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Q8R121 (ZPI_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein Z-dependent protease inhibitor

Short name=PZ-dependent protease inhibitor
Short name=PZI
Alternative name(s):
Serpin A10
Gene names
Name:Serpina10
Synonyms:Zpi
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Also inhibits factor XIa in the absence of cofactors By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Detectable in liver, but not in heart, brain, spleen, lung, kidney, skeletal muscle or testes. Ref.1

Miscellaneous

Heparin acts as an important cofactor, producing 20 to 100-fold accelerations of SERPINA10 reactions with factor Xa and factor XIa By similarity.

Sequence similarities

Belongs to the serpin family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8R121-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8R121-2)

The sequence of this isoform differs from the canonical sequence as follows:
     245-298: Missing.
Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 448427Protein Z-dependent protease inhibitor
PRO_0000032483

Regions

Region140 – 15718Heparin-binding By similarity

Sites

Site2651Essential for interaction with PROZ
Site3181Essential for interaction with PROZ
Site412 – 4132Reactive bond By similarity

Amino acid modifications

Glycosylation811N-linked (GlcNAc...) Ref.3 Ref.4
Glycosylation1841N-linked (GlcNAc...) Ref.3
Glycosylation2781N-linked (GlcNAc...) Ref.3
Glycosylation2991N-linked (GlcNAc...) Ref.3

Natural variations

Alternative sequence245 – 29854Missing in isoform 2.
VSP_014439

Experimental info

Mutagenesis961M → A: 2-fold decrease in rates of factor Xa inhibition. Ref.5
Mutagenesis991D → A: 5-fold decrease in rates of factor Xa inhibition. Ref.5
Mutagenesis2631D → A: No change in rates of factor Xa inhibition. Ref.5
Mutagenesis2641K → A: No change in rates of factor Xa inhibition. Ref.5
Mutagenesis2651Y → A: 50-fold decrease in rates of factor Xa inhibition. Ref.5
Mutagenesis3181D → A: 400-fold decrease in rates of factor Xa inhibition. Ref.5

Secondary structure

............................................................. 448
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: C7948A64BBA65B3F

FASTA44851,797
        10         20         30         40         50         60 
MRVASSLFLP VLLTEVWLVT SFNLSSHSPE ASVHLESQDY ENQTWEEYTR TDPREEEEEE 

        70         80         90        100        110        120 
EEKEEGKDEE YWLRASQQLS NETSSFGFNL LRKISMRHDG NVIFSPFGLS VAMVNLMLGT 

       130        140        150        160        170        180 
KGETKVQIEN GLNLQALSQA GPLILPALFK KVKETFSSNR DLGLSQGSFA FIHKDFDIKE 

       190        200        210        220        230        240 
TYFNLSKKYF DIEYVSINFQ NSSQARGLIN HCIVKETEGK IPKLFDEINP ETKLILVDYV 

       250        260        270        280        290        300 
LFKGKWLTPF DPSFTEADTF HLDKYRAIKV PMMYREGNFT STFDKKFRCH ILKLPYQGNA 

       310        320        330        340        350        360 
TMLVVLMEKT GDYLALEDYL TVDLVETWLQ NMKTRKMEVF FPKFKLNQRY EMHELLKQMG 

       370        380        390        400        410        420 
IRRLFSTSAD LSELSAMARN LQVSRVLQQS VLEVDERGTE AVSGTLSEII AYSMPPAIKV 

       430        440 
NRPFHFIIYE EMSRMLLFLG RVVNPTVL 

« Hide

Isoform 2 [UniParc].

Checksum: 3E0F429D3552F30F
Show »

FASTA39445,298

References

« Hide 'large scale' references
[1]"Mouse protein Z-dependent protease inhibitor cDNA."
Zhang J., Broze G.J. Jr.
Thromb. Haemost. 85:861-865(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: FVB/N.
Tissue: Liver.
[3]"Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-81; ASN-184; ASN-278 AND ASN-299.
Strain: C57BL/6.
Tissue: Plasma.
[4]"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
Bernhard O.K., Kapp E.A., Simpson R.J.
J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-81.
Strain: C57BL/6.
Tissue: Plasma.
[5]"Structural basis for catalytic activation of protein Z-dependent protease inhibitor (ZPI) by protein Z."
Huang X., Yan Y., Tu Y., Gatti J., Broze G.J. Jr., Zhou A., Olson S.T.
Blood 120:1726-1733(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 22-448, MUTAGENESIS OF MET-96; ASP-99; ASP-263; LYS-264; TYR-265 AND ASP-318.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY324633 mRNA. Translation: AAP87100.1.
BC018416 mRNA. Translation: AAH18416.1.
BC025821 mRNA. Translation: AAH25821.1.
CCDSCCDS26134.1. [Q8R121-1]
RefSeqNP_659083.2. NM_144834.3. [Q8R121-1]
UniGeneMm.29094.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4AJTX-ray2.50A22-448[»]
ProteinModelPortalQ8R121.
SMRQ8R121. Positions 68-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000048357.

Protein family/group databases

MEROPSI04.005.

PTM databases

PhosphoSiteQ8R121.

Proteomic databases

MaxQBQ8R121.
PaxDbQ8R121.
PRIDEQ8R121.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000044231; ENSMUSP00000048357; ENSMUSG00000061947. [Q8R121-1]
ENSMUST00000121625; ENSMUSP00000113644; ENSMUSG00000061947. [Q8R121-2]
GeneID217847.
KEGGmmu:217847.
UCSCuc007ovz.1. mouse. [Q8R121-1]
uc011yra.1. mouse. [Q8R121-2]

Organism-specific databases

CTD51156.
MGIMGI:2667725. Serpina10.

Phylogenomic databases

eggNOGCOG4826.
GeneTreeENSGT00750000117448.
HOGENOMHOG000238519.
HOVERGENHBG101138.
InParanoidQ8R121.
OMALLRKISM.
OrthoDBEOG7DZ8JV.
PhylomeDBQ8R121.
TreeFamTF343094.

Gene expression databases

BgeeQ8R121.
GenevestigatorQ8R121.

Family and domain databases

InterProIPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 2 hits.
ProtoNetSearch...

Other

NextBio376063.
PROQ8R121.
SOURCESearch...

Entry information

Entry nameZPI_MOUSE
AccessionPrimary (citable) accession number: Q8R121
Secondary accession number(s): Q8VCV8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot