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Protein

Adhesion G protein-coupled receptor G3

Gene

Adgrg3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Orphan receptor that regulates migration of lymphatic endothelial cells via the small GTPases RhoA and CDC42 (By similarity). Regulates B-cell development (PubMed:24113187). Seems to signal through G-alpha(q)-proteins (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • B cell differentiation Source: UniProtKB
  • cell surface receptor signaling pathway Source: InterPro
  • G-protein coupled receptor signaling pathway Source: UniProtKB
  • negative regulation of CREB transcription factor activity Source: UniProtKB
  • negative regulation of NIK/NF-kappaB signaling Source: UniProtKB
  • regulation of cell migration Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Protein family/group databases

MEROPSiP02.024.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor G3
Alternative name(s):
G-protein coupled receptor 97
Gene namesi
Name:Adgrg3
Synonyms:Gpr97
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1859670. Adgrg3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 267249ExtracellularCuratedAdd
BLAST
Transmembranei268 – 28821Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini289 – 30214CytoplasmicCuratedAdd
BLAST
Transmembranei303 – 32321Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini324 – 34219ExtracellularCuratedAdd
BLAST
Transmembranei343 – 36321Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini364 – 3729CytoplasmicCurated
Transmembranei373 – 39321Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini394 – 42633ExtracellularCuratedAdd
BLAST
Transmembranei427 – 44721Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini448 – 46720CytoplasmicCuratedAdd
BLAST
Transmembranei468 – 48821Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini489 – 4946ExtracellularCurated
Transmembranei495 – 51521Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini516 – 54227CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: MGI
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mutant mice show altered follicular-versus-marginal zone B-lymphocyte fate decision in the spleen and decreased numbers of B220+ lymphocytes in the bone marrow.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 542524Adhesion G protein-coupled receptor G3PRO_0000012890Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence analysis
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence analysis
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence analysis
Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8R0T6.
PaxDbiQ8R0T6.
PRIDEiQ8R0T6.

PTM databases

PhosphoSiteiQ8R0T6.

Expressioni

Tissue specificityi

Specifically expressed in intestinal lymphatic endothelium.1 Publication

Gene expression databases

BgeeiQ8R0T6.
CleanExiMM_GPR97.
GenevisibleiQ8R0T6. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8R0T6. 1 interaction.
MINTiMINT-4096549.
STRINGi10090.ENSMUSP00000051079.

Structurei

3D structure databases

ProteinModelPortaliQ8R0T6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini210 – 25647GPSPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 GPS domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOGENOMiHOG000112795.
HOVERGENiHBG051822.
InParanoidiQ8R0T6.
KOiK08452.
OMAiCVFWDVT.
OrthoDBiEOG7X6M07.
TreeFamiTF321769.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR000832. GPCR_2_secretin-like.
IPR003910. GPR56/AGRG3/AGRG5.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01422. GPR56ORPHANR.
SMARTiSM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R0T6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATARSLGLL FFLLLTSDEE TTEEPRNVCR RLQEGHEYDT FDLNDTAQCF
60 70 80 90 100
TKCGQSEHSP CDVGNLQRYW LNYESYLLEN SMETVDMPFV KALIQNISTD
110 120 130 140 150
VSEDLLYSLM LSQIPRQVMQ GEDEPADGVR LPKSLFGALP GNRSAVRLAI
160 170 180 190 200
TVLDIGAGNV FKGPKLLEDK GSSVLNNRMV GLSVGQMHAT GLSEPVEITF
210 220 230 240 250
SHERQPPNVI LTCVFWDMAK GDWDSHGCST VPGDGRTVCR CDHLTFFALL
260 270 280 290 300
LRPILDLATA QTLTRISQAG SAVSMIFLAF TMVLYVAFRF SLQRFKSEDA
310 320 330 340 350
PKIHMALSIS LFLLNLTFLI NVGSSSQGPP ASCWVRAAIF HYFLLCVFTW
360 370 380 390 400
MGLEAFHLYL LAIRVFNTYF GHYFLKLSLL AWGLPVLVVI GAGSSNSYGV
410 420 430 440 450
YTIRDQENRT SLELCWFQKE PALYATVHGY FLVTFLFGAV VLALVAWKIF
460 470 480 490 500
TLPSVTAGKG QGPTWKSVLT VLGLSSLVGM TWGLAVLTPL GLSTIYVFTL
510 520 530 540
LNSLQGLFIF CWFIILYFPT QSTTASSSGT ARLDQAHSVS QE
Length:542
Mass (Da):60,072
Last modified:July 27, 2011 - v3
Checksum:iE37A23B108BA1CB3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti295 – 2951F → L in AAH64726 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK155164 mRNA. Translation: BAE33087.1.
BC026426 mRNA. Translation: AAH26426.1.
BC064726 mRNA. Translation: AAH64726.1.
CCDSiCCDS22554.1.
RefSeqiNP_766624.3. NM_173036.3.
UniGeneiMm.27995.

Genome annotation databases

EnsembliENSMUST00000051259; ENSMUSP00000051079; ENSMUSG00000060470.
GeneIDi54672.
KEGGimmu:54672.
UCSCiuc009mxp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK155164 mRNA. Translation: BAE33087.1.
BC026426 mRNA. Translation: AAH26426.1.
BC064726 mRNA. Translation: AAH64726.1.
CCDSiCCDS22554.1.
RefSeqiNP_766624.3. NM_173036.3.
UniGeneiMm.27995.

3D structure databases

ProteinModelPortaliQ8R0T6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R0T6. 1 interaction.
MINTiMINT-4096549.
STRINGi10090.ENSMUSP00000051079.

Protein family/group databases

MEROPSiP02.024.
GPCRDBiSearch...

PTM databases

PhosphoSiteiQ8R0T6.

Proteomic databases

MaxQBiQ8R0T6.
PaxDbiQ8R0T6.
PRIDEiQ8R0T6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051259; ENSMUSP00000051079; ENSMUSG00000060470.
GeneIDi54672.
KEGGimmu:54672.
UCSCiuc009mxp.2. mouse.

Organism-specific databases

CTDi222487.
MGIiMGI:1859670. Adgrg3.

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOGENOMiHOG000112795.
HOVERGENiHBG051822.
InParanoidiQ8R0T6.
KOiK08452.
OMAiCVFWDVT.
OrthoDBiEOG7X6M07.
TreeFamiTF321769.

Miscellaneous databases

NextBioi311524.
PROiQ8R0T6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R0T6.
CleanExiMM_GPR97.
GenevisibleiQ8R0T6. MM.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR000832. GPCR_2_secretin-like.
IPR003910. GPR56/AGRG3/AGRG5.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01422. GPR56ORPHANR.
SMARTiSM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NOD.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo and Kidney.
  3. "Gpr97 is essential for the follicular versus marginal zone B-lymphocyte fate decision."
    Wang J.J., Zhang L.L., Zhang H.X., Shen C.L., Lu S.Y., Kuang Y., Wan Y.H., Wang W.G., Yan H.M., Dang S.Y., Fei J., Jin X.L., Wang Z.G.
    Cell Differ. 4:E853-E853(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  4. "The orphan adhesion G protein-coupled receptor GPR97 regulates migration of lymphatic endothelial cells via the small GTPases RhoA and Cdc42."
    Valtcheva N., Primorac A., Jurisic G., Hollmen M., Detmar M.
    J. Biol. Chem. 288:35736-35748(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiAGRG3_MOUSE
AccessioniPrimary (citable) accession number: Q8R0T6
Secondary accession number(s): Q3U2Q3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: February 17, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.