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Protein

Transcription factor E2F4

Gene

E2f4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi16 – 85Sequence analysisAdd BLAST70

GO - Molecular functioni

GO - Biological processi

  • animal organ morphogenesis Source: MGI
  • blood circulation Source: MGI
  • cell volume homeostasis Source: MGI
  • centriole assembly Source: UniProtKB
  • cilium assembly Source: MGI
  • epithelial cell development Source: MGI
  • motile cilium assembly Source: UniProtKB
  • multi-ciliated epithelial cell differentiation Source: UniProtKB
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of cell cycle Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of cell size Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • regulation of transcription involved in G1/S transition of mitotic cell cycle Source: MGI

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCell cycle, Cilium biogenesis/degradation, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-1538133 G0 and Early G1
R-MMU-2173796 SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription
R-MMU-69231 Cyclin D associated events in G1

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F4
Short name:
E2F-4
Gene namesi
Name:E2f4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:103012 E2f4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Postnatal lethality, probably due to the absence of ciliated cells from the entire airway epithelium and the epithelium of the submucosal glands in the paranasal sinuses. In the nasal epithelium, ciliated cells are replaced by columnar secretory cells that produce mucin-like substances. In the proximal lung, reduction in Clara cell is also obrserved. The combination of no ciliated cells and excess mucous cells leads for the chronic rhinitis and increased susceptibility to opportunistic infections that cause lethality.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003226382 – 410Transcription factor E2F4Add BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei381PhosphoserineCombined sources1

Post-translational modificationi

Differentially phosphorylated in vivo.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8R0K9
MaxQBiQ8R0K9
PaxDbiQ8R0K9
PeptideAtlasiQ8R0K9
PRIDEiQ8R0K9

PTM databases

iPTMnetiQ8R0K9
PhosphoSitePlusiQ8R0K9

Expressioni

Gene expression databases

BgeeiENSMUSG00000014859
CleanExiMM_E2F4
GenevisibleiQ8R0K9 MM

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. The E2F4/TFDP1 dimer interacts preferentially with pocket protein RBL1, which inhibits the E2F transactivation domain. Lower affinity interaction has been found with retinoblastoma protein RB1. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Interacts with HCFC1. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with PML. Interacts with CEBPA (when phosphorylated) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi222608, 4 interactors
CORUMiQ8R0K9
IntActiQ8R0K9, 10 interactors
MINTiQ8R0K9
STRINGi10090.ENSMUSP00000015003

Structurei

3D structure databases

ProteinModelPortaliQ8R0K9
SMRiQ8R0K9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 65Leucine-zipperAdd BLAST23
Regioni86 – 181DimerizationSequence analysisAdd BLAST96
Regioni334 – 410TransactivationSequence analysisAdd BLAST77
Regioni387 – 404Interaction with RBL1 and RBL2Sequence analysisAdd BLAST18

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi48 – 85DEF boxBy similarityAdd BLAST38
Motifi386 – 389HCFC1-binding-motif (HBM)By similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 12Poly-Pro4
Compositional biasi308 – 324Poly-SerAdd BLAST17

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2577 Eukaryota
ENOG410XNYI LUCA
GeneTreeiENSGT00550000074403
HOGENOMiHOG000232045
HOVERGENiHBG002227
InParanoidiQ8R0K9
KOiK04682
OMAiVQNSPHT
OrthoDBiEOG091G0CJQ
PhylomeDBiQ8R0K9
TreeFamiTF105566

Family and domain databases

CDDicd14660 E2F_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR015633 E2F
IPR037241 E2F-DP_heterodim
IPR028312 E2F4
IPR032198 E2F_CC-MB
IPR003316 E2F_WHTH_DNA-bd_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12081 PTHR12081, 1 hit
PTHR12081:SF42 PTHR12081:SF42, 1 hit
PfamiView protein in Pfam
PF16421 E2F_CC-MB, 1 hit
PF02319 E2F_TDP, 1 hit
SMARTiView protein in SMART
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R0K9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAGPQAPP PPGTPSRHEK SLGLLTTKFV SLLQEAKDGV LDLKLAADTL
60 70 80 90 100
AVRQKRRIYD ITNVLEGIGL IEKKSKNSIQ WKGVGPGCNT REIADKLIEL
110 120 130 140 150
KAEIEELQQR EQELDQHKVW VQQSIRNVTE DVQNSCLAYV THEDICRCFA
160 170 180 190 200
GDTLLAIRAP SGTSLEVPIP EGLNGQKKYQ IHLKSMSGPI EVLLVNKEAW
210 220 230 240 250
SSPPVAVPVP PPDDLLQSPP AVSTPPPLPK PALAQPQESS PPSSPQLTTP
260 270 280 290 300
TPVLGSTQVS EVACQTSEIA VSGSPGTENK DSGEVSSLPL GLTALDTRPL
310 320 330 340 350
QSSALLDSSS SSSSSSSSSS SSSSGPNPST SFEPIKADPT GVLDLPKELS
360 370 380 390 400
EIFDPTRECM SSELLEELMS SEVFAPLLRL SPPPGDHDYI YNLDESEGVC
410
DLFDVPVLKL
Length:410
Mass (Da):43,833
Last modified:June 1, 2002 - v1
Checksum:iA8BD1A24B38C2B01
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti326P → T in AAH27048 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK145950 mRNA Translation: BAE26778.1
AK157028 mRNA Translation: BAE33937.1
BC023859 mRNA Translation: AAH23859.1
BC026649 mRNA Translation: AAH26649.1
BC027048 mRNA Translation: AAH27048.1
CCDSiCCDS40456.1
RefSeqiNP_683754.1, NM_148952.1
UniGeneiMm.34554

Genome annotation databases

EnsembliENSMUST00000015003; ENSMUSP00000015003; ENSMUSG00000014859
GeneIDi104394
KEGGimmu:104394
UCSCiuc009ncl.1 mouse

Similar proteinsi

Entry informationi

Entry nameiE2F4_MOUSE
AccessioniPrimary (citable) accession number: Q8R0K9
Secondary accession number(s): Q8R2X6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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