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Q8R0H9 (GGA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ADP-ribosylation factor-binding protein GGA1
Alternative name(s):
Gamma-adaptin-related protein 1
Golgi-localized, gamma ear-containing, ARF-binding protein 1
Gene names
Name:Gga1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length635 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif By similarity.

Subunit structure

Monomer. Interacts with NECAP1 By similarity. Interacts with GGA2 and GGA3. Binds to clathrin and activated ARFs, including ARF1. Interacts with RABEP1 and RABGEF1. Interacts with the type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. Binds CCDC91, P200, SYNRG, EPN4, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and UBC By similarity. Interacts with RNF11 By similarity. Interacts with CNST. Interacts with ARF6. Ref.3 Ref.4

Subcellular location

Golgi apparatustrans-Golgi network membrane; Peripheral membrane protein By similarity. Endosome membrane; Peripheral membrane protein By similarity.

Domain

The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif) By similarity.

The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis By similarity.

The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs By similarity.

The GAE domain binds accessory proteins regulating GGAs function.

Post-translational modification

Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.

Ubiquitinated By similarity.

Sequence similarities

Belongs to the GGA protein family.

Contains 1 GAE domain.

Contains 1 GAT domain.

Contains 1 VHS domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CnstQ8CBC4-32EBI-2616212,EBI-2615407

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 635635ADP-ribosylation factor-binding protein GGA1
PRO_0000212681

Regions

Domain17 – 147131VHS
Domain171 – 298128GAT
Domain506 – 627122GAE
Region114 – 273160Interaction with ARF3 By similarity
Region299 – 505207Unstructured hinge
Motif357 – 3615Autoinhibitory By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8R0H9 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 9F5F1C37F41ECCC6

FASTA63569,972
        10         20         30         40         50         60 
MEPAMEPETL EARINRATNP LNKELNWASI NSFCEQLNED FEGPPLATRL LAHKIQSPQE 

        70         80         90        100        110        120 
WEAIQALTVL ETCMKSCGKR FHDEVGKFRF LNELIKVVSP KYLGSRTSEK VKSKILELLY 

       130        140        150        160        170        180 
SWTVCLPEEV KIAEAYQMLK KQGIVKSDPK LPEDAIFPLP PPRPKNVIFE DEEKSKMLAR 

       190        200        210        220        230        240 
LLKSSHPEDL RAANKLIKEM VQEDQKRMEK ISKRVNAIEE VNNNVKLLTE MVMSHSQGAA 

       250        260        270        280        290        300 
SSSSEDLMKE LYQRCERMRP TLFRLASDTE DNDEALAEIL QANDNLTQVI NLYKQLVRGE 

       310        320        330        340        350        360 
EVNGDATASS IPGSTSALLD LSGLDLPPPG TTQPATPTRP GNQSSPEQLS ASVSLLDDEL 

       370        380        390        400        410        420 
MSLGLSDPTP PSGTSSDSVG WDNFQSSDGT ESSVPPPAQA PSMDCRPPAQ APPPTSSGLD 

       430        440        450        460        470        480 
DLDLLGKTLM QQALPPEAQQ VRWEKQQPAP RLTLRDLQSK SSSPSPGAAS LLHTTSPEPP 

       490        500        510        520        530        540 
GPPPQATPTE FSLTSITVPL ESIKPSSILP VTVYDQHGFR VLFHFARDPL PGRSDVLVVV 

       550        560        570        580        590        600 
VSMLSTAPQP IRNIVFQSAV PKVMKVRLQP PSGTELPAFN PIVHPSAITQ VLLLANPQKE 

       610        620        630 
KVRLRYKLIF TMGDQTYNEM GDVDQFPPPE TWGSL 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Adipose tissue.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney.
[3]"Consortin, a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins."
del Castillo F.J., Cohen-Salmon M., Charollais A., Caille D., Lampe P.D., Chavrier P., Meda P., Petit C.
Hum. Mol. Genet. 19:262-275(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CNST.
[4]"Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis."
Makyio H., Ohgi M., Takei T., Takahashi S., Takatsu H., Katoh Y., Hanai A., Ueda T., Kanaho Y., Xie Y., Shin H.W., Kamikubo H., Kataoka M., Kawasaki M., Kato R., Wakatsuki S., Nakayama K.
EMBO J. 31:2590-2603(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARF6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK080881 mRNA. Translation: BAC38058.1.
AK155195 mRNA. Translation: BAE33109.1.
BC026802 mRNA. Translation: AAH26802.1.
CCDSCCDS27625.1.
RefSeqNP_666041.1. NM_145929.2.
UniGeneMm.251331.

3D structure databases

ProteinModelPortalQ8R0H9.
SMRQ8R0H9. Positions 2-147, 168-301, 495-635.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid222979. 2 interactions.
IntActQ8R0H9. 3 interactions.
MINTMINT-4118328.

PTM databases

PhosphoSiteQ8R0H9.

Proteomic databases

MaxQBQ8R0H9.
PaxDbQ8R0H9.
PRIDEQ8R0H9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000041587; ENSMUSP00000035992; ENSMUSG00000033128.
GeneID106039.
KEGGmmu:106039.
UCSCuc007wrn.2. mouse.

Organism-specific databases

CTD26088.
MGIMGI:2146207. Gga1.

Phylogenomic databases

eggNOGNOG321636.
GeneTreeENSGT00700000104396.
HOGENOMHOG000231169.
HOVERGENHBG015945.
InParanoidQ8R0H9.
KOK12404.
OMALTFTMGD.
OrthoDBEOG7V49Z0.
PhylomeDBQ8R0H9.
TreeFamTF318574.

Gene expression databases

BgeeQ8R0H9.
GenevestigatorQ8R0H9.

Family and domain databases

Gene3D1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view]
PfamPF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
SMARTSM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMSSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEPS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGGA1. mouse.
NextBio358026.
PROQ8R0H9.
SOURCESearch...

Entry information

Entry nameGGA1_MOUSE
AccessionPrimary (citable) accession number: Q8R0H9
Secondary accession number(s): Q3U2N1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot