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Q8R146

- APEH_MOUSE

UniProt

Q8R146 - APEH_MOUSE

Protein

Acylamino-acid-releasing enzyme

Gene

Apeh

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 3 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser By similarity.By similarity

    Catalytic activityi

    Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei587 – 5871Charge relay systemPROSITE-ProRule annotation
    Active sitei675 – 6751Charge relay systemPROSITE-ProRule annotation
    Active sitei707 – 7071Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Protein family/group databases

    MEROPSiS09.004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acylamino-acid-releasing enzyme (EC:3.4.19.1)
    Short name:
    AARE
    Alternative name(s):
    Acyl-peptide hydrolase
    Short name:
    APH
    Acylaminoacyl-peptidase
    Gene namesi
    Name:Apeh
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:88041. Apeh.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nuclear membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 732732Acylamino-acid-releasing enzymePRO_0000122431Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei185 – 1851PhosphoserineBy similarity
    Modified residuei187 – 1871PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8R146.
    PaxDbiQ8R146.
    PRIDEiQ8R146.

    PTM databases

    PhosphoSiteiQ8R146.

    Expressioni

    Gene expression databases

    CleanExiMM_APEH.
    GenevestigatoriQ8R146.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    IntActiQ8R146. 1 interaction.
    MINTiMINT-1856385.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R146.
    SMRiQ8R146. Positions 375-729.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S9C family.Curated

    Phylogenomic databases

    eggNOGiCOG1506.
    GeneTreeiENSGT00390000013172.
    HOGENOMiHOG000007443.
    HOVERGENiHBG000869.
    InParanoidiQ8R146.
    KOiK01303.
    OMAiPEQENVQ.
    OrthoDBiEOG776SPB.
    TreeFamiTF312937.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR002471. Pept_S9_AS.
    IPR001375. Peptidase_S9.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view]
    PfamiPF00326. Peptidase_S9. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8R146-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MERQVLLSEP QEAAALYRGL SRQPSLSAAC LGPEVTTQYG GLYRTVHTEW    50
    TQRDLDRMEN IRFCRQYLVF HDGDSVVFAG PAGNSVETRG ELLSRESPSG 100
    TMKAVLRKAG GAVSGEEKQF LEVWEKNRKL KSFNLSALEK HGPVYEDDCF 150
    GCLSWSHSET HLLYVAEKKR PKAESFFQTK ALDVSASDEE MARPKKPDQA 200
    IKGDQFVFYE DWGETMVSKS IPVLCVLDIE SGNISVLEGV PENVSPGQAF 250
    WAPGDTGVVF VGWWHEPFRL GIRYCTNRRS ALYYVDLSGG KCELLSDESL 300
    AVCSPRLSPD QCRVVYLQYP SLAPHHQCSQ LFLYDWYTKV TSLVVDIVPR 350
    QLGESFSGIY CSLLPLGCWS ADSQRVVFDS VQRSRQDLFA VDTQTGSVTS 400
    LTAGGSAGSW KLLTIDRDLM VAQFSTPNLP PSLKVGFLPP AGKEQSVSWV 450
    SLEEAEPIPD IHWGIRVLHP PPDQENVQYA DLDFEAILLQ PSNSPDKSQV 500
    PMVVMPHGGP HSSFVTAWML FPAMLCKMGF AVLLVNYRGS TGFGQDSILS 550
    LPGNVGHQDV KDVQFAVQQV LQEEHFDARR VALMGGSHGG FLSCHLIGQY 600
    PETYSACIAR NPVINIVSMM GTTDIPDWCM VETGFPYSND YLPDLNVLEE 650
    MLDKSPIKYI PQVKTPVLLM LGQEDRRVPF KQGLEYYHAL KARNVPVRLL 700
    LYPKSTHALS EVEVESDSFM NTVLWLHTHL GS 732
    Length:732
    Mass (Da):81,581
    Last modified:October 3, 2012 - v3
    Checksum:i899321A91EF39AD3
    GO
    Isoform 2 (identifier: Q8R146-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         508-522: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:717
    Mass (Da):79,965
    Checksum:iC60BC2F986EDEC9C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti89 – 891R → P in AAH25494. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei508 – 52215Missing in isoform 2. 1 PublicationVSP_008866Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC137678 Genomic DNA. No translation available.
    CH466560 Genomic DNA. Translation: EDL21266.1.
    BC025494 mRNA. Translation: AAH25494.1.
    BC026594 mRNA. Translation: AAH26594.1.
    BC034199 mRNA. Translation: AAH34199.1.
    CCDSiCCDS52923.1. [Q8R146-1]
    RefSeqiNP_666338.2. NM_146226.2. [Q8R146-1]
    UniGeneiMm.219639.

    Genome annotation databases

    EnsembliENSMUST00000081309; ENSMUSP00000080058; ENSMUSG00000032590. [Q8R146-1]
    GeneIDi235606.
    KEGGimmu:235606.
    UCSCiuc009ros.2. mouse. [Q8R146-2]
    uc009rot.2. mouse.
    uc009rou.2. mouse. [Q8R146-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC137678 Genomic DNA. No translation available.
    CH466560 Genomic DNA. Translation: EDL21266.1 .
    BC025494 mRNA. Translation: AAH25494.1 .
    BC026594 mRNA. Translation: AAH26594.1 .
    BC034199 mRNA. Translation: AAH34199.1 .
    CCDSi CCDS52923.1. [Q8R146-1 ]
    RefSeqi NP_666338.2. NM_146226.2. [Q8R146-1 ]
    UniGenei Mm.219639.

    3D structure databases

    ProteinModelPortali Q8R146.
    SMRi Q8R146. Positions 375-729.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8R146. 1 interaction.
    MINTi MINT-1856385.

    Chemistry

    ChEMBLi CHEMBL2189142.

    Protein family/group databases

    MEROPSi S09.004.

    PTM databases

    PhosphoSitei Q8R146.

    Proteomic databases

    MaxQBi Q8R146.
    PaxDbi Q8R146.
    PRIDEi Q8R146.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000081309 ; ENSMUSP00000080058 ; ENSMUSG00000032590 . [Q8R146-1 ]
    GeneIDi 235606.
    KEGGi mmu:235606.
    UCSCi uc009ros.2. mouse. [Q8R146-2 ]
    uc009rot.2. mouse.
    uc009rou.2. mouse. [Q8R146-1 ]

    Organism-specific databases

    CTDi 327.
    MGIi MGI:88041. Apeh.

    Phylogenomic databases

    eggNOGi COG1506.
    GeneTreei ENSGT00390000013172.
    HOGENOMi HOG000007443.
    HOVERGENi HBG000869.
    InParanoidi Q8R146.
    KOi K01303.
    OMAi PEQENVQ.
    OrthoDBi EOG776SPB.
    TreeFami TF312937.

    Miscellaneous databases

    ChiTaRSi APEH. mouse.
    NextBioi 382781.
    PROi Q8R146.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_APEH.
    Genevestigatori Q8R146.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR002471. Pept_S9_AS.
    IPR001375. Peptidase_S9.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view ]
    Pfami PF00326. Peptidase_S9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00708. PRO_ENDOPEP_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Colon, Kidney and Liver.

    Entry informationi

    Entry nameiAPEH_MOUSE
    AccessioniPrimary (citable) accession number: Q8R146
    Secondary accession number(s): G3X9I2, Q8K029, Q8R0M9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 7, 2003
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 97 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3