Reviewed, UniProtKB/Swiss-Prot Q8R0K9 (E2F4_MOUSE)

Names and origin

Protein names

Recommended name:
    Transcription factor E2F4
      Short name=E2F-4

Gene names

Name:

E2f4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein By similarity.
Subunit structure	Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. The E2F4/DP-1 dimer interacts preferentially with pocket protein RBL1, which inhibits the E2F transactivation domain. Lower affinity interaction has been found with retinoblastoma protein RB1. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Interacts with HCFC1. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 By similarity.
Subcellular location	Nucleus By similarity.
Post-translational modification	Differentially phosphorylated in vivo By similarity.
Sequence similarities	Belongs to the E2F/DP family.

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	blood circulation Inferred from mutant phenotype. Source: MGI cell volume homeostasis Inferred from mutant phenotype. Source: MGI cilium assembly Inferred from mutant phenotype. Source: MGI epithelial cell development Inferred from mutant phenotype. Source: MGI regulation of cell cycle Traceable author statement. Source: MGI regulation of cell proliferation Inferred from direct assay. Source: MGI regulation of cell size Inferred from mutant phenotype. Source: MGI regulation of transcription of G1/S-phase of mitotic cell cycle Inferred from direct assay. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Traceable author statement. Source: MGI transcription factor complex Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: MGI transcription factor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Transcription factor E2F4		PRO_0000322638
Regions
	Domain	43 – 65	23	Leucine-zipper
	DNA binding	16 – 85	70	Potential
	Region	86 – 181	96	Dimerization Potential
	Region	334 – 410	77	Transactivation Potential
	Region	387 – 404	18	Interaction with RBL1 and RBL2 Potential
	Motif	48 – 85	38	DEF box By similarity
	Motif	386 – 389	4	HCFC1-binding-motif (HBM) By similarity
	Compositional bias	9 – 12	4	Poly-Pro
	Compositional bias	308 – 324	17	Poly-Ser
Experimental info
	Sequence conflict	326	1	P → T in AAH27048. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8R0K9-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A8BD1A24B38C2B01	FASTA	410	43,833
	10 20 30 40 50 60 MAEAGPQAPP PPGTPSRHEK SLGLLTTKFV SLLQEAKDGV LDLKLAADTL AVRQKRRIYD 70 80 90 100 110 120 ITNVLEGIGL IEKKSKNSIQ WKGVGPGCNT REIADKLIEL KAEIEELQQR EQELDQHKVW 130 140 150 160 170 180 VQQSIRNVTE DVQNSCLAYV THEDICRCFA GDTLLAIRAP SGTSLEVPIP EGLNGQKKYQ 190 200 210 220 230 240 IHLKSMSGPI EVLLVNKEAW SSPPVAVPVP PPDDLLQSPP AVSTPPPLPK PALAQPQESS 250 260 270 280 290 300 PPSSPQLTTP TPVLGSTQVS EVACQTSEIA VSGSPGTENK DSGEVSSLPL GLTALDTRPL 310 320 330 340 350 360 QSSALLDSSS SSSSSSSSSS SSSSGPNPST SFEPIKADPT GVLDLPKELS EIFDPTRECM 370 380 390 400 410 SSELLEELMS SEVFAPLLRL SPPPGDHDYI YNLDESEGVC DLFDVPVLKL

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Liver and Spleen.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II and FVB/N. Tissue: Colon and Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK145950 mRNA. Translation: BAE26778.1. AK157028 mRNA. Translation: BAE33937.1. BC023859 mRNA. Translation: AAH23859.1. BC026649 mRNA. Translation: AAH26649.1. BC027048 mRNA. Translation: AAH27048.1.
IPI	IPI00280093.
RefSeq	NP_683754.1.
UniGene	Mm.34554
3D structure databases
HSSP	HSSP built from PDB template 1CF7 based on UniProtKB Q16254.
SMR	Q8R0K9. Positions 16-82.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000014859. Mus musculus. [Contig view]
GeneID	104394.
KEGG	mmu:104394.
Organism-specific databases
MGI	MGI:103012. E2f4.
Phylogenomic databases
HOGENOM	Q8R0K9.
HOVERGEN	Q8R0K9.
OMA	Q8R0K9. QDASRPS.
Gene expression databases
ArrayExpress	Q8R0K9.
Bgee	Q8R0K9.
CleanEx	MM_E2F4.
Family and domain databases
InterPro	IPR015633. E2F. IPR003316. E2F_TDP. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR12081. E2F. 1 hit.
Pfam	PF02319. E2F_TDP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	357065.
SOURCE	Search...

Entry information

Entry name

E2F4_MOUSE

Accession

Primary (citable) accession number: Q8R0K9
Secondary accession number(s): Q8R2X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)