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Protein

Cyclic AMP-dependent transcription factor ATF-7

Gene

Atf7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 3125C2H2-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. sequence-specific DNA binding Source: InterPro
  3. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP-dependent transcription factor ATF-7
Short name:
cAMP-dependent transcription factor ATF-7
Alternative name(s):
Activating transcription factor 7
Transcription factor ATF-A
Gene namesi
Name:Atf7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:2443472. Atf7.

Subcellular locationi

Nucleus PROSITE-ProRule annotation. Nucleusnucleoplasm By similarity
Note: Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear peiphery (By similarity).By similarity

GO - Cellular componenti

  1. nuclear periphery Source: UniProtKB
  2. nucleoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413Cyclic AMP-dependent transcription factor ATF-7PRO_0000076593Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511Phosphothreonine; by MAPK11By similarity
Modified residuei53 – 531PhosphothreonineBy similarity
Modified residuei101 – 1011PhosphothreonineBy similarity
Cross-linki107 – 107Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1)By similarity

Post-translational modificationi

On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity (By similarity).By similarity
Sumoylation delays nuclear localization and inhibits transactivation activity through preventing binding to TAF12. RANBP2 appears to be the specific E3 ligase (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8R0S1.
PaxDbiQ8R0S1.
PRIDEiQ8R0S1.

Expressioni

Gene expression databases

BgeeiQ8R0S1.
CleanExiMM_ATF7.
ExpressionAtlasiQ8R0S1. baseline.
GenevestigatoriQ8R0S1.

Interactioni

Subunit structurei

Homodimer; binds DNA as homodimer (By similarity). Heterodimer; heterodimerizes with other members of ATF family and with JUN family members (By similarity). Interacts with JNK2; the interaction does not phosphorylate ATF7 but acts as a docking site for other ATF-associated partners such as JUN family members. Interacts (via its transactivation domain) with TAF12 the interaction potentiates the transactivation activity and is inhibited by ATF7 sumoylation. Interacts with TAF4; the interaction inhibits the TAF12-dependent transactivation. Interacts with MAPK9; the interaction does not phosphorylate ATF7 but acts as a docking site for ATF7-associated partners such as JUN (By similarity).By similarity

Protein-protein interaction databases

BioGridi230215. 2 interactions.
IntActiQ8R0S1. 1 interaction.
MINTiMINT-7557949.

Structurei

3D structure databases

ProteinModelPortaliQ8R0S1.
SMRiQ8R0S1. Positions 1-38, 334-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini332 – 39564bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 285285Transactivation domainBy similarityAdd
BLAST
Regioni334 – 35421Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni360 – 38829Leucine-zipperPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi118 – 253136Pro-richAdd
BLAST
Compositional biasi322 – 3254Poly-Arg

Sequence similaritiesi

Belongs to the bZIP family.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation
Contains 1 C2H2-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 3125C2H2-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG306955.
GeneTreeiENSGT00390000020106.
HOVERGENiHBG004300.
InParanoidiQ8R0S1.
KOiK09045.
OrthoDBiEOG741Z31.
PhylomeDBiQ8R0S1.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR004827. bZIP.
IPR016378. TF_CRE-BP1-typ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF003153. ATF2_CRE-BP1. 1 hit.
SMARTiSM00338. BRLZ. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R0S1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDDRPFVCS APGCGQRFTN EDHLAVHKHK HEMTLKFGPA RTDSVIIADQ
60 70 80 90 100
TPTPTRFLKN CEEVGLFNEL ASSFEHEFKK ASDDDEKKGA AGPLDMSLPS
110 120 130 140 150
TPDIKIKEEE PVEVDSSPPD SPASSPCSPP LKEKEVTTKP VVISTPTPTI
160 170 180 190 200
VRPGSLPLHL GYDPLHPTLP SPTSVITQAP PSNRQIGSPT GSLPLVMHLA
210 220 230 240 250
NGQTMPMLPG PPVQMPSVIS LARPVSMVPN IPGIPGPPVN NSGSISPSGH
260 270 280 290 300
PMPSEAKMRL KATLTHQVSS INGGCGMVVG TASTMVTARP EQNQILIQHP
310 320 330 340 350
DAPSPAQPQV SPAQPTPSTG GRRRRTVDED PDERRQRFLE RNRAAASRCR
360 370 380 390 400
QKRKLWVSSL EKKAEELTSQ NIQLSNEVTL LRNEVAQLKQ LLLAHKDCPV
410
TALQKKTQGY LGK
Length:413
Mass (Da):44,608
Last modified:June 1, 2002 - v1
Checksum:iAFF4D1A7BFE71AF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026483 mRNA. Translation: AAH26483.1.
CCDSiCCDS37230.1.
RefSeqiNP_666177.1. NM_146065.1.
UniGeneiMm.208714.
Mm.475149.

Genome annotation databases

EnsembliENSMUST00000108828; ENSMUSP00000104456; ENSMUSG00000099083.
ENSMUST00000169033; ENSMUSP00000130130; ENSMUSG00000099083.
GeneIDi223922.
KEGGimmu:223922.
UCSCiuc007xwo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026483 mRNA. Translation: AAH26483.1.
CCDSiCCDS37230.1.
RefSeqiNP_666177.1. NM_146065.1.
UniGeneiMm.208714.
Mm.475149.

3D structure databases

ProteinModelPortaliQ8R0S1.
SMRiQ8R0S1. Positions 1-38, 334-394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230215. 2 interactions.
IntActiQ8R0S1. 1 interaction.
MINTiMINT-7557949.

Proteomic databases

MaxQBiQ8R0S1.
PaxDbiQ8R0S1.
PRIDEiQ8R0S1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108828; ENSMUSP00000104456; ENSMUSG00000099083.
ENSMUST00000169033; ENSMUSP00000130130; ENSMUSG00000099083.
GeneIDi223922.
KEGGimmu:223922.
UCSCiuc007xwo.1. mouse.

Organism-specific databases

CTDi11016.
MGIiMGI:2443472. Atf7.

Phylogenomic databases

eggNOGiNOG306955.
GeneTreeiENSGT00390000020106.
HOVERGENiHBG004300.
InParanoidiQ8R0S1.
KOiK09045.
OrthoDBiEOG741Z31.
PhylomeDBiQ8R0S1.

Miscellaneous databases

ChiTaRSiAtf7. mouse.
NextBioi376970.
PROiQ8R0S1.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R0S1.
CleanExiMM_ATF7.
ExpressionAtlasiQ8R0S1. baseline.
GenevestigatoriQ8R0S1.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR004827. bZIP.
IPR016378. TF_CRE-BP1-typ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF003153. ATF2_CRE-BP1. 1 hit.
SMARTiSM00338. BRLZ. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.

Entry informationi

Entry nameiATF7_MOUSE
AccessioniPrimary (citable) accession number: Q8R0S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 1, 2002
Last modified: March 4, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.