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Protein

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A

Gene

Gspt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi218 – 225GTPBy similarity8
Nucleotide bindingi295 – 299GTPBy similarity5
Nucleotide bindingi357 – 360GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Nonsense-mediated mRNA decay, Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-72764. Eukaryotic Translation Termination.
R-MMU-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Short name:
Eukaryotic peptide chain release factor subunit 3a
Short name:
eRF3a
Alternative name(s):
G1 to S phase transition protein 1 homolog
Gene namesi
Name:Gspt1
Synonyms:Erf3a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1316728. Gspt1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000914811 – 636Eukaryotic peptide chain release factor GTP-binding subunit ERF3AAdd BLAST636

Proteomic databases

PaxDbiQ8R050.
PeptideAtlasiQ8R050.
PRIDEiQ8R050.

PTM databases

iPTMnetiQ8R050.
PhosphoSitePlusiQ8R050.
SwissPalmiQ8R050.

Expressioni

Gene expression databases

BgeeiENSMUSG00000062203.
CleanExiMM_GSPT1.
ExpressionAtlasiQ8R050. baseline and differential.
GenevisibleiQ8R050. MM.

Interactioni

Subunit structurei

Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex (By similarity). Interacts with PABPC1.By similarity1 Publication

Protein-protein interaction databases

BioGridi200087. 38 interactors.
IntActiQ8R050. 38 interactors.
MINTiMINT-4117272.
STRINGi10090.ENSMUSP00000078940.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RQGNMR-A64-82[»]
2RQHNMR-A73-94[»]
ProteinModelPortaliQ8R050.
SMRiQ8R050.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini209 – 435tr-type GPROSITE-ProRule annotationAdd BLAST227

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 225G1PROSITE-ProRule annotation8
Regioni274 – 278G2PROSITE-ProRule annotation5
Regioni295 – 298G3PROSITE-ProRule annotation4
Regioni357 – 360G4PROSITE-ProRule annotation4
Regioni399 – 401G5PROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0459. Eukaryota.
COG5256. LUCA.
GeneTreeiENSGT00620000087924.
HOGENOMiHOG000229291.
HOVERGENiHBG000179.
InParanoidiQ8R050.
KOiK03267.
OMAiLCPWYRG.
OrthoDBiEOG091G0637.
TreeFamiTF300566.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR009818. Ataxin-2_C.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
PF07145. PAM2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R050-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPSSGGGGG GGGGGSSSSS DSAPDCWDQT DMEAPGPGPC GGGGSGSGSM
60 70 80 90 100
AAVAEAQREN LSAAFSRQLN VNAKPFVPNV HAAEFVPSFL RGPAQPPLSP
110 120 130 140 150
AGAAGGDHGA GSGAGGPSEP VESSQDQSCE GSNSTVSMEL SEPVVENGET
160 170 180 190 200
EMSPEESWEH KEEISEAEPG GGSSGDGRPP EESTQEMMEE EEEIPKPKSA
210 220 230 240 250
VAPPGAPKKE HVNVVFIGHV DAGKSTIGGQ IMYLTGMVDK RTLEKYEREA
260 270 280 290 300
KEKNRETWYL SWALDTNQEE RDKGKTVEVG RAYFETEKKH FTILDAPGHK
310 320 330 340 350
SFVPNMIGGA SQADLAVLVI SARKGEFETG FEKGGQTREH AMLAKTAGVK
360 370 380 390 400
HLIVLINKMD DPTVNWSNER YEECKEKLVP FLKKVGFNPK KDIHFMPCSG
410 420 430 440 450
LTGANLKEQS DFCPWYIGLP FIPYLDNLPN FNRSVDGPIR LPIVDKYKDM
460 470 480 490 500
GTVVLGKLES GSICKGQQLV MMPNKHNVEV LGILSDDVET DSVAPGENLK
510 520 530 540 550
IRLKGIEEEE ILPGFILCDL NNLCHSGRTF DAQIVIIEHK SIICPGYNAV
560 570 580 590 600
LHIHTCIEEV EITALICLVD KKSGEKSKTR PRFVKQDQVC IARLRTAGTI
610 620 630
CLETFKDFPQ MGRFTLRDEG KTIAIGKVLK LVPEKD
Length:636
Mass (Da):68,625
Last modified:June 13, 2012 - v2
Checksum:i28EC54B408D3F5A3
GO
Isoform 2 (identifier: Q8R050-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     145-145: Missing.

