Reviewed, UniProtKB/Swiss-Prot Q8R0X7 (SGPL1_MOUSE)

Names and origin

Protein names

Recommended name:
    Sphingosine-1-phosphate lyase 1
      Short name=SP-lyase
      Short name=mSPL
    EC=4.1.2.27
Alternative name(s):
    Sphingosine-1-phosphate aldolase

Gene names

Name:

Sgpl1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis.
Catalytic activity	Sphinganine 1-phosphate = phosphoethanolamine + palmitaldehyde.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Lipid metabolism; sphingolipid metabolism.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity.
Tissue specificity	Highest levels are found in liver, followed by kidney, lung, heart and brain.
Sequence similarities	Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.

Ontologies

Keywords
Biological process	Apoptosis Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Gene Ontology (GO)
Biological process	apoptosis Inferred from sequence or structural similarity. Source: UniProtKB carboxylic acid metabolic process Inferred from electronic annotation. Source: InterPro ceramide metabolic process Inferred from sequence or structural similarity. Source: UniProtKB face morphogenesis Inferred from mutant phenotype. Source: MGI fibroblast migration Inferred from mutant phenotype. Source: MGI hemopoiesis Inferred from mutant phenotype. Source: MGI kidney development Inferred from mutant phenotype. Source: MGI nitrogen compound metabolic process Inferred from mutant phenotype. Source: MGI palate development Inferred from mutant phenotype. Source: MGI platelet-derived growth factor receptor signaling pathway Inferred from mutant phenotype. Source: MGI post-embryonic development Inferred from mutant phenotype. Source: MGI regulation of multicellular organism growth Inferred from mutant phenotype. Source: MGI skeletal system morphogenesis Inferred from mutant phenotype. Source: MGI vasculogenesis Inferred from mutant phenotype. Source: MGI
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carboxy-lyase activity Inferred from electronic annotation. Source: InterPro pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro sphinganine-1-phosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Sphingosine-1-phosphate lyase 1		PRO_0000147013
Regions
	Topological domain	1 – 40	40	Lumenal Potential
	Transmembrane	41 – 61	21	Signal-anchor for type III membrane protein Potential
	Topological domain	62 – 568	507	Cytoplasmic Potential
Amino acid modifications
	Modified residue	353	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	305	1	A → T in AAC03768. Ref.1
	Sequence conflict	473	1	N → K in BAC31437. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8R0X7-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: FA5D52E4E49DF09E	FASTA	568	63,677
	10 20 30 40 50 60 MPGTDLLKLK DFEPYLEILE SYSTKAKNYV NGYCTKYEPW QLIAWSVLCT LLIVWVYELI 70 80 90 100 110 120 FQPESLWSRF KKKLFKLIRK MPFIGRKIEQ QVSKAKKDLV KNMPFLKVDK DYVKTLPAQG 130 140 150 160 170 180 MGTAEVLERL KEYSSMDGSW QEGKASGAVY NGEPKLTELL VQAYGEFTWS NPLHPDIFPG 190 200 210 220 230 240 LRKLEAEIVR MTCSLFNGGP DSCGCVTSGG TESILMACKA YRDLALEKGI KTPEIVAPES 250 260 270 280 290 300 AHAAFDKAAH YFGMKIVRVA LKKNMEVDVQ AMKRAISRNT AMLVCSTPQF PHGVMDPVPE 310 320 330 340 350 360 VAKLAVRYKI PLHVDACLGG FLIVFMEKAG YPLEKPFDFR VKGVTSISAD THKYGYAPKG 370 380 390 400 410 420 SSVVMYSNEK YRTYQFFVGA DWQGGVYASP SIAGSRPGGI IAACWAALMH FGENGYVEAT 430 440 450 460 470 480 KQIIKTARFL KSELENIKNI FIFGDPQLSV IALGSNDFDI YRLSNMMSAK GWNFNYLQFP 490 500 510 520 530 540 RSIHFCITLV HTRKRVAIQF LKDIRESVTQ IMKNPKAKTT GMGAIYGMAQ ATIDRKLVAE 550 560 ISSVFLDCLY TTDPVTQGNQ MNGSPKPR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the first mammalian sphingosine phosphate lyase gene and its functional expression in yeast." Zhou J., Saba J.D. Biochem. Biophys. Res. Commun. 242:502-507(1998) [PubMed: 9464245] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Bone and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF036894 mRNA. Translation: AAC03768.1. AK036747 mRNA. Translation: BAC29562.1. AK037789 mRNA. Translation: BAC29872.1. AK043024 mRNA. Translation: BAC31437.1. AK049342 mRNA. Translation: BAC33695.1. BC026135 mRNA. Translation: AAH26135.1.
IPI	IPI00320480.
PIR	JC5923.
RefSeq	NP_033189.2.
UniGene	Mm.412319
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q8R0X7.
Genome annotation databases
Ensembl	ENSMUSG00000020097. Mus musculus. [Contig view]
GeneID	20397.
KEGG	mmu:20397.
Organism-specific databases
MGI	MGI:1261415. Sgpl1.
Phylogenomic databases
HOVERGEN	Q8R0X7.
OMA	Q8R0X7. IIAACWA.
Enzyme and pathway databases
BRENDA	4.1.2.27. 244.
Gene expression databases
ArrayExpress	Q8R0X7.
Bgee	Q8R0X7.
CleanEx	MM_SGPL1.
GermOnline	ENSMUSG00000020097. Mus musculus.
Family and domain databases
InterPro	IPR002129. PyrdxlP-dep_de-COase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11999. Pyridoxal_deC. 1 hit.
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
PROSITE	PS00392. DDC_GAD_HDC_YDC. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	298346.
SOURCE	Search...

Entry information

Entry name

SGPL1_MOUSE

Accession

Primary (citable) accession number: Q8R0X7
Secondary accession number(s): O54955, Q8C942

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)