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Protein

IQ motif and SEC7 domain-containing protein 1

Gene

Iqsec1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor for ARF1 and ARF6. Guanine nucleotide exchange factor activity is enhanced by lipid binding. Accelerates GTP binding by ARFs of all three classes. Guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
IQ motif and SEC7 domain-containing protein 1
Gene namesi
Name:Iqsec1
Synonyms:Kiaa0763
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1196356. Iqsec1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: At steady state, may be preferentially cytosolic.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 961961IQ motif and SEC7 domain-containing protein 1PRO_0000245607Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei104 – 1041PhosphoserineCombined sources
Modified residuei106 – 1061PhosphoserineCombined sources
Modified residuei179 – 1791PhosphoserineCombined sources
Modified residuei247 – 2471PhosphoserineCombined sources
Modified residuei251 – 2511PhosphoserineCombined sources
Modified residuei510 – 5101PhosphoserineCombined sources
Modified residuei513 – 5131PhosphoserineCombined sources
Modified residuei909 – 9091PhosphotyrosineBy similarity
Modified residuei922 – 9221PhosphoserineBy similarity
Modified residuei923 – 9231PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8R0S2.
MaxQBiQ8R0S2.
PaxDbiQ8R0S2.
PRIDEiQ8R0S2.

PTM databases

iPTMnetiQ8R0S2.
PhosphoSiteiQ8R0S2.

Expressioni

Gene expression databases

BgeeiQ8R0S2.
CleanExiMM_IQSEC1.
ExpressionAtlasiQ8R0S2. baseline and differential.
GenevisibleiQ8R0S2. MM.

Interactioni

Subunit structurei

Interacts with ARF1 and ARF6.By similarity

Protein-protein interaction databases

IntActiQ8R0S2. 2 interactions.
STRINGi10090.ENSMUSP00000098710.

Structurei

3D structure databases

ProteinModelPortaliQ8R0S2.
SMRiQ8R0S2. Positions 513-878.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini133 – 16230IQPROSITE-ProRule annotationAdd
BLAST
Domaini515 – 708194SEC7PROSITE-ProRule annotationAdd
BLAST
Domaini772 – 86493PHAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili846 – 87732Sequence analysisAdd
BLAST

Domaini

The PH domain mediates interaction with lipid membranes that contain phosphatidylinositol-4,5-bisphosphate, but does not bind membranes that lack phosphatidylinositol-4,5-bisphosphate.By similarity

Sequence similaritiesi

Belongs to the BRAG family.Curated
Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 PH domain.Curated
Contains 1 SEC7 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0931. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000113099.
HOVERGENiHBG056324.
InParanoidiQ8R0S2.
KOiK12495.
OrthoDBiEOG7SR4KP.
PhylomeDBiQ8R0S2.
TreeFamiTF323811.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR030737. IQSEC1.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PANTHERiPTHR10663:SF63. PTHR10663:SF63. 1 hit.
PfamiPF16453. IQ_SEC7_PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R0S2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWCLHCNSER TQSLLELELD SGVEGEAPSS ETGTSLDSPS AYHQGPLVPG
60 70 80 90 100
SSLSPDHYEH TSVGAYGLYA GPGPQQRTRR PRLQHSTSVL RKQAEEEAIK
110 120 130 140 150
RSRSLSESYE LSSDLQDKQV EMLERKYGGR LVTRHAARTI QTAFRQYQMN
160 170 180 190 200
KNFERLRSSM SENRMSRRIV LSNMRMQFSF EGPEKVHSSY FEGKQVSVTN
210 220 230 240 250
DGSQLGALVP SECGDLSDPA LKSPAPSSDF ADAITELEDA FSRQVKSLAE
260 270 280 290 300
SIDDALNCRS LHSEEVPASD TARARDTEPK PGLHGMDHRK LDEMTASYSD
310 320 330 340 350
VTLYIDEEEL SPPLPLSQAG DRPSSTESDL RLRSGGAAQD YWALAHKEDK
360 370 380 390 400
ADTDTSCRST PSLERPEPRL RVEHLPLLTI EPPSDSSVEL SDRSDRSSLK
410 420 430 440 450
RQSAYERSLG GQQGSPKHGP HGGPPKGLPR EEPELRPRPP RPLESHLAIN
460 470 480 490 500
GSANRQSKSE SDYSDGDNDS INSTSNSNDT INCSSESSSR DSLREQTLSK
510 520 530 540 550
QTYHKETRNS WDSPAFSNDV IRKRHYRIGL NLFNKKPEKG IQYLIERGFV
560 570 580 590 600
PDTPVGVAHF LLQRKGLSRQ MIGEFLGNRQ KQFNRDVLDC VVDEMDFSAM
610 620 630 640 650
ELDEALRKFQ AHIRVQGEAQ KVERLIEAFS QRYCVCNPGV VRQFRNPDTI
660 670 680 690 700
FILAFAIILL NTDMYSPNVK PERKMKLEDF VKNLRGVDDG EDIPRETLIG
710 720 730 740 750
IYERIRKREL KTNEDHVSQV QKVEKLIVGK KPIGSLHHGL GCVLSLPHRR
760 770 780 790 800
LVCYCRLFEV PDPNKPQKLG LHQREIFLFN DLLVVTKIFQ KKKNSVTYSF
810 820 830 840 850
RQSFSLYGMQ VLLFENQYYP NGIRLTSAVP GADIKVLINF NAPNPQDRKK
860 870 880 890 900
FTDDLRESVA EVQEMEKHRI ESELEKQKGV VRPSMSQCSS LKKESGNGTL
910 920 930 940 950
SRACLDDSYA SGEGLKRSAL SSSLRDLSEA GKRGRRSSAG SLESNVEFQP
960
FQPPQPPVLC S
Length:961
Mass (Da):108,015
Last modified:July 11, 2006 - v2
Checksum:i44E9B61CE2445325
GO
Isoform 2 (identifier: Q8R0S2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MWCLHCNSERTQSLLELELDSG → MKGDGGAVWGLMWKYCISVRTLS

Note: No experimental confirmation available.
Show »
Length:962
Mass (Da):108,009
Checksum:iAB75E35ABE8F5131
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti759 – 7591E → Q in BAD90418 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2222MWCLH…ELDSG → MKGDGGAVWGLMWKYCISVR TLS in isoform 2. 1 PublicationVSP_019759Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC121954 Genomic DNA. No translation available.
AK157963 mRNA. Translation: BAE34286.1.
BC026481 mRNA. Translation: AAH26481.1.
AK220355 mRNA. Translation: BAD90418.1.
CCDSiCCDS51850.1. [Q8R0S2-1]
RefSeqiNP_001127855.1. NM_001134383.1. [Q8R0S2-1]
UniGeneiMm.196943.
Mm.473438.

Genome annotation databases

EnsembliENSMUST00000101153; ENSMUSP00000098712; ENSMUSG00000034312. [Q8R0S2-1]
GeneIDi232227.
KEGGimmu:232227.
UCSCiuc009cxq.2. mouse. [Q8R0S2-1]
uc033itn.1. mouse. [Q8R0S2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC121954 Genomic DNA. No translation available.
AK157963 mRNA. Translation: BAE34286.1.
BC026481 mRNA. Translation: AAH26481.1.
AK220355 mRNA. Translation: BAD90418.1.
CCDSiCCDS51850.1. [Q8R0S2-1]
RefSeqiNP_001127855.1. NM_001134383.1. [Q8R0S2-1]
UniGeneiMm.196943.
Mm.473438.

3D structure databases

ProteinModelPortaliQ8R0S2.
SMRiQ8R0S2. Positions 513-878.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R0S2. 2 interactions.
STRINGi10090.ENSMUSP00000098710.

PTM databases

iPTMnetiQ8R0S2.
PhosphoSiteiQ8R0S2.

Proteomic databases

EPDiQ8R0S2.
MaxQBiQ8R0S2.
PaxDbiQ8R0S2.
PRIDEiQ8R0S2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000101153; ENSMUSP00000098712; ENSMUSG00000034312. [Q8R0S2-1]
GeneIDi232227.
KEGGimmu:232227.
UCSCiuc009cxq.2. mouse. [Q8R0S2-1]
uc033itn.1. mouse. [Q8R0S2-2]

Organism-specific databases

CTDi9922.
MGIiMGI:1196356. Iqsec1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0931. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000113099.
HOVERGENiHBG056324.
InParanoidiQ8R0S2.
KOiK12495.
OrthoDBiEOG7SR4KP.
PhylomeDBiQ8R0S2.
TreeFamiTF323811.

Miscellaneous databases

PROiQ8R0S2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R0S2.
CleanExiMM_IQSEC1.
ExpressionAtlasiQ8R0S2. baseline and differential.
GenevisibleiQ8R0S2. MM.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR030737. IQSEC1.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PANTHERiPTHR10663:SF63. PTHR10663:SF63. 1 hit.
PfamiPF16453. IQ_SEC7_PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-217 (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Inner ear.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 414-961.
    Tissue: Eye.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 432-961.
    Tissue: Fetal brain.
  5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-513, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  6. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-179, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; SER-106; SER-179; SER-247; SER-251; SER-510; SER-513 AND SER-923, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiIQEC1_MOUSE
AccessioniPrimary (citable) accession number: Q8R0S2
Secondary accession number(s): Q3TZC3, Q5DU15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: June 8, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.