ID   MOT5_MOUSE              Reviewed;         500 AA.
AC   Q8R0M8; Q8BLA5; Q8BWE3; Q8R157;
DT   21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   24-JAN-2024, entry version 143.
DE   RecName: Full=Monocarboxylate transporter 5;
DE            Short=MCT 5;
GN   Name=Slc16a4; Synonyms=Mct5;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC   STRAIN=C57BL/6J;
RC   TISSUE=Corpora quadrigemina, Diencephalon, Hippocampus, and Kidney;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   STRAIN=FVB/N; TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Proton-linked monocarboxylate transporter. Catalyzes the
CC       rapid transport across the plasma membrane of many monocarboxylates
CC       such as lactate, pyruvate, branched-chain oxo acids derived from
CC       leucine, valine and isoleucine, and the ketone bodies acetoacetate,
CC       beta-hydroxybutyrate and acetate (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q8R0M8-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8R0M8-2; Sequence=VSP_042511;
CC       Name=3;
CC         IsoId=Q8R0M8-3; Sequence=VSP_042510;
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       Monocarboxylate porter (TC 2.A.1.13) family. {ECO:0000305}.
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DR   EMBL; AK045797; BAC32495.1; -; mRNA.
DR   EMBL; AK049873; BAC33967.1; -; mRNA.
DR   EMBL; AK052779; BAC35143.1; -; mRNA.
DR   EMBL; AK085492; BAC39456.1; -; mRNA.
DR   EMBL; AK136837; BAE23142.1; -; mRNA.
DR   EMBL; AC132405; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH466607; EDL01900.1; -; Genomic_DNA.
DR   EMBL; BC025441; AAH25441.1; -; mRNA.
DR   EMBL; BC026596; AAH26596.1; -; mRNA.
DR   CCDS; CCDS17736.1; -. [Q8R0M8-1]
DR   CCDS; CCDS79997.1; -. [Q8R0M8-2]
DR   RefSeq; NP_001297634.1; NM_001310705.1. [Q8R0M8-2]
DR   RefSeq; NP_666248.1; NM_146136.1. [Q8R0M8-1]
DR   AlphaFoldDB; Q8R0M8; -.
DR   SMR; Q8R0M8; -.
DR   STRING; 10090.ENSMUSP00000029502; -.
DR   iPTMnet; Q8R0M8; -.
DR   PhosphoSitePlus; Q8R0M8; -.
DR   PaxDb; 10090-ENSMUSP00000029502; -.
DR   ProteomicsDB; 252597; -. [Q8R0M8-1]
DR   ProteomicsDB; 252598; -. [Q8R0M8-2]
DR   ProteomicsDB; 252599; -. [Q8R0M8-3]
DR   Antibodypedia; 20096; 157 antibodies from 24 providers.
DR   DNASU; 229699; -.
DR   Ensembl; ENSMUST00000029502.14; ENSMUSP00000029502.8; ENSMUSG00000027896.16. [Q8R0M8-1]
DR   Ensembl; ENSMUST00000106723.2; ENSMUSP00000102334.2; ENSMUSG00000027896.16. [Q8R0M8-2]
DR   GeneID; 229699; -.
DR   KEGG; mmu:229699; -.
DR   UCSC; uc008qwx.1; mouse. [Q8R0M8-3]
DR   UCSC; uc008qwy.2; mouse. [Q8R0M8-1]
DR   AGR; MGI:2385183; -.
DR   CTD; 9122; -.
DR   MGI; MGI:2385183; Slc16a4.
DR   VEuPathDB; HostDB:ENSMUSG00000027896; -.
DR   eggNOG; KOG2504; Eukaryota.
DR   GeneTree; ENSGT00940000158411; -.
DR   HOGENOM; CLU_001265_59_2_1; -.
DR   InParanoid; Q8R0M8; -.
DR   OMA; CKTNGCF; -.
