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Q8R121

- ZPI_MOUSE

UniProt

Q8R121 - ZPI_MOUSE

Protein

Protein Z-dependent protease inhibitor

Gene

Serpina10

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Also inhibits factor XIa in the absence of cofactors By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei265 – 2651Essential for interaction with PROZ
    Sitei318 – 3181Essential for interaction with PROZ
    Sitei412 – 4132Reactive bondBy similarity

    GO - Molecular functioni

    1. heparin binding Source: UniProtKB-KW
    2. serine-type endopeptidase inhibitor activity Source: RefGenome

    GO - Biological processi

    1. blood coagulation Source: UniProtKB-KW
    2. negative regulation of endopeptidase activity Source: RefGenome
    3. regulation of proteolysis Source: RefGenome

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Keywords - Biological processi

    Blood coagulation, Hemostasis

    Keywords - Ligandi

    Heparin-binding

    Protein family/group databases

    MEROPSiI04.005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein Z-dependent protease inhibitor
    Short name:
    PZ-dependent protease inhibitor
    Short name:
    PZI
    Alternative name(s):
    Serpin A10
    Gene namesi
    Name:Serpina10
    Synonyms:Zpi
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:2667725. Serpina10.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular space Source: InterPro

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi96 – 961M → A: 2-fold decrease in rates of factor Xa inhibition. 1 Publication
    Mutagenesisi99 – 991D → A: 5-fold decrease in rates of factor Xa inhibition. 1 Publication
    Mutagenesisi263 – 2631D → A: No change in rates of factor Xa inhibition. 1 Publication
    Mutagenesisi264 – 2641K → A: No change in rates of factor Xa inhibition. 1 Publication
    Mutagenesisi265 – 2651Y → A: 50-fold decrease in rates of factor Xa inhibition. 1 Publication
    Mutagenesisi318 – 3181D → A: 400-fold decrease in rates of factor Xa inhibition. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 448427Protein Z-dependent protease inhibitorPRO_0000032483Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi81 – 811N-linked (GlcNAc...)2 Publications
    Glycosylationi184 – 1841N-linked (GlcNAc...)1 Publication
    Glycosylationi278 – 2781N-linked (GlcNAc...)1 Publication
    Glycosylationi299 – 2991N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8R121.
    PaxDbiQ8R121.
    PRIDEiQ8R121.

    PTM databases

    PhosphoSiteiQ8R121.

    Expressioni

    Tissue specificityi

    Detectable in liver, but not in heart, brain, spleen, lung, kidney, skeletal muscle or testes.1 Publication

    Gene expression databases

    BgeeiQ8R121.
    GenevestigatoriQ8R121.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000048357.

    Structurei

    Secondary structure

    1
    448
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi71 – 755
    Helixi77 – 9721
    Beta strandi102 – 1043
    Helixi106 – 11914
    Helixi123 – 13210
    Helixi135 – 1384
    Helixi144 – 15714
    Beta strandi164 – 17310
    Helixi180 – 18910
    Beta strandi194 – 1974
    Helixi202 – 21615
    Turni217 – 2193
    Beta strandi234 – 2429
    Beta strandi246 – 2483
    Helixi252 – 2543
    Beta strandi256 – 26510
    Beta strandi267 – 28418
    Turni285 – 2884
    Beta strandi289 – 2968
    Helixi297 – 2993
    Beta strandi300 – 3078
    Helixi313 – 3164
    Turni317 – 3193
    Helixi322 – 3309
    Beta strandi333 – 34210
    Beta strandi344 – 3518
    Helixi353 – 3586
    Helixi363 – 3653
    Turni372 – 3743
    Beta strandi385 – 39410
    Beta strandi417 – 4193
    Beta strandi424 – 4307
    Turni431 – 4344
    Beta strandi435 – 44410

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4AJTX-ray2.50A22-448[»]
    ProteinModelPortaliQ8R121.
    SMRiQ8R121. Positions 68-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni140 – 15718Heparin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the serpin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4826.
    GeneTreeiENSGT00750000117448.
    HOGENOMiHOG000238519.
    HOVERGENiHBG101138.
    InParanoidiQ8R121.
    OMAiLLRKISM.
    OrthoDBiEOG7DZ8JV.
    PhylomeDBiQ8R121.
    TreeFamiTF343094.

    Family and domain databases

    InterProiIPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view]
    PANTHERiPTHR11461. PTHR11461. 1 hit.
    PfamiPF00079. Serpin. 1 hit.
    [Graphical view]
    SMARTiSM00093. SERPIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF56574. SSF56574. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8R121-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRVASSLFLP VLLTEVWLVT SFNLSSHSPE ASVHLESQDY ENQTWEEYTR    50
    TDPREEEEEE EEKEEGKDEE YWLRASQQLS NETSSFGFNL LRKISMRHDG 100
    NVIFSPFGLS VAMVNLMLGT KGETKVQIEN GLNLQALSQA GPLILPALFK 150
    KVKETFSSNR DLGLSQGSFA FIHKDFDIKE TYFNLSKKYF DIEYVSINFQ 200
    NSSQARGLIN HCIVKETEGK IPKLFDEINP ETKLILVDYV LFKGKWLTPF 250
    DPSFTEADTF HLDKYRAIKV PMMYREGNFT STFDKKFRCH ILKLPYQGNA 300
    TMLVVLMEKT GDYLALEDYL TVDLVETWLQ NMKTRKMEVF FPKFKLNQRY 350
    EMHELLKQMG IRRLFSTSAD LSELSAMARN LQVSRVLQQS VLEVDERGTE 400
    AVSGTLSEII AYSMPPAIKV NRPFHFIIYE EMSRMLLFLG RVVNPTVL 448
    Length:448
    Mass (Da):51,797
    Last modified:June 1, 2002 - v1
    Checksum:iC7948A64BBA65B3F
    GO
    Isoform 2 (identifier: Q8R121-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         245-298: Missing.

