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Protein

Acylamino-acid-releasing enzyme

Gene

Apeh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser (By similarity).By similarity

Catalytic activityi

Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei587 – 5871Charge relay systemPROSITE-ProRule annotation
Active sitei675 – 6751Charge relay systemPROSITE-ProRule annotation
Active sitei707 – 7071Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Protein family/group databases

ESTHERimouse-apeh. ACPH_Peptidase_S9.
MEROPSiS09.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Acylamino-acid-releasing enzyme (EC:3.4.19.1)
Short name:
AARE
Alternative name(s):
Acyl-peptide hydrolase
Short name:
APH
Acylaminoacyl-peptidase
Gene namesi
Name:Apeh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:88041. Apeh.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 732732Acylamino-acid-releasing enzymePRO_0000122431Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei185 – 1851PhosphoserineBy similarity
Modified residuei187 – 1871PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8R146.
PaxDbiQ8R146.
PRIDEiQ8R146.

PTM databases

PhosphoSiteiQ8R146.

Expressioni

Gene expression databases

CleanExiMM_APEH.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiQ8R146. 1 interaction.
MINTiMINT-1856385.
STRINGi10090.ENSMUSP00000080058.

Structurei

3D structure databases

ProteinModelPortaliQ8R146.
SMRiQ8R146. Positions 375-729.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9C family.Curated

Phylogenomic databases

eggNOGiCOG1506.
GeneTreeiENSGT00390000013172.
HOGENOMiHOG000007443.
HOVERGENiHBG000869.
InParanoidiQ8R146.
KOiK01303.
OMAiIGGKCEL.
OrthoDBiEOG776SPB.
TreeFamiTF312937.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR001375. Peptidase_S9.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R146-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERQVLLSEP QEAAALYRGL SRQPSLSAAC LGPEVTTQYG GLYRTVHTEW
60 70 80 90 100
TQRDLDRMEN IRFCRQYLVF HDGDSVVFAG PAGNSVETRG ELLSRESPSG
110 120 130 140 150
TMKAVLRKAG GAVSGEEKQF LEVWEKNRKL KSFNLSALEK HGPVYEDDCF
160 170 180 190 200
GCLSWSHSET HLLYVAEKKR PKAESFFQTK ALDVSASDEE MARPKKPDQA
210 220 230 240 250
IKGDQFVFYE DWGETMVSKS IPVLCVLDIE SGNISVLEGV PENVSPGQAF
260 270 280 290 300
WAPGDTGVVF VGWWHEPFRL GIRYCTNRRS ALYYVDLSGG KCELLSDESL
310 320 330 340 350
AVCSPRLSPD QCRVVYLQYP SLAPHHQCSQ LFLYDWYTKV TSLVVDIVPR
360 370 380 390 400
QLGESFSGIY CSLLPLGCWS ADSQRVVFDS VQRSRQDLFA VDTQTGSVTS
410 420 430 440 450
LTAGGSAGSW KLLTIDRDLM VAQFSTPNLP PSLKVGFLPP AGKEQSVSWV
460 470 480 490 500
SLEEAEPIPD IHWGIRVLHP PPDQENVQYA DLDFEAILLQ PSNSPDKSQV
510 520 530 540 550
PMVVMPHGGP HSSFVTAWML FPAMLCKMGF AVLLVNYRGS TGFGQDSILS
560 570 580 590 600
LPGNVGHQDV KDVQFAVQQV LQEEHFDARR VALMGGSHGG FLSCHLIGQY
610 620 630 640 650
PETYSACIAR NPVINIVSMM GTTDIPDWCM VETGFPYSND YLPDLNVLEE
660 670 680 690 700
MLDKSPIKYI PQVKTPVLLM LGQEDRRVPF KQGLEYYHAL KARNVPVRLL
710 720 730
LYPKSTHALS EVEVESDSFM NTVLWLHTHL GS
Length:732
Mass (Da):81,581
Last modified:October 3, 2012 - v3
Checksum:i899321A91EF39AD3
GO
Isoform 2 (identifier: Q8R146-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     508-522: Missing.

Note: No experimental confirmation available.
Show »
Length:717
Mass (Da):79,965
Checksum:iC60BC2F986EDEC9C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891R → P in AAH25494 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei508 – 52215Missing in isoform 2. 1 PublicationVSP_008866Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC137678 Genomic DNA. No translation available.
CH466560 Genomic DNA. Translation: EDL21266.1.
BC025494 mRNA. Translation: AAH25494.1.
BC026594 mRNA. Translation: AAH26594.1.
BC034199 mRNA. Translation: AAH34199.1.
CCDSiCCDS52923.1. [Q8R146-1]
RefSeqiNP_666338.2. NM_146226.2. [Q8R146-1]
UniGeneiMm.219639.

Genome annotation databases

EnsembliENSMUST00000193254; ENSMUSP00000141856; ENSMUSG00000032590. [Q8R146-1]
GeneIDi235606.
KEGGimmu:235606.
UCSCiuc009ros.2. mouse. [Q8R146-2]
uc009rot.2. mouse.
uc009rou.2. mouse. [Q8R146-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC137678 Genomic DNA. No translation available.
CH466560 Genomic DNA. Translation: EDL21266.1.
BC025494 mRNA. Translation: AAH25494.1.
BC026594 mRNA. Translation: AAH26594.1.
BC034199 mRNA. Translation: AAH34199.1.
CCDSiCCDS52923.1. [Q8R146-1]
RefSeqiNP_666338.2. NM_146226.2. [Q8R146-1]
UniGeneiMm.219639.

3D structure databases

ProteinModelPortaliQ8R146.
SMRiQ8R146. Positions 375-729.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R146. 1 interaction.
MINTiMINT-1856385.
STRINGi10090.ENSMUSP00000080058.

Chemistry

ChEMBLiCHEMBL2189142.

Protein family/group databases

ESTHERimouse-apeh. ACPH_Peptidase_S9.
MEROPSiS09.004.

PTM databases

PhosphoSiteiQ8R146.

Proteomic databases

MaxQBiQ8R146.
PaxDbiQ8R146.
PRIDEiQ8R146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000193254; ENSMUSP00000141856; ENSMUSG00000032590. [Q8R146-1]
GeneIDi235606.
KEGGimmu:235606.
UCSCiuc009ros.2. mouse. [Q8R146-2]
uc009rot.2. mouse.
uc009rou.2. mouse. [Q8R146-1]

Organism-specific databases

CTDi327.
MGIiMGI:88041. Apeh.

Phylogenomic databases

eggNOGiCOG1506.
GeneTreeiENSGT00390000013172.
HOGENOMiHOG000007443.
HOVERGENiHBG000869.
InParanoidiQ8R146.
KOiK01303.
OMAiIGGKCEL.
OrthoDBiEOG776SPB.
TreeFamiTF312937.

Miscellaneous databases

ChiTaRSiApeh. mouse.
NextBioi382781.
PROiQ8R146.
SOURCEiSearch...

Gene expression databases

CleanExiMM_APEH.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR001375. Peptidase_S9.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Colon, Kidney and Liver.

Entry informationi

Entry nameiAPEH_MOUSE
AccessioniPrimary (citable) accession number: Q8R146
Secondary accession number(s): G3X9I2, Q8K029, Q8R0M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: October 3, 2012
Last modified: June 24, 2015
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.