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Q8R0H9

- GGA1_MOUSE

UniProt

Q8R0H9 - GGA1_MOUSE

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Protein

ADP-ribosylation factor-binding protein GGA1

Gene
Gga1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif By similarity.

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. intracellular protein transport Source: InterPro
  2. positive regulation of protein catabolic process Source: MGI
  3. vesicle-mediated transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-binding protein GGA1
Alternative name(s):
Gamma-adaptin-related protein 1
Golgi-localized, gamma ear-containing, ARF-binding protein 1
Gene namesi
Name:Gga1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:2146207. Gga1.

Subcellular locationi

GO - Cellular componenti

  1. clathrin adaptor complex Source: InterPro
  2. endosome membrane Source: UniProtKB-SubCell
  3. Golgi apparatus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 635635ADP-ribosylation factor-binding protein GGA1PRO_0000212681Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Post-translational modificationi

Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Ubiquitinated By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8R0H9.
PaxDbiQ8R0H9.
PRIDEiQ8R0H9.

PTM databases

PhosphoSiteiQ8R0H9.

Expressioni

Gene expression databases

BgeeiQ8R0H9.
GenevestigatoriQ8R0H9.

Interactioni

Subunit structurei

Monomer. Interacts with NECAP1 By similarity. Interacts with GGA2 and GGA3. Binds to clathrin and activated ARFs, including ARF1. Interacts with RABEP1 and RABGEF1. Interacts with the type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. Binds CCDC91, P200, SYNRG, EPN4, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and UBC By similarity. Interacts with RNF11 By similarity. Interacts with CNST. Interacts with ARF6.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CnstQ8CBC4-32EBI-2616212,EBI-2615407

Protein-protein interaction databases

BioGridi222979. 2 interactions.
IntActiQ8R0H9. 3 interactions.
MINTiMINT-4118328.

Structurei

3D structure databases

ProteinModelPortaliQ8R0H9.
SMRiQ8R0H9. Positions 2-147, 168-301, 495-635.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 147131VHSAdd
BLAST
Domaini171 – 298128GATAdd
BLAST
Domaini506 – 627122GAEAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni114 – 273160Interaction with ARF3 By similarityAdd
BLAST
Regioni299 – 505207Unstructured hingeAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi357 – 3615Autoinhibitory By similarity

Domaini

The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif) By similarity.
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis By similarity.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs By similarity.
The GAE domain binds accessory proteins regulating GGAs function.

Sequence similaritiesi

Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.

Phylogenomic databases

eggNOGiNOG321636.
GeneTreeiENSGT00700000104396.
HOGENOMiHOG000231169.
HOVERGENiHBG015945.
InParanoidiQ8R0H9.
KOiK12404.
OMAiLTFTMGD.
OrthoDBiEOG7V49Z0.
PhylomeDBiQ8R0H9.
TreeFamiTF318574.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view]
PfamiPF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R0H9-1 [UniParc]FASTAAdd to Basket

