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Q8R0L1 (STAP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal-transducing adaptor protein 2

Short name=STAP-2
Gene names
Name:Stap2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length411 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Substrate of protein kinase PTK6 By similarity. May play a regulatory role in the acute-phase response in systemic inflammation and may modulate STAT3 activity. Ref.1

Subunit structure

Interacts with PTK6 and CSF1R. Ref.1

Subcellular location

Cytoplasm. Membrane; Peripheral membrane protein. Note: The translocation to the membranes occurs in response to EGF when the protein is overexpressed in fibroblastic cells. Ref.1

Tissue specificity

Widely expressed. Ref.1

Post-translational modification

Phosphorylated on tyrosine By similarity. Phosphorylated by PTK6 at Tyr-250 modulates PTK6-mediated STAT3 activation By similarity. Ref.1

Sequence similarities

Contains 1 PH domain.

Contains 1 SH2 domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
SH2 domain
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from direct assay Ref.1. Source: MGI

   Molecular_functionprotein binding

Inferred from physical interaction Ref.1. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8R0L1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8R0L1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-272: FVDSDRENGESAWAVPS → VCDVGLPGLDRDTLSGG
     273-411: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 411411Signal-transducing adaptor protein 2
PRO_0000072240

Regions

Domain20 – 120101PH
Domain152 – 24897SH2
Coiled coil390 – 41021 Potential
Compositional bias278 – 36588Pro-rich

Amino acid modifications

Modified residue2501Phosphotyrosine; by PTK6 By similarity

Natural variations

Alternative sequence256 – 27217FVDSD…WAVPS → VCDVGLPGLDRDTLSGG in isoform 2.
VSP_013401
Alternative sequence273 – 411139Missing in isoform 2.
VSP_013402

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 485C00B703C470A8

FASTA41145,802
        10         20         30         40         50         60 
MATALSPPRG PKLKGAPPSH YYESFLEKKG PCDQDYRKFW AGLQGLAICF YNSNRDLQPL 

        70         80         90        100        110        120 
EKLDLRLFSK LRDEALLGSS RDTAYHFSLV LRDQEVKFKV ESLESCEMWK GFILTVVELR 

       130        140        150        160        170        180 
VPSNLTLLPG HLYMMAEVLT KEEVRRAAEV PWCFLQVSRL EAQLLLERYP ECGNLLLRPG 

       190        200        210        220        230        240 
GDGKDSVSVT TRQILNGSPV VKHYKVKRAG SKYVIDVEDP FSCPSLEAVV NYFVTHTKRA 

       250        260        270        280        290        300 
LVPFLLDEDY EKVLGFVDSD RENGESAWAV PSFRASGPAL PANVLKPLPP VPVSVSSQED 

       310        320        330        340        350        360 
KLPQLPPLPQ LPDTDENYVT PIEDSPAAEY MNQDVSLSSQ AVPLKPKKPA RLPAKPPKPS 

       370        380        390        400        410 
VVPKPDLKAI TSVWTRKLGG SSSQASSLVT RLGDITAELE EKLQKRRALE H 

« Hide

Isoform 2 [UniParc].

Checksum: 7F31946967BC55F9
Show »

FASTA27230,644

References

« Hide 'large scale' references
[1]"STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif."
Minoguchi M., Minoguchi S., Aki D., Joo A., Yamamoto T., Yumioka T., Matsuda T., Yoshimura A.
J. Biol. Chem. 278:11182-11189(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH CSF1R, FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION, SUBCELLULAR LOCATION.
Tissue: Fetal liver.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Colon.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK050131 mRNA. Translation: BAC34083.1.
AK050172 mRNA. Translation: BAC34107.1.
BC026642 mRNA. Translation: AAH26642.1.
CCDSCCDS28889.1. [Q8R0L1-1]
RefSeqNP_666046.1. NM_145934.2. [Q8R0L1-1]
UniGeneMm.204991.

3D structure databases

ProteinModelPortalQ8R0L1.
SMRQ8R0L1. Positions 21-247.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8R0L1.

Proteomic databases

MaxQBQ8R0L1.
PaxDbQ8R0L1.
PRIDEQ8R0L1.

Protocols and materials databases

DNASU106766.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043785; ENSMUSP00000038130; ENSMUSG00000038781. [Q8R0L1-1]
GeneID106766.
KEGGmmu:106766.
UCSCuc008dao.2. mouse. [Q8R0L1-1]

Organism-specific databases

CTD55620.
MGIMGI:2147039. Stap2.

Phylogenomic databases

eggNOGNOG43046.
GeneTreeENSGT00530000063841.
HOGENOMHOG000063725.
HOVERGENHBG108516.
InParanoidQ8R0L1.
OMAPGHLYMM.
OrthoDBEOG79KPFP.
PhylomeDBQ8R0L1.
TreeFamTF332087.

Gene expression databases

BgeeQ8R0L1.
CleanExMM_STAP2.
GenevestigatorQ8R0L1.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000980. SH2.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMSSF55550. SSF55550. 1 hit.
PROSITEPS50001. SH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio358384.
PROQ8R0L1.
SOURCESearch...

Entry information

Entry nameSTAP2_MOUSE
AccessionPrimary (citable) accession number: Q8R0L1
Secondary accession number(s): Q8BWS2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot