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Protein

Eukaryotic translation initiation factor 3 subunit L

Gene

Eif3l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

  • translational initiation Source: UniProtKB
  • viral translational termination-reinitiation Source: MGI

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-MMU-72649 Translation initiation complex formation
R-MMU-72689 Formation of a pool of free 40S subunits
R-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition
R-MMU-72706 GTP hydrolysis and joining of the 60S ribosomal subunit

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit LUniRule annotation
Short name:
eIF3lUniRule annotation
Alternative name(s):
66 kDa tyrosine-rich heat shock protein
67 kDa polymerase-associated factor
Eukaryotic translation initiation factor 3 subunit 6-interacting proteinUniRule annotation
Eukaryotic translation initiation factor 3 subunit E-interacting proteinUniRule annotation
HSP-66Y
PAF67
Gene namesi
Name:Eif3l
Synonyms:Eif3eip, Eif3s6ip, Paf67
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2386251 Eif3l

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation
ChainiPRO_00002974982 – 564Eukaryotic translation initiation factor 3 subunit LAdd BLAST563

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineUniRule annotationBy similarity1
Modified residuei465N6-acetyllysineCombined sources1
Modified residuei549N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8QZY1
MaxQBiQ8QZY1
PaxDbiQ8QZY1
PeptideAtlasiQ8QZY1
PRIDEiQ8QZY1

PTM databases

iPTMnetiQ8QZY1
PhosphoSitePlusiQ8QZY1
SwissPalmiQ8QZY1

Expressioni

Gene expression databases

BgeeiENSMUSG00000033047
GenevisibleiQ8QZY1 MM

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex may interact with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation may lead to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RRN3.UniRule annotation2 Publications

Protein-protein interaction databases

BioGridi2301766 interactors.
IntActiQ8QZY1 3 interactors.
MINTiQ8QZY1
STRINGi10090.ENSMUSP00000038839

Structurei

3D structure databases

ProteinModelPortaliQ8QZY1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini331 – 537PCIPROSITE-ProRule annotationAdd BLAST207

Sequence similaritiesi

Belongs to the eIF-3 subunit L family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3677 Eukaryota
ENOG410XPJT LUCA
GeneTreeiENSGT00390000000411
HOGENOMiHOG000265377
HOVERGENiHBG001289
InParanoidiQ8QZY1
KOiK15029
OMAiDAPMEDY
OrthoDBiEOG091G06M8
PhylomeDBiQ8QZY1
TreeFamiTF101523

Family and domain databases

Gene3Di1.25.40.101 hit
HAMAPiMF_03011 eIF3l, 1 hit
InterProiView protein in InterPro
IPR019382 eIF3l
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR13242 PTHR13242, 1 hit
PfamiView protein in Pfam
PF10255 Paf67, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8QZY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYPADDYES EAAYDPYAYP GDYDMHTGDP KQDLAYERQY EQQTYQVIPE
60 70 80 90 100
VIKNFIQYFH KTVSDLIDQK VYELQASRVS SDVIDQKVYE IQDIYENSWT
110 120 130 140 150
KLTERFFKNT PWPEAEAIAP QVGNDAVFLI LYKELYYRHI YAKVSGGPSL
160 170 180 190 200
EQRFESYYNY CNLFNYILNA DGPAPLELPN QWLWDIIDEF IYQFQSFSQY
210 220 230 240 250
RCKTAKKSEG EMDFLRSNPK VWNVHSVLNV LHSLVDKSNI NRQLEVYTSG
260 270 280 290 300
GDPESVAGEY GRHSLYKMLG YFSLVGLLRL HSLLGDYYQA IKVLENIELN
310 320 330 340 350
KKSMYSRVPE CQVTTYYYVG FAYLMMRRYQ DAIRVFANIL LYIQRTKSMF
360 370 380 390 400
QRTTYKYEMI NKQNEQMHAL LAIALTMYPM RIDESIHLQL REKYGDKMLR
410 420 430 440 450
MQKGDPQVYE ELFSYACPKF LSPVVPNYDN VHPNYHKEPF LQQLKVFSDE
460 470 480 490 500
VQQQAQLSTI RSFLKLYTTM PVAKLAGFLD LTEQEFRIQL LVFKHKMKNL
510 520 530 540 550
VWTSGISALD GEFQSASEVD FYIDKDMIHI ADTKVARRYG DFFIRQIHKF
560
EELNRTLKKM GQRP
Length:564
Mass (Da):66,613
Last modified:June 1, 2002 - v1
Checksum:i914324CB5046763D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149S → P in BAE39691 (PubMed:16141072).Curated1
Sequence conflicti215L → F in CAC84554 (Ref. 1) Curated1
Sequence conflicti253P → Q in BAE40238 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ310346 mRNA Translation: CAC84554.1
AB066095 Genomic DNA Translation: BAB85122.1
AK032936 mRNA Translation: BAC28090.1
AK075584 mRNA Translation: BAC35837.1
AK150895 mRNA Translation: BAE29941.1
AK151175 mRNA Translation: BAE30177.1
AK167640 mRNA Translation: BAE39691.1
AK168009 mRNA Translation: BAE39996.1
AK168295 mRNA Translation: BAE40238.1
BC024463 mRNA Translation: AAH24463.1
CCDSiCCDS27632.1
RefSeqiNP_660121.2, NM_145139.2
UniGeneiMm.206404

Genome annotation databases

EnsembliENSMUST00000040518; ENSMUSP00000038839; ENSMUSG00000033047
GeneIDi223691
KEGGimmu:223691
UCSCiuc007wsk.1 mouse

Similar proteinsi

Entry informationi

Entry nameiEIF3L_MOUSE
AccessioniPrimary (citable) accession number: Q8QZY1
Secondary accession number(s): Q3THF8, Q3TJ04, Q91YE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: June 1, 2002
Last modified: February 28, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome