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Protein

Equilibrative nucleoside transporter 4

Gene

Slc29a4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a polyspecific organic cation transporter, efficiently transporting many organic cations such as monoamine neurotransmitters 1-methyl-4-phenylpyridinium and biogenic amines including serotonin, dopamine, norepinephrine and epinephrine. May play a role in regulating central nervous system homeostasis of monoamine neurotransmitters. May be involved in luminal transport of organic cations in the kidney and seems to use luminal proton gradient to drive organic cation reabsorptprev reabsorption. Does not seem to transport nucleoside and nucleoside analogs such as uridine, cytidine, thymidine, adenosine, inosine, guanosine, and azidothymidine. In (PubMed:16873718) adenosine is efficiently transported but in a fashion highly sensitive to extracellular pH, with maximal activity in the pH range 5.5 to 6.5. Glu-206 is essential for the cation selectivity and may function as the charge sensor for cationic substrates. Transport is chloride and sodium-independent but appears to be sensitive to changes in membrane potential. Weakly inhibited by the classical inhibitors of equilibrative nucleoside transport, dipyridamole, dilazep, and nitrobenzylthioinosine. May play a role in the regulation of extracellular adenosine concentrations in cardiac tissues, in particular during ischemia (By similarity).By similarity1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-83936. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Equilibrative nucleoside transporter 4
Alternative name(s):
Solute carrier family 29 member 4
Gene namesi
Name:Slc29a4
Synonyms:Ent4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2385330. Slc29a4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 68ExtracellularSequence analysisAdd BLAST68
Transmembranei69 – 89HelicalSequence analysisAdd BLAST21
Topological domaini90 – 101CytoplasmicSequence analysisAdd BLAST12
Transmembranei102 – 122HelicalSequence analysisAdd BLAST21
Topological domaini123 – 139ExtracellularSequence analysisAdd BLAST17
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Topological domaini161 – 166CytoplasmicSequence analysis6
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Topological domaini188 – 231ExtracellularSequence analysisAdd BLAST44
Transmembranei232 – 252HelicalSequence analysisAdd BLAST21
Topological domaini253 – 346CytoplasmicSequence analysisAdd BLAST94
Transmembranei347 – 367HelicalSequence analysisAdd BLAST21
Topological domaini368 – 376ExtracellularSequence analysis9
Transmembranei377 – 397HelicalSequence analysisAdd BLAST21
Topological domaini398 – 411CytoplasmicSequence analysisAdd BLAST14
Transmembranei412 – 432HelicalSequence analysisAdd BLAST21
Topological domaini433 – 445ExtracellularSequence analysisAdd BLAST13
Transmembranei446 – 466HelicalSequence analysisAdd BLAST21
Topological domaini467 – 481CytoplasmicSequence analysisAdd BLAST15
Transmembranei482 – 504HelicalSequence analysisAdd BLAST23
Topological domaini505 – 528ExtracellularSequence analysisAdd BLAST24

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003262521 – 528Equilibrative nucleoside transporter 4Add BLAST528

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi521N-linked (GlcNAc...)1 Publication1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8R139.
PRIDEiQ8R139.

PTM databases

iPTMnetiQ8R139.
PhosphoSitePlusiQ8R139.

Expressioni

Tissue specificityi

Expressed in heart.1 Publication

Gene expression databases

BgeeiENSMUSG00000050822.
ExpressionAtlasiQ8R139. baseline and differential.
GenevisibleiQ8R139. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000059896.

Structurei

3D structure databases

ProteinModelPortaliQ8R139.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi515 – 520Poly-Ala6

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1479. Eukaryota.
ENOG410Y3MT. LUCA.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000048076.
HOVERGENiHBG095787.
InParanoidiQ8R139.
KOiK03323.
OMAiNERNIFF.
OrthoDBiEOG091G0840.
PhylomeDBiQ8R139.
TreeFamiTF313950.

Family and domain databases

InterProiIPR030198. ENT4.
IPR002259. Eqnu_transpt.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 2 hits.
PTHR10332:SF4. PTHR10332:SF4. 2 hits.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8R139-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSIGSQRLK EPCVAATSDQ SVVTSFSFDN FQLETTAEGA QDPGIRVRGV
60 70 80 90 100
PTFTDSAVEE PVPDDRYHAI YFAMLLAGVG FLLPYNSFIT DVDYLHHKYP
110 120 130 140 150
GTSIVFDMSL TYILVALAAV LLNNVVVERL NLHTRITTGY LLALGPLLFI
160 170 180 190 200
SICDVWLQLF SHDQAYAINL AAVGTVAFGC TVQQSSFYGY TGLLPKRYTQ
210 220 230 240 250
GVMTGESTAG VMISLSRILT KLLLPDERAS TIIFFLVSAG LELLCFLLHL
260 270 280 290 300
LVRRSRFVLY YTTRPRDSRP VQAGYRVHHD VASGDIHFEH QTPALSSSRS
310 320 330 340 350
PKESPAHEVT HSNSGVYMRF DVPRPRVKRS WPTFRALLLH RYVVARVIWA
360 370 380 390 400
DMLSIAVTYF ITLCLFPGLE SEIRHCVLGE WLPILVMAVF NLSDFVGKIL
410 420 430 440 450
AALPVEWRGT HLLACSCLRV VFIPLFILCV YPSGMPALRH PAWPCVFSLL
460 470 480 490 500
MGISNGYFGS VPMILAAGKV SPKQRELAGN TMTVSYMSGL TLGSAVAYCT
510 520
YSLTRDAHGS CFQTATAAAA NDSIPVGP
Length:528
Mass (Da):58,099
Last modified:June 1, 2002 - v1
Checksum:i96379B4FBB2AD2ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC025599 mRNA. Translation: AAH25599.1.
CCDSiCCDS39364.1.
RefSeqiNP_666369.1. NM_146257.2.
XP_006504769.1. XM_006504706.2.
UniGeneiMm.125942.

Genome annotation databases

EnsembliENSMUST00000058418; ENSMUSP00000059896; ENSMUSG00000050822.
GeneIDi243328.
KEGGimmu:243328.
UCSCiuc009aiz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC025599 mRNA. Translation: AAH25599.1.
CCDSiCCDS39364.1.
RefSeqiNP_666369.1. NM_146257.2.
XP_006504769.1. XM_006504706.2.
UniGeneiMm.125942.

3D structure databases

ProteinModelPortaliQ8R139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000059896.

PTM databases

iPTMnetiQ8R139.
PhosphoSitePlusiQ8R139.

Proteomic databases

PaxDbiQ8R139.
PRIDEiQ8R139.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058418; ENSMUSP00000059896; ENSMUSG00000050822.
GeneIDi243328.
KEGGimmu:243328.
UCSCiuc009aiz.1. mouse.

Organism-specific databases

CTDi222962.
MGIiMGI:2385330. Slc29a4.

Phylogenomic databases

eggNOGiKOG1479. Eukaryota.
ENOG410Y3MT. LUCA.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000048076.
HOVERGENiHBG095787.
InParanoidiQ8R139.
KOiK03323.
OMAiNERNIFF.
OrthoDBiEOG091G0840.
PhylomeDBiQ8R139.
TreeFamiTF313950.

Enzyme and pathway databases

ReactomeiR-MMU-83936. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Miscellaneous databases

PROiQ8R139.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050822.
ExpressionAtlasiQ8R139. baseline and differential.
GenevisibleiQ8R139. MM.

Family and domain databases

InterProiIPR030198. ENT4.
IPR002259. Eqnu_transpt.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 2 hits.
PTHR10332:SF4. PTHR10332:SF4. 2 hits.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiS29A4_MOUSE
AccessioniPrimary (citable) accession number: Q8R139
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.