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Protein

Heterogeneous nuclear ribonucleoprotein L

Gene

Hnrnpl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion (PubMed:22523384). Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • mRNA processing Source: InterPro
  • regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72203. Processing of Capped Intron-Containing Pre-mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein L
Short name:
hnRNP L
Gene namesi
Name:Hnrnpl
Synonyms:Hnrpl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:104816. Hnrnpl.

Subcellular locationi

  • Nucleusnucleoplasm By similarity
  • Cytoplasm By similarity

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. These granules are not identical with P bodies or stress granules.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Complete lethality during early embryonic development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000818631 – 586Heterogeneous nuclear ribonucleoprotein LAdd BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki62Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei98PhosphoserineBy similarity1
Cross-linki133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei182PhosphoserineBy similarity1
Modified residuei266N6-acetyllysineBy similarity1
Modified residuei288PhosphoserineBy similarity1
Modified residuei295PhosphoserineBy similarity1
Modified residuei351Asymmetric dimethylarginineCombined sources1
Modified residuei355Asymmetric dimethylarginineCombined sources1
Modified residuei378PhosphoserineCombined sources1
Modified residuei541Phosphoserine; by CaMK4By similarity1

Post-translational modificationi

Phosphorylation at Ser-541 by CaMK4 enhances interaction with a CaMK4-responsive RNA element (CaRRE1), and prevents inclusion of the stress axis-regulated exon (STREX) of the KCNMA1 potassium channel transcripts upon membrane depolarization.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8R081.
MaxQBiQ8R081.
PaxDbiQ8R081.
PeptideAtlasiQ8R081.
PRIDEiQ8R081.

2D gel databases

REPRODUCTION-2DPAGEQ8R081.

PTM databases

iPTMnetiQ8R081.
PhosphoSitePlusiQ8R081.
SwissPalmiQ8R081.

Expressioni

Tissue specificityi

Detected in hematopoietic cells, including lymphoid progenitor cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000015165.
CleanExiMM_HNRNPL.
ExpressionAtlasiQ8R081. baseline and differential.
GenevisibleiQ8R081. MM.

Interactioni

Subunit structurei

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with HNRNPLL. Interacts with APEX1; the interaction is DNA-dependent. Component of a complex with SETD2 (By similarity). Interacts with ELAVL1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi200360. 6 interactors.
IntActiQ8R081. 12 interactors.
MINTiMINT-4097731.
STRINGi10090.ENSMUSP00000049407.

Structurei

Secondary structure

1586
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi379 – 384Combined sources6
Turni388 – 390Combined sources3
Helixi393 – 400Combined sources8
Turni401 – 403Combined sources3
Beta strandi406 – 411Combined sources6
Beta strandi419 – 425Combined sources7
Helixi426 – 436Combined sources11
Beta strandi447 – 450Combined sources4
Beta strandi452 – 455Combined sources4
Beta strandi469 – 473Combined sources5
Helixi485 – 488Combined sources4
Beta strandi498 – 505Combined sources8
Helixi511 – 521Combined sources11
Beta strandi527 – 531Combined sources5
Beta strandi536 – 545Combined sources10
Helixi549 – 559Combined sources11
Beta strandi567 – 571Combined sources5
Beta strandi576 – 578Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3S01X-ray2.15A376-586[»]
3TYTX-ray1.60A376-579[»]
ProteinModelPortaliQ8R081.
SMRiQ8R081.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 173RRM 1PROSITE-ProRule annotationAdd BLAST75
Domaini190 – 267RRM 2PROSITE-ProRule annotationAdd BLAST78
Domaini379 – 476RRM 3PROSITE-ProRule annotationAdd BLAST98
Domaini492 – 580RRM 4PROSITE-ProRule annotationAdd BLAST89

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi39 – 86Gly-richAdd BLAST48
Compositional biasi332 – 379Pro-richAdd BLAST48

Domaini

RRM domain 2 has moderate RNA-binding affinity. RRM domains 3 and 4 may facilitate RNA looping when binding to two appropriately separated binding sites within the same target pre-mRNA.By similarity

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1456. Eukaryota.
ENOG410XQHN. LUCA.
GeneTreeiENSGT00550000074508.
HOGENOMiHOG000293298.
HOVERGENiHBG105786.
InParanoidiQ8R081.
KOiK13159.
PhylomeDBiQ8R081.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R081-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRLLPRAE KRRRRLEQRQ QPDEQLRRAG AMVKMAAAGG GGGGGRYYGG
60 70 80 90 100
GNEGGRAPKR LKTENAGDQH GGGGGGGSGA AGGGGGENYD DPHKTPASPV
110 120 130 140 150
VHIRGLIDGV VEADLVEALQ EFGPISYVVV MPKKRQALVE FEDVLGACNA
160 170 180 190 200
VNYAADNQIY IAGHPAFVNY STSQKISRPG DSDDSRSVNS VLLFTILNPI
210 220 230 240 250
YSITTDVLYT ICNPCGPVQR IVIFRKNGVQ AMVEFDSVQS AQRAKASLNG
260 270 280 290 300
ADIYSGCCTL KIEYAKPTRL NVFKNDQDTW DYTNPNLSGQ GDPGSNPNKR
310 320 330 340 350
QRQPPLLGDH PAEYGGPHGG YHSHYHDEGY GPPPPHYEGR RMGPPVGGHR
360 370 380 390 400
RGPSRYGPQY GHPPPPPPPP DYGPHADSPV LMVYGLDQSK MNCDRVFNVF
410 420 430 440 450
CLYGNVEKVK FMKSKPGAAM VEMADGYAVD RAITHLNNNF MFGQKMNVCV
460 470 480 490 500
SKQPAIMPGQ SYGLEDGSCS YKDFSESRNN RFSTPEQAAK NRIQHPSNVL
510 520 530 540 550
HFFNAPLEVT EENFFEICDE LGVKRPTSVK VFSGKSERSS SGLLEWDSKS
560 570 580
DALETLGFLN HYQMKNPNGP YPYTLKLCFS TAQHAS
Length:586
Mass (Da):63,964
Last modified:November 25, 2008 - v2
Checksum:iBB56D3D6A8553F7E
GO

Sequence cautioni

The sequence AAH27206 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti388Q → E in BAA24237 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027206 mRNA. Translation: AAH27206.1. Different initiation.
BC030461 mRNA. Translation: AAH30461.1.
BC099683 mRNA. Translation: AAH99683.1.
AB009392 mRNA. Translation: BAA24237.1.
CCDSiCCDS39864.2.
RefSeqiNP_796275.3. NM_177301.5.
XP_006539621.1. XM_006539558.2.
UniGeneiMm.9043.

Genome annotation databases

EnsembliENSMUST00000038572; ENSMUSP00000049407; ENSMUSG00000015165.
ENSMUST00000174548; ENSMUSP00000133728; ENSMUSG00000015165.
GeneIDi15388.
KEGGimmu:15388.
UCSCiuc009fzz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027206 mRNA. Translation: AAH27206.1. Different initiation.
BC030461 mRNA. Translation: AAH30461.1.
BC099683 mRNA. Translation: AAH99683.1.
AB009392 mRNA. Translation: BAA24237.1.
CCDSiCCDS39864.2.
RefSeqiNP_796275.3. NM_177301.5.
XP_006539621.1. XM_006539558.2.
UniGeneiMm.9043.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3S01X-ray2.15A376-586[»]
3TYTX-ray1.60A376-579[»]
ProteinModelPortaliQ8R081.
SMRiQ8R081.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200360. 6 interactors.
IntActiQ8R081. 12 interactors.
MINTiMINT-4097731.
STRINGi10090.ENSMUSP00000049407.

PTM databases

iPTMnetiQ8R081.
PhosphoSitePlusiQ8R081.
SwissPalmiQ8R081.

2D gel databases

REPRODUCTION-2DPAGEQ8R081.

Proteomic databases

EPDiQ8R081.
MaxQBiQ8R081.
PaxDbiQ8R081.
PeptideAtlasiQ8R081.
PRIDEiQ8R081.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038572; ENSMUSP00000049407; ENSMUSG00000015165.
ENSMUST00000174548; ENSMUSP00000133728; ENSMUSG00000015165.
GeneIDi15388.
KEGGimmu:15388.
UCSCiuc009fzz.2. mouse.

Organism-specific databases

CTDi3191.
MGIiMGI:104816. Hnrnpl.

Phylogenomic databases

eggNOGiKOG1456. Eukaryota.
ENOG410XQHN. LUCA.
GeneTreeiENSGT00550000074508.
HOGENOMiHOG000293298.
HOVERGENiHBG105786.
InParanoidiQ8R081.
KOiK13159.
PhylomeDBiQ8R081.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72203. Processing of Capped Intron-Containing Pre-mRNA.

Miscellaneous databases

ChiTaRSiHnrnpl. mouse.
PROiQ8R081.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015165.
CleanExiMM_HNRNPL.
ExpressionAtlasiQ8R081. baseline and differential.
GenevisibleiQ8R081. MM.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHNRPL_MOUSE
AccessioniPrimary (citable) accession number: Q8R081
Secondary accession number(s): O54789, Q499X2, Q8K0S7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: November 25, 2008
Last modified: November 30, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.