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Q8R0F6

- ILKAP_MOUSE

UniProt

Q8R0F6 - ILKAP_MOUSE

Protein

Integrin-linked kinase-associated serine/threonine phosphatase 2C

Gene

Ilkap

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation By similarity.By similarity

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi152 – 1521Manganese 1By similarity
    Metal bindingi152 – 1521Manganese 2By similarity
    Metal bindingi153 – 1531Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi326 – 3261Manganese 2By similarity
    Metal bindingi381 – 3811Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: Ensembl

    GO - Biological processi

    1. protein dephosphorylation Source: Ensembl
    2. regulation of nuclear cell cycle DNA replication Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Integrin-linked kinase-associated serine/threonine phosphatase 2C (EC:3.1.3.16)
    Short name:
    ILKAP
    Gene namesi
    Name:Ilkap
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1914694. Ilkap.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 392392Integrin-linked kinase-associated serine/threonine phosphatase 2CPRO_0000272272Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei210 – 2101N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8R0F6.
    PaxDbiQ8R0F6.
    PRIDEiQ8R0F6.

    PTM databases

    PhosphoSiteiQ8R0F6.

    Expressioni

    Gene expression databases

    BgeeiQ8R0F6.
    GenevestigatoriQ8R0F6.

    Interactioni

    Subunit structurei

    Interacts with ILK.By similarity

    Protein-protein interaction databases

    BioGridi212191. 4 interactions.
    IntActiQ8R0F6. 1 interaction.
    MINTiMINT-4118288.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R0F6.
    SMRiQ8R0F6. Positions 116-388.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini128 – 390263PP2C-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    GeneTreeiENSGT00740000114971.
    HOGENOMiHOG000233896.
    HOVERGENiHBG054286.
    InParanoidiQ8R0F6.
    KOiK17500.
    OMAiDIKRCQL.
    OrthoDBiEOG7Z95NF.
    PhylomeDBiQ8R0F6.
    TreeFamiTF313513.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8R0F6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDLFGDLPEP ERAPRPSAGK EAQGRPVLFE DLPPASSTDS GSGGPLLFDD    50
    LPPAASGNSG SLATSGSQVV KTEGKGAKRK APEEEKNGGE ELVEKKVCKA 100
    SSVIFGLKGY VAERKGEREE MQDAHVILND ITQECNPPSS LITRVSYFAV 150
    FDGHGGIRAS KFAAQNLHQN LIRKFPKGDI ISVEKTVKRC LLDTFKHTDE 200
    EFLKQASSQK PAWKDGSTAT CVLAVDNILY IANLGDSRAI LCRYNEESQK 250
    HAALSLSKEH NPTQYEERMR IQKAGGNVRD GRVLGVLEVS RSIGDGQYKR 300
    CGVTSVPDIR RCQLTPNDRF ILLACDGLFK VFTPEEAVNF ILSCLEDDKI 350
    QTREGKPAVD ARYEAACNRL ANKAVQRGSA DNVTVMVVRI GH 392
    Length:392
    Mass (Da):42,774
    Last modified:June 1, 2002 - v1
    Checksum:i8DE1F77DAC9B7176
    GO
    Isoform 2 (identifier: Q8R0F6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-120: Missing.
         347-392: DDKIQTREGK...VTVMVVRIGH → VPRQLHSSRS...VLGSLPEWMV

    Show »
    Length:269
    Mass (Da):30,043
    Checksum:iAE1F7F96B76FE5D5
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 120120Missing in isoform 2. 1 PublicationVSP_022384Add
    BLAST
    Alternative sequencei347 – 39246DDKIQ…VRIGH → VPRQLHSSRSCFPEVSPERM TVVLAYTQLSISGVLGSLPE WMV in isoform 2. 1 PublicationVSP_022385Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC026953 mRNA. Translation: AAH26953.1.
    BC027439 mRNA. Translation: AAH27439.1.
    CCDSiCCDS15162.1. [Q8R0F6-1]
    RefSeqiNP_075832.1. NM_023343.2. [Q8R0F6-1]
    XP_006529879.1. XM_006529816.1. [Q8R0F6-2]
    UniGeneiMm.337240.

    Genome annotation databases

    EnsembliENSMUST00000027534; ENSMUSP00000027534; ENSMUSG00000026309. [Q8R0F6-1]
    GeneIDi67444.
    KEGGimmu:67444.
    UCSCiuc007cak.1. mouse. [Q8R0F6-2]
    uc007cal.1. mouse. [Q8R0F6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC026953 mRNA. Translation: AAH26953.1 .
    BC027439 mRNA. Translation: AAH27439.1 .
    CCDSi CCDS15162.1. [Q8R0F6-1 ]
    RefSeqi NP_075832.1. NM_023343.2. [Q8R0F6-1 ]
    XP_006529879.1. XM_006529816.1. [Q8R0F6-2 ]
    UniGenei Mm.337240.

    3D structure databases

    ProteinModelPortali Q8R0F6.
    SMRi Q8R0F6. Positions 116-388.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 212191. 4 interactions.
    IntActi Q8R0F6. 1 interaction.
    MINTi MINT-4118288.

    PTM databases

    PhosphoSitei Q8R0F6.

    Proteomic databases

    MaxQBi Q8R0F6.
    PaxDbi Q8R0F6.
    PRIDEi Q8R0F6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027534 ; ENSMUSP00000027534 ; ENSMUSG00000026309 . [Q8R0F6-1 ]
    GeneIDi 67444.
    KEGGi mmu:67444.
    UCSCi uc007cak.1. mouse. [Q8R0F6-2 ]
    uc007cal.1. mouse. [Q8R0F6-1 ]

    Organism-specific databases

    CTDi 80895.
    MGIi MGI:1914694. Ilkap.

    Phylogenomic databases

    eggNOGi COG0631.
    GeneTreei ENSGT00740000114971.
    HOGENOMi HOG000233896.
    HOVERGENi HBG054286.
    InParanoidi Q8R0F6.
    KOi K17500.
    OMAi DIKRCQL.
    OrthoDBi EOG7Z95NF.
    PhylomeDBi Q8R0F6.
    TreeFami TF313513.

    Miscellaneous databases

    NextBioi 324586.
    PROi Q8R0F6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8R0F6.
    Genevestigatori Q8R0F6.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: FVB/N.
      Tissue: Liver.

    Entry informationi

    Entry nameiILKAP_MOUSE
    AccessioniPrimary (citable) accession number: Q8R0F6
    Secondary accession number(s): Q05CC3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3