« Hide

MEPAMEPETL EARINRATNP LNKELNWASI NSFCEQLNED FEGPPLATRL    50
LAHKIQSPQE WEAIQALTVL ETCMKSCGKR FHDEVGKFRF LNELIKVVSP 100
KYLGSRTSEK VKSKILELLY SWTVCLPEEV KIAEAYQMLK KQGIVKSDPK 150
LPEDAIFPLP PPRPKNVIFE DEEKSKMLAR LLKSSHPEDL RAANKLIKEM 200
VQEDQKRMEK ISKRVNAIEE VNNNVKLLTE MVMSHSQGAA SSSSEDLMKE 250
LYQRCERMRP TLFRLASDTE DNDEALAEIL QANDNLTQVI NLYKQLVRGE 300
EVNGDATASS IPGSTSALLD LSGLDLPPPG TTQPATPTRP GNQSSPEQLS 350
ASVSLLDDEL MSLGLSDPTP PSGTSSDSVG WDNFQSSDGT ESSVPPPAQA 400
PSMDCRPPAQ APPPTSSGLD DLDLLGKTLM QQALPPEAQQ VRWEKQQPAP 450
RLTLRDLQSK SSSPSPGAAS LLHTTSPEPP GPPPQATPTE FSLTSITVPL 500
ESIKPSSILP VTVYDQHGFR VLFHFARDPL PGRSDVLVVV VSMLSTAPQP 550
IRNIVFQSAV PKVMKVRLQP PSGTELPAFN PIVHPSAITQ VLLLANPQKE 600
KVRLRYKLIF TMGDQTYNEM GDVDQFPPPE TWGSL 635
Length:635
Mass (Da):69,972
Last modified:June 1, 2002 - v1
Checksum:i9F5F1C37F41ECCC6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK080881 mRNA. Translation: BAC38058.1.
AK155195 mRNA. Translation: BAE33109.1.
BC026802 mRNA. Translation: AAH26802.1.
CCDSiCCDS27625.1.
RefSeqiNP_666041.1. NM_145929.2.
UniGeneiMm.251331.

Genome annotation databases

EnsembliENSMUST00000041587; ENSMUSP00000035992; ENSMUSG00000033128.
GeneIDi106039.
KEGGimmu:106039.
UCSCiuc007wrn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK080881 mRNA. Translation: BAC38058.1 .
AK155195 mRNA. Translation: BAE33109.1 .
BC026802 mRNA. Translation: AAH26802.1 .
CCDSi CCDS27625.1.
RefSeqi NP_666041.1. NM_145929.2.
UniGenei Mm.251331.

3D structure databases

ProteinModelPortali Q8R0H9.
SMRi Q8R0H9. Positions 2-147, 168-301, 495-635.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 222979. 2 interactions.
IntActi Q8R0H9. 3 interactions.
MINTi MINT-4118328.

PTM databases

PhosphoSitei Q8R0H9.

Proteomic databases

MaxQBi Q8R0H9.
PaxDbi Q8R0H9.
PRIDEi Q8R0H9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000041587 ; ENSMUSP00000035992 ; ENSMUSG00000033128 .
GeneIDi 106039.
KEGGi mmu:106039.
UCSCi uc007wrn.2. mouse.

Organism-specific databases

CTDi 26088.
MGIi MGI:2146207. Gga1.

Phylogenomic databases

eggNOGi NOG321636.
GeneTreei ENSGT00700000104396.
HOGENOMi HOG000231169.
HOVERGENi HBG015945.
InParanoidi Q8R0H9.
KOi K12404.
OMAi LTFTMGD.
OrthoDBi EOG7V49Z0.
PhylomeDBi Q8R0H9.
TreeFami TF318574.

Miscellaneous databases

ChiTaRSi GGA1. mouse.
NextBioi 358026.
PROi Q8R0H9.
SOURCEi Search...

Gene expression databases

Bgeei Q8R0H9.
Genevestigatori Q8R0H9.

Family and domain databases

Gene3Di 1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProi IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view ]
Pfami PF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view ]
SMARTi SM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view ]
SUPFAMi SSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEi PS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Adipose tissue.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  3. "Consortin, a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins."
    del Castillo F.J., Cohen-Salmon M., Charollais A., Caille D., Lampe P.D., Chavrier P., Meda P., Petit C.
    Hum. Mol. Genet. 19:262-275(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CNST.
  4. "Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis."
    Makyio H., Ohgi M., Takei T., Takahashi S., Takatsu H., Katoh Y., Hanai A., Ueda T., Kanaho Y., Xie Y., Shin H.W., Kamikubo H., Kataoka M., Kawasaki M., Kato R., Wakatsuki S., Nakayama K.
    EMBO J. 31:2590-2603(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARF6.

Entry informationi

Entry nameiGGA1_MOUSE
AccessioniPrimary (citable) accession number: Q8R0H9
Secondary accession number(s): Q3U2N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi