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Protein

Mitoferrin-2

Gene

Slc25a28

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Mitochondrial iron transporter that mediates iron uptake. Probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe2+, is then probably delivered to ferrochelatase to catalyze Fe2+ incorporation into protoprophyrin IX to make heme (Probable).2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

ReactomeiR-MMU-1362409. Mitochondrial iron-sulfur cluster biogenesis.

Protein family/group databases

TCDBi2.A.29.5.4. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitoferrin-2
Alternative name(s):
Mitochondrial RNA-splicing protein 3/4 homolog
Short name:
MRS3/4
Mitochondrial iron transporter 2
Solute carrier family 25 member 28
Gene namesi
Name:Slc25a28
Synonyms:Mfrn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2180509. Slc25a28.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei72 – 9120Helical; Name=1Sequence analysisAdd
BLAST
Transmembranei133 – 15220Helical; Name=2Sequence analysisAdd
BLAST
Transmembranei170 – 18920Helical; Name=3Sequence analysisAdd
BLAST
Transmembranei227 – 24620Helical; Name=4Sequence analysisAdd
BLAST
Transmembranei261 – 28020Helical; Name=5Sequence analysisAdd
BLAST
Transmembranei327 – 34620Helical; Name=6Sequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364Mitoferrin-2PRO_0000235256Add
BLAST

Proteomic databases

MaxQBiQ8R0Z5.
PaxDbiQ8R0Z5.
PeptideAtlasiQ8R0Z5.
PRIDEiQ8R0Z5.

PTM databases

PhosphoSiteiQ8R0Z5.

Expressioni

Tissue specificityi

Ubiquitously expressed at low level. Expressed at higher level in heart, liver, kidney and testis. Expression does not vary during erythroid maturation.2 Publications

Gene expression databases

BgeeiENSMUSG00000040414.
CleanExiMM_SLC25A28.
GenevisibleiQ8R0Z5. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036913.

Structurei

3D structure databases

ProteinModelPortaliQ8R0Z5.
SMRiQ8R0Z5. Positions 70-363.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati70 – 15889Solcar 1Add
BLAST
Repeati168 – 25285Solcar 2Add
BLAST
Repeati263 – 35290Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0760. Eukaryota.
ENOG410XNT2. LUCA.
GeneTreeiENSGT00550000074721.
HOGENOMiHOG000038810.
HOVERGENiHBG079464.
InParanoidiQ8R0Z5.
KOiK15113.
OMAiPMRGLNI.
OrthoDBiEOG091G0PU8.
PhylomeDBiQ8R0Z5.
TreeFamiTF314118.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R0Z5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELEGRSAGG VAGGPAAGPG RSPGESALLD GWLQRGVGRG AGGGEAGAYQ
60 70 80 90 100
PPVRLDPESG PEYEALPAGA TVTTHMVAGA VAGILEHCVM YPIDCVKTRM
110 120 130 140 150
QSLQPDPAAR YRNVLEALWR IMRTEGLWRP MRGLNVTATG AGPAHALYFA
160 170 180 190 200
CYEKLKKTLS DVIHPGGNSH IANGAAGCVA TLLHDAAMNP AEVVKQRMQM
210 220 230 240 250
YNSPYHRVTD CVRAVWQNEG AGAFYRSYTT QLTMNVPFQA IHFMTYEFLQ
260 270 280 290 300
EHFNPQRRYN PSSHVLCGAC AGAVAAAATT PLDVCKTLLN TQESLALNSN
310 320 330 340 350
ITGHITGMAS AFRTVYQVGG VTAYFRGVQA RVIYQIPSTA IAWSVYEFFK
360
YLITKRQEEW RAGK
Length:364
Mass (Da):39,358
Last modified:June 1, 2002 - v1
Checksum:iE21D1DED9E9D5AC5
GO
Isoform 2 (identifier: Q8R0Z5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-187: Missing.

Show »
Length:177
Mass (Da):20,038
Checksum:iE088099CBEFC2848
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti276 – 2761A → D in AAH23172 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 187187Missing in isoform 2. 2 PublicationsVSP_018416Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF377993 mRNA. Translation: AAM46109.1.
AF377994 mRNA. Translation: AAM46110.1.
BC023172 mRNA. Translation: AAH23172.1.
BC025908 mRNA. Translation: AAH25908.1.
CCDSiCCDS29834.1. [Q8R0Z5-1]
RefSeqiNP_660138.1. NM_145156.1. [Q8R0Z5-1]
XP_006527161.1. XM_006527098.2. [Q8R0Z5-2]
UniGeneiMm.23827.

Genome annotation databases

EnsembliENSMUST00000046038; ENSMUSP00000036913; ENSMUSG00000040414. [Q8R0Z5-1]
GeneIDi246696.
KEGGimmu:246696.
UCSCiuc008hom.2. mouse. [Q8R0Z5-2]
uc008hon.2. mouse. [Q8R0Z5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF377993 mRNA. Translation: AAM46109.1.
AF377994 mRNA. Translation: AAM46110.1.
BC023172 mRNA. Translation: AAH23172.1.
BC025908 mRNA. Translation: AAH25908.1.
CCDSiCCDS29834.1. [Q8R0Z5-1]
RefSeqiNP_660138.1. NM_145156.1. [Q8R0Z5-1]
XP_006527161.1. XM_006527098.2. [Q8R0Z5-2]
UniGeneiMm.23827.

3D structure databases

ProteinModelPortaliQ8R0Z5.
SMRiQ8R0Z5. Positions 70-363.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036913.

Protein family/group databases

TCDBi2.A.29.5.4. the mitochondrial carrier (mc) family.

PTM databases

PhosphoSiteiQ8R0Z5.

Proteomic databases

MaxQBiQ8R0Z5.
PaxDbiQ8R0Z5.
PeptideAtlasiQ8R0Z5.
PRIDEiQ8R0Z5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046038; ENSMUSP00000036913; ENSMUSG00000040414. [Q8R0Z5-1]
GeneIDi246696.
KEGGimmu:246696.
UCSCiuc008hom.2. mouse. [Q8R0Z5-2]
uc008hon.2. mouse. [Q8R0Z5-1]

Organism-specific databases

CTDi81894.
MGIiMGI:2180509. Slc25a28.

Phylogenomic databases

eggNOGiKOG0760. Eukaryota.
ENOG410XNT2. LUCA.
GeneTreeiENSGT00550000074721.
HOGENOMiHOG000038810.
HOVERGENiHBG079464.
InParanoidiQ8R0Z5.
KOiK15113.
OMAiPMRGLNI.
OrthoDBiEOG091G0PU8.
PhylomeDBiQ8R0Z5.
TreeFamiTF314118.

Enzyme and pathway databases

ReactomeiR-MMU-1362409. Mitochondrial iron-sulfur cluster biogenesis.

Miscellaneous databases

PROiQ8R0Z5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040414.
CleanExiMM_SLC25A28.
GenevisibleiQ8R0Z5. MM.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMFRN2_MOUSE
AccessioniPrimary (citable) accession number: Q8R0Z5
Secondary accession number(s): Q8K4J8, Q8K4J9, Q8R578
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.