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Protein

Bleomycin hydrolase

Gene

Blmh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.By similarity

Catalytic activityi

Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73PROSITE-ProRule annotation1
Active sitei372PROSITE-ProRule annotation1
Active sitei396PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • homocysteine catabolic process Source: GO_Central
  • response to drug Source: MGI
  • response to toxic substance Source: MGI

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.40 3474
ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Protein family/group databases

MEROPSiC01.084

Names & Taxonomyi

Protein namesi
Recommended name:
Bleomycin hydrolase (EC:3.4.22.40)
Short name:
BH
Short name:
BLM hydrolase
Short name:
BMH
Gene namesi
Name:Blmh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1345186 Blmh

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000505511 – 455Bleomycin hydrolaseAdd BLAST455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei391N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8R016
MaxQBiQ8R016
PaxDbiQ8R016
PeptideAtlasiQ8R016
PRIDEiQ8R016

PTM databases

iPTMnetiQ8R016
PhosphoSitePlusiQ8R016
SwissPalmiQ8R016

Expressioni

Gene expression databases

BgeeiENSMUSG00000020840
CleanExiMM_BLMH
ExpressionAtlasiQ8R016 baseline and differential
GenevisibleiQ8R016 MM

Interactioni

Subunit structurei

Homohexamer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi222434, 1 interactor
IntActiQ8R016, 2 interactors
MINTiQ8R016
STRINGi10090.ENSMUSP00000021197

Structurei

3D structure databases

ProteinModelPortaliQ8R016
SMRiQ8R016
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4128 Eukaryota
COG3579 LUCA
GeneTreeiENSGT00390000001735
HOGENOMiHOG000064089
HOVERGENiHBG002388
InParanoidiQ8R016
KOiK01372
OMAiGGQWDMI
OrthoDBiEOG091G07WT
PhylomeDBiQ8R016
TreeFamiTF323372

Family and domain databases

CDDicd00585 Peptidase_C1B, 1 hit
InterProiView protein in InterPro
IPR000169 Pept_cys_AS
IPR004134 Peptidase_C1B
PANTHERiPTHR10363 PTHR10363, 1 hit
PfamiView protein in Pfam
PF03051 Peptidase_C1_2, 1 hit
PIRSFiPIRSF005700 PepC, 1 hit
PROSITEiView protein in PROSITE
PS00139 THIOL_PROTEASE_CYS, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R016-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNAGLNSEK VSALIQKLNS DPQFVLAQNV GTTHDLLDIC LRRATVQGAQ
60 70 80 90 100
HVFQHVVPQE GKPVTNQKSS GRCWIFSCLN VMRLPFMKKF NIEEFEFSQS
110 120 130 140 150
YLFFWDKVER CYFFLNAFVD TAQKKEPEDG RLVQYLLMNP TNDGGQWDML
160 170 180 190 200
VNIVEKYGVV PKKCFPESHT TEATRRMNDI LNHKMREFCI RLRNLVHSGA
210 220 230 240 250
TKGEISSTQD AMMEEIFRVV CICLGNPPET FTWEYRDKDK NYHKIGPITP
260 270 280 290 300
LQFYKEHVKP LFNMEDKICF VNDPRPQHKY NKLYTVDYLS NMVGGRKTLY
310 320 330 340 350
NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNGKLGLSD MNVYDHELVF
360 370 380 390 400
GVSLKNMNKA ERLAFGESLM THAMTFTAVS EKDNQEGTFV KWRVENSWGE
410 420 430 440 450
DHGHKGYLCM TDEWFSEYVY EVVVDKKHVP EEVLAVLEQE PIVLPAWDPM

GALAE
Length:455
Mass (Da):52,511
Last modified:June 1, 2002 - v1
Checksum:i032F3B1DA1AD5063
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16Q → K in BAC28989 (PubMed:16141072).Curated1
Sequence conflicti151V → A in AAH24090 (PubMed:15489334).Curated1
Sequence conflicti391K → R in BAC30706 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK035228 mRNA Translation: BAC28989.1
AK040799 mRNA Translation: BAC30706.1
AK051441 mRNA Translation: BAC34639.1
AK053872 mRNA Translation: BAC35568.1
AK150438 mRNA Translation: BAE29560.1
AK167325 mRNA Translation: BAE39427.1
AL603842 Genomic DNA No translation available.
BC024090 mRNA Translation: AAH24090.1
BC027037 mRNA Translation: AAH27037.1
BC027362 mRNA Translation: AAH27362.1
BC027403 mRNA Translation: AAH27403.1
CCDSiCCDS25073.1
RefSeqiNP_848760.1, NM_178645.4
UniGeneiMm.399785

Genome annotation databases

EnsembliENSMUST00000021197; ENSMUSP00000021197; ENSMUSG00000020840
GeneIDi104184
KEGGimmu:104184
UCSCiuc007kge.2 mouse

Similar proteinsi

Entry informationi

Entry nameiBLMH_MOUSE
AccessioniPrimary (citable) accession number: Q8R016
Secondary accession number(s): Q3TJR8
, Q8BLZ4, Q8BZH9, Q8C111, Q8CID9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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