Q8R0L1 (STAP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal-transducing adaptor protein 2
Short name=STAP-2

Gene names

Name:

Stap2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate of protein kinase PTK6 By similarity. May play a regulatory role in the acute-phase response in systemic inflammation and may modulate STAT3 activity. Ref.1
Subunit structure	Interacts with PTK6 and CSF1R. Ref.1
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Note: The translocation to the membranes occurs in response to EGF when the protein is overexpressed in fibroblastic cells. Ref.1
Tissue specificity	Widely expressed. Ref.1
Post-translational modification	Phosphorylated on tyrosine By similarity. Phosphorylated by PTK6 at Tyr-250 modulates PTK6-mediated STAT3 activation By similarity. Ref.1
Sequence similarities	Contains 1 PH domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil SH2 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: Compara plasma membrane Inferred from direct assay Ref.1. Source: MGI
Molecular_function	phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8R0L1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8R0L1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-272: FVDSDRENGESAWAVPS → VCDVGLPGLDRDTLSGG
     273-411: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Signal-transducing adaptor protein 2		PRO_0000072240
Regions
	Domain	20 – 120	101	PH
	Domain	152 – 248	97	SH2
	Coiled coil	390 – 410	21	Potential
	Compositional bias	278 – 365	88	Pro-rich
Amino acid modifications
	Modified residue	250	1	Phosphotyrosine; by PTK6 By similarity
Natural variations
	Alternative sequence	256 – 272	17	FVDSD…WAVPS → VCDVGLPGLDRDTLSGG in isoform 2.		VSP_013401
	Alternative sequence	273 – 411	139	Missing in isoform 2.		VSP_013402

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 485C00B703C470A8	FASTA	411	45,802
	10 20 30 40 50 60 MATALSPPRG PKLKGAPPSH YYESFLEKKG PCDQDYRKFW AGLQGLAICF YNSNRDLQPL 70 80 90 100 110 120 EKLDLRLFSK LRDEALLGSS RDTAYHFSLV LRDQEVKFKV ESLESCEMWK GFILTVVELR 130 140 150 160 170 180 VPSNLTLLPG HLYMMAEVLT KEEVRRAAEV PWCFLQVSRL EAQLLLERYP ECGNLLLRPG 190 200 210 220 230 240 GDGKDSVSVT TRQILNGSPV VKHYKVKRAG SKYVIDVEDP FSCPSLEAVV NYFVTHTKRA 250 260 270 280 290 300 LVPFLLDEDY EKVLGFVDSD RENGESAWAV PSFRASGPAL PANVLKPLPP VPVSVSSQED 310 320 330 340 350 360 KLPQLPPLPQ LPDTDENYVT PIEDSPAAEY MNQDVSLSSQ AVPLKPKKPA RLPAKPPKPS 370 380 390 400 410 VVPKPDLKAI TSVWTRKLGG SSSQASSLVT RLGDITAELE EKLQKRRALE H
	Isoform 2 [UniParc]. Checksum: 7F31946967BC55F9	FASTA	272	30,644
	10 20 30 40 50 60 MATALSPPRG PKLKGAPPSH YYESFLEKKG PCDQDYRKFW AGLQGLAICF YNSNRDLQPL 70 80 90 100 110 120 EKLDLRLFSK LRDEALLGSS RDTAYHFSLV LRDQEVKFKV ESLESCEMWK GFILTVVELR 130 140 150 160 170 180 VPSNLTLLPG HLYMMAEVLT KEEVRRAAEV PWCFLQVSRL EAQLLLERYP ECGNLLLRPG 190 200 210 220 230 240 GDGKDSVSVT TRQILNGSPV VKHYKVKRAG SKYVIDVEDP FSCPSLEAVV NYFVTHTKRA 250 260 270 LVPFLLDEDY EKVLGVCDVG LPGLDRDTLS GG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif." Minoguchi M., Minoguchi S., Aki D., Joo A., Yamamoto T., Yumioka T., Matsuda T., Yoshimura A. J. Biol. Chem. 278:11182-11189(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH CSF1R, FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION, SUBCELLULAR LOCATION. Tissue: Fetal liver.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK050131 mRNA. Translation: BAC34083.1. AK050172 mRNA. Translation: BAC34107.1. BC026642 mRNA. Translation: AAH26642.1.
IPI	IPI00153216. IPI00473990.
RefSeq	NP_666046.1. NM_145934.1.
UniGene	Mm.204991.
3D structure databases
ProteinModelPortal	Q8R0L1.
SMR	Q8R0L1. Positions 21-247.
ModBase	Search...
PTM databases
PhosphoSite	Q8R0L1.
Proteomic databases
PaxDb	Q8R0L1.
PRIDE	Q8R0L1.
Protocols and materials databases
DNASU	106766.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000043785; ENSMUSP00000038130; ENSMUSG00000038781.
GeneID	106766.
KEGG	mmu:106766.
UCSC	uc008dao.1. mouse.
Organism-specific databases
CTD	55620.
MGI	MGI:2147039. Stap2.
Phylogenomic databases
eggNOG	NOG43046.
GeneTree	ENSGT00530000063841.
HOGENOM	HOG000063725.
HOVERGEN	HBG108516.
InParanoid	Q8R0L1.
OMA	LYMMAEA.
OrthoDB	EOG4X3H1R.
Gene expression databases
Bgee	Q8R0L1.
CleanEx	MM_STAP2.
Genevestigator	Q8R0L1.
GermOnline	ENSMUSG00000038781. Mus musculus.
Family and domain databases
Gene3D	2.30.29.30. 1 hit. 3.30.505.10. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR000980. SH2. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00252. SH2. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. False negative. PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	358384.
SOURCE	Search...

Entry information

Entry name

STAP2_MOUSE

Accession

Primary (citable) accession number: Q8R0L1
Secondary accession number(s): Q8BWS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program