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Protein

UDP-glucose 4-epimerase

Gene

Gale

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.By similarity

Catalytic activityi

UDP-alpha-D-glucose = UDP-alpha-D-galactose.By similarity
UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-galactosamine.By similarity

Cofactori

NAD+By similarity

Pathwayi: galactose metabolism

This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87NAD; via carbonyl oxygenBy similarity1
Binding sitei91NADBy similarity1
Active sitei156Proton acceptorBy similarity1
Binding sitei160NADBy similarity1
Binding sitei184NADBy similarity1
Binding sitei238SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 13NADBy similarity3
Nucleotide bindingi32 – 36NADBy similarity5
Nucleotide bindingi65 – 66NADBy similarity2

GO - Molecular functioni

GO - Biological processi

  • galactose catabolic process Source: MGI
  • galactose catabolic process via UDP-galactose Source: MGI
  • galactose metabolic process Source: MGI
  • glycolytic process from galactose Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-MMU-70370. Galactose catabolism.
UniPathwayiUPA00214.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose 4-epimeraseBy similarity (EC:5.1.3.2By similarity)
Alternative name(s):
GalactowaldenaseBy similarity
UDP-N-acetylglucosamine 4-epimeraseBy similarity (EC:5.1.3.7By similarity)
Short name:
UDP-GlcNAc 4-epimeraseBy similarity
UDP-galactosamine 4-epimeraseBy similarity
Short name:
UDP-GalNAc 4-epimeraseBy similarity
UDP-galactose 4-epimeraseBy similarity
Gene namesi
Name:Gale
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1921496. Gale.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001831901 – 347UDP-glucose 4-epimeraseAdd BLAST347

Proteomic databases

EPDiQ8R059.
MaxQBiQ8R059.
PaxDbiQ8R059.
PeptideAtlasiQ8R059.
PRIDEiQ8R059.

PTM databases

iPTMnetiQ8R059.
PhosphoSitePlusiQ8R059.
SwissPalmiQ8R059.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028671.
CleanExiMM_GALE.
ExpressionAtlasiQ8R059. baseline and differential.
GenevisibleiQ8R059. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8R059. 2 interactors.
MINTiMINT-4095506.
STRINGi10090.ENSMUSP00000099599.

Structurei

3D structure databases

ProteinModelPortaliQ8R059.
SMRiQ8R059.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 133Substrate bindingBy similarity3
Regioni184 – 186Substrate bindingBy similarity3
Regioni205 – 207Substrate bindingBy similarity3
Regioni223 – 225Substrate bindingBy similarity3
Regioni299 – 302Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1371. Eukaryota.
COG1087. LUCA.
GeneTreeiENSGT00530000063128.
HOGENOMiHOG000168001.
HOVERGENiHBG001396.
InParanoidiQ8R059.
KOiK01784.
OMAiCGCKVYN.
OrthoDBiEOG091G0ACI.
PhylomeDBiQ8R059.
TreeFamiTF105800.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR005886. GalE.
IPR016040. NAD(P)-bd_dom.
IPR008089. Nuc_sugar_epim.
[Graphical view]
PfamiPF16363. GDP_Man_Dehyd. 1 hit.
[Graphical view]
PRINTSiPR01713. NUCEPIMERASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01179. galE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8R059-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKVLVTGGA GYIGSHTVLE LLEAGYSPVV IDNFHNAIRG EDSMPESLRR
60 70 80 90 100
VQELTGRSVE FEEMDILDQA ALQHLFKKHS FKAVIHFAGL KAVGESVQKP
110 120 130 140 150
LDYYRVNLTG TIQLLEIMRA HGVKNLVFSS SATVYGNPQY LPLDEAHPTG
160 170 180 190 200
GCTNPYGKSK FFIEEMIRDL CRADTAWNAV LLRYFNPIGA HASGRIGEDP
210 220 230 240 250
QGIPNNLMPY VSQVAIGRRE ALNVFGDDYA TEDGTGVRDY IHVVDLAKGH
260 270 280 290 300
IAALKKLKEQ CGCRTYNLGT GTGYSVLQMV QAMEKASGKK IPYKVVARRE
310 320 330 340
GDVAACYANP SLAHEELGWT AALGLDRMCE DLWRWQKQNP SGFGAQA
Length:347
Mass (Da):38,225
Last modified:June 1, 2002 - v1
Checksum:i8EAE03B38040CED5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027438 mRNA. Translation: AAH27438.1.
CCDSiCCDS18796.1.
RefSeqiNP_848476.1. NM_178389.3.
XP_006539280.1. XM_006539217.1.
XP_006539281.1. XM_006539218.3.
XP_006539282.1. XM_006539219.3.
UniGeneiMm.247946.

Genome annotation databases

EnsembliENSMUST00000102540; ENSMUSP00000099599; ENSMUSG00000028671.
ENSMUST00000102541; ENSMUSP00000099600; ENSMUSG00000028671.
GeneIDi74246.
KEGGimmu:74246.
UCSCiuc008vhk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027438 mRNA. Translation: AAH27438.1.
CCDSiCCDS18796.1.
RefSeqiNP_848476.1. NM_178389.3.
XP_006539280.1. XM_006539217.1.
XP_006539281.1. XM_006539218.3.
XP_006539282.1. XM_006539219.3.
UniGeneiMm.247946.

3D structure databases

ProteinModelPortaliQ8R059.
SMRiQ8R059.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R059. 2 interactors.
MINTiMINT-4095506.
STRINGi10090.ENSMUSP00000099599.

PTM databases

iPTMnetiQ8R059.
PhosphoSitePlusiQ8R059.
SwissPalmiQ8R059.

Proteomic databases

EPDiQ8R059.
MaxQBiQ8R059.
PaxDbiQ8R059.
PeptideAtlasiQ8R059.
PRIDEiQ8R059.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102540; ENSMUSP00000099599; ENSMUSG00000028671.
ENSMUST00000102541; ENSMUSP00000099600; ENSMUSG00000028671.
GeneIDi74246.
KEGGimmu:74246.
UCSCiuc008vhk.1. mouse.

Organism-specific databases

CTDi2582.
MGIiMGI:1921496. Gale.

Phylogenomic databases

eggNOGiKOG1371. Eukaryota.
COG1087. LUCA.
GeneTreeiENSGT00530000063128.
HOGENOMiHOG000168001.
HOVERGENiHBG001396.
InParanoidiQ8R059.
KOiK01784.
OMAiCGCKVYN.
OrthoDBiEOG091G0ACI.
PhylomeDBiQ8R059.
TreeFamiTF105800.

Enzyme and pathway databases

UniPathwayiUPA00214.
ReactomeiR-MMU-70370. Galactose catabolism.

Miscellaneous databases

ChiTaRSiGale. mouse.
PROiQ8R059.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028671.
CleanExiMM_GALE.
ExpressionAtlasiQ8R059. baseline and differential.
GenevisibleiQ8R059. MM.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR005886. GalE.
IPR016040. NAD(P)-bd_dom.
IPR008089. Nuc_sugar_epim.
[Graphical view]
PfamiPF16363. GDP_Man_Dehyd. 1 hit.
[Graphical view]
PRINTSiPR01713. NUCEPIMERASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01179. galE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGALE_MOUSE
AccessioniPrimary (citable) accession number: Q8R059
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.