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Q8R0F6

- ILKAP_MOUSE

UniProt

Q8R0F6 - ILKAP_MOUSE

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Protein
Integrin-linked kinase-associated serine/threonine phosphatase 2C
Gene
Ilkap
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation By similarity.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi152 – 1521Manganese 1 By similarity
Metal bindingi152 – 1521Manganese 2 By similarity
Metal bindingi153 – 1531Manganese 1; via carbonyl oxygen By similarity
Metal bindingi326 – 3261Manganese 2 By similarity
Metal bindingi381 – 3811Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: Ensembl
Complete GO annotation...

GO - Biological processi

  1. protein dephosphorylation Source: Ensembl
  2. regulation of nuclear cell cycle DNA replication Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin-linked kinase-associated serine/threonine phosphatase 2C (EC:3.1.3.16)
Short name:
ILKAP
Gene namesi
Name:Ilkap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1914694. Ilkap.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Integrin-linked kinase-associated serine/threonine phosphatase 2C
PRO_0000272272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei210 – 2101N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8R0F6.
PaxDbiQ8R0F6.
PRIDEiQ8R0F6.

PTM databases

PhosphoSiteiQ8R0F6.

Expressioni

Gene expression databases

BgeeiQ8R0F6.
GenevestigatoriQ8R0F6.

Interactioni

Subunit structurei

Interacts with ILK By similarity.

Protein-protein interaction databases

BioGridi212191. 4 interactions.
IntActiQ8R0F6. 1 interaction.
MINTiMINT-4118288.

Structurei

3D structure databases

ProteinModelPortaliQ8R0F6.
SMRiQ8R0F6. Positions 116-388.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini128 – 390263PP2C-like
Add
BLAST

Sequence similaritiesi

Belongs to the PP2C family.
Contains 1 PP2C-like domain.

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000114971.
HOGENOMiHOG000233896.
HOVERGENiHBG054286.
InParanoidiQ8R0F6.
KOiK17500.
OMAiDIKRCQL.
OrthoDBiEOG7Z95NF.
PhylomeDBiQ8R0F6.
TreeFamiTF313513.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8R0F6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDLFGDLPEP ERAPRPSAGK EAQGRPVLFE DLPPASSTDS GSGGPLLFDD    50
LPPAASGNSG SLATSGSQVV KTEGKGAKRK APEEEKNGGE ELVEKKVCKA 100
SSVIFGLKGY VAERKGEREE MQDAHVILND ITQECNPPSS LITRVSYFAV 150
FDGHGGIRAS KFAAQNLHQN LIRKFPKGDI ISVEKTVKRC LLDTFKHTDE 200
EFLKQASSQK PAWKDGSTAT CVLAVDNILY IANLGDSRAI LCRYNEESQK 250
HAALSLSKEH NPTQYEERMR IQKAGGNVRD GRVLGVLEVS RSIGDGQYKR 300
CGVTSVPDIR RCQLTPNDRF ILLACDGLFK VFTPEEAVNF ILSCLEDDKI 350
QTREGKPAVD ARYEAACNRL ANKAVQRGSA DNVTVMVVRI GH 392
Length:392
Mass (Da):42,774
Last modified:June 1, 2002 - v1
Checksum:i8DE1F77DAC9B7176
GO
Isoform 2 (identifier: Q8R0F6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-120: Missing.
     347-392: DDKIQTREGK...VTVMVVRIGH → VPRQLHSSRS...VLGSLPEWMV

Show »
Length:269
Mass (Da):30,043
Checksum:iAE1F7F96B76FE5D5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 120120Missing in isoform 2.
VSP_022384Add
BLAST
Alternative sequencei347 – 39246DDKIQ…VRIGH → VPRQLHSSRSCFPEVSPERM TVVLAYTQLSISGVLGSLPE WMV in isoform 2.
VSP_022385Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC026953 mRNA. Translation: AAH26953.1.
BC027439 mRNA. Translation: AAH27439.1.
CCDSiCCDS15162.1. [Q8R0F6-1]
RefSeqiNP_075832.1. NM_023343.2. [Q8R0F6-1]
XP_006529879.1. XM_006529816.1. [Q8R0F6-2]
UniGeneiMm.337240.

Genome annotation databases

EnsembliENSMUST00000027534; ENSMUSP00000027534; ENSMUSG00000026309. [Q8R0F6-1]
GeneIDi67444.
KEGGimmu:67444.
UCSCiuc007cak.1. mouse. [Q8R0F6-2]
uc007cal.1. mouse. [Q8R0F6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC026953 mRNA. Translation: AAH26953.1 .
BC027439 mRNA. Translation: AAH27439.1 .
CCDSi CCDS15162.1. [Q8R0F6-1 ]
RefSeqi NP_075832.1. NM_023343.2. [Q8R0F6-1 ]
XP_006529879.1. XM_006529816.1. [Q8R0F6-2 ]
UniGenei Mm.337240.

3D structure databases

ProteinModelPortali Q8R0F6.
SMRi Q8R0F6. Positions 116-388.
ModBasei Search...

Protein-protein interaction databases

BioGridi 212191. 4 interactions.
IntActi Q8R0F6. 1 interaction.
MINTi MINT-4118288.

PTM databases

PhosphoSitei Q8R0F6.

Proteomic databases

MaxQBi Q8R0F6.
PaxDbi Q8R0F6.
PRIDEi Q8R0F6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027534 ; ENSMUSP00000027534 ; ENSMUSG00000026309 . [Q8R0F6-1 ]
GeneIDi 67444.
KEGGi mmu:67444.
UCSCi uc007cak.1. mouse. [Q8R0F6-2 ]
uc007cal.1. mouse. [Q8R0F6-1 ]

Organism-specific databases

CTDi 80895.
MGIi MGI:1914694. Ilkap.

Phylogenomic databases

eggNOGi COG0631.
GeneTreei ENSGT00740000114971.
HOGENOMi HOG000233896.
HOVERGENi HBG054286.
InParanoidi Q8R0F6.
KOi K17500.
OMAi DIKRCQL.
OrthoDBi EOG7Z95NF.
PhylomeDBi Q8R0F6.
TreeFami TF313513.

Miscellaneous databases

NextBioi 324586.
PROi Q8R0F6.
SOURCEi Search...

Gene expression databases

Bgeei Q8R0F6.
Genevestigatori Q8R0F6.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: FVB/N.
    Tissue: Liver.

Entry informationi

Entry nameiILKAP_MOUSE
AccessioniPrimary (citable) accession number: Q8R0F6
Secondary accession number(s): Q05CC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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