    Note: May be due to a competing acceptor splice site. No experimental confirmation available.

    Show »
    Length:394
    Mass (Da):45,298
    Checksum:i3E0F429D3552F30F
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei245 – 29854Missing in isoform 2. 1 PublicationVSP_014439Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY324633 mRNA. Translation: AAP87100.1.
    BC018416 mRNA. Translation: AAH18416.1.
    BC025821 mRNA. Translation: AAH25821.1.
    CCDSiCCDS26134.1. [Q8R121-1]
    RefSeqiNP_659083.2. NM_144834.3. [Q8R121-1]
    UniGeneiMm.29094.

    Genome annotation databases

    EnsembliENSMUST00000044231; ENSMUSP00000048357; ENSMUSG00000061947. [Q8R121-1]
    ENSMUST00000121625; ENSMUSP00000113644; ENSMUSG00000061947. [Q8R121-2]
    GeneIDi217847.
    KEGGimmu:217847.
    UCSCiuc007ovz.1. mouse. [Q8R121-1]
    uc011yra.1. mouse. [Q8R121-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY324633 mRNA. Translation: AAP87100.1 .
    BC018416 mRNA. Translation: AAH18416.1 .
    BC025821 mRNA. Translation: AAH25821.1 .
    CCDSi CCDS26134.1. [Q8R121-1 ]
    RefSeqi NP_659083.2. NM_144834.3. [Q8R121-1 ]
    UniGenei Mm.29094.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4AJT X-ray 2.50 A 22-448 [» ]
    ProteinModelPortali Q8R121.
    SMRi Q8R121. Positions 68-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000048357.

    Protein family/group databases

    MEROPSi I04.005.

    PTM databases

    PhosphoSitei Q8R121.

    Proteomic databases

    MaxQBi Q8R121.
    PaxDbi Q8R121.
    PRIDEi Q8R121.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000044231 ; ENSMUSP00000048357 ; ENSMUSG00000061947 . [Q8R121-1 ]
    ENSMUST00000121625 ; ENSMUSP00000113644 ; ENSMUSG00000061947 . [Q8R121-2 ]
    GeneIDi 217847.
    KEGGi mmu:217847.
    UCSCi uc007ovz.1. mouse. [Q8R121-1 ]
    uc011yra.1. mouse. [Q8R121-2 ]

    Organism-specific databases

    CTDi 51156.
    MGIi MGI:2667725. Serpina10.

    Phylogenomic databases

    eggNOGi COG4826.
    GeneTreei ENSGT00750000117448.
    HOGENOMi HOG000238519.
    HOVERGENi HBG101138.
    InParanoidi Q8R121.
    OMAi LLRKISM.
    OrthoDBi EOG7DZ8JV.
    PhylomeDBi Q8R121.
    TreeFami TF343094.

    Miscellaneous databases

    NextBioi 376063.
    PROi Q8R121.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8R121.
    Genevestigatori Q8R121.

    Family and domain databases

    InterProi IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view ]
    PANTHERi PTHR11461. PTHR11461. 1 hit.
    Pfami PF00079. Serpin. 1 hit.
    [Graphical view ]
    SMARTi SM00093. SERPIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56574. SSF56574. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Mouse protein Z-dependent protease inhibitor cDNA."
      Zhang J., Broze G.J. Jr.
      Thromb. Haemost. 85:861-865(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: FVB/N.
      Tissue: Liver.
    3. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
      Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
      J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-81; ASN-184; ASN-278 AND ASN-299.
      Strain: C57BL/6.
      Tissue: Plasma.
    4. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
      Bernhard O.K., Kapp E.A., Simpson R.J.
      J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-81.
      Strain: C57BL/6.
      Tissue: Plasma.
    5. "Structural basis for catalytic activation of protein Z-dependent protease inhibitor (ZPI) by protein Z."
      Huang X., Yan Y., Tu Y., Gatti J., Broze G.J. Jr., Zhou A., Olson S.T.
      Blood 120:1726-1733(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 22-448, MUTAGENESIS OF MET-96; ASP-99; ASP-263; LYS-264; TYR-265 AND ASP-318.

    Entry informationi

    Entry nameiZPI_MOUSE
    AccessioniPrimary (citable) accession number: Q8R121
    Secondary accession number(s): Q8VCV8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Heparin acts as an important cofactor, producing 20 to 100-fold accelerations of SERPINA10 reactions with factor Xa and factor XIa.By similarity

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3