Q8R0V5 (I23O2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Indoleamine 2,3-dioxygenase 2
Short name=IDO-2
EC=1.13.11.-

Alternative name(s):
Indoleamine 2,3-dioxygenase-like protein 1
Indoleamine-pyrrole 2,3-dioxygenase-like protein 1

Gene names

Name:	Ido2
Synonyms:	Indol1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. Ref.3
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine. Ref.3
Cofactor	Binds 1 heme group per subunit By similarity.
Enzyme regulation	Activity is inhibited by D-1MT (1-methyl-D-tryptophan) and MTH-trp (methylthiohydantoin-DL-tryptophan) but not L-1MT (1-methyl-L-tryptophan) By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Tissue specificity	Highest in kidney, followed by epididymis and liver (at protein level). Detected in the tails of the spermatozoa in the testis and in the kidney tubules (at protein level). Ref.3
Sequence similarities	Belongs to the indoleamine 2,3-dioxygenase family.
Biophysicochemical properties	Kinetic parameters: Do not accept D-tryptophan as substrate. KM=38.85 mM for L-tryptophan Ref.4

Ontologies

Keywords
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophan catabolic process to kynurenine Inferred from direct assay Ref.3. Source: MGI
Cellular_component	cytoplasm Inferred from direct assay PubMed 17671174. Source: MGI
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro indoleamine 2,3-dioxygenase activity Inferred from direct assay Ref.3. Source: MGI metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Indoleamine 2,3-dioxygenase 2		PRO_0000285263
Sites
	Metal binding	340	1	Iron (heme proximal ligand) By similarity
Experimental info
	Sequence conflict	249	1	V → A in AAH26393. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8R0V5 [UniParc]. Last modified October 3, 2012. Version 2. Checksum: 8916CCFE1E8B8B35	FASTA	398	44,468
	10 20 30 40 50 60 MTLEVPLSLG RYHISEEYGF LLPNPLEALP DHYKPWMEIA LRLPHLIENR QLRAHVYRMP 70 80 90 100 110 120 LLDCRFLKSY REQRLAHMAL AAITMGFVWQ EGEGQPQKVL PRSLAIPFVE VSRNLGLPPI 130 140 150 160 170 180 LVHSDLVLTN WTKRNPEGPL EISNLETIIS FPGGESLRGF ILVTVLVEKA AVPGLKALVQ 190 200 210 220 230 240 GMEAIRQHSQ DTLLEALQQL RLSIQDITRA LAQMHDYVDP DIFYSVIRIF LSGWKDNPAM 250 260 270 280 290 300 PVGLVYEGVA TEPLKYSGGS AAQSSVLHAF DEFLGIEHCK ESVGFLHRMR DYMPPSHKAF 310 320 330 340 350 360 LEDLHVAPSL RDYILASGPG DCLMAYNQCV EALGELRSYH INVVARYIIS AATRARSRGL 370 380 390 TNPSPHALED RGTGGTAMLS FLKSVREKTM EALLCPGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
[3]	"Characterization of an indoleamine 2,3-dioxygenase-like protein found in humans and mice." Ball H.J., Sanchez-Perez A., Weiser S., Austin C.J.D., Astelbauer F., Miu J., McQuillan J.A., Stocker R., Jermiin L.S., Hunt N.H. Gene 396:203-213(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
[4]	"Evolution of vertebrate indoleamine 2,3-dioxygenases." Yuasa H.J., Takubo M., Takahashi A., Hasegawa T., Noma H., Suzuki T. J. Mol. Evol. 65:705-714(2007) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC114602 Genomic DNA. No translation available. BC026393 mRNA. Translation: AAH26393.1.
IPI	IPI00153293. IPI00991045.
UniGene	Mm.219580.
3D structure databases
HSSP	HSSP built from PDB template 2D0T based on UniProtKB P14902.
ProteinModelPortal	Q8R0V5.
SMR	Q8R0V5. Positions 12-397.
ModBase	Search...
PTM databases
PhosphoSite	Q8R0V5.
Proteomic databases
PaxDb	Q8R0V5.
PRIDE	Q8R0V5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033953; ENSMUSP00000033953; ENSMUSG00000031549.
Organism-specific databases
MGI	MGI:2142489. Ido2.
Phylogenomic databases
eggNOG	NOG73554.
GeneTree	ENSGT00390000002154.
HOGENOM	HOG000190192.
InParanoid	Q8R0V5.
OrthoDB	EOG4QNMX0.
Enzyme and pathway databases
UniPathway	UPA00333; UER00453.
Gene expression databases
CleanEx	MM_INDOL1.
Genevestigator	Q8R0V5.
Family and domain databases
InterPro	IPR000898. Indolamine_dOase. [Graphical view]
Pfam	PF01231. IDO. 1 hit. [Graphical view]
PROSITE	PS00876. IDO_1. False negative. PS00877. IDO_2. False negative. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

I23O2_MOUSE

Accession

Primary (citable) accession number: Q8R0V5
Secondary accession number(s): E9QKA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program