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Q8R0S2 (IQEC1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
IQ motif and SEC7 domain-containing protein 1
Gene names
Name:Iqsec1
Synonyms:Kiaa0763
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length961 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin By similarity.

Subunit structure

Interacts with ARF6 By similarity.

Sequence similarities

Belongs to the BRAG family.

Contains 1 IQ domain.

Contains 1 PH domain.

Contains 1 SEC7 domain.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   Molecular functionGuanine-nucleotide releasing factor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processregulation of ARF protein signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular functionARF guanyl-nucleotide exchange factor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8R0S2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8R0S2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MWCLHCNSERTQSLLELELDSG → MKGDGGAVWGLMWKYCISVRTLS
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 961961IQ motif and SEC7 domain-containing protein 1
PRO_0000245607

Regions

Domain133 – 16230IQ
Domain515 – 708194SEC7
Domain772 – 86493PH
Coiled coil846 – 87732 Potential

Amino acid modifications

Modified residue1061Phosphoserine Ref.7
Modified residue1791Phosphoserine Ref.6 Ref.9
Modified residue3601Phosphothreonine By similarity
Modified residue5101Phosphoserine Ref.7 Ref.8
Modified residue5131Phosphoserine Ref.5 Ref.6

Natural variations

Alternative sequence1 – 2222MWCLH…ELDSG → MKGDGGAVWGLMWKYCISVR TLS in isoform 2.
VSP_019759

Experimental info

Sequence conflict7591E → Q in BAD90418. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 11, 2006. Version 2.
Checksum: 44E9B61CE2445325

FASTA961108,015
        10         20         30         40         50         60 
MWCLHCNSER TQSLLELELD SGVEGEAPSS ETGTSLDSPS AYHQGPLVPG SSLSPDHYEH 

        70         80         90        100        110        120 
TSVGAYGLYA GPGPQQRTRR PRLQHSTSVL RKQAEEEAIK RSRSLSESYE LSSDLQDKQV 

       130        140        150        160        170        180 
EMLERKYGGR LVTRHAARTI QTAFRQYQMN KNFERLRSSM SENRMSRRIV LSNMRMQFSF 

       190        200        210        220        230        240 
EGPEKVHSSY FEGKQVSVTN DGSQLGALVP SECGDLSDPA LKSPAPSSDF ADAITELEDA 

       250        260        270        280        290        300 
FSRQVKSLAE SIDDALNCRS LHSEEVPASD TARARDTEPK PGLHGMDHRK LDEMTASYSD 

       310        320        330        340        350        360 
VTLYIDEEEL SPPLPLSQAG DRPSSTESDL RLRSGGAAQD YWALAHKEDK ADTDTSCRST 

       370        380        390        400        410        420 
PSLERPEPRL RVEHLPLLTI EPPSDSSVEL SDRSDRSSLK RQSAYERSLG GQQGSPKHGP 

       430        440        450        460        470        480 
HGGPPKGLPR EEPELRPRPP RPLESHLAIN GSANRQSKSE SDYSDGDNDS INSTSNSNDT 

       490        500        510        520        530        540 
INCSSESSSR DSLREQTLSK QTYHKETRNS WDSPAFSNDV IRKRHYRIGL NLFNKKPEKG 

       550        560        570        580        590        600 
IQYLIERGFV PDTPVGVAHF LLQRKGLSRQ MIGEFLGNRQ KQFNRDVLDC VVDEMDFSAM 

       610        620        630        640        650        660 
ELDEALRKFQ AHIRVQGEAQ KVERLIEAFS QRYCVCNPGV VRQFRNPDTI FILAFAIILL 

       670        680        690        700        710        720 
NTDMYSPNVK PERKMKLEDF VKNLRGVDDG EDIPRETLIG IYERIRKREL KTNEDHVSQV 

       730        740        750        760        770        780 
QKVEKLIVGK KPIGSLHHGL GCVLSLPHRR LVCYCRLFEV PDPNKPQKLG LHQREIFLFN 

       790        800        810        820        830        840 
DLLVVTKIFQ KKKNSVTYSF RQSFSLYGMQ VLLFENQYYP NGIRLTSAVP GADIKVLINF 

       850        860        870        880        890        900 
NAPNPQDRKK FTDDLRESVA EVQEMEKHRI ESELEKQKGV VRPSMSQCSS LKKESGNGTL 

       910        920        930        940        950        960 
SRACLDDSYA SGEGLKRSAL SSSLRDLSEA GKRGRRSSAG SLESNVEFQP FQPPQPPVLC 


S

« Hide

Isoform 2 [UniParc].

Checksum: AB75E35ABE8F5131
Show »

FASTA962108,009

References

[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-217 (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Inner ear.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 414-961.
Tissue: Eye.
[4]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 432-961.
Tissue: Fetal brain.
[5]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed: 16452087] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-513, MASS SPECTROMETRY.
Tissue: Brain.
[6]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-179 AND SER-513, MASS SPECTROMETRY.
Tissue: Brain cortex.
[7]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106 AND SER-510, MASS SPECTROMETRY.
Tissue: Liver.
[8]"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry."
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M.
J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-510, MASS SPECTROMETRY.
Tissue: Melanoma.
[9]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-179, MASS SPECTROMETRY.
Tissue: Macrophage.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC121954 Genomic DNA. No translation available.
AK157963 mRNA. Translation: BAE34286.1.
BC026481 mRNA. Translation: AAH26481.1.
AK220355 mRNA. Translation: BAD90418.1.
IPIIPI00762507.
IPI00974915.
RefSeqNP_001127855.1. NM_001134383.1.
UniGeneMm.196943.
Mm.473438.

3D structure databases

ProteinModelPortalQ8R0S2.
SMRQ8R0S2. Positions 518-877.
ModBaseSearch...

Protein-protein interaction databases

IntActQ8R0S2. 2 interactions.

PTM databases

PhosphoSiteQ8R0S2.

Proteomic databases

PRIDEQ8R0S2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043863; ENSMUSP00000042960; ENSMUSG00000034312.
ENSMUST00000101153; ENSMUSP00000098712; ENSMUSG00000034312.
ENSMUST00000156834; ENSMUSP00000118802; ENSMUSG00000034312.
GeneID232227.
KEGGmmu:232227.
UCSCuc009cxq.2. mouse.
uc012epa.1. mouse.

Organism-specific databases

CTD9922.
MGIMGI:1196356. Iqsec1.
RougeSearch...

Phylogenomic databases

GeneTreeENSGT00590000083058.
HOVERGENHBG056324.
OMAKKPEKGV.
OrthoDBEOG48GW2K.

Gene expression databases

ArrayExpressQ8R0S2.
BgeeQ8R0S2.
CleanExMM_IQSEC1.
GenevestigatorQ8R0S2.
GermOnlineENSMUSG00000034312. Mus musculus.

Family and domain databases

InterProIPR000048. IQ_motif_EF-hand-BS.
IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR000904. Sec7.
IPR023394. SEC7_alpha_orthog.
[Graphical view]
Gene3DG3DSA:1.10.1000.11. G3DSA:1.10.1000.11. 1 hit.
G3DSA:2.30.29.30. PH_type. 1 hit.
KOK12495.
PfamPF01369. Sec7. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMSSF48425. Sec7. 1 hit.
PROSITEPS50096. IQ. 1 hit.
PS50003. PH_DOMAIN. False negative.
PS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio381000.
SOURCESearch...

Entry information

Entry nameIQEC1_MOUSE
AccessionPrimary (citable) accession number: Q8R0S2
Secondary accession number(s): Q3TZC3, Q5DU15
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: November 16, 2011
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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