Show »
Length:635
Mass (Da):68,526
Checksum:i3EF70378C701C6BC
GO

Sequence cautioni

The sequence AAH28325 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH31640 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41G → A in AAH31640 (PubMed:15489334).Curated1
Sequence conflicti485S → C in BAA32526 (PubMed:9712840).Curated1
Sequence conflicti615T → R in BAA32526 (PubMed:9712840).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_043829145Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC087541 Genomic DNA. No translation available.
CH466521 Genomic DNA. Translation: EDK97345.1.
BC028325 mRNA. Translation: AAH28325.1. Different initiation.
BC031640 mRNA. Translation: AAH31640.1. Different initiation.
AB003502 mRNA. Translation: BAA32526.1.
CCDSiCCDS27961.2. [Q8R050-1]
CCDS49764.1. [Q8R050-2]
RefSeqiNP_001123480.1. NM_001130008.1. [Q8R050-2]
NP_666178.2. NM_146066.2. [Q8R050-1]
UniGeneiMm.325827.

Genome annotation databases

EnsembliENSMUST00000080030; ENSMUSP00000078940; ENSMUSG00000062203. [Q8R050-1]
ENSMUST00000167571; ENSMUSP00000130583; ENSMUSG00000062203. [Q8R050-2]
GeneIDi14852.
KEGGimmu:14852.
UCSCiuc007yfe.2. mouse. [Q8R050-1]
uc007yff.2. mouse. [Q8R050-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC087541 Genomic DNA. No translation available.
CH466521 Genomic DNA. Translation: EDK97345.1.
BC028325 mRNA. Translation: AAH28325.1. Different initiation.
BC031640 mRNA. Translation: AAH31640.1. Different initiation.
AB003502 mRNA. Translation: BAA32526.1.
CCDSiCCDS27961.2. [Q8R050-1]
CCDS49764.1. [Q8R050-2]
RefSeqiNP_001123480.1. NM_001130008.1. [Q8R050-2]
NP_666178.2. NM_146066.2. [Q8R050-1]
UniGeneiMm.325827.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RQGNMR-A64-82[»]
2RQHNMR-A73-94[»]
ProteinModelPortaliQ8R050.
SMRiQ8R050.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200087. 38 interactors.
IntActiQ8R050. 38 interactors.
MINTiMINT-4117272.
STRINGi10090.ENSMUSP00000078940.

PTM databases

iPTMnetiQ8R050.
PhosphoSitePlusiQ8R050.
SwissPalmiQ8R050.

Proteomic databases

PaxDbiQ8R050.
PeptideAtlasiQ8R050.
PRIDEiQ8R050.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080030; ENSMUSP00000078940; ENSMUSG00000062203. [Q8R050-1]
ENSMUST00000167571; ENSMUSP00000130583; ENSMUSG00000062203. [Q8R050-2]
GeneIDi14852.
KEGGimmu:14852.
UCSCiuc007yfe.2. mouse. [Q8R050-1]
uc007yff.2. mouse. [Q8R050-2]

Organism-specific databases

CTDi2935.
MGIiMGI:1316728. Gspt1.

Phylogenomic databases

eggNOGiKOG0459. Eukaryota.
COG5256. LUCA.
GeneTreeiENSGT00620000087924.
HOGENOMiHOG000229291.
HOVERGENiHBG000179.
InParanoidiQ8R050.
KOiK03267.
OMAiLCPWYRG.
OrthoDBiEOG091G0637.
TreeFamiTF300566.

Enzyme and pathway databases

ReactomeiR-MMU-72764. Eukaryotic Translation Termination.
R-MMU-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

ChiTaRSiGspt1. mouse.
PROiQ8R050.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000062203.
CleanExiMM_GSPT1.
ExpressionAtlasiQ8R050. baseline and differential.
GenevisibleiQ8R050. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR009818. Ataxin-2_C.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
PF07145. PAM2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERF3A_MOUSE
AccessioniPrimary (citable) accession number: Q8R050
Secondary accession number(s): E9QK49
, G3UWC0, O88179, Q8K2E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: June 13, 2012
Last modified: November 2, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.