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Protein

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A

Gene

Gspt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi218 – 225GTPBy similarity8
Nucleotide bindingi295 – 299GTPBy similarity5
Nucleotide bindingi357 – 360GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processNonsense-mediated mRNA decay, Protein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-72764 Eukaryotic Translation Termination
R-MMU-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Short name:
Eukaryotic peptide chain release factor subunit 3a
Short name:
eRF3a
Alternative name(s):
G1 to S phase transition protein 1 homolog
Gene namesi
Name:Gspt1
Synonyms:Erf3a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1316728 Gspt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000914811 – 636Eukaryotic peptide chain release factor GTP-binding subunit ERF3AAdd BLAST636

Proteomic databases

PaxDbiQ8R050
PeptideAtlasiQ8R050
PRIDEiQ8R050

PTM databases

iPTMnetiQ8R050
PhosphoSitePlusiQ8R050
SwissPalmiQ8R050

Expressioni

Gene expression databases

BgeeiENSMUSG00000062203
CleanExiMM_GSPT1
ExpressionAtlasiQ8R050 baseline and differential
GenevisibleiQ8R050 MM

Interactioni

Subunit structurei

Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex (By similarity). Interacts with PABPC1.By similarity1 Publication

Protein-protein interaction databases

BioGridi200087, 38 interactors
IntActiQ8R050, 38 interactors
MINTiQ8R050
STRINGi10090.ENSMUSP00000078940

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RQGNMR-A64-82[»]
2RQHNMR-A73-94[»]
ProteinModelPortaliQ8R050
SMRiQ8R050
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini209 – 435tr-type GPROSITE-ProRule annotationAdd BLAST227

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 225G1PROSITE-ProRule annotation8
Regioni274 – 278G2PROSITE-ProRule annotation5
Regioni295 – 298G3PROSITE-ProRule annotation4
Regioni357 – 360G4PROSITE-ProRule annotation4
Regioni399 – 401G5PROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0459 Eukaryota
COG5256 LUCA
GeneTreeiENSGT00620000087924
HOGENOMiHOG000229291
HOVERGENiHBG000179
InParanoidiQ8R050
KOiK03267
OMAiIDVAHIY
OrthoDBiEOG091G0637
TreeFamiTF300566

Family and domain databases

InterProiView protein in InterPro
IPR009818 Ataxin-2_C
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PF07145 PAM2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R050-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPSSGGGGG GGGGGSSSSS DSAPDCWDQT DMEAPGPGPC GGGGSGSGSM
60 70 80 90 100
AAVAEAQREN LSAAFSRQLN VNAKPFVPNV HAAEFVPSFL RGPAQPPLSP
110 120 130 140 150
AGAAGGDHGA GSGAGGPSEP VESSQDQSCE GSNSTVSMEL SEPVVENGET
160 170 180 190 200
EMSPEESWEH KEEISEAEPG GGSSGDGRPP EESTQEMMEE EEEIPKPKSA
210 220 230 240 250
VAPPGAPKKE HVNVVFIGHV DAGKSTIGGQ IMYLTGMVDK RTLEKYEREA
260 270 280 290 300
KEKNRETWYL SWALDTNQEE RDKGKTVEVG RAYFETEKKH FTILDAPGHK
310 320 330 340 350
SFVPNMIGGA SQADLAVLVI SARKGEFETG FEKGGQTREH AMLAKTAGVK
360 370 380 390 400
HLIVLINKMD DPTVNWSNER YEECKEKLVP FLKKVGFNPK KDIHFMPCSG
410 420 430 440 450
LTGANLKEQS DFCPWYIGLP FIPYLDNLPN FNRSVDGPIR LPIVDKYKDM
460 470 480 490 500
GTVVLGKLES GSICKGQQLV MMPNKHNVEV LGILSDDVET DSVAPGENLK
510 520 530 540 550
IRLKGIEEEE ILPGFILCDL NNLCHSGRTF DAQIVIIEHK SIICPGYNAV
560 570 580 590 600
LHIHTCIEEV EITALICLVD KKSGEKSKTR PRFVKQDQVC IARLRTAGTI
610 620 630
CLETFKDFPQ MGRFTLRDEG KTIAIGKVLK LVPEKD
Length:636
Mass (Da):68,625
Last modified:June 13, 2012 - v2
Checksum:i28EC54B408D3F5A3
GO
Isoform 2 (identifier: Q8R050-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     145-145: Missing.

Show »
Length:635
Mass (Da):68,526
Checksum:i3EF70378C701C6BC
GO

Sequence cautioni

The sequence AAH28325 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH31640 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41G → A in AAH31640 (PubMed:15489334).Curated1
Sequence conflicti485S → C in BAA32526 (PubMed:9712840).Curated1
Sequence conflicti615T → R in BAA32526 (PubMed:9712840).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_043829145Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC087541 Genomic DNA No translation available.
CH466521 Genomic DNA Translation: EDK97345.1
BC028325 mRNA Translation: AAH28325.1 Different initiation.
BC031640 mRNA Translation: AAH31640.1 Different initiation.
AB003502 mRNA Translation: BAA32526.1
CCDSiCCDS27961.2 [Q8R050-1]
CCDS49764.1 [Q8R050-2]
RefSeqiNP_001123480.1, NM_001130008.1 [Q8R050-2]
NP_666178.2, NM_146066.2 [Q8R050-1]
UniGeneiMm.325827

Genome annotation databases

EnsembliENSMUST00000080030; ENSMUSP00000078940; ENSMUSG00000062203 [Q8R050-1]
ENSMUST00000167571; ENSMUSP00000130583; ENSMUSG00000062203 [Q8R050-2]
GeneIDi14852
KEGGimmu:14852
UCSCiuc007yfe.2 mouse [Q8R050-1]
uc007yff.2 mouse [Q8R050-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiERF3A_MOUSE
AccessioniPrimary (citable) accession number: Q8R050
Secondary accession number(s): E9QK49
, G3UWC0, O88179, Q8K2E1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: June 13, 2012
Last modified: May 23, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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