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Q8QZR5 (ALAT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alanine aminotransferase 1

Short name=ALT1
EC=2.6.1.2
Alternative name(s):
Glutamate pyruvate transaminase 1
Short name=GPT 1
Glutamic--alanine transaminase 1
Glutamic--pyruvic transaminase 1
Gene names
Name:Gpt
Synonyms:Gpt1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles By similarity.

Catalytic activity

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactor

Pyridoxal phosphate By similarity.

Pathway

Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Mainly expressed in liver, intestine, colon and white adipose tissue. Ref.1

Sequence similarities

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandPyridoxal phosphate
   Molecular functionAminotransferase
Transferase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-alanine catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionL-alanine:2-oxoglutarate aminotransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

pyridoxal phosphate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 496495Alanine aminotransferase 1
PRO_0000123934

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue3141N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8QZR5 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 386AD33881DC7083

FASTA49655,143
        10         20         30         40         50         60 
MASQRNDRIQ ASRNGLKGKV LTLDTMNPCV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF 

        70         80         90        100        110        120 
TEVIRANIGD AQAMGQRPIT FFRQVLALCV YPNLLSSPDF PEDAKRRAER ILQACGGHSL 

       130        140        150        160        170        180 
GAYSISSGIQ PIREDVAQYI ERRDGGIPAD PNNIFLSTGA SDAIVTMLKL LVAGEGRART 

       190        200        210        220        230        240 
GVLIPIPQYP LYSAALAELD AVQVDYYLDE ERAWALDIAE LRRALCQARD RCCPRVLCVI 

       250        260        270        280        290        300 
NPGNPTGQVQ TRECIEAVIR FAFEEGLFLM ADEVYQDNVY AEGSQFHSFK KVLTEMGPPY 

       310        320        330        340        350        360 
ATQQELASFH SVSKGYMGEC GFRGGYVEVV NMDAEVQKQM AKLMSVRLCP PVPGQALMGM 

       370        380        390        400        410        420 
VVSPPTPSEP SFKQFQAERQ EVLAELAAKA KLTEQVFNEA PGIRCNPVQG AMYSFPQIQL 

       430        440        450        460        470        480 
PLKAVQRAQD LGLAPDMFFC LCLLEETGIC VVPGSGFGQQ EGTYHFRMTI LPPMEKLRVL 

       490 
LEKLRHFHAK FTHEYS 

« Hide

References

« Hide 'large scale' references
[1]"Murine alanine aminotransferase: cDNA cloning, functional expression, and differential gene regulation in mouse fatty liver."
Jadhao S.B., Yang R.-Z., Lin Q., Hu H., Anania F.A., Shuldiner A.R., Gong D.-W.
Hepatology 39:1297-1302(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]Erratum
Jadhao S.B., Yang R.-Z., Lin Q., Hu H., Anania F.A., Shuldiner A.R., Gong D.-W.
Hepatology 40:269-269(2004)
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC022625 mRNA. Translation: AAH22625.1.
BC026846 mRNA. Translation: AAH26846.1.
RefSeqNP_877957.1. NM_182805.2.
UniGeneMm.30130.

3D structure databases

ProteinModelPortalQ8QZR5.
SMRQ8QZR5. Positions 21-495.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8QZR5. 2 interactions.
MINTMINT-1869747.

Proteomic databases

PaxDbQ8QZR5.
PRIDEQ8QZR5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023203; ENSMUSP00000023203; ENSMUSG00000022546.
GeneID76282.
KEGGmmu:76282.
UCSCuc007wls.1. mouse.

Organism-specific databases

CTD2875.
MGIMGI:95802. Gpt.

Phylogenomic databases

eggNOGCOG0436.
GeneTreeENSGT00650000093331.
HOGENOMHOG000215020.
HOVERGENHBG026148.
InParanoidQ8QZR5.
KOK00814.
OMALKLMSVR.
OrthoDBEOG76HQ18.
PhylomeDBQ8QZR5.
TreeFamTF300839.

Enzyme and pathway databases

UniPathwayUPA00528; UER00586.

Gene expression databases

ArrayExpressQ8QZR5.
BgeeQ8QZR5.
CleanExMM_GPT.
GenevestigatorQ8QZR5.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
ProtoNetSearch...

Other

NextBio344903.
PROQ8QZR5.
SOURCESearch...

Entry information

Entry nameALAT1_MOUSE
AccessionPrimary (citable) accession number: Q8QZR5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot