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Protein

Beta-1,4-galactosyltransferase 7

Gene

B4galt7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the biosynthesis of the tetrasaccharide linkage region of proteoglycans, especially for small proteoglycans in skin fibroblasts.By similarity

Catalytic activityi

UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein] = UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein].

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi165 – 1651ManganeseBy similarity
Binding sitei194 – 1941UDP-alpha-D-galactoseBy similarity
Binding sitei224 – 2241UDP-alpha-D-galactoseBy similarity
Metal bindingi257 – 2571Manganese; via tele nitrogenBy similarity
Binding sitei266 – 2661UDP-alpha-D-galactoseBy similarity

GO - Molecular functioni

  1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: MGI
  2. galactosyltransferase activity Source: UniProtKB
  3. manganese ion binding Source: UniProtKB
  4. xylosylprotein 4-beta-galactosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. extracellular fibril organization Source: UniProtKB
  2. glycosaminoglycan biosynthetic process Source: UniProtKB
  3. negative regulation of fibroblast proliferation Source: UniProtKB
  4. protein N-linked glycosylation Source: UniProtKB
  5. proteoglycan metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196489. A tetrasaccharide linker sequence is required for GAG synthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 7 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 7
Short name:
Beta4Gal-T7
Short name:
b4Gal-T7
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 7
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 7
Including the following 1 domains:
Xylosylprotein 4-beta-galactosyltransferase (EC:2.4.1.133)
Alternative name(s):
Proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I
UDP-galactose:beta-xylose beta-1,4-galactosyltransferase
XGPT
XGalT-1
Xylosylprotein beta-1,4-galactosyltransferase
Gene namesi
Name:B4galt7
Synonyms:Xgalt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:2384987. B4galt7.

Subcellular locationi

Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
Note: Cis cisternae of Golgi stack.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei31 – 5121Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini52 – 327276LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB
  2. Golgi cisterna membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Beta-1,4-galactosyltransferase 7PRO_0000080551Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8R087.
PRIDEiQ8R087.

PTM databases

PhosphoSiteiQ8R087.

Expressioni

Gene expression databases

BgeeiQ8R087.
ExpressionAtlasiQ8R087. baseline and differential.
GenevestigatoriQ8R087.

Interactioni

Protein-protein interaction databases

MINTiMINT-4997781.

Structurei

3D structure databases

ProteinModelPortaliQ8R087.
SMRiQ8R087. Positions 81-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni100 – 1045UDP-alpha-D-galactose bindingBy similarity
Regioni139 – 1413UDP-alpha-D-galactose bindingBy similarity
Regioni164 – 1652UDP-alpha-D-galactose bindingBy similarity
Regioni226 – 2294N-acetyl-D-glucosamine bindingBy similarity
Regioni257 – 2593UDP-alpha-D-galactose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG305756.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000286021.
HOVERGENiHBG050654.
InParanoidiQ8R087.
KOiK00733.
OMAiFRFNRAS.
OrthoDBiEOG7C5M8P.
PhylomeDBiQ8R087.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8R087-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLPSRRKAAQ LPWEDGRARL LPGGLRRKCS IFHLFIAFLL LVFFSLLWLQ
60 70 80 90 100
LSCSGDMAQV TRGQGQETSG PPRACPPEPP PEHWEEDESW GPHRLAVLVP
110 120 130 140 150
FRERFEELLV FVPHMHRFLS RKRIQHHIYV LNQVDHFRFN RAALINVGFL
160 170 180 190 200
ESSNSTDYIA MHDVDLLPLN EELDYGFPEA GPFHVASPEL HPLYHYKTYV
210 220 230 240 250
GGILLLSKQH YQLCNGMSNR FWGWGREDDE FYRRIKGAGL QLFRPSGITT
260 270 280 290 300
GYQTFRHLHD PAWRKRDQKR IAAQKQEQFK VDREGGLNTV KYRVDSRTAL
310 320
SIGGAPCTVL NVMLDCDKTA TPWCIFG
Length:327
Mass (Da):37,754
Last modified:June 1, 2002 - v1
Checksum:iF79B656325DCFDE0
GO
Isoform 2 (identifier: Q8R087-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-275: Missing.

Show »
Length:292
Mass (Da):33,563
Checksum:iD50E046DBEF4D599
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei241 – 27535Missing in isoform 2. 1 PublicationVSP_014231Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027195 mRNA. Translation: AAH27195.1.
BC055703 mRNA. Translation: AAH55703.1.
CCDSiCCDS26550.1. [Q8R087-1]
RefSeqiNP_666157.1. NM_146045.1. [Q8R087-1]
XP_006517279.1. XM_006517216.1. [Q8R087-2]
UniGeneiMm.139825.

Genome annotation databases

EnsembliENSMUST00000064701; ENSMUSP00000068532; ENSMUSG00000021504. [Q8R087-1]
ENSMUST00000100764; ENSMUSP00000098327; ENSMUSG00000021504. [Q8R087-2]
GeneIDi218271.
KEGGimmu:218271.
UCSCiuc007qru.1. mouse. [Q8R087-1]
uc011yzw.1. mouse. [Q8R087-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

b4GalT7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027195 mRNA. Translation: AAH27195.1.
BC055703 mRNA. Translation: AAH55703.1.
CCDSiCCDS26550.1. [Q8R087-1]
RefSeqiNP_666157.1. NM_146045.1. [Q8R087-1]
XP_006517279.1. XM_006517216.1. [Q8R087-2]
UniGeneiMm.139825.

3D structure databases

ProteinModelPortaliQ8R087.
SMRiQ8R087. Positions 81-326.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4997781.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSiteiQ8R087.

Proteomic databases

MaxQBiQ8R087.
PRIDEiQ8R087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064701; ENSMUSP00000068532; ENSMUSG00000021504. [Q8R087-1]
ENSMUST00000100764; ENSMUSP00000098327; ENSMUSG00000021504. [Q8R087-2]
GeneIDi218271.
KEGGimmu:218271.
UCSCiuc007qru.1. mouse. [Q8R087-1]
uc011yzw.1. mouse. [Q8R087-2]

Organism-specific databases

CTDi11285.
MGIiMGI:2384987. B4galt7.

Phylogenomic databases

eggNOGiNOG305756.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000286021.
HOVERGENiHBG050654.
InParanoidiQ8R087.
KOiK00733.
OMAiFRFNRAS.
OrthoDBiEOG7C5M8P.
PhylomeDBiQ8R087.
TreeFamiTF312834.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_196489. A tetrasaccharide linker sequence is required for GAG synthesis.

Miscellaneous databases

NextBioi376224.
PROiQ8R087.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R087.
ExpressionAtlasiQ8R087. baseline and differential.
GenevestigatoriQ8R087.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: FVB/N.
    Tissue: Brain and Colon.

Entry informationi

Entry nameiB4GT7_MOUSE
AccessioniPrimary (citable) accession number: Q8R087
Secondary accession number(s): Q7TNU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 1, 2002
Last modified: February 4, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.