Reviewed, UniProtKB/Swiss-Prot Q8QMV9 (MCEL_CWPXB)

Names and origin

Protein names

Recommended name:
    mRNA-capping enzyme large subunit
Including the following 2 domains:
    1- Recommended name:
            Polynucleotide 5'-triphosphatase
              EC=3.1.3.33
        Alternative name(s):
            mRNA 5'-triphosphatase
              Short name=TPase
    2- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.50
        Alternative name(s):
            GTP--RNA guanylyltransferase
              Short name=GTase

Gene names

Ordered Locus Names:

CPXV118

Organism

Cowpox virus (strain Brighton Red) (CPV)

Taxonomic identifier

265872 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
Loxodonta africana (African elephant) [TaxID: 9785]
Bos taurus (Bovine) [TaxID: 9913]
Mus musculus (Mouse) [TaxID: 10090]
Microtus agrestis (Short-tailed field vole) [TaxID: 29092]
Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135]

Protein attributes

Sequence length	844 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the first two reactions in the mRNA cap formation pathway.
Catalytic activity	A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate. GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
Subunit structure	Heterodimer of a large and a small subunit By similarity.
Sequence similarities	Belongs to the viral GTase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC polynucleotide 5'-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 844	844	mRNA-capping enzyme large subunit		PRO_0000210128
Sites
	Active site	260	1	N6-GMP-lysine intermediate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8QMV9-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C760E4B77B43A3E8	FASTA	844	96,703
	10 20 30 40 50 60 MDANVVSSST IATYIDALAK NASELEQGST AYEINNELEL VFIKPPLITL TNVVNISTIQ 70 80 90 100 110 120 ESFIRFTVTN KEGVKIRTKI PLSKVHGLDV KNVQLVDAID NIVWEKKSLV TENRLHKECL 130 140 150 160 170 180 LRLSTEERHI FLDYKKYGSS IRLELVNLIQ AKTKNFTIDF KLKYFLGSGA QSKSSLLHAI 190 200 210 220 230 240 NHPKSRPNTS LEIEFTPRDN EKVPYDELIK ELTTLSRHIF MASPENVILS PPINPPIKTF 250 260 270 280 290 300 MLPKQDIVGL DLENLYAVTK TDGIPITIRV TSKGLYCYFT HLGYIIRYPV KRIIDSEVVV 310 320 330 340 350 360 FGEAVKDKNW TVYLIKLIEP VNAISDRLEE SKYVESKLVD ICDRIVFKSK KYEGPFTTTS 370 380 390 400 410 420 EVVDMLSTYL PKQPEGVILF YSKGPKSNID FKIKKENTID QTANVVFRYM SSEPIIFGES 430 440 450 460 470 480 SIFIEYKKFT NDKGFPKEYG SGKIVLYNGV NYLNNIYCLE YINTHNEVGI KSVVVPIKFI 490 500 510 520 530 540 AEFLVNGEIL KPRIDKTMKY INSEDYYGNQ HNVIVEHLRD QSIKIGDVFN EDKLSDVGHQ 550 560 570 580 590 600 YANNDKFRLN PEVSYFTNKR TRGPLGILSN YVKTLLISMY CSKTFLDDSN KRKVLAIDFG 610 620 630 640 650 660 NGADLEKYFY GEIALLVATD PDADAIARGN ERYNKLNSGI KTKYYKFDYI QETIRSDTFV 670 680 690 700 710 720 SSVREVFYFG KFNIIDWQFA IHYSFHPRHY ATVMNNLSEL TASGGKVLIT TMDGDKLSKL 730 740 750 760 770 780 TDKKTFIIHK NLPSSENYMS VEKIADDRIL VYNPSTMSTP MTEYIIKKND IVRVFNEYGF 790 800 810 820 830 840 VLVDNIDFAT IIERSKKFIN GASTMEDRPS TRNFFELNRG AIKCEGLDVE DLLSYYVVYV FSKR

References

[1]	Dietrich F.S., Ray C.A., Sharma D.A., Allen A., Pickup D.J. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF482758 Genomic DNA. Translation: AAM13560.1.
RefSeq	NP_619902.1.
3D structure databases
SMR	Q8QMV9. Positions 545-844.
ModBase	Search...
Genome annotation databases
GeneID	1485995.
Family and domain databases
InterPro	IPR019602. mRNA_cap_ATPase/GuylTrfase_vir. IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF10640. Pox_ATPase-GT. 1 hit. PF03291. Pox_MCEL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCEL_CWPXB

Accession

Primary (citable) accession number: Q8QMV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	June 1, 2002
Last modified:	January 19, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)