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Q8R121

- ZPI_MOUSE

UniProt

Q8R121 - ZPI_MOUSE

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Protein
Protein Z-dependent protease inhibitor
Gene
Serpina10, Zpi
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Also inhibits factor XIa in the absence of cofactors By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei265 – 2651Essential for interaction with PROZ
Sitei318 – 3181Essential for interaction with PROZ
Sitei412 – 4132Reactive bond By similarity

GO - Molecular functioni

  1. heparin binding Source: UniProtKB-KW
  2. serine-type endopeptidase inhibitor activity Source: RefGenome

GO - Biological processi

  1. blood coagulation Source: UniProtKB-KW
  2. negative regulation of endopeptidase activity Source: RefGenome
  3. regulation of proteolysis Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Biological processi

Blood coagulation, Hemostasis

Keywords - Ligandi

Heparin-binding

Protein family/group databases

MEROPSiI04.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Z-dependent protease inhibitor
Short name:
PZ-dependent protease inhibitor
Short name:
PZI
Alternative name(s):
Serpin A10
Gene namesi
Name:Serpina10
Synonyms:Zpi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:2667725. Serpina10.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi96 – 961M → A: 2-fold decrease in rates of factor Xa inhibition. 1 Publication
Mutagenesisi99 – 991D → A: 5-fold decrease in rates of factor Xa inhibition. 1 Publication
Mutagenesisi263 – 2631D → A: No change in rates of factor Xa inhibition. 1 Publication
Mutagenesisi264 – 2641K → A: No change in rates of factor Xa inhibition. 1 Publication
Mutagenesisi265 – 2651Y → A: 50-fold decrease in rates of factor Xa inhibition. 1 Publication
Mutagenesisi318 – 3181D → A: 400-fold decrease in rates of factor Xa inhibition. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 448427Protein Z-dependent protease inhibitor
PRO_0000032483Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi81 – 811N-linked (GlcNAc...)2 Publications
Glycosylationi184 – 1841N-linked (GlcNAc...)1 Publication
Glycosylationi278 – 2781N-linked (GlcNAc...)1 Publication
Glycosylationi299 – 2991N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8R121.
PaxDbiQ8R121.
PRIDEiQ8R121.

PTM databases

PhosphoSiteiQ8R121.

Expressioni

Tissue specificityi

Detectable in liver, but not in heart, brain, spleen, lung, kidney, skeletal muscle or testes.1 Publication

Gene expression databases

BgeeiQ8R121.
GenevestigatoriQ8R121.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048357.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi71 – 755
Helixi77 – 9721
Beta strandi102 – 1043
Helixi106 – 11914
Helixi123 – 13210
Helixi135 – 1384
Helixi144 – 15714
Beta strandi164 – 17310
Helixi180 – 18910
Beta strandi194 – 1974
Helixi202 – 21615
Turni217 – 2193
Beta strandi234 – 2429
Beta strandi246 – 2483
Helixi252 – 2543
Beta strandi256 – 26510
Beta strandi267 – 28418
Turni285 – 2884
Beta strandi289 – 2968
Helixi297 – 2993
Beta strandi300 – 3078
Helixi313 – 3164
Turni317 – 3193
Helixi322 – 3309
Beta strandi333 – 34210
Beta strandi344 – 3518
Helixi353 – 3586
Helixi363 – 3653
Turni372 – 3743
Beta strandi385 – 39410
Beta strandi417 – 4193
Beta strandi424 – 4307
Turni431 – 4344
Beta strandi435 – 44410

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AJTX-ray2.50A22-448[»]
ProteinModelPortaliQ8R121.
SMRiQ8R121. Positions 68-446.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 15718Heparin-binding By similarity
Add
BLAST

Sequence similaritiesi

Belongs to the serpin family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4826.
GeneTreeiENSGT00750000117448.
HOGENOMiHOG000238519.
HOVERGENiHBG101138.
InParanoidiQ8R121.
OMAiLLRKISM.
OrthoDBiEOG7DZ8JV.
PhylomeDBiQ8R121.
TreeFamiTF343094.

Family and domain databases

InterProiIPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8R121-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRVASSLFLP VLLTEVWLVT SFNLSSHSPE ASVHLESQDY ENQTWEEYTR    50
TDPREEEEEE EEKEEGKDEE YWLRASQQLS NETSSFGFNL LRKISMRHDG 100
NVIFSPFGLS VAMVNLMLGT KGETKVQIEN GLNLQALSQA GPLILPALFK 150
KVKETFSSNR DLGLSQGSFA FIHKDFDIKE TYFNLSKKYF DIEYVSINFQ 200
NSSQARGLIN HCIVKETEGK IPKLFDEINP ETKLILVDYV LFKGKWLTPF 250
DPSFTEADTF HLDKYRAIKV PMMYREGNFT STFDKKFRCH ILKLPYQGNA 300
TMLVVLMEKT GDYLALEDYL TVDLVETWLQ NMKTRKMEVF FPKFKLNQRY 350
EMHELLKQMG IRRLFSTSAD LSELSAMARN LQVSRVLQQS VLEVDERGTE 400
AVSGTLSEII AYSMPPAIKV NRPFHFIIYE EMSRMLLFLG RVVNPTVL 448
Length:448
Mass (Da):51,797
Last modified:June 1, 2002 - v1
Checksum:iC7948A64BBA65B3F
GO
Isoform 2 (identifier: Q8R121-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     245-298: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Show »
Length:394
Mass (Da):45,298
Checksum:i3E0F429D3552F30F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei245 – 29854Missing in isoform 2.
VSP_014439Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY324633 mRNA. Translation: AAP87100.1.
BC018416 mRNA. Translation: AAH18416.1.
BC025821 mRNA. Translation: AAH25821.1.
CCDSiCCDS26134.1. [Q8R121-1]
RefSeqiNP_659083.2. NM_144834.3. [Q8R121-1]
UniGeneiMm.29094.

Genome annotation databases

EnsembliENSMUST00000044231; ENSMUSP00000048357; ENSMUSG00000061947. [Q8R121-1]
ENSMUST00000121625; ENSMUSP00000113644; ENSMUSG00000061947. [Q8R121-2]
GeneIDi217847.
KEGGimmu:217847.
UCSCiuc007ovz.1. mouse. [Q8R121-1]
uc011yra.1. mouse. [Q8R121-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY324633 mRNA. Translation: AAP87100.1 .
BC018416 mRNA. Translation: AAH18416.1 .
BC025821 mRNA. Translation: AAH25821.1 .
CCDSi CCDS26134.1. [Q8R121-1 ]
RefSeqi NP_659083.2. NM_144834.3. [Q8R121-1 ]
UniGenei Mm.29094.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4AJT X-ray 2.50 A 22-448 [» ]
ProteinModelPortali Q8R121.
SMRi Q8R121. Positions 68-446.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000048357.

Protein family/group databases

MEROPSi I04.005.

PTM databases

PhosphoSitei Q8R121.

Proteomic databases

MaxQBi Q8R121.
PaxDbi Q8R121.
PRIDEi Q8R121.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000044231 ; ENSMUSP00000048357 ; ENSMUSG00000061947 . [Q8R121-1 ]
ENSMUST00000121625 ; ENSMUSP00000113644 ; ENSMUSG00000061947 . [Q8R121-2 ]
GeneIDi 217847.
KEGGi mmu:217847.
UCSCi uc007ovz.1. mouse. [Q8R121-1 ]
uc011yra.1. mouse. [Q8R121-2 ]

Organism-specific databases

CTDi 51156.
MGIi MGI:2667725. Serpina10.

Phylogenomic databases

eggNOGi COG4826.
GeneTreei ENSGT00750000117448.
HOGENOMi HOG000238519.
HOVERGENi HBG101138.
InParanoidi Q8R121.
OMAi LLRKISM.
OrthoDBi EOG7DZ8JV.
PhylomeDBi Q8R121.
TreeFami TF343094.

Miscellaneous databases

NextBioi 376063.
PROi Q8R121.
SOURCEi Search...

Gene expression databases

Bgeei Q8R121.
Genevestigatori Q8R121.

Family and domain databases

InterProi IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view ]
PANTHERi PTHR11461. PTHR11461. 1 hit.
Pfami PF00079. Serpin. 1 hit.
[Graphical view ]
SMARTi SM00093. SERPIN. 1 hit.
[Graphical view ]
SUPFAMi SSF56574. SSF56574. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse protein Z-dependent protease inhibitor cDNA."
    Zhang J., Broze G.J. Jr.
    Thromb. Haemost. 85:861-865(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: FVB/N.
    Tissue: Liver.
  3. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-81; ASN-184; ASN-278 AND ASN-299.
    Strain: C57BL/6.
    Tissue: Plasma.
  4. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-81.
    Strain: C57BL/6.
    Tissue: Plasma.
  5. "Structural basis for catalytic activation of protein Z-dependent protease inhibitor (ZPI) by protein Z."
    Huang X., Yan Y., Tu Y., Gatti J., Broze G.J. Jr., Zhou A., Olson S.T.
    Blood 120:1726-1733(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 22-448, MUTAGENESIS OF MET-96; ASP-99; ASP-263; LYS-264; TYR-265 AND ASP-318.

Entry informationi

Entry nameiZPI_MOUSE
AccessioniPrimary (citable) accession number: Q8R121
Secondary accession number(s): Q8VCV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Heparin acts as an important cofactor, producing 20 to 100-fold accelerations of SERPINA10 reactions with factor Xa and factor XIa By similarity.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi