Protein Z-dependent protease inhibitor precursor

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

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Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

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Q8R121 (ZPI_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 89. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Protein names

Recommended name:
Protein Z-dependent protease inhibitor
Short name=PZ-dependent protease inhibitor
Short name=PZI

Alternative name(s):
Serpin A10

Gene names

Name:	Serpina10
Synonyms:	Zpi

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Function	Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Also inhibits factor XIa in the absence of cofactors By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Detectable in liver, but not in heart, brain, spleen, lung, kidney, skeletal muscle or testes. Ref.1
Miscellaneous	Heparin acts as an important cofactor, producing 20 to 100-fold accelerations of SERPINA10 reactions with factor Xa and factor XIa By similarity.
Sequence similarities	Belongs to the serpin family.

Keywords
Biological process	Blood coagulation Hemostasis
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Heparin-binding
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW regulation of proteolysis Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Signal peptide

1 – 21

Potential

Chain

22 – 448

427

Protein Z-dependent protease inhibitor

PRO_0000032483

Region

140 – 157

Heparin-binding By similarity

Site

265

Essential for interaction with PROZ

Site

318

Essential for interaction with PROZ

Site

412 – 413

Reactive bond By similarity

Glycosylation

N-linked (GlcNAc...) Ref.3 Ref.4

Glycosylation

184

N-linked (GlcNAc...) Ref.3

Glycosylation

278

N-linked (GlcNAc...) Ref.3

Glycosylation

299

N-linked (GlcNAc...) Ref.3

Alternative sequence

245 – 298

Missing in isoform 2.

VSP_014439

Mutagenesis

M → A: 2-fold decrease in rates of factor Xa inhibition. Ref.5

Mutagenesis

D → A: 5-fold decrease in rates of factor Xa inhibition. Ref.5

Mutagenesis

263

D → A: No change in rates of factor Xa inhibition. Ref.5

Mutagenesis

264

K → A: No change in rates of factor Xa inhibition. Ref.5

Mutagenesis

265

Y → A: 50-fold decrease in rates of factor Xa inhibition. Ref.5

Mutagenesis

318

D → A: 400-fold decrease in rates of factor Xa inhibition. Ref.5

448

Helix Strand Turn

Details...

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C7948A64BBA65B3F Show »	FASTA	448	51,797
10 20 30 40 50 60 MRVASSLFLP VLLTEVWLVT SFNLSSHSPE ASVHLESQDY ENQTWEEYTR TDPREEEEEE 70 80 90 100 110 120 EEKEEGKDEE YWLRASQQLS NETSSFGFNL LRKISMRHDG NVIFSPFGLS VAMVNLMLGT 130 140 150 160 170 180 KGETKVQIEN GLNLQALSQA GPLILPALFK KVKETFSSNR DLGLSQGSFA FIHKDFDIKE 190 200 210 220 230 240 TYFNLSKKYF DIEYVSINFQ NSSQARGLIN HCIVKETEGK IPKLFDEINP ETKLILVDYV 250 260 270 280 290 300 LFKGKWLTPF DPSFTEADTF HLDKYRAIKV PMMYREGNFT STFDKKFRCH ILKLPYQGNA 310 320 330 340 350 360 TMLVVLMEKT GDYLALEDYL TVDLVETWLQ NMKTRKMEVF FPKFKLNQRY EMHELLKQMG 370 380 390 400 410 420 IRRLFSTSAD LSELSAMARN LQVSRVLQQS VLEVDERGTE AVSGTLSEII AYSMPPAIKV 430 440 NRPFHFIIYE EMSRMLLFLG RVVNPTVL « Hide
Isoform 2 [UniParc]. Checksum: 3E0F429D3552F30F Show »	FASTA	394	45,298
10 20 30 40 50 60 MRVASSLFLP VLLTEVWLVT SFNLSSHSPE ASVHLESQDY ENQTWEEYTR TDPREEEEEE 70 80 90 100 110 120 EEKEEGKDEE YWLRASQQLS NETSSFGFNL LRKISMRHDG NVIFSPFGLS VAMVNLMLGT 130 140 150 160 170 180 KGETKVQIEN GLNLQALSQA GPLILPALFK KVKETFSSNR DLGLSQGSFA FIHKDFDIKE 190 200 210 220 230 240 TYFNLSKKYF DIEYVSINFQ NSSQARGLIN HCIVKETEGK IPKLFDEINP ETKLILVDYV 250 260 270 280 290 300 LFKGNATMLV VLMEKTGDYL ALEDYLTVDL VETWLQNMKT RKMEVFFPKF KLNQRYEMHE 310 320 330 340 350 360 LLKQMGIRRL FSTSADLSEL SAMARNLQVS RVLQQSVLEV DERGTEAVSG TLSEIIAYSM 370 380 390 PPAIKVNRPF HFIIYEEMSR MLLFLGRVVN PTVL « Hide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse protein Z-dependent protease inhibitor cDNA." Zhang J., Broze G.J. Jr. Thromb. Haemost. 85:861-865(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Strain: BALB/c. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: FVB/N. Tissue: Liver.
[3]	"Proteome-wide characterization of N-glycosylation events by diagonal chromatography." Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K. J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-81; ASN-184; ASN-278 AND ASN-299, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
[4]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-81, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
[5]	"Structural basis for catalytic activation of protein Z-dependent protease inhibitor (ZPI) by protein Z." Huang X., Yan Y., Tu Y., Gatti J., Broze G.J. Jr., Zhou A., Olson S.T. Blood 120:1726-1733(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 22-448, MUTAGENESIS OF MET-96; ASP-99; ASP-263; LYS-264; TYR-265 AND ASP-318.
+	Additional computationally mapped references.

EMBL
GenBank
DDBJ

AY324633 mRNA. Translation: AAP87100.1.
BC018416 mRNA. Translation: AAH18416.1.
BC025821 mRNA. Translation: AAH25821.1.

IPI

IPI00153258.
IPI00474734.

RefSeq

NP_659083.2. NM_144834.3.

UniGene

Mm.29094.

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4AJT	X-ray	2.50	A	22-448	[»]

ProteinModelPortal

Q8R121.

SMR

Q8R121. Positions 68-446.

ModBase

Search...

STRING

10090.ENSMUSP00000048357.

MEROPS

I04.005.

PhosphoSite

Q8R121.

PaxDb

Q8R121.

PRIDE

Q8R121.

StructuralBiologyKnowledgebase

Search...

Ensembl

ENSMUST00000044231; ENSMUSP00000048357; ENSMUSG00000061947.
ENSMUST00000121625; ENSMUSP00000113644; ENSMUSG00000061947.

GeneID

217847.

KEGG

mmu:217847.

UCSC

uc007ovz.1. mouse.
uc011yra.1. mouse.

CTD

51156.

MGI

MGI:2667725. Serpina10.

eggNOG

COG4826.

GeneTree

ENSGT00560000076649.

HOGENOM

HOG000238519.

HOVERGEN

HBG101138.

InParanoid

Q8R121.

OMA

LLRKISM.

OrthoDB

EOG4VT5X7.

Bgee

Q8R121.

Genevestigator

Q8R121.

GermOnline

ENSMUSG00000061947. Mus musculus.

InterPro

IPR000295. Prot_inh_Lserp2.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]

PANTHER

PTHR11461. PTHR11461. 1 hit.

Pfam

PF00079. Serpin. 1 hit.
[Graphical view]

PRINTS

PR00780. LEUSERPINII.

SMART

SM00093. SERPIN. 1 hit.
[Graphical view]

SUPFAM

SSF56574. Prot_inh_serpin. 1 hit.

ProtoNet

Search...

NextBio

376063.

SOURCE

Search...

Entry name

ZPI_MOUSE

Accession

Primary (citable) accession number: Q8R121
Secondary accession number(s): Q8VCV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8R121-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8R121-2)

The sequence of this isoform differs from the canonical sequence as follows:
245-298: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.