Reviewed, UniProtKB/Swiss-Prot Q8R139 (S29A4_MOUSE)

Names and origin

Protein names

Recommended name:
    Equilibrative nucleoside transporter 4
Alternative name(s):
    Solute carrier family 29 member 4

Gene names

Name:	Slc29a4
Synonyms:	Ent4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as a polyspecific organic cation transporter, efficiently transporting many organic cations such as monoamine neurotransmitters 1-methyl-4-phenylpyridinium and biogenic amines including serotonin, dopamine, norepinephrine and epinephrine. May play a role in regulating central nervous system homeostasis of monoamine neurotransmitters. May be involved in luminal transport of organic cations in the kidney and seems to use luminal proton gradient to drive organic cation reabsorptprev reabsorption. Does not seem to transport nucleoside and nucleoside analogs such as uridine, cytidine, thymidine, adenosine, inosine, guanosine, and azidothymidine. In (Ref.2) adenosine is efficiently transported but in a fashion highly sensitive to extracellular pH, with maximal activity in the pH range 5.5 to 6.5. Glu-206 is essential for the cation selectivity and may function as the charge sensor for cationic substrates. Transport is chloride and sodium-independent but appears to be sensitive to changes in membrane potential. Weakly inhibited by the classical inhibitors of equilibrative nucleoside transport, dipyridamole, dilazep, and nitrobenzylthioinosine. May play a role in the regulation of extracellular adenosine concentrations in cardiac tissues, in particular during ischemia By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential. Apical cell membrane; Multi-pass membrane protein By similarity. Note: Located to the plasma membranes of ventricular myocytes and vascular endothelial cells. Targeted to the apical membranes of differentiated kidney epithelial cells By similarity.
Tissue specificity	Expressed in heart. Ref.2
Post-translational modification	N-glycosylated. Ref.2
Sequence similarities	Belongs to the SLC29A transporter family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apical plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleoside transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 528	528	Equilibrative nucleoside transporter 4		PRO_0000326252
Regions
	Topological domain	1 – 68	68	Extracellular Potential
	Transmembrane	69 – 89	21	Potential
	Topological domain	90 – 101	12	Cytoplasmic Potential
	Transmembrane	102 – 122	21	Potential
	Topological domain	123 – 139	17	Extracellular Potential
	Transmembrane	140 – 160	21	Potential
	Topological domain	161 – 166	6	Cytoplasmic Potential
	Transmembrane	167 – 187	21	Potential
	Topological domain	188 – 231	44	Extracellular Potential
	Transmembrane	232 – 252	21	Potential
	Topological domain	253 – 346	94	Cytoplasmic Potential
	Transmembrane	347 – 367	21	Potential
	Topological domain	368 – 376	9	Extracellular Potential
	Transmembrane	377 – 397	21	Potential
	Topological domain	398 – 411	14	Cytoplasmic Potential
	Transmembrane	412 – 432	21	Potential
	Topological domain	433 – 445	13	Extracellular Potential
	Transmembrane	446 – 466	21	Potential
	Topological domain	467 – 481	15	Cytoplasmic Potential
	Transmembrane	482 – 504	23	Potential
	Topological domain	505 – 528	24	Extracellular Potential
	Compositional bias	515 – 520	6	Poly-Ala
Amino acid modifications
	Modified residue	220	1	Phosphothreonine Ref.3
	Glycosylation	521	1	N-linked (GlcNAc...) Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8R139-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 96379B4FBB2AD2ED	FASTA	528	58,099
	10 20 30 40 50 60 MGSIGSQRLK EPCVAATSDQ SVVTSFSFDN FQLETTAEGA QDPGIRVRGV PTFTDSAVEE 70 80 90 100 110 120 PVPDDRYHAI YFAMLLAGVG FLLPYNSFIT DVDYLHHKYP GTSIVFDMSL TYILVALAAV 130 140 150 160 170 180 LLNNVVVERL NLHTRITTGY LLALGPLLFI SICDVWLQLF SHDQAYAINL AAVGTVAFGC 190 200 210 220 230 240 TVQQSSFYGY TGLLPKRYTQ GVMTGESTAG VMISLSRILT KLLLPDERAS TIIFFLVSAG 250 260 270 280 290 300 LELLCFLLHL LVRRSRFVLY YTTRPRDSRP VQAGYRVHHD VASGDIHFEH QTPALSSSRS 310 320 330 340 350 360 PKESPAHEVT HSNSGVYMRF DVPRPRVKRS WPTFRALLLH RYVVARVIWA DMLSIAVTYF 370 380 390 400 410 420 ITLCLFPGLE SEIRHCVLGE WLPILVMAVF NLSDFVGKIL AALPVEWRGT HLLACSCLRV 430 440 450 460 470 480 VFIPLFILCV YPSGMPALRH PAWPCVFSLL MGISNGYFGS VPMILAAGKV SPKQRELAGN 490 500 510 520 TMTVSYMSGL TLGSAVAYCT YSLTRDAHGS CFQTATAAAA NDSIPVGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary tumor.
[2]	"Distribution and functional characterization of equilibrative nucleoside transporter-4, a novel cardiac adenosine transporter activated at acidic pH." Barnes K., Dobrzynski H., Foppolo S., Beal P.R., Ismat F., Scullion E.R., Sun L., Tellez J., Ritzel M.W., Claycomb W.C., Cass C.E., Young J.D., Billeter-Clark R., Boyett M.R., Baldwin S.A. Circ. Res. 99:510-519(2006) [PubMed: 16873718] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, GLYCOSYLATION AT ASN-521.
[3]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-220, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC025599 mRNA. Translation: AAH25599.1.
IPI	IPI00153278.
RefSeq	NP_666369.1.
UniGene	Mm.125942
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8R139.
Proteomic databases
PRIDE	Q8R139.
Genome annotation databases
Ensembl	ENSMUSG00000050822. Mus musculus. [Contig view]
GeneID	243328.
KEGG	mmu:243328.
Organism-specific databases
MGI	MGI:2385330. Slc29a4.
Phylogenomic databases
HOGENOM	Q8R139.
HOVERGEN	Q8R139.
OMA	Q8R139. CEVAWHM.
Gene expression databases
ArrayExpress	Q8R139.
Bgee	Q8R139.
Family and domain databases
InterPro	IPR002259. DER/eqnu_transpt. [Graphical view]
PANTHER	PTHR10332. DER/eqnu_transpt. 1 hit.
Pfam	PF01733. Nucleoside_tran. 1 hit. [Graphical view]
PRINTS	PR01130. DERENTRNSPRT.
ProDom	PD005103. DER/eqnu_transpt. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	385721.
SOURCE	Search...

Entry information

Entry name

S29A4_MOUSE

Accession

Primary (citable) accession number: Q8R139

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)