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Q8R0F6 (ILKAP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Integrin-linked kinase-associated serine/threonine phosphatase 2C
Short name=ILKAP
EC=3.1.3.16
Gene names
Name:Ilkap
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length392 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation By similarity.

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subunit structure

Interacts with ILK By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8R0F6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8R0F6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-120: Missing.
     347-392: DDKIQTREGK...VTVMVVRIGH → VPRQLHSSRS...VLGSLPEWMV

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 392392Integrin-linked kinase-associated serine/threonine phosphatase 2C
PRO_0000272272

Regions

Domain128 – 390263PP2C-like

Sites

Metal binding1521Manganese 1 By similarity
Metal binding1521Manganese 2 By similarity
Metal binding1531Manganese 1; via carbonyl oxygen By similarity
Metal binding3261Manganese 2 By similarity
Metal binding3811Manganese 2 By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue2101N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 120120Missing in isoform 2.
VSP_022384
Alternative sequence347 – 39246DDKIQ…VRIGH → VPRQLHSSRSCFPEVSPERM TVVLAYTQLSISGVLGSLPE WMV in isoform 2.
VSP_022385

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 8DE1F77DAC9B7176

FASTA39242,774
        10         20         30         40         50         60 
MDLFGDLPEP ERAPRPSAGK EAQGRPVLFE DLPPASSTDS GSGGPLLFDD LPPAASGNSG 

        70         80         90        100        110        120 
SLATSGSQVV KTEGKGAKRK APEEEKNGGE ELVEKKVCKA SSVIFGLKGY VAERKGEREE 

       130        140        150        160        170        180 
MQDAHVILND ITQECNPPSS LITRVSYFAV FDGHGGIRAS KFAAQNLHQN LIRKFPKGDI 

       190        200        210        220        230        240 
ISVEKTVKRC LLDTFKHTDE EFLKQASSQK PAWKDGSTAT CVLAVDNILY IANLGDSRAI 

       250        260        270        280        290        300 
LCRYNEESQK HAALSLSKEH NPTQYEERMR IQKAGGNVRD GRVLGVLEVS RSIGDGQYKR 

       310        320        330        340        350        360 
CGVTSVPDIR RCQLTPNDRF ILLACDGLFK VFTPEEAVNF ILSCLEDDKI QTREGKPAVD 

       370        380        390 
ARYEAACNRL ANKAVQRGSA DNVTVMVVRI GH

« Hide

Isoform 2 [UniParc].

Checksum: AE1F7F96B76FE5D5
Show »

FASTA26930,043

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: FVB/N.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC026953 mRNA. Translation: AAH26953.1.
BC027439 mRNA. Translation: AAH27439.1.
IPIIPI00396804.
IPI00828929.
RefSeqNP_075832.1. NM_023343.2.
UniGeneMm.337240.

3D structure databases

HSSPHSSP built from PDB template 1A6Q based on UniProtKB P35813.
ProteinModelPortalQ8R0F6.
SMRQ8R0F6. Positions 116-388.
ModBaseSearch...

PTM databases

PhosphoSiteQ8R0F6.

Proteomic databases

PaxDbQ8R0F6.
PRIDEQ8R0F6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027534; ENSMUSP00000027534; ENSMUSG00000026309.
GeneID67444.
KEGGmmu:67444.
UCSCuc007cak.1. mouse.
uc007cal.1. mouse.

Organism-specific databases

CTD80895.
MGIMGI:1914694. Ilkap.

Phylogenomic databases

eggNOGCOG0631.
GeneTreeENSGT00690000101775.
HOGENOMHOG000233896.
HOVERGENHBG054286.
InParanoidQ8R0F6.
OMADIKRCQL.
OrthoDBEOG4R503N.

Gene expression databases

BgeeQ8R0F6.
GenevestigatorQ8R0F6.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. PP2C-related. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio324586.
SOURCESearch...

Entry information

Entry nameILKAP_MOUSE
AccessionPrimary (citable) accession number: Q8R0F6
Secondary accession number(s): Q05CC3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: June 1, 2002
Last modified: May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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