Reviewed, UniProtKB/Swiss-Prot Q8QZY2 (GLCTK_MOUSE)

Names and origin

Protein names

Recommended name:
    Glycerate kinase
    EC=2.7.1.31

Gene names

Name:

Glyctk

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + (R)-glycerate = ADP + 3-phospho-(R)-glycerate.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed in the hippocampus, callus, brain, cerebellum, renal cortex interstitial cells, epithelium of interlobular bile duct and skeletal muscle. Ref.4
Sequence similarities	Belongs to the glycerate kinase type-2 family.
Sequence caution	The sequence AAP97704.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerate kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8QZY2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8QZY2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-127: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 523	523	Glycerate kinase		PRO_0000287194
Regions
	Compositional bias	94 – 97	4	Poly-Ala
Natural variations
	Alternative sequence	1 – 127	127	Missing in isoform 2.		VSP_025366
Experimental info
	Sequence conflict	136	1	V → I in BAC33747. Ref.2
	Sequence conflict	136	1	V → I in BAC34112. Ref.2
	Sequence conflict	136	1	V → I in BAC35110. Ref.2
	Sequence conflict	139	1	F → Y in AAP97704. Ref.1
	Sequence conflict	235	1	Q → H in BAC33747. Ref.2
	Sequence conflict	261	1	A → T in BAC33747. Ref.2
	Sequence conflict	261	1	A → T in BAC34112. Ref.2
	Sequence conflict	261	1	A → T in BAC35110. Ref.2
	Sequence conflict	338	1	Q → R in BAC34112. Ref.2
	Sequence conflict	396	1	A → V in BAC33747. Ref.2
	Sequence conflict	396	1	A → V in BAC34112. Ref.2
	Sequence conflict	396	1	A → V in BAC35110. Ref.2
	Sequence conflict	485	1	T → A in BAC33747. Ref.2
	Sequence conflict	485	1	T → A in BAC34112. Ref.2
	Sequence conflict	485	1	T → A in BAC35110. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 8EECD09476764FF2	FASTA	523	55,293
	10 20 30 40 50 60 MAAALQVLPC LLRAPSRPLL WGPPVARMTS GMALAEQARQ LFDSAVGAVQ PGPMLQRTLS 70 80 90 100 110 120 LDPSGRQLKV RDRTFQLREN LYLVGFGKAV LGMAAAAEEL LAQHLVQGVI SVPKGIRAAM 130 140 150 160 170 180 EHAGKKEMLL KPHSRVQVFE GAEDNLPDRD ALRAALTIQQ LAEGLTADDL LLVLISGGGS 190 200 210 220 230 240 ALLPAPIPPV TLEEKQMLTK LLAARGATIQ ELNTIRKALS QLKGGGLAQA AYPAQVISLI 250 260 270 280 290 300 LSDVIGDPLE VIASGPTVAS AHSVQDCLHI LNHYGLRAAL PRSVKTVLSR ADSDPHGPHT 310 320 330 340 350 360 CGHVLNVIIG SNSLALAEAQ RQAEVLGYHA MVLSTAMQGD VKRVARFYGL LARVAAAHLT 370 380 390 400 410 420 PSLAERPLEE EAELHQLAAE LQLPDLQLEE ALEAVAKAKG PVCLLAGGEP TVQLQGSGKG 430 440 450 460 470 480 GRNQELALHV GVELGRQPLG PIDVLFLSGG TDGQDGPTKV AGAWVMSDLI SQASAESLDI 490 500 510 520 ATSLTNNDSY TFFCRFRGGT HLLHTGLTGT NVMDVHLLIL HPQ
	Isoform 2. Checksum: 0B97A395C5202E15	FASTA	396	41,702
	10 20 30 40 50 60 MLLKPHSRVQ VFEGAEDNLP DRDALRAALT IQQLAEGLTA DDLLLVLISG GGSALLPAPI 70 80 90 100 110 120 PPVTLEEKQM LTKLLAARGA TIQELNTIRK ALSQLKGGGL AQAAYPAQVI SLILSDVIGD 130 140 150 160 170 180 PLEVIASGPT VASAHSVQDC LHILNHYGLR AALPRSVKTV LSRADSDPHG PHTCGHVLNV 190 200 210 220 230 240 IIGSNSLALA EAQRQAEVLG YHAMVLSTAM QGDVKRVARF YGLLARVAAA HLTPSLAERP 250 260 270 280 290 300 LEEEAELHQL AAELQLPDLQ LEEALEAVAK AKGPVCLLAG GEPTVQLQGS GKGGRNQELA 310 320 330 340 350 360 LHVGVELGRQ PLGPIDVLFL SGGTDGQDGP TKVAGAWVMS DLISQASAES LDIATSLTNN 370 380 390 DSYTFFCRFR GGTHLLHTGL TGTNVMDVHL LILHPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Zan Q., Guo J.H., Yu L. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Kidney.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Kidney and Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: FVB/N. Tissue: Kidney and Liver.
[4]	"Isolation and characterization of the human D-glyceric acidemia related glycerate kinase gene GLYCTK1 and its alternatively splicing variant GLYCTK2." Guo J.-H., Hexige S., Chen L., Zhou G.-J., Wang X., Jiang J.-M., Kong Y.-H., Ji G.-Q., Wu C.-Q., Zhao S.-Y., Yu L. DNA Seq. 17:1-7(2006) [PubMed: 16753811] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF455785 mRNA. Translation: AAP97704.1. Frameshift. AK049425 mRNA. Translation: BAC33747.1. AK050182 mRNA. Translation: BAC34112.1. AK052709 mRNA. Translation: BAC35110.1. BC025834 mRNA. Translation: AAH25834.1. BC025935 mRNA. Translation: AAH25935.1. BC030732 mRNA. Translation: AAH30732.1. BC033063 mRNA. Translation: AAH33063.1. BC036136 mRNA. Translation: AAH36136.1. Different initiation.
IPI	IPI00453581. IPI00845645.
RefSeq	NP_001034675.1. NP_777271.3.
UniGene	Mm.335420
3D structure databases
HSSP	HSSP built from PDB template 1O0U based on UniProtKB Q9X1S1.
ModBase	Search...
Proteomic databases
PRIDE	Q8QZY2.
Genome annotation databases
Ensembl	ENSMUSG00000020258. Mus musculus. [Contig view]
GeneID	235582.
KEGG	mmu:235582.
Organism-specific databases
MGI	MGI:2444085. Glyctk.
Phylogenomic databases
HOVERGEN	Q8QZY2.
Enzyme and pathway databases
BRENDA	2.7.1.31. 244.
Gene expression databases
ArrayExpress	Q8QZY2.
Bgee	Q8QZY2.
CleanEx	MM_GLYCTK.
Family and domain databases
InterPro	IPR007835. MOFRL. [Graphical view]
Pfam	PF05161. MOFRL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	382759.
SOURCE	Search...

Entry information

Entry name

GLCTK_MOUSE

Accession

Primary (citable) accession number: Q8QZY2
Secondary accession number(s): Q7TNX6 , Q8BQL0, Q8C717, Q8C7I3, Q8CI81, Q8K0R7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)