Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8R081

- HNRPL_MOUSE

UniProt

Q8R081 - HNRPL_MOUSE

Protein

Heterogeneous nuclear ribonucleoprotein L

Gene

Hnrnpl

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (25 Nov 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion. Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. RNA binding Source: UniProtKB-KW
    3. transcription regulatory region DNA binding Source: UniProtKB

    GO - Biological processi

    1. mRNA processing Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heterogeneous nuclear ribonucleoprotein L
    Short name:
    hnRNP L
    Gene namesi
    Name:Hnrnpl
    Synonyms:Hnrpl
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:104816. Hnrnpl.

    Subcellular locationi

    Nucleusnucleoplasm By similarity. Cytoplasm By similarity
    Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleoplasm Source: UniProtKB-SubCell
    3. nucleus Source: MGI
    4. pronucleus Source: MGI
    5. ribonucleoprotein complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 586586Heterogeneous nuclear ribonucleoprotein LPRO_0000081863Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei98 – 981PhosphoserineBy similarity
    Modified residuei182 – 1821PhosphoserineBy similarity
    Modified residuei266 – 2661N6-acetyllysineBy similarity
    Modified residuei288 – 2881PhosphoserineBy similarity
    Modified residuei295 – 2951PhosphoserineBy similarity
    Modified residuei541 – 5411Phosphoserine; by CaMK4By similarity

    Post-translational modificationi

    Phosphorylation at Ser-541 by CaMK4 enhances interaction with a CaMK4-responsive RNA element (CaRRE1), and prevents inclusion of the stress axis-regulated exon (STREX) of the KCNMA1 potassium channel transcripts upon membrane depolarization.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8R081.
    PaxDbiQ8R081.
    PRIDEiQ8R081.

    2D gel databases

    REPRODUCTION-2DPAGEQ8R081.

    PTM databases

    PhosphoSiteiQ8R081.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8R081.
    BgeeiQ8R081.
    CleanExiMM_HNRNPL.
    GenevestigatoriQ8R081.

    Interactioni

    Subunit structurei

    Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with HNRNPLL. Interacts with APEX1; the interaction is DNA-dependent. Component of a complex with SETD2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi200360. 4 interactions.
    IntActiQ8R081. 6 interactions.
    MINTiMINT-4097731.

    Structurei

    Secondary structure

    1
    586
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi379 – 3846
    Turni388 – 3903
    Helixi393 – 4008
    Turni401 – 4033
    Beta strandi406 – 4116
    Beta strandi419 – 4257
    Helixi426 – 43611
    Beta strandi447 – 4504
    Beta strandi452 – 4554
    Beta strandi469 – 4735
    Helixi485 – 4884
    Beta strandi498 – 5058
    Helixi511 – 52111
    Beta strandi527 – 5315
    Beta strandi536 – 54510
    Helixi549 – 55911
    Beta strandi567 – 5715
    Beta strandi576 – 5783

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3S01X-ray2.15A376-586[»]
    3TYTX-ray1.60A376-579[»]
    ProteinModelPortaliQ8R081.
    SMRiQ8R081. Positions 94-287, 376-579.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini99 – 17375RRM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini190 – 26778RRM 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini379 – 47698RRM 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini492 – 58089RRM 4PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi39 – 8648Gly-richAdd
    BLAST
    Compositional biasi332 – 37948Pro-richAdd
    BLAST

    Domaini

    RRM domain 2 has moderate RNA-binding affinity. RRM domains 3 and 4 may facilitate RNA looping when binding to two appropriately separated binding sites within the same target pre-mRNA By similarity.By similarity

    Sequence similaritiesi

    Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG326285.
    GeneTreeiENSGT00550000074508.
    HOGENOMiHOG000293298.
    HOVERGENiHBG105786.
    KOiK13159.
    OrthoDBiEOG7HMS2K.
    PhylomeDBiQ8R081.

    Family and domain databases

    Gene3Di3.30.70.330. 4 hits.
    InterProiIPR006536. HnRNP-L_PTB.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    SMARTiSM00360. RRM. 3 hits.
    [Graphical view]
    TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
    PROSITEiPS50102. RRM. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8R081-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRRLLPRAE KRRRRLEQRQ QPDEQLRRAG AMVKMAAAGG GGGGGRYYGG    50
    GNEGGRAPKR LKTENAGDQH GGGGGGGSGA AGGGGGENYD DPHKTPASPV 100
    VHIRGLIDGV VEADLVEALQ EFGPISYVVV MPKKRQALVE FEDVLGACNA 150
    VNYAADNQIY IAGHPAFVNY STSQKISRPG DSDDSRSVNS VLLFTILNPI 200
    YSITTDVLYT ICNPCGPVQR IVIFRKNGVQ AMVEFDSVQS AQRAKASLNG 250
    ADIYSGCCTL KIEYAKPTRL NVFKNDQDTW DYTNPNLSGQ GDPGSNPNKR 300
    QRQPPLLGDH PAEYGGPHGG YHSHYHDEGY GPPPPHYEGR RMGPPVGGHR 350
    RGPSRYGPQY GHPPPPPPPP DYGPHADSPV LMVYGLDQSK MNCDRVFNVF 400
    CLYGNVEKVK FMKSKPGAAM VEMADGYAVD RAITHLNNNF MFGQKMNVCV 450
    SKQPAIMPGQ SYGLEDGSCS YKDFSESRNN RFSTPEQAAK NRIQHPSNVL 500
    HFFNAPLEVT EENFFEICDE LGVKRPTSVK VFSGKSERSS SGLLEWDSKS 550
    DALETLGFLN HYQMKNPNGP YPYTLKLCFS TAQHAS 586
    Length:586
    Mass (Da):63,964
    Last modified:November 25, 2008 - v2
    Checksum:iBB56D3D6A8553F7E
    GO

    Sequence cautioni

    The sequence AAH27206.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti388 – 3881Q → E in BAA24237. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC027206 mRNA. Translation: AAH27206.1. Different initiation.
    BC030461 mRNA. Translation: AAH30461.1.
    BC099683 mRNA. Translation: AAH99683.1.
    AB009392 mRNA. Translation: BAA24237.1.
    CCDSiCCDS39864.2.
    RefSeqiNP_796275.3. NM_177301.5.
    XP_006539621.1. XM_006539558.1.
    UniGeneiMm.9043.

    Genome annotation databases

    EnsembliENSMUST00000038572; ENSMUSP00000049407; ENSMUSG00000015165.
    ENSMUST00000174548; ENSMUSP00000133728; ENSMUSG00000015165.
    GeneIDi15388.
    KEGGimmu:15388.
    UCSCiuc009fzz.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC027206 mRNA. Translation: AAH27206.1 . Different initiation.
    BC030461 mRNA. Translation: AAH30461.1 .
    BC099683 mRNA. Translation: AAH99683.1 .
    AB009392 mRNA. Translation: BAA24237.1 .
    CCDSi CCDS39864.2.
    RefSeqi NP_796275.3. NM_177301.5.
    XP_006539621.1. XM_006539558.1.
    UniGenei Mm.9043.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3S01 X-ray 2.15 A 376-586 [» ]
    3TYT X-ray 1.60 A 376-579 [» ]
    ProteinModelPortali Q8R081.
    SMRi Q8R081. Positions 94-287, 376-579.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200360. 4 interactions.
    IntActi Q8R081. 6 interactions.
    MINTi MINT-4097731.

    PTM databases

    PhosphoSitei Q8R081.

    2D gel databases

    REPRODUCTION-2DPAGE Q8R081.

    Proteomic databases

    MaxQBi Q8R081.
    PaxDbi Q8R081.
    PRIDEi Q8R081.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000038572 ; ENSMUSP00000049407 ; ENSMUSG00000015165 .
    ENSMUST00000174548 ; ENSMUSP00000133728 ; ENSMUSG00000015165 .
    GeneIDi 15388.
    KEGGi mmu:15388.
    UCSCi uc009fzz.2. mouse.

    Organism-specific databases

    CTDi 3191.
    MGIi MGI:104816. Hnrnpl.

    Phylogenomic databases

    eggNOGi NOG326285.
    GeneTreei ENSGT00550000074508.
    HOGENOMi HOG000293298.
    HOVERGENi HBG105786.
    KOi K13159.
    OrthoDBi EOG7HMS2K.
    PhylomeDBi Q8R081.

    Miscellaneous databases

    ChiTaRSi HNRNPL. mouse.
    NextBioi 288066.
    PROi Q8R081.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8R081.
    Bgeei Q8R081.
    CleanExi MM_HNRNPL.
    Genevestigatori Q8R081.

    Family and domain databases

    Gene3Di 3.30.70.330. 4 hits.
    InterProi IPR006536. HnRNP-L_PTB.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    SMARTi SM00360. RRM. 3 hits.
    [Graphical view ]
    TIGRFAMsi TIGR01649. hnRNP-L_PTB. 1 hit.
    PROSITEi PS50102. RRM. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon and Salivary gland.
    2. "Mouse ribonucleoprotein."
      Sakai N., Saitou Y., Toyota T.
      Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 388-586.
    3. Lubec G., Yang J.W., Zigmond M.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 396-408 AND 566-576.
      Tissue: Brain.
    4. "Crystal structure of a heterogeneous nuclear ribonucleoprotein l (hnrpl) from Mus musculus at 1.60 a resolution."
      Joint center for structural genomics (JCSG)
      Submitted (NOV-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 376-579.

    Entry informationi

    Entry nameiHNRPL_MOUSE
    AccessioniPrimary (citable) accession number: Q8R081
    Secondary accession number(s): O54789, Q499X2, Q8K0S7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2004
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3