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Q8R139 (S29A4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Equilibrative nucleoside transporter 4
Alternative name(s):
Solute carrier family 29 member 4
Gene names
Name:Slc29a4
Synonyms:Ent4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length528 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a polyspecific organic cation transporter, efficiently transporting many organic cations such as monoamine neurotransmitters 1-methyl-4-phenylpyridinium and biogenic amines including serotonin, dopamine, norepinephrine and epinephrine. May play a role in regulating central nervous system homeostasis of monoamine neurotransmitters. May be involved in luminal transport of organic cations in the kidney and seems to use luminal proton gradient to drive organic cation reabsorptprev reabsorption. Does not seem to transport nucleoside and nucleoside analogs such as uridine, cytidine, thymidine, adenosine, inosine, guanosine, and azidothymidine. In (Ref.2) adenosine is efficiently transported but in a fashion highly sensitive to extracellular pH, with maximal activity in the pH range 5.5 to 6.5. Glu-206 is essential for the cation selectivity and may function as the charge sensor for cationic substrates. Transport is chloride and sodium-independent but appears to be sensitive to changes in membrane potential. Weakly inhibited by the classical inhibitors of equilibrative nucleoside transport, dipyridamole, dilazep, and nitrobenzylthioinosine. May play a role in the regulation of extracellular adenosine concentrations in cardiac tissues, in particular during ischemia By similarity. Ref.2

Subcellular location

Cell membrane; Multi-pass membrane protein Potential. Apical cell membrane; Multi-pass membrane protein By similarity. Note: Located to the plasma membranes of ventricular myocytes and vascular endothelial cells. Targeted to the apical membranes of differentiated kidney epithelial cells By similarity.

Tissue specificity

Expressed in heart. Ref.2

Post-translational modification

N-glycosylated. Ref.2

Sequence similarities

Belongs to the SLC29A/ENT transporter (TC 2.A.57) family. [View classification]

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentapical plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionnucleoside transmembrane transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 528528Equilibrative nucleoside transporter 4
PRO_0000326252

Regions

Topological domain1 – 6868Extracellular Potential
Transmembrane69 – 8921Helical; Potential
Topological domain90 – 10112Cytoplasmic Potential
Transmembrane102 – 12221Helical; Potential
Topological domain123 – 13917Extracellular Potential
Transmembrane140 – 16021Helical; Potential
Topological domain161 – 1666Cytoplasmic Potential
Transmembrane167 – 18721Helical; Potential
Topological domain188 – 23144Extracellular Potential
Transmembrane232 – 25221Helical; Potential
Topological domain253 – 34694Cytoplasmic Potential
Transmembrane347 – 36721Helical; Potential
Topological domain368 – 3769Extracellular Potential
Transmembrane377 – 39721Helical; Potential
Topological domain398 – 41114Cytoplasmic Potential
Transmembrane412 – 43221Helical; Potential
Topological domain433 – 44513Extracellular Potential
Transmembrane446 – 46621Helical; Potential
Topological domain467 – 48115Cytoplasmic Potential
Transmembrane482 – 50423Helical; Potential
Topological domain505 – 52824Extracellular Potential
Compositional bias515 – 5206Poly-Ala

Amino acid modifications

Glycosylation5211N-linked (GlcNAc...) Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8R139 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 96379B4FBB2AD2ED

FASTA52858,099
        10         20         30         40         50         60 
MGSIGSQRLK EPCVAATSDQ SVVTSFSFDN FQLETTAEGA QDPGIRVRGV PTFTDSAVEE 

        70         80         90        100        110        120 
PVPDDRYHAI YFAMLLAGVG FLLPYNSFIT DVDYLHHKYP GTSIVFDMSL TYILVALAAV 

       130        140        150        160        170        180 
LLNNVVVERL NLHTRITTGY LLALGPLLFI SICDVWLQLF SHDQAYAINL AAVGTVAFGC 

       190        200        210        220        230        240 
TVQQSSFYGY TGLLPKRYTQ GVMTGESTAG VMISLSRILT KLLLPDERAS TIIFFLVSAG 

       250        260        270        280        290        300 
LELLCFLLHL LVRRSRFVLY YTTRPRDSRP VQAGYRVHHD VASGDIHFEH QTPALSSSRS 

       310        320        330        340        350        360 
PKESPAHEVT HSNSGVYMRF DVPRPRVKRS WPTFRALLLH RYVVARVIWA DMLSIAVTYF 

       370        380        390        400        410        420 
ITLCLFPGLE SEIRHCVLGE WLPILVMAVF NLSDFVGKIL AALPVEWRGT HLLACSCLRV 

       430        440        450        460        470        480 
VFIPLFILCV YPSGMPALRH PAWPCVFSLL MGISNGYFGS VPMILAAGKV SPKQRELAGN 

       490        500        510        520 
TMTVSYMSGL TLGSAVAYCT YSLTRDAHGS CFQTATAAAA NDSIPVGP 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary tumor.
[2]"Distribution and functional characterization of equilibrative nucleoside transporter-4, a novel cardiac adenosine transporter activated at acidic pH."
Barnes K., Dobrzynski H., Foppolo S., Beal P.R., Ismat F., Scullion E.R., Sun L., Tellez J., Ritzel M.W., Claycomb W.C., Cass C.E., Young J.D., Billeter-Clark R., Boyett M.R., Baldwin S.A.
Circ. Res. 99:510-519(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, GLYCOSYLATION AT ASN-521.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC025599 mRNA. Translation: AAH25599.1.
RefSeqNP_666369.1. NM_146257.2.
XP_006504769.1. XM_006504706.1.
UniGeneMm.125942.

3D structure databases

ProteinModelPortalQ8R139.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8R139.

Proteomic databases

PRIDEQ8R139.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000058418; ENSMUSP00000059896; ENSMUSG00000050822.
GeneID243328.
KEGGmmu:243328.
UCSCuc009aiz.1. mouse.

Organism-specific databases

CTD222962.
MGIMGI:2385330. Slc29a4.

Phylogenomic databases

eggNOGNOG249415.
GeneTreeENSGT00390000002232.
HOGENOMHOG000048076.
HOVERGENHBG095787.
InParanoidQ8R139.
KOK03323.
OMAASTIIFF.
OrthoDBEOG73Z2T0.
PhylomeDBQ8R139.
TreeFamTF313950.

Gene expression databases

BgeeQ8R139.
GenevestigatorQ8R139.

Family and domain databases

InterProIPR002259. Eqnu_transpt.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PANTHERPTHR10332. PTHR10332. 1 hit.
PfamPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PRINTSPR01130. DERENTRNSPRT.
SUPFAMSSF103473. SSF103473. 2 hits.
ProtoNetSearch...

Other

NextBio385721.
PROQ8R139.
SOURCESearch...

Entry information

Entry nameS29A4_MOUSE
AccessionPrimary (citable) accession number: Q8R139
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot