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Protein

Equilibrative nucleoside transporter 4

Gene

Slc29a4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a polyspecific organic cation transporter, efficiently transporting many organic cations such as monoamine neurotransmitters 1-methyl-4-phenylpyridinium and biogenic amines including serotonin, dopamine, norepinephrine and epinephrine. May play a role in regulating central nervous system homeostasis of monoamine neurotransmitters. May be involved in luminal transport of organic cations in the kidney and seems to use luminal proton gradient to drive organic cation reabsorptprev reabsorption. Does not seem to transport nucleoside and nucleoside analogs such as uridine, cytidine, thymidine, adenosine, inosine, guanosine, and azidothymidine. In (PubMed:16873718) adenosine is efficiently transported but in a fashion highly sensitive to extracellular pH, with maximal activity in the pH range 5.5 to 6.5. Glu-206 is essential for the cation selectivity and may function as the charge sensor for cationic substrates. Transport is chloride and sodium-independent but appears to be sensitive to changes in membrane potential. Weakly inhibited by the classical inhibitors of equilibrative nucleoside transport, dipyridamole, dilazep, and nitrobenzylthioinosine. May play a role in the regulation of extracellular adenosine concentrations in cardiac tissues, in particular during ischemia (By similarity).By similarity1 Publication

GO - Molecular functioni

  1. monoamine transmembrane transporter activity Source: Ensembl
  2. nucleoside transmembrane transporter activity Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_339322. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Equilibrative nucleoside transporter 4
Alternative name(s):
Solute carrier family 29 member 4
Gene namesi
Name:Slc29a4
Synonyms:Ent4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2385330. Slc29a4.

Subcellular locationi

  1. Cell membrane Curated; Multi-pass membrane protein Curated
  2. Apical cell membrane By similarity; Multi-pass membrane protein By similarity

  3. Note: Located to the plasma membranes of ventricular myocytes and vascular endothelial cells. Targeted to the apical membranes of differentiated kidney epithelial cells (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6868ExtracellularSequence AnalysisAdd
BLAST
Transmembranei69 – 8921HelicalSequence AnalysisAdd
BLAST
Topological domaini90 – 10112CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei102 – 12221HelicalSequence AnalysisAdd
BLAST
Topological domaini123 – 13917ExtracellularSequence AnalysisAdd
BLAST
Transmembranei140 – 16021HelicalSequence AnalysisAdd
BLAST
Topological domaini161 – 1666CytoplasmicSequence Analysis
Transmembranei167 – 18721HelicalSequence AnalysisAdd
BLAST
Topological domaini188 – 23144ExtracellularSequence AnalysisAdd
BLAST
Transmembranei232 – 25221HelicalSequence AnalysisAdd
BLAST
Topological domaini253 – 34694CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei347 – 36721HelicalSequence AnalysisAdd
BLAST
Topological domaini368 – 3769ExtracellularSequence Analysis
Transmembranei377 – 39721HelicalSequence AnalysisAdd
BLAST
Topological domaini398 – 41114CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei412 – 43221HelicalSequence AnalysisAdd
BLAST
Topological domaini433 – 44513ExtracellularSequence AnalysisAdd
BLAST
Transmembranei446 – 46621HelicalSequence AnalysisAdd
BLAST
Topological domaini467 – 48115CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei482 – 50423HelicalSequence AnalysisAdd
BLAST
Topological domaini505 – 52824ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 528528Equilibrative nucleoside transporter 4PRO_0000326252Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi521 – 5211N-linked (GlcNAc...)1 Publication

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ8R139.

PTM databases

PhosphoSiteiQ8R139.

Expressioni

Tissue specificityi

Expressed in heart.1 Publication

Gene expression databases

BgeeiQ8R139.
GenevestigatoriQ8R139.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi515 – 5206Poly-Ala

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249415.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000048076.
HOVERGENiHBG095787.
InParanoidiQ8R139.
KOiK03323.
OMAiRELAXSA.
OrthoDBiEOG73Z2T0.
PhylomeDBiQ8R139.
TreeFamiTF313950.

Family and domain databases

InterProiIPR030198. ENT4.
IPR002259. Eqnu_transpt.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 1 hit.
PTHR10332:SF4. PTHR10332:SF4. 1 hit.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8R139-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSIGSQRLK EPCVAATSDQ SVVTSFSFDN FQLETTAEGA QDPGIRVRGV
60 70 80 90 100
PTFTDSAVEE PVPDDRYHAI YFAMLLAGVG FLLPYNSFIT DVDYLHHKYP
110 120 130 140 150
GTSIVFDMSL TYILVALAAV LLNNVVVERL NLHTRITTGY LLALGPLLFI
160 170 180 190 200
SICDVWLQLF SHDQAYAINL AAVGTVAFGC TVQQSSFYGY TGLLPKRYTQ
210 220 230 240 250
GVMTGESTAG VMISLSRILT KLLLPDERAS TIIFFLVSAG LELLCFLLHL
260 270 280 290 300
LVRRSRFVLY YTTRPRDSRP VQAGYRVHHD VASGDIHFEH QTPALSSSRS
310 320 330 340 350
PKESPAHEVT HSNSGVYMRF DVPRPRVKRS WPTFRALLLH RYVVARVIWA
360 370 380 390 400
DMLSIAVTYF ITLCLFPGLE SEIRHCVLGE WLPILVMAVF NLSDFVGKIL
410 420 430 440 450
AALPVEWRGT HLLACSCLRV VFIPLFILCV YPSGMPALRH PAWPCVFSLL
460 470 480 490 500
MGISNGYFGS VPMILAAGKV SPKQRELAGN TMTVSYMSGL TLGSAVAYCT
510 520
YSLTRDAHGS CFQTATAAAA NDSIPVGP
Length:528
Mass (Da):58,099
Last modified:June 1, 2002 - v1
Checksum:i96379B4FBB2AD2ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC025599 mRNA. Translation: AAH25599.1.
CCDSiCCDS39364.1.
RefSeqiNP_666369.1. NM_146257.2.
XP_006504769.1. XM_006504706.1.
UniGeneiMm.125942.

Genome annotation databases

EnsembliENSMUST00000058418; ENSMUSP00000059896; ENSMUSG00000050822.
GeneIDi243328.
KEGGimmu:243328.
UCSCiuc009aiz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC025599 mRNA. Translation: AAH25599.1.
CCDSiCCDS39364.1.
RefSeqiNP_666369.1. NM_146257.2.
XP_006504769.1. XM_006504706.1.
UniGeneiMm.125942.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8R139.

Proteomic databases

PRIDEiQ8R139.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058418; ENSMUSP00000059896; ENSMUSG00000050822.
GeneIDi243328.
KEGGimmu:243328.
UCSCiuc009aiz.1. mouse.

Organism-specific databases

CTDi222962.
MGIiMGI:2385330. Slc29a4.

Phylogenomic databases

eggNOGiNOG249415.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000048076.
HOVERGENiHBG095787.
InParanoidiQ8R139.
KOiK03323.
OMAiRELAXSA.
OrthoDBiEOG73Z2T0.
PhylomeDBiQ8R139.
TreeFamiTF313950.

Enzyme and pathway databases

ReactomeiREACT_339322. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Miscellaneous databases

NextBioi385721.
PROiQ8R139.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R139.
GenevestigatoriQ8R139.

Family and domain databases

InterProiIPR030198. ENT4.
IPR002259. Eqnu_transpt.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 1 hit.
PTHR10332:SF4. PTHR10332:SF4. 1 hit.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  2. "Distribution and functional characterization of equilibrative nucleoside transporter-4, a novel cardiac adenosine transporter activated at acidic pH."
    Barnes K., Dobrzynski H., Foppolo S., Beal P.R., Ismat F., Scullion E.R., Sun L., Tellez J., Ritzel M.W., Claycomb W.C., Cass C.E., Young J.D., Billeter-Clark R., Boyett M.R., Baldwin S.A.
    Circ. Res. 99:510-519(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, GLYCOSYLATION AT ASN-521.

Entry informationi

Entry nameiS29A4_MOUSE
AccessioniPrimary (citable) accession number: Q8R139
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2002
Last modified: April 1, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.