Reviewed, UniProtKB/Swiss-Prot Q8R087 (B4GT7_MOUSE)

Names and origin

Protein names

Recommended name:
    Beta-1,4-galactosyltransferase 7
      Short name=Beta-1,4-GalTase 7
      Short name=Beta4Gal-T7
      Short name=b4Gal-T7
    EC=2.4.1.-
Alternative name(s):
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 7
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 7
Including the following 1 domains:
    1- Recommended name:
            Xylosylprotein 4-beta-galactosyltransferase
              EC=2.4.1.133
        Alternative name(s):
            UDP-galactose:beta-xylose beta-1,4-galactosyltransferase
            Xylosylprotein beta-1,4-galactosyltransferase
            XGPT
            Proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I
            XGalT-1

Gene names

Name:	B4galt7
Synonyms:	Xgalt1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Required for the biosynthesis of the tetrasaccharide linkage region of proteoglycans, especially for small proteoglycans in skin fibroblasts By similarity.
Catalytic activity	UDP-galactose + O-beta-D-xylosylprotein = UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Cis cisternae of Golgi stack By similarity.
Sequence similarities	Belongs to the glycosyltransferase 7 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	fibril organization Inferred from sequence or structural similarity. Source: UniProtKB glycosaminoglycan biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of fibroblast proliferation Inferred from sequence or structural similarity. Source: UniProtKB proteoglycan metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW xylosylprotein 4-beta-galactosyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8R087-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8R087-2)

The sequence of this isoform differs from the canonical sequence as follows:
     241-275: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Beta-1,4-galactosyltransferase 7		PRO_0000080551
Regions
	Topological domain	1 – 30	30	Cytoplasmic Potential
	Transmembrane	31 – 51	21	Signal-anchor for type II membrane protein Potential
	Topological domain	52 – 327	276	Lumenal Potential
Sites
	Metal binding	165	1	Manganese By similarity
	Metal binding	257	1	Manganese By similarity
Amino acid modifications
	Glycosylation	154	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	241 – 275	35	Missing in isoform 2.		VSP_014231

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F79B656325DCFDE0	FASTA	327	37,754
	10 20 30 40 50 60 MLPSRRKAAQ LPWEDGRARL LPGGLRRKCS IFHLFIAFLL LVFFSLLWLQ LSCSGDMAQV 70 80 90 100 110 120 TRGQGQETSG PPRACPPEPP PEHWEEDESW GPHRLAVLVP FRERFEELLV FVPHMHRFLS 130 140 150 160 170 180 RKRIQHHIYV LNQVDHFRFN RAALINVGFL ESSNSTDYIA MHDVDLLPLN EELDYGFPEA 190 200 210 220 230 240 GPFHVASPEL HPLYHYKTYV GGILLLSKQH YQLCNGMSNR FWGWGREDDE FYRRIKGAGL 250 260 270 280 290 300 QLFRPSGITT GYQTFRHLHD PAWRKRDQKR IAAQKQEQFK VDREGGLNTV KYRVDSRTAL 310 320 SIGGAPCTVL NVMLDCDKTA TPWCIFG
	Isoform 2. Checksum: D50E046DBEF4D599	FASTA	292	33,563
	10 20 30 40 50 60 MLPSRRKAAQ LPWEDGRARL LPGGLRRKCS IFHLFIAFLL LVFFSLLWLQ LSCSGDMAQV 70 80 90 100 110 120 TRGQGQETSG PPRACPPEPP PEHWEEDESW GPHRLAVLVP FRERFEELLV FVPHMHRFLS 130 140 150 160 170 180 RKRIQHHIYV LNQVDHFRFN RAALINVGFL ESSNSTDYIA MHDVDLLPLN EELDYGFPEA 190 200 210 220 230 240 GPFHVASPEL HPLYHYKTYV GGILLLSKQH YQLCNGMSNR FWGWGREDDE FYRRIKGAGL 250 260 270 280 290 QEQFKVDREG GLNTVKYRVD SRTALSIGGA PCTVLNVMLD CDKTATPWCI FG

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: FVB/N. Tissue: Brain and Colon.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

b4GalT7

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC027195 mRNA. Translation: AAH27195.1. BC055703 mRNA. Translation: AAH55703.1.
IPI	IPI00153145. IPI00408006.
RefSeq	NP_666157.1.
UniGene	Mm.139825
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8R087.
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
Proteomic databases
PRIDE	Q8R087.
Genome annotation databases
Ensembl	ENSMUST00000064701; ENSMUSP00000068532; ENSMUSG00000021504; Mus musculus. [Genome view] ENSMUST00000100764; ENSMUSP00000098327; ENSMUSG00000021504; Mus musculus. [Genome view]
GeneID	218271.
KEGG	mmu:218271.
UCSC	uc007qru.1. mouse.
Organism-specific databases
CTD	218271.
MGI	MGI:2384987. B4galt7.
Phylogenomic databases
HOGENOM	HBG315803.
HOVERGEN	Q8R087.
InParanoid	Q8R087.
OMA	GWGLEDD.
OrthoDB	EOG9BZQNC.
PhylomeDB	Q8R087.
Enzyme and pathway databases
BRENDA	2.4.1.133. 244.
Gene expression databases
ArrayExpress	Q8R087.
Bgee	Q8R087.
Genevestigator	Q8R087.
GermOnline	ENSMUSG00000021504. Mus musculus.
Family and domain databases
InterPro	IPR003859. Galactosyl_T_2. [Graphical view]
PANTHER	PTHR19300. Galactosyl_T_2. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	376224.
SOURCE	Search...

Entry information

Entry name

B4GT7_MOUSE

Accession

Primary (citable) accession number: Q8R087
Secondary accession number(s): Q7TNU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)