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Protein

Integrin-linked kinase-associated serine/threonine phosphatase 2C

Gene

Ilkap

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi152 – 1521Manganese 1By similarity
Metal bindingi152 – 1521Manganese 2By similarity
Metal bindingi153 – 1531Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi326 – 3261Manganese 2By similarity
Metal bindingi381 – 3811Manganese 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin-linked kinase-associated serine/threonine phosphatase 2C (EC:3.1.3.16)
Short name:
ILKAP
Gene namesi
Name:Ilkap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1914694. Ilkap.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Integrin-linked kinase-associated serine/threonine phosphatase 2CPRO_0000272272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei210 – 2101N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8R0F6.
PaxDbiQ8R0F6.
PRIDEiQ8R0F6.

PTM databases

PhosphoSiteiQ8R0F6.

Expressioni

Gene expression databases

BgeeiQ8R0F6.
GenevisibleiQ8R0F6. MM.

Interactioni

Subunit structurei

Interacts with ILK.By similarity

Protein-protein interaction databases

BioGridi212191. 4 interactions.
IntActiQ8R0F6. 1 interaction.
MINTiMINT-4118288.
STRINGi10090.ENSMUSP00000027534.

Structurei

3D structure databases

ProteinModelPortaliQ8R0F6.
SMRiQ8R0F6. Positions 116-388.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini108 – 390283PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000114971.
HOGENOMiHOG000233896.
HOVERGENiHBG054286.
InParanoidiQ8R0F6.
KOiK17500.
OMAiPDIKRCQ.
OrthoDBiEOG7Z95NF.
PhylomeDBiQ8R0F6.
TreeFamiTF313513.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R0F6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLFGDLPEP ERAPRPSAGK EAQGRPVLFE DLPPASSTDS GSGGPLLFDD
60 70 80 90 100
LPPAASGNSG SLATSGSQVV KTEGKGAKRK APEEEKNGGE ELVEKKVCKA
110 120 130 140 150
SSVIFGLKGY VAERKGEREE MQDAHVILND ITQECNPPSS LITRVSYFAV
160 170 180 190 200
FDGHGGIRAS KFAAQNLHQN LIRKFPKGDI ISVEKTVKRC LLDTFKHTDE
210 220 230 240 250
EFLKQASSQK PAWKDGSTAT CVLAVDNILY IANLGDSRAI LCRYNEESQK
260 270 280 290 300
HAALSLSKEH NPTQYEERMR IQKAGGNVRD GRVLGVLEVS RSIGDGQYKR
310 320 330 340 350
CGVTSVPDIR RCQLTPNDRF ILLACDGLFK VFTPEEAVNF ILSCLEDDKI
360 370 380 390
QTREGKPAVD ARYEAACNRL ANKAVQRGSA DNVTVMVVRI GH
Length:392
Mass (Da):42,774
Last modified:June 1, 2002 - v1
Checksum:i8DE1F77DAC9B7176
GO
Isoform 2 (identifier: Q8R0F6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-120: Missing.
     347-392: DDKIQTREGK...VTVMVVRIGH → VPRQLHSSRS...VLGSLPEWMV

Show »
Length:269
Mass (Da):30,043
Checksum:iAE1F7F96B76FE5D5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 120120Missing in isoform 2. 1 PublicationVSP_022384Add
BLAST
Alternative sequencei347 – 39246DDKIQ…VRIGH → VPRQLHSSRSCFPEVSPERM TVVLAYTQLSISGVLGSLPE WMV in isoform 2. 1 PublicationVSP_022385Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026953 mRNA. Translation: AAH26953.1.
BC027439 mRNA. Translation: AAH27439.1.
CCDSiCCDS15162.1. [Q8R0F6-1]
RefSeqiNP_075832.1. NM_023343.2. [Q8R0F6-1]
XP_006529879.1. XM_006529816.2. [Q8R0F6-2]
UniGeneiMm.337240.

Genome annotation databases

EnsembliENSMUST00000027534; ENSMUSP00000027534; ENSMUSG00000026309. [Q8R0F6-1]
GeneIDi67444.
KEGGimmu:67444.
UCSCiuc007cak.1. mouse. [Q8R0F6-2]
uc007cal.1. mouse. [Q8R0F6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026953 mRNA. Translation: AAH26953.1.
BC027439 mRNA. Translation: AAH27439.1.
CCDSiCCDS15162.1. [Q8R0F6-1]
RefSeqiNP_075832.1. NM_023343.2. [Q8R0F6-1]
XP_006529879.1. XM_006529816.2. [Q8R0F6-2]
UniGeneiMm.337240.

3D structure databases

ProteinModelPortaliQ8R0F6.
SMRiQ8R0F6. Positions 116-388.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212191. 4 interactions.
IntActiQ8R0F6. 1 interaction.
MINTiMINT-4118288.
STRINGi10090.ENSMUSP00000027534.

PTM databases

PhosphoSiteiQ8R0F6.

Proteomic databases

MaxQBiQ8R0F6.
PaxDbiQ8R0F6.
PRIDEiQ8R0F6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027534; ENSMUSP00000027534; ENSMUSG00000026309. [Q8R0F6-1]
GeneIDi67444.
KEGGimmu:67444.
UCSCiuc007cak.1. mouse. [Q8R0F6-2]
uc007cal.1. mouse. [Q8R0F6-1]

Organism-specific databases

CTDi80895.
MGIiMGI:1914694. Ilkap.

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000114971.
HOGENOMiHOG000233896.
HOVERGENiHBG054286.
InParanoidiQ8R0F6.
KOiK17500.
OMAiPDIKRCQ.
OrthoDBiEOG7Z95NF.
PhylomeDBiQ8R0F6.
TreeFamiTF313513.

Miscellaneous databases

ChiTaRSiIlkap. mouse.
NextBioi324586.
PROiQ8R0F6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R0F6.
GenevisibleiQ8R0F6. MM.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: FVB/N.
    Tissue: Liver.

Entry informationi

Entry nameiILKAP_MOUSE
AccessioniPrimary (citable) accession number: Q8R0F6
Secondary accession number(s): Q05CC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: June 1, 2002
Last modified: June 24, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.