Q8R087 (B4GT7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,4-galactosyltransferase 7
Short name=Beta-1,4-GalTase 7
Short name=Beta4Gal-T7
Short name=b4Gal-T7
EC=2.4.1.-

Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 7
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 7

Including the following 1 domains:

1. Xylosylprotein 4-beta-galactosyltransferase
   EC=2.4.1.133
   Alternative name(s):
   Proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I
   UDP-galactose:beta-xylose beta-1,4-galactosyltransferase
   XGPT
   XGalT-1
   Xylosylprotein beta-1,4-galactosyltransferase

Gene names

Name:	B4galt7
Synonyms:	Xgalt1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for the biosynthesis of the tetrasaccharide linkage region of proteoglycans, especially for small proteoglycans in skin fibroblasts By similarity.
Catalytic activity	UDP-galactose + O-beta-D-xylosylprotein = UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Cis cisternae of Golgi stack By similarity.
Sequence similarities	Belongs to the glycosyltransferase 7 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fibril organization Inferred from sequence or structural similarity. Source: UniProtKB glycosaminoglycan biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of fibroblast proliferation Inferred from sequence or structural similarity. Source: UniProtKB proteoglycan metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW xylosylprotein 4-beta-galactosyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8R087-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8R087-2)

The sequence of this isoform differs from the canonical sequence as follows:
     241-275: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Beta-1,4-galactosyltransferase 7		PRO_0000080551
Regions
	Topological domain	1 – 30	30	Cytoplasmic Potential
	Transmembrane	31 – 51	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	52 – 327	276	Lumenal Potential
Sites
	Metal binding	165	1	Manganese By similarity
	Metal binding	257	1	Manganese By similarity
Amino acid modifications
	Glycosylation	154	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	241 – 275	35	Missing in isoform 2.		VSP_014231

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F79B656325DCFDE0	FASTA	327	37,754
	10 20 30 40 50 60 MLPSRRKAAQ LPWEDGRARL LPGGLRRKCS IFHLFIAFLL LVFFSLLWLQ LSCSGDMAQV 70 80 90 100 110 120 TRGQGQETSG PPRACPPEPP PEHWEEDESW GPHRLAVLVP FRERFEELLV FVPHMHRFLS 130 140 150 160 170 180 RKRIQHHIYV LNQVDHFRFN RAALINVGFL ESSNSTDYIA MHDVDLLPLN EELDYGFPEA 190 200 210 220 230 240 GPFHVASPEL HPLYHYKTYV GGILLLSKQH YQLCNGMSNR FWGWGREDDE FYRRIKGAGL 250 260 270 280 290 300 QLFRPSGITT GYQTFRHLHD PAWRKRDQKR IAAQKQEQFK VDREGGLNTV KYRVDSRTAL 310 320 SIGGAPCTVL NVMLDCDKTA TPWCIFG
	Isoform 2 [UniParc]. Checksum: D50E046DBEF4D599	FASTA	292	33,563
	10 20 30 40 50 60 MLPSRRKAAQ LPWEDGRARL LPGGLRRKCS IFHLFIAFLL LVFFSLLWLQ LSCSGDMAQV 70 80 90 100 110 120 TRGQGQETSG PPRACPPEPP PEHWEEDESW GPHRLAVLVP FRERFEELLV FVPHMHRFLS 130 140 150 160 170 180 RKRIQHHIYV LNQVDHFRFN RAALINVGFL ESSNSTDYIA MHDVDLLPLN EELDYGFPEA 190 200 210 220 230 240 GPFHVASPEL HPLYHYKTYV GGILLLSKQH YQLCNGMSNR FWGWGREDDE FYRRIKGAGL 250 260 270 280 290 QEQFKVDREG GLNTVKYRVD SRTALSIGGA PCTVLNVMLD CDKTATPWCI FG

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: FVB/N. Tissue: Brain and Colon.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

b4GalT7

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC027195 mRNA. Translation: AAH27195.1. BC055703 mRNA. Translation: AAH55703.1.
IPI	IPI00153145. IPI00408006.
RefSeq	NP_666157.1. NM_146045.1.
UniGene	Mm.139825.
3D structure databases
ProteinModelPortal	Q8R087.
SMR	Q8R087. Positions 91-327.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8R087.
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
Proteomic databases
PRIDE	Q8R087.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000064701; ENSMUSP00000068532; ENSMUSG00000021504. ENSMUST00000100764; ENSMUSP00000098327; ENSMUSG00000021504.
GeneID	218271.
KEGG	mmu:218271.
UCSC	uc011yzw.1. mouse.
Organism-specific databases
CTD	11285.
MGI	MGI:2384987. B4galt7.
Phylogenomic databases
GeneTree	ENSGT00550000074204.
HOGENOM	HBG315803.
HOVERGEN	HBG050654.
InParanoid	Q8R087.
OMA	GWGLEDD.
OrthoDB	EOG41ZFBB.
PhylomeDB	Q8R087.
Gene expression databases
ArrayExpress	Q8R087.
Bgee	Q8R087.
Genevestigator	Q8R087.
GermOnline	ENSMUSG00000021504. Mus musculus.
Family and domain databases
InterPro	IPR003859. Galactosyl_T_2_met. [Graphical view]
KO	K00733.
PANTHER	PTHR19300. Galactosyl_T_2. 1 hit.
Pfam	PF02709. Galactosyl_T_2. 1 hit. [Graphical view]
PRINTS	PR02050. B14GALTRFASE.
ProtoNet	Search...
Other
NextBio	376224.
SOURCE	Search...

Entry information

Entry name

B4GT7_MOUSE

Accession

Primary (citable) accession number: Q8R087
Secondary accession number(s): Q7TNU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program