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Protein

Developmental pluripotency-associated protein 3

Gene

Dppa3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis.7 Publications

GO - Molecular functioni

  • methylated histone binding Source: UniProtKB

GO - Biological processi

  • chromatin modification Source: UniProtKB-KW
  • embryonic cleavage Source: MGI
  • negative regulation of DNA demethylation Source: UniProtKB
  • protection of DNA demethylation of female pronucleus Source: UniProtKB
  • regulation of genetic imprinting Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Developmental pluripotency-associated protein 3
Alternative name(s):
Compaction-associated protein 1
Primordial germ cell protein 7
Gene namesi
Name:Dppa3
Synonyms:Cap1p, Crg1, Pgc7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1920958. Dppa3.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Localized in the cytoplasm at the primary oocyte stage and in oocytes within mono-laminar follicles. Expressed in the nucleus and cytoplasm of oocytes in bi-laminar and Graafian follicles and during the 2-cell and morula stages. In E3.5 blastocysts localization is mainly nuclear. Mainly localizes in the female pronucleus, localization to the male pronucleus in much weaker.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • female pronucleus Source: UniProtKB
  • male pronucleus Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Null mutation result in apparently normal offspring. No effect on early gonadal PGCs or gross abnormalities in the development of gametes. However, females display severely reduced fertility despite ovulation of normal numbers of oocytes. Null mutation resulted in preimplantation development failure. Embryos rarely reached the blastocyst stage.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi44 – 441L → A: Abolishes localization to the nucleus and ability to prevent DNA demethylation; when associated with A-46. 1 Publication
Mutagenesisi46 – 461L → A: Abolishes localization to the nucleus and ability to prevent DNA demethylation; when associated with A-44. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 150150Developmental pluripotency-associated protein 3PRO_0000239266Add
BLAST

Proteomic databases

PaxDbiQ8QZY3.
PRIDEiQ8QZY3.

PTM databases

iPTMnetiQ8QZY3.
PhosphoSiteiQ8QZY3.

Expressioni

Tissue specificityi

Expressed in the immature oocytes and in newborn ovaries. Subsequently detected in maturing oocytes and in preimplantation embryos. Expressed in pluripotent embryonic but not in differentiated somatic cells. Expressed in blastocysts, epiblasts, primordial germ cells, embryonic gonads and primitive spermatogonia. No expression is detected in adult testes.3 Publications

Developmental stagei

Detected at E3.5 (at protein level). Activated during the process of germ cell specification at E7.25, specifically in the founder population of lineage-restricted primordial germ cells (PGCs). Thereafter, expressed in the germ line until about E15.5 in male and E13.5 in female gonads. Expressed during blastocyst, morula and 4-cell embryo stages.4 Publications

Gene expression databases

BgeeiENSMUSG00000046323.
CleanExiMM_DPPA3.
ExpressionAtlasiQ8QZY3. baseline and differential.
GenevisibleiQ8QZY3. MM.

Interactioni

GO - Molecular functioni

  • methylated histone binding Source: UniProtKB

Protein-protein interaction databases

DIPiDIP-59897N.
STRINGi10090.ENSMUSP00000062832.

Structurei

3D structure databases

ProteinModelPortaliQ8QZY3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7575Required for H3K9me2-bindingAdd
BLAST
Regioni76 – 15075Required to exclude TET3 from the maternal pronucleusAdd
BLAST

Domaini

Mediates binding to H3K9me2 via N-terminal region, while ability to exclude TET3 from the maternal pronucleus requires the C-terminal part.1 Publication

Phylogenomic databases

eggNOGiENOG410JJNY. Eukaryota.
ENOG41115BI. LUCA.
GeneTreeiENSGT00800000124303.
HOGENOMiHOG000293369.
HOVERGENiHBG081463.
InParanoidiQ8QZY3.
OMAiWNTEPFR.
OrthoDBiEOG091G154N.
PhylomeDBiQ8QZY3.
TreeFamiTF338511.

Family and domain databases

InterProiIPR029096. Dppa3.
[Graphical view]
PANTHERiPTHR31577. PTHR31577. 1 hit.
PfamiPF15549. PGC7_Stella. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8QZY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEPSEKVDP MKDPETPQKK DEEDALDDTD VLQPETLVKV MKKLTLNPGV
60 70 80 90 100
KRSARRRSLR NRIAAVPVEN KSEKIRREVQ SAFPKRRVRT LLSVLKDPIA
110 120 130 140 150
KMRRLVRIEQ RQKRLEGNEF ERDSEPFRCL CTFCHYQRWD PSENAKIGKN
Length:150
Mass (Da):17,670
Last modified:June 1, 2002 - v1
Checksum:i88B59E272F8FAD42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY082485 mRNA. Translation: AAM03317.1.
AB072734 mRNA. Translation: BAB86304.1.
AF490347 mRNA. Translation: AAO84505.1.
AY134859 mRNA. Translation: AAN12283.1.
AK135769 mRNA. Translation: BAE22650.1.
AK136263 mRNA. Translation: BAE22904.1.
AC158651 Genomic DNA. No translation available.
BC099433 mRNA. Translation: AAH99433.1.
BC100331 mRNA. Translation: AAI00332.1.
BC107340 mRNA. Translation: AAI07341.1.
BC107341 mRNA. Translation: AAI07342.1.
CCDSiCCDS20500.1.
RefSeqiNP_631964.1. NM_139218.1.
UniGeneiMm.27982.

Genome annotation databases

EnsembliENSMUST00000049644; ENSMUSP00000062832; ENSMUSG00000046323.
GeneIDi73708.
KEGGimmu:73708.
UCSCiuc009dpn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY082485 mRNA. Translation: AAM03317.1.
AB072734 mRNA. Translation: BAB86304.1.
AF490347 mRNA. Translation: AAO84505.1.
AY134859 mRNA. Translation: AAN12283.1.
AK135769 mRNA. Translation: BAE22650.1.
AK136263 mRNA. Translation: BAE22904.1.
AC158651 Genomic DNA. No translation available.
BC099433 mRNA. Translation: AAH99433.1.
BC100331 mRNA. Translation: AAI00332.1.
BC107340 mRNA. Translation: AAI07341.1.
BC107341 mRNA. Translation: AAI07342.1.
CCDSiCCDS20500.1.
RefSeqiNP_631964.1. NM_139218.1.
UniGeneiMm.27982.

3D structure databases

ProteinModelPortaliQ8QZY3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59897N.
STRINGi10090.ENSMUSP00000062832.

PTM databases

iPTMnetiQ8QZY3.
PhosphoSiteiQ8QZY3.

Proteomic databases

PaxDbiQ8QZY3.
PRIDEiQ8QZY3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049644; ENSMUSP00000062832; ENSMUSG00000046323.
GeneIDi73708.
KEGGimmu:73708.
UCSCiuc009dpn.1. mouse.

Organism-specific databases

CTDi359787.
MGIiMGI:1920958. Dppa3.

Phylogenomic databases

eggNOGiENOG410JJNY. Eukaryota.
ENOG41115BI. LUCA.
GeneTreeiENSGT00800000124303.
HOGENOMiHOG000293369.
HOVERGENiHBG081463.
InParanoidiQ8QZY3.
OMAiWNTEPFR.
OrthoDBiEOG091G154N.
PhylomeDBiQ8QZY3.
TreeFamiTF338511.

Miscellaneous databases

ChiTaRSiDppa3. mouse.
PROiQ8QZY3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000046323.
CleanExiMM_DPPA3.
ExpressionAtlasiQ8QZY3. baseline and differential.
GenevisibleiQ8QZY3. MM.

Family and domain databases

InterProiIPR029096. Dppa3.
[Graphical view]
PANTHERiPTHR31577. PTHR31577. 1 hit.
PfamiPF15549. PGC7_Stella. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPPA3_MOUSE
AccessioniPrimary (citable) accession number: Q8QZY3
Secondary accession number(s): F6S6F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.