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Q8R0S1 (ATF7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclic AMP-dependent transcription factor ATF-7

Short name=cAMP-dependent transcription factor ATF-7
Alternative name(s):
Activating transcription factor 7
Transcription factor ATF-A
Gene names
Name:Atf7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length413 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family By similarity.

Subunit structure

Homodimer; binds DNA as homodimer By similarity. Heterodimer; heterodimerizes with other members of ATF family and with JUN family members By similarity. Interacts with JNK2; the interaction does not phosphorylate ATF7 but acts as a docking site for other ATF-associated partners such as JUN family members. Interacts (via its transactivation domain) with TAF12 the interaction potentiates the transactivation activity and is inhibited by ATF7 sumoylation. Interacts with TAF4; the interaction inhibits the TAF12-dependent transactivation. Interacts with MAPK9; the interaction does not phosphorylate ATF7 but acts as a docking site for ATF7-associated partners such as JUN By similarity.

Subcellular location

Nucleus By similarity. Nucleusnucleoplasm By similarity. Note: Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear peiphery By similarity.

Post-translational modification

On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity By similarity.

Sumoylation delays nuclear localization and inhibits transactivation activity through preventing binding to TAF12. RANBP2 appears to be the specific E3 ligase By similarity.

Sequence similarities

Belongs to the bZIP family.

Contains 1 bZIP (basic-leucine zipper) domain.

Contains 1 C2H2-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 413413Cyclic AMP-dependent transcription factor ATF-7
PRO_0000076593

Regions

Domain332 – 39564bZIP
Zinc finger7 – 3125C2H2-type
Region1 – 285285Transactivation domain By similarity
Region334 – 35421Basic motif By similarity
Region360 – 38829Leucine-zipper By similarity
Compositional bias118 – 253136Pro-rich
Compositional bias322 – 3254Poly-Arg

Amino acid modifications

Modified residue511Phosphothreonine; by MAPK11 Ref.2
Modified residue531Phosphothreonine Ref.2
Modified residue1011Phosphothreonine By similarity
Cross-link107Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8R0S1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: AFF4D1A7BFE71AF3

FASTA41344,608
        10         20         30         40         50         60 
MGDDRPFVCS APGCGQRFTN EDHLAVHKHK HEMTLKFGPA RTDSVIIADQ TPTPTRFLKN 

        70         80         90        100        110        120 
CEEVGLFNEL ASSFEHEFKK ASDDDEKKGA AGPLDMSLPS TPDIKIKEEE PVEVDSSPPD 

       130        140        150        160        170        180 
SPASSPCSPP LKEKEVTTKP VVISTPTPTI VRPGSLPLHL GYDPLHPTLP SPTSVITQAP 

       190        200        210        220        230        240 
PSNRQIGSPT GSLPLVMHLA NGQTMPMLPG PPVQMPSVIS LARPVSMVPN IPGIPGPPVN 

       250        260        270        280        290        300 
NSGSISPSGH PMPSEAKMRL KATLTHQVSS INGGCGMVVG TASTMVTARP EQNQILIQHP 

       310        320        330        340        350        360 
DAPSPAQPQV SPAQPTPSTG GRRRRTVDED PDERRQRFLE RNRAAASRCR QKRKLWVSSL 

       370        380        390        400        410 
EKKAEELTSQ NIQLSNEVTL LRNEVAQLKQ LLLAHKDCPV TALQKKTQGY LGK 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[2]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-51 AND THR-53, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC026483 mRNA. Translation: AAH26483.1.
CCDSCCDS37230.1.
RefSeqNP_666177.1. NM_146065.1.
UniGeneMm.208714.
Mm.475149.

3D structure databases

ProteinModelPortalQ8R0S1.
SMRQ8R0S1. Positions 1-38, 334-394.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid230215. 2 interactions.
IntActQ8R0S1. 1 interaction.
MINTMINT-7557949.

Proteomic databases

PaxDbQ8R0S1.
PRIDEQ8R0S1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000108828; ENSMUSP00000104456; ENSMUSG00000099083.
ENSMUST00000169033; ENSMUSP00000130130; ENSMUSG00000099083.
GeneID223922.
KEGGmmu:223922.
UCSCuc007xwo.1. mouse.

Organism-specific databases

CTD11016.
MGIMGI:2443472. Atf7.

Phylogenomic databases

eggNOGNOG306955.
GeneTreeENSGT00390000020106.
HOVERGENHBG004300.
KOK09045.
OMATPDVKIK.
OrthoDBEOG741Z31.
PhylomeDBQ8R0S1.

Gene expression databases

ArrayExpressQ8R0S1.
BgeeQ8R0S1.
CleanExMM_ATF7.
GenevestigatorQ8R0S1.

Family and domain databases

Gene3D3.30.160.60. 1 hit.
InterProIPR004827. bZIP.
IPR016378. TF_cAMP-dep.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00170. bZIP_1. 1 hit.
[Graphical view]
PIRSFPIRSF003153. ATF2_CRE-BP1. 1 hit.
SMARTSM00338. BRLZ. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSATF7. mouse.
NextBio376970.
PROQ8R0S1.
SOURCESearch...

Entry information

Entry nameATF7_MOUSE
AccessionPrimary (citable) accession number: Q8R0S1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot