Reviewed, UniProtKB/Swiss-Prot Q8R0G7 (SPNS1_MOUSE)

Names and origin

Protein names

Recommended name:
    Protein spinster homolog 1

Gene names

Name:

Spns1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Sphingolipid transporter. May be involved in necrotic or autophagic cell death By similarity.
Subunit structure	Interacts with BCL2 and BCL2L1 By similarity.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the major facilitator superfamily. Spinster family.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	lipid transport Inferred from electronic annotation. Source: UniProtKB-KW transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 528	527	Protein spinster homolog 1		PRO_0000305040
Regions
	Transmembrane	60 – 80	21	Potential
	Transmembrane	98 – 118	21	Potential
	Transmembrane	126 – 146	21	Potential
	Transmembrane	160 – 180	21	Potential
	Transmembrane	187 – 207	21	Potential
	Transmembrane	218 – 238	21	Potential
	Transmembrane	278 – 298	21	Potential
	Transmembrane	323 – 343	21	Potential
	Transmembrane	357 – 377	21	Potential
	Transmembrane	381 – 401	21	Potential
	Transmembrane	421 – 441	21	Potential
	Transmembrane	465 – 485	21	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	518	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	60	1	L → I in BAE39125. Ref.2
	Sequence conflict	121	1	R → K in AAH02297. Ref.3
	Sequence conflict	127	1	L → F in AAG43831. Ref.1
	Sequence conflict	154	1	L → F in AAG43831. Ref.1
	Sequence conflict	159	1	L → M in AAG43831. Ref.1
	Sequence conflict	178	1	L → F in AAG43831. Ref.1
	Sequence conflict	341	1	V → M in AAG43831. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8R0G7-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: DDAB448D7D2B9E17	FASTA	528	56,709
	10 20 30 40 50 60 MAGSDTAPFL SQADDPDDGP APGHPGLPGP MGNPKSGELE VPDCEGLQRI TGLSRGHSTL 70 80 90 100 110 120 IVVVLCYINL LNYMDRFTVA GVLTDIEQFF NIGDGSTGLI QTVFISSYMV LAPVFGYLGD 130 140 150 160 170 180 RYNRKYLMCG GIAFWSLVTL GSSFIPREHF WLLLLTRGLV GVGEASYSTI APTLIADLFV 190 200 210 220 230 240 ADQRSRMLSI FYFAIPVGSG LGYIAGSKVK DVAGDWHWAL RVTPGLGVLA VLLLFLVVQE 250 260 270 280 290 300 PPRGAVERHS GSPPLSPTSW WADLKALARN PSFVLSSLGF TSVAFVTGSL ALWAPAFLLR 310 320 330 340 350 360 SRVVLGETPP CLPGDSCSSS DSLIFGLITC LTGVLGVGLG VEISRRLRRF NPRADPLVCA 370 380 390 400 410 420 AGLLGSAPFL FLALACARGS IVATYIFIFI GETLLSMNWA IVADILLYVV IPTRRSTAEA 430 440 450 460 470 480 FQIVLSHLLG DAGSPYLIGL ISDRLRRSWP PSFLSEFRAL QFSLMLCAFV GALGGAAFLG 490 500 510 520 TAMFIEDDRR RAQLHVQGLL HESGPSDDRI VVPQRGRSTR VPVSSVLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mutations in the novel membrane protein spinster interfere with programmed cell death and cause neural degeneration in Drosophila melanogaster." Nakano Y., Fujitani K., Kurihara J., Ragan J., Usui-Aoki K., Shimoda L., Lukacsovich T., Suzuki K., Sezaki M., Sano Y., Ueda R., Awano W., Kaneda M., Umeda M., Yamamoto D. Mol. Cell. Biol. 21:3775-3788(2001) [PubMed: 11340170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and Czech II. Tissue: Mammary tumor.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6 and FVB/N. Tissue: Brain and Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF212372 mRNA. Translation: AAG43831.1. AK166931 mRNA. Translation: BAE39125.1. BC002297 mRNA. Translation: AAH02297.1. BC026854 mRNA. Translation: AAH26854.1. BC085491 mRNA. Translation: AAH85491.1.
IPI	IPI00111254.
RefSeq	NP_076201.2.
UniGene	Mm.11112
3D structure databases
SMR	Q8R0G7. Positions 60-445.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8R0G7.
PTM databases
PhosphoSite	Q8R0G7.
Proteomic databases
PRIDE	Q8R0G7.
Genome annotation databases
Ensembl	ENSMUST00000032994; ENSMUSP00000032994; ENSMUSG00000030741; Mus musculus. [Genome view]
GeneID	73658.
KEGG	mmu:73658.
UCSC	uc009jqy.1. mouse.
Organism-specific databases
CTD	73658.
MGI	MGI:1920908. Spns1.
Phylogenomic databases
eggNOG	roNOG14264.
HOGENOM	HBG691938.
HOVERGEN	Q8R0G7.
InParanoid	Q8R0G7.
OMA	AQLHVQG.
PhylomeDB	Q8R0G7.
Gene expression databases
ArrayExpress	Q8R0G7.
Bgee	Q8R0G7.
CleanEx	MM_SPNS1.
Genevestigator	Q8R0G7.
Family and domain databases
InterPro	IPR011701. MFS_1. IPR016196. MFS_general_subst_transpt. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	338727.
SOURCE	Search...

Entry information

Entry name

SPNS1_MOUSE

Accession

Primary (citable) accession number: Q8R0G7
Secondary accession number(s): Q3TKM0, Q99LN7, Q9EQK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	June 1, 2002
Last modified:	January 19, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)