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Q8R087 (B4GT7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,4-galactosyltransferase 7

Short name=Beta-1,4-GalTase 7
Short name=Beta4Gal-T7
Short name=b4Gal-T7
EC=2.4.1.-
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 7
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 7

Including the following 1 domains:

  1. Xylosylprotein 4-beta-galactosyltransferase
    EC=2.4.1.133
    Alternative name(s):
    Proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I
    UDP-galactose:beta-xylose beta-1,4-galactosyltransferase
    XGPT
    XGalT-1
    Xylosylprotein beta-1,4-galactosyltransferase
Gene names
Name:B4galt7
Synonyms:Xgalt1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for the biosynthesis of the tetrasaccharide linkage region of proteoglycans, especially for small proteoglycans in skin fibroblasts By similarity.

Catalytic activity

UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein] = UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein].

Cofactor

Manganese By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity. Note: Cis cisternae of Golgi stack By similarity.

Sequence similarities

Belongs to the glycosyltransferase 7 family.

Ontologies

Keywords
   Cellular componentGolgi apparatus
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   LigandManganese
Metal-binding
   Molecular functionGlycosyltransferase
Transferase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processextracellular fibril organization

Inferred from sequence or structural similarity. Source: UniProtKB

glycosaminoglycan biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of fibroblast proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

protein N-linked glycosylation

Inferred from sequence or structural similarity. Source: UniProtKB

proteoglycan metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentGolgi apparatus

Inferred from sequence or structural similarity. Source: UniProtKB

Golgi cisterna membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionbeta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity

Inferred from electronic annotation. Source: Ensembl

galactosyltransferase activity

Inferred from sequence or structural similarity. Source: UniProtKB

manganese ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

xylosylprotein 4-beta-galactosyltransferase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8R087-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8R087-2)

The sequence of this isoform differs from the canonical sequence as follows:
     241-275: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 327327Beta-1,4-galactosyltransferase 7
PRO_0000080551

Regions

Topological domain1 – 3030Cytoplasmic Potential
Transmembrane31 – 5121Helical; Signal-anchor for type II membrane protein; Potential
Topological domain52 – 327276Lumenal Potential
Region100 – 1045UDP-alpha-D-galactose binding By similarity
Region139 – 1413UDP-alpha-D-galactose binding By similarity
Region164 – 1652UDP-alpha-D-galactose binding By similarity
Region226 – 2294N-acetyl-D-glucosamine binding By similarity
Region257 – 2593UDP-alpha-D-galactose binding By similarity

Sites

Metal binding1651Manganese By similarity
Metal binding2571Manganese; via tele nitrogen By similarity
Binding site1941UDP-alpha-D-galactose By similarity
Binding site2241UDP-alpha-D-galactose By similarity
Binding site2661UDP-alpha-D-galactose By similarity

Amino acid modifications

Glycosylation1541N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence241 – 27535Missing in isoform 2.
VSP_014231

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: F79B656325DCFDE0

FASTA32737,754
        10         20         30         40         50         60 
MLPSRRKAAQ LPWEDGRARL LPGGLRRKCS IFHLFIAFLL LVFFSLLWLQ LSCSGDMAQV 

        70         80         90        100        110        120 
TRGQGQETSG PPRACPPEPP PEHWEEDESW GPHRLAVLVP FRERFEELLV FVPHMHRFLS 

       130        140        150        160        170        180 
RKRIQHHIYV LNQVDHFRFN RAALINVGFL ESSNSTDYIA MHDVDLLPLN EELDYGFPEA 

       190        200        210        220        230        240 
GPFHVASPEL HPLYHYKTYV GGILLLSKQH YQLCNGMSNR FWGWGREDDE FYRRIKGAGL 

       250        260        270        280        290        300 
QLFRPSGITT GYQTFRHLHD PAWRKRDQKR IAAQKQEQFK VDREGGLNTV KYRVDSRTAL 

       310        320 
SIGGAPCTVL NVMLDCDKTA TPWCIFG 

« Hide

Isoform 2 [UniParc].

Checksum: D50E046DBEF4D599
Show »

FASTA29233,563

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: FVB/N.
Tissue: Brain and Colon.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC027195 mRNA. Translation: AAH27195.1.
BC055703 mRNA. Translation: AAH55703.1.
CCDSCCDS26550.1. [Q8R087-1]
RefSeqNP_666157.1. NM_146045.1. [Q8R087-1]
XP_006517279.1. XM_006517216.1. [Q8R087-2]
UniGeneMm.139825.

3D structure databases

ProteinModelPortalQ8R087.
SMRQ8R087. Positions 81-326.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4997781.

Protein family/group databases

CAZyGT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSiteQ8R087.

Proteomic databases

PRIDEQ8R087.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000064701; ENSMUSP00000068532; ENSMUSG00000021504. [Q8R087-1]
ENSMUST00000100764; ENSMUSP00000098327; ENSMUSG00000021504. [Q8R087-2]
GeneID218271.
KEGGmmu:218271.
UCSCuc007qru.1. mouse. [Q8R087-1]
uc011yzw.1. mouse. [Q8R087-2]

Organism-specific databases

CTD11285.
MGIMGI:2384987. B4galt7.

Phylogenomic databases

eggNOGNOG305756.
GeneTreeENSGT00750000117412.
HOGENOMHOG000286021.
HOVERGENHBG050654.
InParanoidQ8R087.
KOK00733.
OMAFRFNRAS.
OrthoDBEOG7C5M8P.
PhylomeDBQ8R087.
TreeFamTF312834.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

ArrayExpressQ8R087.
BgeeQ8R087.
GenevestigatorQ8R087.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERPTHR19300. PTHR19300. 1 hit.
PfamPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSPR02050. B14GALTRFASE.
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Other

NextBio376224.
PROQ8R087.
SOURCESearch...

Entry information

Entry nameB4GT7_MOUSE
AccessionPrimary (citable) accession number: Q8R087
Secondary accession number(s): Q7TNU3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot