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Q8R087

- B4GT7_MOUSE

UniProt

Q8R087 - B4GT7_MOUSE

Protein

Beta-1,4-galactosyltransferase 7

Gene

B4galt7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Required for the biosynthesis of the tetrasaccharide linkage region of proteoglycans, especially for small proteoglycans in skin fibroblasts.By similarity

    Catalytic activityi

    UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein] = UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein].

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi165 – 1651ManganeseBy similarity
    Binding sitei194 – 1941UDP-alpha-D-galactoseBy similarity
    Binding sitei224 – 2241UDP-alpha-D-galactoseBy similarity
    Metal bindingi257 – 2571Manganese; via tele nitrogenBy similarity
    Binding sitei266 – 2661UDP-alpha-D-galactoseBy similarity

    GO - Molecular functioni

    1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: Ensembl
    2. galactosyltransferase activity Source: UniProtKB
    3. manganese ion binding Source: UniProtKB
    4. xylosylprotein 4-beta-galactosyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. extracellular fibril organization Source: UniProtKB
    2. glycosaminoglycan biosynthetic process Source: UniProtKB
    3. negative regulation of fibroblast proliferation Source: UniProtKB
    4. protein N-linked glycosylation Source: UniProtKB
    5. proteoglycan metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196489. A tetrasaccharide linker sequence is required for GAG synthesis.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 7 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 7
    Short name:
    Beta4Gal-T7
    Short name:
    b4Gal-T7
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 7
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 7
    Including the following 1 domains:
    Xylosylprotein 4-beta-galactosyltransferase (EC:2.4.1.133)
    Alternative name(s):
    Proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I
    UDP-galactose:beta-xylose beta-1,4-galactosyltransferase
    XGPT
    XGalT-1
    Xylosylprotein beta-1,4-galactosyltransferase
    Gene namesi
    Name:B4galt7
    Synonyms:Xgalt1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:2384987. B4galt7.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Cis cisternae of Golgi stack.By similarity

    GO - Cellular componenti

    1. Golgi apparatus Source: UniProtKB
    2. Golgi cisterna membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327Beta-1,4-galactosyltransferase 7PRO_0000080551Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ8R087.

    PTM databases

    PhosphoSiteiQ8R087.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8R087.
    BgeeiQ8R087.
    GenevestigatoriQ8R087.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4997781.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R087.
    SMRiQ8R087. Positions 81-326.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3030CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini52 – 327276LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei31 – 5121Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni100 – 1045UDP-alpha-D-galactose bindingBy similarity
    Regioni139 – 1413UDP-alpha-D-galactose bindingBy similarity
    Regioni164 – 1652UDP-alpha-D-galactose bindingBy similarity
    Regioni226 – 2294N-acetyl-D-glucosamine bindingBy similarity
    Regioni257 – 2593UDP-alpha-D-galactose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG305756.
    GeneTreeiENSGT00750000117412.
    HOGENOMiHOG000286021.
    HOVERGENiHBG050654.
    InParanoidiQ8R087.
    KOiK00733.
    OMAiFRFNRAS.
    OrthoDBiEOG7C5M8P.
    PhylomeDBiQ8R087.
    TreeFamiTF312834.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8R087-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLPSRRKAAQ LPWEDGRARL LPGGLRRKCS IFHLFIAFLL LVFFSLLWLQ    50
    LSCSGDMAQV TRGQGQETSG PPRACPPEPP PEHWEEDESW GPHRLAVLVP 100
    FRERFEELLV FVPHMHRFLS RKRIQHHIYV LNQVDHFRFN RAALINVGFL 150
    ESSNSTDYIA MHDVDLLPLN EELDYGFPEA GPFHVASPEL HPLYHYKTYV 200
    GGILLLSKQH YQLCNGMSNR FWGWGREDDE FYRRIKGAGL QLFRPSGITT 250
    GYQTFRHLHD PAWRKRDQKR IAAQKQEQFK VDREGGLNTV KYRVDSRTAL 300
    SIGGAPCTVL NVMLDCDKTA TPWCIFG 327
    Length:327
    Mass (Da):37,754
    Last modified:June 1, 2002 - v1
    Checksum:iF79B656325DCFDE0
    GO
    Isoform 2 (identifier: Q8R087-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         241-275: Missing.

    Show »
    Length:292
    Mass (Da):33,563
    Checksum:iD50E046DBEF4D599
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei241 – 27535Missing in isoform 2. 1 PublicationVSP_014231Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC027195 mRNA. Translation: AAH27195.1.
    BC055703 mRNA. Translation: AAH55703.1.
    CCDSiCCDS26550.1. [Q8R087-1]
    RefSeqiNP_666157.1. NM_146045.1. [Q8R087-1]
    XP_006517279.1. XM_006517216.1. [Q8R087-2]
    UniGeneiMm.139825.

    Genome annotation databases

    EnsembliENSMUST00000064701; ENSMUSP00000068532; ENSMUSG00000021504. [Q8R087-1]
    ENSMUST00000100764; ENSMUSP00000098327; ENSMUSG00000021504. [Q8R087-2]
    GeneIDi218271.
    KEGGimmu:218271.
    UCSCiuc007qru.1. mouse. [Q8R087-1]
    uc011yzw.1. mouse. [Q8R087-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    b4GalT7

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC027195 mRNA. Translation: AAH27195.1 .
    BC055703 mRNA. Translation: AAH55703.1 .
    CCDSi CCDS26550.1. [Q8R087-1 ]
    RefSeqi NP_666157.1. NM_146045.1. [Q8R087-1 ]
    XP_006517279.1. XM_006517216.1. [Q8R087-2 ]
    UniGenei Mm.139825.

    3D structure databases

    ProteinModelPortali Q8R087.
    SMRi Q8R087. Positions 81-326.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4997781.

    Protein family/group databases

    CAZyi GT7. Glycosyltransferase Family 7.

    PTM databases

    PhosphoSitei Q8R087.

    Proteomic databases

    PRIDEi Q8R087.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000064701 ; ENSMUSP00000068532 ; ENSMUSG00000021504 . [Q8R087-1 ]
    ENSMUST00000100764 ; ENSMUSP00000098327 ; ENSMUSG00000021504 . [Q8R087-2 ]
    GeneIDi 218271.
    KEGGi mmu:218271.
    UCSCi uc007qru.1. mouse. [Q8R087-1 ]
    uc011yzw.1. mouse. [Q8R087-2 ]

    Organism-specific databases

    CTDi 11285.
    MGIi MGI:2384987. B4galt7.

    Phylogenomic databases

    eggNOGi NOG305756.
    GeneTreei ENSGT00750000117412.
    HOGENOMi HOG000286021.
    HOVERGENi HBG050654.
    InParanoidi Q8R087.
    KOi K00733.
    OMAi FRFNRAS.
    OrthoDBi EOG7C5M8P.
    PhylomeDBi Q8R087.
    TreeFami TF312834.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_196489. A tetrasaccharide linker sequence is required for GAG synthesis.

    Miscellaneous databases

    NextBioi 376224.
    PROi Q8R087.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8R087.
    Bgeei Q8R087.
    Genevestigatori Q8R087.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR19300. PTHR19300. 1 hit.
    Pfami PF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view ]
    PRINTSi PR02050. B14GALTRFASE.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: FVB/N.
      Tissue: Brain and Colon.

    Entry informationi

    Entry nameiB4GT7_MOUSE
    AccessioniPrimary (citable) accession number: Q8R087
    Secondary accession number(s): Q7TNU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3