Reviewed, UniProtKB/Swiss-Prot Q8R146 (APEH_MOUSE)

Names and origin

Protein names

Recommended name:
    Acylamino-acid-releasing enzyme
      Short name=AARE
    EC=3.4.19.1
Alternative name(s):
    Acyl-peptide hydrolase
      Short name=APH
    Acylaminoacyl-peptidase

Gene names

Name:

Apeh

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser By similarity.
Catalytic activity	Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase S9C family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase
PTM	Acetylation
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8R146-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8R146-2)

The sequence of this isoform differs from the canonical sequence as follows:
     508-522: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 732	732	Acylamino-acid-releasing enzyme		PRO_0000122431
Sites
	Active site	587	1	Charge relay system By similarity
	Active site	675	1	Charge relay system By similarity
	Active site	707	1	Charge relay system By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
Natural variations
	Alternative sequence	508 – 522	15	Missing in isoform 2.		VSP_008866

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 7, 2003. Version 2. Checksum: D393E9F600BB2CC4	FASTA	732	81,522
	10 20 30 40 50 60 MERQVLLSEP QEAAALYRGL SRQPSLSAAC LGPEVTTQYG GLYRTVHTEW TQRDLDRMEN 70 80 90 100 110 120 IRFCRQYLVF HDGDSVVFAG PAGNSVETPG ELLSRESPSG TMKAVLRKAG GAVSGEEKQF 130 140 150 160 170 180 LEVWEKNRKL KSFNLSALEK HGPVYEDDCF GCLSWSHSET HLLYVAEKKR PKAESFFQTK 190 200 210 220 230 240 ALDVSASDEE MARPKKPDQA IKGDQFVFYE DWGETMVSKS IPVLCVLDIE SGNISVLEGV 250 260 270 280 290 300 PENVSPGQAF WAPGDTGVVF VGWWHEPFRL GIRYCTNRRS ALYYVDLSGG KCELLSDESL 310 320 330 340 350 360 AVCSPRLSPD QCRVVYLQYP SLAPHHQCSQ LFLYDWYTKV TSLVVDIVPR QLGESFSGIY 370 380 390 400 410 420 CSLLPLGCWS ADSQRVVFDS VQRSRQDLFA VDTQTGSVTS LTAGGSAGSW KLLTIDRDLM 430 440 450 460 470 480 VAQFSTPNLP PSLKVGFLPP AGKEQSVSWV SLEEAEPIPD IHWGIRVLHP PPDQENVQYA 490 500 510 520 530 540 DLDFEAILLQ PSNSPDKSQV PMVVMPHGGP HSSFVTAWML FPAMLCKMGF AVLLVNYRGS 550 560 570 580 590 600 TGFGQDSILS LPGNVGHQDV KDVQFAVQQV LQEEHFDARR VALMGGSHGG FLSCHLIGQY 610 620 630 640 650 660 PETYSACIAR NPVINIVSMM GTTDIPDWCM VETGFPYSND YLPDLNVLEE MLDKSPIKYI 670 680 690 700 710 720 PQVKTPVLLM LGQEDRRVPF KQGLEYYHAL KARNVPVRLL LYPKSTHALS EVEVESDSFM 730 NTVLWLHTHL GS
	Isoform 2. Checksum: B40BC20F44A9F6CD	FASTA	717	79,906
	10 20 30 40 50 60 MERQVLLSEP QEAAALYRGL SRQPSLSAAC LGPEVTTQYG GLYRTVHTEW TQRDLDRMEN 70 80 90 100 110 120 IRFCRQYLVF HDGDSVVFAG PAGNSVETPG ELLSRESPSG TMKAVLRKAG GAVSGEEKQF 130 140 150 160 170 180 LEVWEKNRKL KSFNLSALEK HGPVYEDDCF GCLSWSHSET HLLYVAEKKR PKAESFFQTK 190 200 210 220 230 240 ALDVSASDEE MARPKKPDQA IKGDQFVFYE DWGETMVSKS IPVLCVLDIE SGNISVLEGV 250 260 270 280 290 300 PENVSPGQAF WAPGDTGVVF VGWWHEPFRL GIRYCTNRRS ALYYVDLSGG KCELLSDESL 310 320 330 340 350 360 AVCSPRLSPD QCRVVYLQYP SLAPHHQCSQ LFLYDWYTKV TSLVVDIVPR QLGESFSGIY 370 380 390 400 410 420 CSLLPLGCWS ADSQRVVFDS VQRSRQDLFA VDTQTGSVTS LTAGGSAGSW KLLTIDRDLM 430 440 450 460 470 480 VAQFSTPNLP PSLKVGFLPP AGKEQSVSWV SLEEAEPIPD IHWGIRVLHP PPDQENVQYA 490 500 510 520 530 540 DLDFEAILLQ PSNSPDKSQV PMVVMPHAML CKMGFAVLLV NYRGSTGFGQ DSILSLPGNV 550 560 570 580 590 600 GHQDVKDVQF AVQQVLQEEH FDARRVALMG GSHGGFLSCH LIGQYPETYS ACIARNPVIN 610 620 630 640 650 660 IVSMMGTTDI PDWCMVETGF PYSNDYLPDL NVLEEMLDKS PIKYIPQVKT PVLLMLGQED 670 680 690 700 710 RRVPFKQGLE YYHALKARNV PVRLLLYPKS THALSEVEVE SDSFMNTVLW LHTHLGS

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Colon, Kidney and Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC025494 mRNA. Translation: AAH25494.1. BC026594 mRNA. Translation: AAH26594.1. BC034199 mRNA. Translation: AAH34199.1.
IPI	IPI00279218. IPI00387245.
UniGene	Mm.219639
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8R146.
Protein family/group databases
MEROPS	S09.004.
PTM databases
PhosphoSite	Q8R146.
Proteomic databases
PRIDE	Q8R146.
Genome annotation databases
Ensembl	ENSMUST00000081309; ENSMUSP00000080058; ENSMUSG00000032590; Mus musculus. [Genome view]
UCSC	uc009ros.1. mouse. uc009rot.1. mouse.
Organism-specific databases
MGI	MGI:88041. Apeh.
Phylogenomic databases
HOGENOM	Q8R146.
HOVERGEN	Q8R146.
Enzyme and pathway databases
BRENDA	3.4.19.1. 244.
Gene expression databases
ArrayExpress	Q8R146.
Bgee	Q8R146.
CleanEx	MM_APEH.
Genevestigator	Q8R146.
GermOnline	ENSMUSG00000032590. Mus musculus.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR002471. Pept_S9_AS. IPR001375. Peptidase_S9. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
Pfam	PF00326. Peptidase_S9. 1 hit. [Graphical view]
PROSITE	PS00708. PRO_ENDOPEP_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	382781.
SOURCE	Search...

Entry information

Entry name

APEH_MOUSE

Accession

Primary (citable) accession number: Q8R146
Secondary accession number(s): Q8K029, Q8R0M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	November 7, 2003
Last modified:	October 13, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)