Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peptidyl-tRNA hydrolase ICT1, mitochondrial

Gene

Mrpl58

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit (PubMed:20869366). Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes (By similarity).By similarity1 Publication

Caution

In contrast to other members of the family, lacks the regions that come into close contact with the mRNA in the ribosomal A-site and determine the STOP codon specificity, explaining the loss of codon specificity for translation release factor activity.Curated

Catalytic activityi

N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Ribonucleoprotein, Ribosomal protein
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-5389840 Mitochondrial translation elongation
R-MMU-5419276 Mitochondrial translation termination

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-tRNA hydrolase ICT1, mitochondrial (EC:3.1.1.29)
Alternative name(s):
39S ribosomal protein L58, mitochondrialImported
Short name:
MRP-L58
Immature colon carcinoma transcript 1 protein homolog
Gene namesi
Name:Mrpl58Imported
Synonyms:Ict1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1915822 Mrpl58

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29MitochondrionSequence analysisAdd BLAST29
ChainiPRO_000003034030 – 206Peptidyl-tRNA hydrolase ICT1, mitochondrialAdd BLAST177

Proteomic databases

EPDiQ8R035
PeptideAtlasiQ8R035
PRIDEiQ8R035

PTM databases

iPTMnetiQ8R035
PhosphoSitePlusiQ8R035

Expressioni

Gene expression databases

BgeeiENSMUSG00000018858
CleanExiMM_ICT1
ExpressionAtlasiQ8R035 baseline and differential
GenevisibleiQ8R035 MM

Interactioni

Subunit structurei

Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins.By similarity

Structurei

Secondary structure

1206
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni74 – 76Combined sources3
Beta strandi77 – 82Combined sources6
Beta strandi87 – 90Combined sources4
Beta strandi98 – 104Combined sources7
Helixi105 – 107Combined sources3
Helixi113 – 122Combined sources10
Turni123 – 126Combined sources4
Beta strandi129 – 137Combined sources9
Helixi143 – 161Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J26NMR-A63-161[»]
ProteinModelPortaliQ8R035
SMRiQ8R035
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R035

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000013268
HOGENOMiHOG000231063
HOVERGENiHBG006115
InParanoidiQ8R035
KOiK15033
OMAiKIYPNAR
OrthoDBiEOG091G0SBR
PhylomeDBiQ8R035
TreeFamiTF315161

Family and domain databases

InterProiView protein in InterPro
IPR000352 Pep_chain_release_fac_I_II
PfamiView protein in Pfam
PF00472 RF-1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R035-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATAWGLRWG LSRTGTLLLA PPARCARRAL HRQVDGTTFQ SIYSLDKLYP
60 70 80 90 100
ESKGADTAWK VPEHAKQASS YIPLDRLSIS YCRSSGPGGQ NVNKVNSKAE
110 120 130 140 150
VRFHLASADW IEEPVRQKIA LTHKNKINKA GELVLTSESS RYQFRNLAEC
160 170 180 190 200
LQKIRDMIAE ASQVPKEPSK EDARLQRLRI EKMNRERLRQ KRLNSALKTS

RRMTMD
Length:206
Mass (Da):23,477
Last modified:June 1, 2002 - v1
Checksum:i1B898BE6A559E1F0
GO
Isoform 2 (identifier: Q8R035-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     34-62: Missing.

Note: No experimental confirmation available.
Show »
Length:177
Mass (Da):20,277
Checksum:iD23C97F02213C13D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01437434 – 62Missing in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003192 mRNA Translation: BAB22632.1
AK003286 mRNA Translation: BAB22691.1
AK160697 mRNA Translation: BAE35961.1
AL603828, AL627096 Genomic DNA Translation: CAM13810.1
AL627096, AL603828 Genomic DNA Translation: CAM22619.1
AL603828, AL627096 Genomic DNA Translation: CAM13811.1
AL627096, AL603828 Genomic DNA Translation: CAM22618.1
CH466558 Genomic DNA Translation: EDL34481.1
CH466558 Genomic DNA Translation: EDL34486.1
BC028523 mRNA Translation: AAH28523.1
CCDSiCCDS25632.1 [Q8R035-2]
CCDS83928.1 [Q8R035-1]
RefSeqiNP_001334574.1, NM_001347645.1 [Q8R035-1]
NP_081005.1, NM_026729.1 [Q8R035-2]
UniGeneiMm.271062

Genome annotation databases

EnsembliENSMUST00000103036; ENSMUSP00000099325; ENSMUSG00000018858 [Q8R035-2]
ENSMUST00000153983; ENSMUSP00000116746; ENSMUSG00000018858 [Q8R035-1]
GeneIDi68572
KEGGimmu:68572
UCSCiuc007mhj.1 mouse [Q8R035-1]
uc007mhl.1 mouse [Q8R035-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiICT1_MOUSE
AccessioniPrimary (citable) accession number: Q8R035
Secondary accession number(s): A2A6T2
, A2A6T3, Q3TUL0, Q9CTK1, Q9D1R3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health