DR   OrthoDB; 1937075at2759; -.
DR   PhylomeDB; Q8R0M8; -.
DR   TreeFam; TF313792; -.
DR   BioGRID-ORCS; 229699; 1 hit in 78 CRISPR screens.
DR   ChiTaRS; Slc16a4; mouse.
DR   PRO; PR:Q8R0M8; -.
DR   Proteomes; UP000000589; Chromosome 3.
DR   RNAct; Q8R0M8; Protein.
DR   Bgee; ENSMUSG00000027896; Expressed in right kidney and 82 other cell types or tissues.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008028; F:monocarboxylic acid transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0015718; P:monocarboxylic acid transport; IBA:GO_Central.
DR   CDD; cd17421; MFS_MCT5; 1.
DR   Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   PANTHER; PTHR11360; MONOCARBOXYLATE TRANSPORTER; 1.
DR   PANTHER; PTHR11360:SF14; MONOCARBOXYLATE TRANSPORTER 5; 1.
DR   Pfam; PF07690; MFS_1; 2.
DR   SUPFAM; SSF103473; MFS general substrate transporter; 1.
DR   PROSITE; PS50850; MFS; 1.
DR   Genevisible; Q8R0M8; MM.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell membrane; Membrane; Reference proteome; Symport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..500
FT                   /note="Monocarboxylate transporter 5"
FT                   /id="PRO_0000416125"
FT   TOPO_DOM        1..16
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        38..58
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        80..87
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        109
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        110..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        130..153
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        154..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        175
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..195
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        196..304
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        305..325
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        326..342
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        343..363
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        364..374
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        375..395
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        396
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        397..416
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        417..430
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        431..451
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        452..463
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        464..484
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        485..500
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         350..500
FT                   /note="ITETVSQLISGWIADQNWIKKYQYHKSYLILCGVTNLLAPLATTFPLLMAYT
FT                   ILFAIFAGGYLALILPVLVDLSKNSRVHKFLGYASFFAGIAVLSGPPIAGWIYDYTQTY
FT                   TGSFYFSGTCYILSSVSLFFVPLAERWKRKQSDLLRTTIK -> KKNELSLIKVY (in
FT                   isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_042510"
FT   VAR_SEQ         451..500
FT                   /note="GWIYDYTQTYTGSFYFSGTCYILSSVSLFFVPLAERWKRKQSDLLRTTIK
FT                   -> DVTHGWTTYLMHMKPLKT (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_042511"
FT   CONFLICT        366
FT                   /note="N -> D (in Ref. 1; BAC32495)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   500 AA;  55678 MW;  80B0FFF7D0C652E7 CRC64;
     MVKKEKTPSP YTKPLDGGWG WMVVLHFFLV NVFVMGMTKT FAIFFVVFQE EFEGTSEQIG
     WIGSIMSSLR FSAGPLAAII CDVLGEKATS ILGTFLVSGG YVISSWATGI PFLCVTMGLL
     PGLGSAFLYQ VAAVVITKYF KKRLGLSTAI ARSGMGLTFL LAPFTKFLID LYDWTGALIL
     FGAIILNLVP SSMLLRPIHS QSNNNSDIEN KGSILSATEP EASYKTETSK CKETQEPFIK
     DSTMKKSEQP TTTLTVLGNQ SEEFSNRPHR NRPLLMSNGK SHKKKFVSWN CKQKLLDISL
     FRNPFFYIFT WSFLLSQLAY FIPTFHLVAR AKTLGIDVMD ASYLVSVAGI TETVSQLISG
     WIADQNWIKK YQYHKSYLIL CGVTNLLAPL ATTFPLLMAY TILFAIFAGG YLALILPVLV
     DLSKNSRVHK FLGYASFFAG IAVLSGPPIA GWIYDYTQTY TGSFYFSGTC YILSSVSLFF
     VPLAERWKRK QSDLLRTTIK
//