Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q8R0N6 (HOT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyacid-oxoacid transhydrogenase, mitochondrial
Short name=HOT
EC=1.1.99.24
Alternative name(s):
Alcohol dehydrogenase iron-containing protein 1
Short name=ADHFe1
Gene names
Name:Adhfe1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG By similarity.

Catalytic activity

(S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate.

4-hydroxybutanoate + 2-oxoglutarate = succinic semialdehyde + (R)-2-hydroxyglutarate.

Subcellular location

Mitochondrion Ref.3.

Tissue specificity

Expressed in white and brown adipose tissues, liver, and kidney. Expression is differentiation-dependent during in vitro brown and white adipogenesis. Ref.3

Induction

Down-regulated of 40% in white adipose tissue of ob/ob obese mice. Ref.3

Sequence similarities

Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Ontologies

Keywords
   Cellular componentMitochondrion
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processmolecular hydrogen transport

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular componentmitochondrion

Inferred from direct assay Ref.3. Source: UniProtKB

   Molecular functionhydroxyacid-oxoacid transhydrogenase activity

Inferred from sequence or structural similarity. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8R0N6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8R0N6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 465Hydroxyacid-oxoacid transhydrogenase, mitochondrialPRO_0000322997

Natural variations

Alternative sequence1 – 4646Missing in isoform 2.
VSP_031987

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 2.
Checksum: CD1463685F788C2A

FASTA46549,937
        10         20         30         40         50         60 
MAAAARARVT HLLRHLQSTA CQCPTHSHTY SQAPGPSGKT ADYAFEMAVS NIRYGAGVTK 

        70         80         90        100        110        120 
EVGMDLQNMG AKNVCLMTDK NLSQLPPVQI VMDSLSKNGI SFQVYDDVRV EPTDGSFMDA 

       130        140        150        160        170        180 
IEFAKKGAFD AYVAVGGGST MDTCKAANLY ASSPHSEFLD YVNAPIGKGK PVTVPLKPLI 

       190        200        210        220        230        240 
AVPTTSGTGS ETTGVAIFDY EHLKVKTGIA SRAIKPTLGL VDPLHTLHMP CQVVANSGFD 

       250        260        270        280        290        300 
VLCHALESYT AIPYSMRSPC PSNPIQRPAY QGSNPISDIW AVHALQIVAK YLKRAVRNPD 

       310        320        330        340        350        360 
DLEARSKMHL ASAFAGIGFG NAGVHLCHGM SYPISGLVKT YKAKEYNVDH PLVPHGLSVV 

       370        380        390        400        410        420 
LTSPAVFTFT AQMFPERHLE TAGILGANIR TARIQDAGLV LADALRKFLF DLNVDDGLAA 

       430        440        450        460 
LGYSKDDIPS LVKGTLPQER VTKLAPRAQS EEDLSALFEA SMKLY

« Hide

Isoform 2 [UniParc].

Checksum: 510F18E8678AB656
Show »

FASTA41945,015

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Hypothalamus and Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Kidney.
[3]"Differentiation-dependent expression of Adhfe1 in adipogenesis."
Kim J.Y., Tillison K.S., Zhou S., Lee J.H., Smas C.M.
Arch. Biochem. Biophys. 464:100-111(2007) [PubMed: 17559793] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AK038853 mRNA. Translation: BAC30151.1.
AK050178 mRNA. Translation: BAC34108.1.
BC026584 mRNA. Translation: AAH26584.2.
IPIIPI00227186.
IPI00889240.
RefSeqNP_780445.1. NM_175236.4.
UniGeneMm.28514.

3D structure databases

ProteinModelPortalQ8R0N6.
SMRQ8R0N6. Positions 44-462.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8R0N6.

PTM databases

PhosphoSiteQ8R0N6.

Proteomic databases

PRIDEQ8R0N6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000144177; ENSMUSP00000116627; ENSMUSG00000025911.
GeneID76187.
KEGGmmu:76187.
UCSCuc007agl.2. mouse.
uc007agm.2. mouse.

Organism-specific databases

CTD137872.
MGIMGI:1923437. Adhfe1.

Phylogenomic databases

eggNOGroNOG10256.
GeneTreeENSGT00390000003849.
HOGENOMHBG742415.
HOVERGENHBG057032.
InParanoidQ8R0N6.
OMAYSKADIP.
OrthoDBEOG4HMJ98.

Gene expression databases

ArrayExpressQ8R0N6.
BgeeQ8R0N6.
CleanExMM_ADHFE1.
GenevestigatorQ8R0N6.

Family and domain databases

InterProIPR001670. ADH_Fe.
[Graphical view]
KOK11173.
PfamPF00465. Fe-ADH. 1 hit.
[Graphical view]
PROSITEPS00913. ADH_IRON_1. False negative.
PS00060. ADH_IRON_2. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio344737.
SOURCESearch...

Entry information

Entry nameHOT_MOUSE
AccessionPrimary (citable) accession number: Q8R0N6
Secondary accession number(s): Q78SV3, Q8BYP0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: November 16, 2011
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents