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Protein

Aminoacyl tRNA synthase complex-interacting multifunctional protein 2

Gene

Aimp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for assembly and stability of the aminoacyl-tRNA synthase complex (PubMed:12060739). Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down-regulation which is required for aveolar type II cell differentiation (PubMed:12819782). Blocks MDM2-mediated ubiquitination and degradation of p53/TP53 (PubMed:18695251). Functions as a proapoptotic factor (PubMed:16135753).4 Publications

GO - Molecular functioni

  • protein complex scaffold activity Source: CAFA

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • negative regulation of cell proliferation Source: MGI
  • positive regulation of aminoacyl-tRNA ligase activity Source: CAFA
  • positive regulation of neuron death Source: MGI
  • positive regulation of protein ubiquitination Source: MGI
  • protein complex assembly Source: CAFA
  • translation Source: UniProtKB-KW
  • Type II pneumocyte differentiation Source: MGI

Keywordsi

Molecular functionDevelopmental protein
Biological processApoptosis, Differentiation, Protein biosynthesis

Protein family/group databases

MoonProtiQ8R010.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoacyl tRNA synthase complex-interacting multifunctional protein 2
Alternative name(s):
Multisynthase complex auxiliary component p381 Publication
Protein JTV-1
Gene namesi
Name:Aimp2
Synonyms:Jtv1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2385237. Aimp2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Reduced levels of component enzymes and associated factors of the aminoacyl-tRNA synthase complex, lack of complex formation and lethality within two days of birth. Neonates display severe hyperplasia in a number of organs including lung, intestine and liver, lung failure, and disturbed thymocyte proliferation and differentiation. Embryonic fibroblasts deficient in Aimp2 are resistant to apoptosis following UV irradiation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003168411 – 320Aminoacyl tRNA synthase complex-interacting multifunctional protein 2Add BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on serine residues in response to UV irradiation.1 Publication
Ubiquitinated by PRKN, leading to its degradation by the proteasome.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8R010.
MaxQBiQ8R010.
PaxDbiQ8R010.
PeptideAtlasiQ8R010.
PRIDEiQ8R010.

PTM databases

iPTMnetiQ8R010.
PhosphoSitePlusiQ8R010.
SwissPalmiQ8R010.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029610.
CleanExiMM_JTV1.
ExpressionAtlasiQ8R010. baseline and differential.
GenevisibleiQ8R010. MM.

Interactioni

Subunit structurei

Part of a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 (PubMed:12060739). Interacts (via N-terminus) with KARS. Interacts with EPRS. Forms a linear complex that contains MARS, EEF1E1, EPRS and AIMP2 that is at the core of the multisubunit complex. Binds FUBP1 (via C-terminus) (By similarity). Interacts in both its unphosphorylated and phosphorylated forms with p53/TP53 (via N-terminus) in the nucleus following UV irradiation. Interacts (via N-terminus) with PRKN/parkin (via first RING-type domain).By similarity1 Publication

GO - Molecular functioni

  • protein complex scaffold activity Source: CAFA

Protein-protein interaction databases

BioGridi231187. 1 interactor.
IntActiQ8R010. 1 interactor.
MINTiMINT-1848800.
STRINGi10090.ENSMUSP00000031613.

Structurei

3D structure databases

ProteinModelPortaliQ8R010.
SMRiQ8R010.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini220 – 317GST C-terminalAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 162Interaction with PRKNBy similarityAdd BLAST81
Regioni162 – 225Interaction with TP53By similarityAdd BLAST64

Phylogenomic databases

eggNOGiENOG410II5D. Eukaryota.
ENOG4110B04. LUCA.
GeneTreeiENSGT00390000015826.
HOGENOMiHOG000015282.
InParanoidiQ8R010.
KOiK15438.
OMAiRVELPTC.
OrthoDBiEOG091G0Q05.
TreeFamiTF326322.

Family and domain databases

InterProiView protein in InterPro
IPR031889. AIMP2_LysRS-bd.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
PfamiView protein in Pfam
PF16780. AIMP2_LysRS_bd. 1 hit.
PF00043. GST_C. 1 hit.
SUPFAMiSSF47616. SSF47616. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8R010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPMYQVKPYH GGSAPLHVEL PTCMYRLPNV HSKTTSPATD AGHVQETSEP
60 70 80 90 100
SLQALESRQD DILKRLYELK AAVDGLSKMI HTPDADLDVT NILQADEPTT
110 120 130 140 150
LATNTLDLNS VLGKDYGALK DIVINANPAS PPLSLLVLHR LLCERYRVLS
160 170 180 190 200
TVHTHSSVKN VPENLVKCFG EQARKQSRHE YQLGFTLIWK NVPKTQMKFS
210 220 230 240 250
VQTMCPIEGE GNIARFLFSL FGQKHNAVTL TLIDSWVDIA MFQLREGSSK
260 270 280 290 300
EKAAVFRSMN SALGRSPWLV GNELTVADVV LWSVLQQTGG SSGAAPTNVQ
310 320
RWLKSCENLA PFSTALQLLK
Length:320
Mass (Da):35,378
Last modified:July 27, 2011 - v2
Checksum:i247EBE7067180170
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17H → R in AAH26958 (PubMed:15489334).Curated1
Sequence conflicti36S → N in AAH24410 (PubMed:15489334).Curated1
Sequence conflicti36S → N in AAH26958 (PubMed:15489334).Curated1
Sequence conflicti36S → N in AAH26972 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC121917 Genomic DNA. No translation available.
AC166900 Genomic DNA. No translation available.
BC024410 mRNA. Translation: AAH24410.1.
BC026958 mRNA. Translation: AAH26958.1.
BC026972 mRNA. Translation: AAH26972.1.
CCDSiCCDS51692.1.
RefSeqiNP_001165617.1. NM_001172146.1.
UniGeneiMm.200499.

Genome annotation databases

EnsembliENSMUST00000031613; ENSMUSP00000031613; ENSMUSG00000029610.
GeneIDi231872.
KEGGimmu:231872.
UCSCiuc009akw.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiAIMP2_MOUSE
AccessioniPrimary (citable) accession number: Q8R010
Secondary accession number(s): E9QP67, Q8R2Y6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 27, 2011
Last modified: September 27, 2017
This is version 100 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot