788.1. NM_026512.1.
UniGeneiMm.140243.

3D structure databases

ProteinModelPortaliQ8R164.
SMRiQ8R164. Positions 38-291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R164. 5 interactions.
MINTiMINT-1860794.
STRINGi10090.ENSMUSP00000046168.

Protein family/group databases

ESTHERimouse-bphl. Valacyclovir-hydrolase.
MEROPSiS33.982.

PTM databases

iPTMnetiQ8R164.
PhosphoSiteiQ8R164.
SwissPalmiQ8R164.

Proteomic databases

EPDiQ8R164.
MaxQBiQ8R164.
PaxDbiQ8R164.
PRIDEiQ8R164.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040656; ENSMUSP00000046168; ENSMUSG00000038286.
GeneIDi68021.
KEGGimmu:68021.
UCSCiuc007qaz.1. mouse.

Organism-specific databases

CTDi670.
MGIiMGI:1915271. Bphl.

Phylogenomic databases

eggNOGiKOG2984. Eukaryota.
ENOG410YNJD. LUCA.
GeneTreeiENSGT00390000004746.
HOVERGENiHBG048762.
InParanoidiQ8R164.
KOiK18399.
OMAiKWVDGIR.
OrthoDBiEOG091G0EEA.
PhylomeDBiQ8R164.
TreeFamiTF318389.

Enzyme and pathway databases

ReactomeiR-MMU-211945. Phase 1 - Functionalization of compounds.

Miscellaneous databases

PROiQ8R164.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038286.
GenevisibleiQ8R164. MM.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBPHL_MOUSE
AccessioniPrimary (citable) accession number: Q8R164
Secondary accession number(s): Q8BVH2, Q8R589, Q9DCC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.