Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8R139

- S29A4_MOUSE

UniProt

Q8R139 - S29A4_MOUSE

Protein

Equilibrative nucleoside transporter 4

Gene

Slc29a4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Functions as a polyspecific organic cation transporter, efficiently transporting many organic cations such as monoamine neurotransmitters 1-methyl-4-phenylpyridinium and biogenic amines including serotonin, dopamine, norepinephrine and epinephrine. May play a role in regulating central nervous system homeostasis of monoamine neurotransmitters. May be involved in luminal transport of organic cations in the kidney and seems to use luminal proton gradient to drive organic cation reabsorptprev reabsorption. Does not seem to transport nucleoside and nucleoside analogs such as uridine, cytidine, thymidine, adenosine, inosine, guanosine, and azidothymidine. In (PubMed:16873718) adenosine is efficiently transported but in a fashion highly sensitive to extracellular pH, with maximal activity in the pH range 5.5 to 6.5. Glu-206 is essential for the cation selectivity and may function as the charge sensor for cationic substrates. Transport is chloride and sodium-independent but appears to be sensitive to changes in membrane potential. Weakly inhibited by the classical inhibitors of equilibrative nucleoside transport, dipyridamole, dilazep, and nitrobenzylthioinosine. May play a role in the regulation of extracellular adenosine concentrations in cardiac tissues, in particular during ischemia By similarity.By similarity1 Publication

    GO - Molecular functioni

    1. nucleoside transmembrane transporter activity Source: InterPro

    Keywords - Biological processi

    Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Equilibrative nucleoside transporter 4
    Alternative name(s):
    Solute carrier family 29 member 4
    Gene namesi
    Name:Slc29a4
    Synonyms:Ent4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:2385330. Slc29a4.

    Subcellular locationi

    Cell membrane Curated; Multi-pass membrane protein Curated. Apical cell membrane By similarity; Multi-pass membrane protein By similarity
    Note: Located to the plasma membranes of ventricular myocytes and vascular endothelial cells. Targeted to the apical membranes of differentiated kidney epithelial cells By similarity.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 528528Equilibrative nucleoside transporter 4PRO_0000326252Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi521 – 5211N-linked (GlcNAc...)1 Publication

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ8R139.

    PTM databases

    PhosphoSiteiQ8R139.

    Expressioni

    Tissue specificityi

    Expressed in heart.1 Publication

    Gene expression databases

    BgeeiQ8R139.
    GenevestigatoriQ8R139.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R139.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6868ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini90 – 10112CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini123 – 13917ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini161 – 1666CytoplasmicSequence Analysis
    Topological domaini188 – 23144ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini253 – 34694CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini368 – 3769ExtracellularSequence Analysis
    Topological domaini398 – 41114CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini433 – 44513ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini467 – 48115CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini505 – 52824ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei69 – 8921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei102 – 12221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei140 – 16021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei167 – 18721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei232 – 25221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei347 – 36721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei377 – 39721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei412 – 43221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei446 – 46621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei482 – 50423HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi515 – 5206Poly-Ala

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249415.
    GeneTreeiENSGT00390000002232.
    HOGENOMiHOG000048076.
    HOVERGENiHBG095787.
    InParanoidiQ8R139.
    KOiK03323.
    OMAiAHEVTHS.
    OrthoDBiEOG73Z2T0.
    PhylomeDBiQ8R139.
    TreeFamiTF313950.

    Family and domain databases

    InterProiIPR002259. Eqnu_transpt.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view]
    PANTHERiPTHR10332. PTHR10332. 1 hit.
    PfamiPF01733. Nucleoside_tran. 1 hit.
    [Graphical view]
    PRINTSiPR01130. DERENTRNSPRT.
    SUPFAMiSSF103473. SSF103473. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q8R139-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSIGSQRLK EPCVAATSDQ SVVTSFSFDN FQLETTAEGA QDPGIRVRGV    50
    PTFTDSAVEE PVPDDRYHAI YFAMLLAGVG FLLPYNSFIT DVDYLHHKYP 100
    GTSIVFDMSL TYILVALAAV LLNNVVVERL NLHTRITTGY LLALGPLLFI 150
    SICDVWLQLF SHDQAYAINL AAVGTVAFGC TVQQSSFYGY TGLLPKRYTQ 200
    GVMTGESTAG VMISLSRILT KLLLPDERAS TIIFFLVSAG LELLCFLLHL 250
    LVRRSRFVLY YTTRPRDSRP VQAGYRVHHD VASGDIHFEH QTPALSSSRS 300
    PKESPAHEVT HSNSGVYMRF DVPRPRVKRS WPTFRALLLH RYVVARVIWA 350
    DMLSIAVTYF ITLCLFPGLE SEIRHCVLGE WLPILVMAVF NLSDFVGKIL 400
    AALPVEWRGT HLLACSCLRV VFIPLFILCV YPSGMPALRH PAWPCVFSLL 450
    MGISNGYFGS VPMILAAGKV SPKQRELAGN TMTVSYMSGL TLGSAVAYCT 500
    YSLTRDAHGS CFQTATAAAA NDSIPVGP 528
    Length:528
    Mass (Da):58,099
    Last modified:June 1, 2002 - v1
    Checksum:i96379B4FBB2AD2ED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC025599 mRNA. Translation: AAH25599.1.
    CCDSiCCDS39364.1.
    RefSeqiNP_666369.1. NM_146257.2.
    XP_006504769.1. XM_006504706.1.
    UniGeneiMm.125942.

    Genome annotation databases

    EnsembliENSMUST00000058418; ENSMUSP00000059896; ENSMUSG00000050822.
    GeneIDi243328.
    KEGGimmu:243328.
    UCSCiuc009aiz.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC025599 mRNA. Translation: AAH25599.1 .
    CCDSi CCDS39364.1.
    RefSeqi NP_666369.1. NM_146257.2.
    XP_006504769.1. XM_006504706.1.
    UniGenei Mm.125942.

    3D structure databases

    ProteinModelPortali Q8R139.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8R139.

    Proteomic databases

    PRIDEi Q8R139.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000058418 ; ENSMUSP00000059896 ; ENSMUSG00000050822 .
    GeneIDi 243328.
    KEGGi mmu:243328.
    UCSCi uc009aiz.1. mouse.

    Organism-specific databases

    CTDi 222962.
    MGIi MGI:2385330. Slc29a4.

    Phylogenomic databases

    eggNOGi NOG249415.
    GeneTreei ENSGT00390000002232.
    HOGENOMi HOG000048076.
    HOVERGENi HBG095787.
    InParanoidi Q8R139.
    KOi K03323.
    OMAi AHEVTHS.
    OrthoDBi EOG73Z2T0.
    PhylomeDBi Q8R139.
    TreeFami TF313950.

    Miscellaneous databases

    NextBioi 385721.
    PROi Q8R139.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8R139.
    Genevestigatori Q8R139.

    Family and domain databases

    InterProi IPR002259. Eqnu_transpt.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view ]
    PANTHERi PTHR10332. PTHR10332. 1 hit.
    Pfami PF01733. Nucleoside_tran. 1 hit.
    [Graphical view ]
    PRINTSi PR01130. DERENTRNSPRT.
    SUPFAMi SSF103473. SSF103473. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    2. "Distribution and functional characterization of equilibrative nucleoside transporter-4, a novel cardiac adenosine transporter activated at acidic pH."
      Barnes K., Dobrzynski H., Foppolo S., Beal P.R., Ismat F., Scullion E.R., Sun L., Tellez J., Ritzel M.W., Claycomb W.C., Cass C.E., Young J.D., Billeter-Clark R., Boyett M.R., Baldwin S.A.
      Circ. Res. 99:510-519(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, GLYCOSYLATION AT ASN-521.

    Entry informationi

    Entry nameiS29A4_MOUSE
    AccessioniPrimary (citable) accession number: Q8R139
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3