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Protein

Heterogeneous nuclear ribonucleoprotein L

Gene

Hnrnpl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion (PubMed:22523384). Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein L
Short name:
hnRNP L
Gene namesi
Name:Hnrnpl
Synonyms:Hnrpl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:104816 Hnrnpl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Complete lethality during early embryonic development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000818631 – 586Heterogeneous nuclear ribonucleoprotein LAdd BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki59Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki62Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei98PhosphoserineBy similarity1
Cross-linki133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei182PhosphoserineBy similarity1
Modified residuei266N6-acetyllysineBy similarity1
Modified residuei288PhosphoserineBy similarity1
Modified residuei295PhosphoserineBy similarity1
Cross-linki299Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei351Asymmetric dimethylarginineCombined sources1
Modified residuei355Asymmetric dimethylarginineCombined sources1
Modified residuei378PhosphoserineCombined sources1
Modified residuei541Phosphoserine; by CaMK4By similarity1
Cross-linki565Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Phosphorylation at Ser-541 by CaMK4 enhances interaction with a CaMK4-responsive RNA element (CaRRE1), and prevents inclusion of the stress axis-regulated exon (STREX) of the KCNMA1 potassium channel transcripts upon membrane depolarization.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8R081
MaxQBiQ8R081
PaxDbiQ8R081
PeptideAtlasiQ8R081
PRIDEiQ8R081

2D gel databases

REPRODUCTION-2DPAGEiQ8R081

PTM databases

iPTMnetiQ8R081
PhosphoSitePlusiQ8R081
SwissPalmiQ8R081

Expressioni

Tissue specificityi

Detected in hematopoietic cells, including lymphoid progenitor cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000015165
CleanExiMM_HNRNPL
ExpressionAtlasiQ8R081 baseline and differential
GenevisibleiQ8R081 MM

Interactioni

Subunit structurei

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with HNRNPLL. Interacts with APEX1; the interaction is DNA-dependent. Component of a complex with SETD2 (By similarity). Interacts with ELAVL1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi200360, 8 interactors
IntActiQ8R081, 12 interactors
MINTiQ8R081
STRINGi10090.ENSMUSP00000049407

Structurei

Secondary structure

1586
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi379 – 384Combined sources6
Turni388 – 390Combined sources3
Helixi393 – 400Combined sources8
Turni401 – 403Combined sources3
Beta strandi406 – 411Combined sources6
Beta strandi419 – 425Combined sources7
Helixi426 – 436Combined sources11
Beta strandi447 – 450Combined sources4
Beta strandi452 – 455Combined sources4
Beta strandi469 – 473Combined sources5
Helixi485 – 488Combined sources4
Beta strandi498 – 505Combined sources8
Helixi511 – 521Combined sources11
Beta strandi527 – 531Combined sources5
Beta strandi536 – 545Combined sources10
Helixi549 – 559Combined sources11
Beta strandi567 – 571Combined sources5
Beta strandi576 – 578Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3S01X-ray2.15A376-586[»]
3TYTX-ray1.60A376-579[»]
ProteinModelPortaliQ8R081
SMRiQ8R081
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 173RRM 1PROSITE-ProRule annotationAdd BLAST75
Domaini190 – 267RRM 2PROSITE-ProRule annotationAdd BLAST78
Domaini379 – 476RRM 3PROSITE-ProRule annotationAdd BLAST98
Domaini492 – 580RRM 4PROSITE-ProRule annotationAdd BLAST89

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi39 – 86Gly-richAdd BLAST48
Compositional biasi332 – 379Pro-richAdd BLAST48

Domaini

RRM domain 2 has moderate RNA-binding affinity. RRM domains 3 and 4 may facilitate RNA looping when binding to two appropriately separated binding sites within the same target pre-mRNA.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1456 Eukaryota
ENOG410XQHN LUCA
GeneTreeiENSGT00550000074508
HOGENOMiHOG000293298
HOVERGENiHBG105786
InParanoidiQ8R081
KOiK13159
PhylomeDBiQ8R081

Family and domain databases

CDDicd12780 RRM1_hnRNPL, 1 hit
cd12785 RRM2_hnRNPL, 1 hit
cd12699 RRM3_hnRNPL, 1 hit
cd12704 RRM4_hnRNPL, 1 hit
Gene3Di3.30.70.330, 4 hits
InterProiView protein in InterPro
IPR006536 HnRNP-L/PTB
IPR034816 hnRNP-L_RRM3
IPR035005 hnRNPL_RRM1
IPR035008 hnRNPL_RRM2
IPR034817 hnRNPL_RRM4
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 3 hits
SUPFAMiSSF54928 SSF54928, 3 hits
TIGRFAMsiTIGR01649 hnRNP-L_PTB, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 3 hits

Sequencei

Sequence statusi: Complete.

Q8R081-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRLLPRAE KRRRRLEQRQ QPDEQLRRAG AMVKMAAAGG GGGGGRYYGG
60 70 80 90 100
GNEGGRAPKR LKTENAGDQH GGGGGGGSGA AGGGGGENYD DPHKTPASPV
110 120 130 140 150
VHIRGLIDGV VEADLVEALQ EFGPISYVVV MPKKRQALVE FEDVLGACNA
160 170 180 190 200
VNYAADNQIY IAGHPAFVNY STSQKISRPG DSDDSRSVNS VLLFTILNPI
210 220 230 240 250
YSITTDVLYT ICNPCGPVQR IVIFRKNGVQ AMVEFDSVQS AQRAKASLNG
260 270 280 290 300
ADIYSGCCTL KIEYAKPTRL NVFKNDQDTW DYTNPNLSGQ GDPGSNPNKR
310 320 330 340 350
QRQPPLLGDH PAEYGGPHGG YHSHYHDEGY GPPPPHYEGR RMGPPVGGHR
360 370 380 390 400
RGPSRYGPQY GHPPPPPPPP DYGPHADSPV LMVYGLDQSK MNCDRVFNVF
410 420 430 440 450
CLYGNVEKVK FMKSKPGAAM VEMADGYAVD RAITHLNNNF MFGQKMNVCV
460 470 480 490 500
SKQPAIMPGQ SYGLEDGSCS YKDFSESRNN RFSTPEQAAK NRIQHPSNVL
510 520 530 540 550
HFFNAPLEVT EENFFEICDE LGVKRPTSVK VFSGKSERSS SGLLEWDSKS
560 570 580
DALETLGFLN HYQMKNPNGP YPYTLKLCFS TAQHAS
Length:586
Mass (Da):63,964
Last modified:November 25, 2008 - v2
Checksum:iBB56D3D6A8553F7E
GO

Sequence cautioni

The sequence AAH27206 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti388Q → E in BAA24237 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027206 mRNA Translation: AAH27206.1 Different initiation.
BC030461 mRNA Translation: AAH30461.1
BC099683 mRNA Translation: AAH99683.1
AB009392 mRNA Translation: BAA24237.1
CCDSiCCDS39864.2
RefSeqiNP_796275.3, NM_177301.5
XP_006539621.1, XM_006539558.2
UniGeneiMm.9043

Genome annotation databases

EnsembliENSMUST00000038572; ENSMUSP00000049407; ENSMUSG00000015165
ENSMUST00000174548; ENSMUSP00000133728; ENSMUSG00000015165
GeneIDi15388
KEGGimmu:15388
UCSCiuc009fzz.2 mouse

Similar proteinsi

Entry informationi

Entry nameiHNRPL_MOUSE
AccessioniPrimary (citable) accession number: Q8R081
Secondary accession number(s): O54789, Q499X2, Q8K0S7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: November 25, 2008
Last modified: April 25, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health