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Q8QHL3 (VGFR1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vascular endothelial growth factor receptor 1

Short name=VEGFR-1
EC=2.7.10.1
Gene names
Name:FLT1
Synonyms:VEGFR1
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length1327 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the regulation of angiogenesis, cell survival, cell migration, macrophage function, and chemotaxis. Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates PLCG1. Promotes phosphorylation of AKT1 and CBL By similarity.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulation

Present in an inactive conformation in the absence of bound ligand. Binding of VEGFA, VEGFB or PGF leads to dimerization and activation by autophosphorylation on tyrosine residues By similarity.

Subunit structure

Interacts with VEGFA, VEGFB and PGF. Monomer in the absence of bound VEGFA, VEGFB or PGF. Homodimer in the presence of bound VEGFA, VEGFB and PGF By similarity.

Subcellular location

Isoform 1: Cell membrane; Single-pass type I membrane protein. Endosome By similarity. Note: Autophosphorylation promotes internalization and degradation By similarity.

Isoform 2: Secreted.

Post-translational modification

Autophosphorylated on tyrosine residues upon ligand binding By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8QHL3-1)

Also known as: Flt1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8QHL3-2)

Also known as: sFlt1;

The sequence of this isoform differs from the canonical sequence as follows:
     647-677: AQEAPALLRQLMDQTVNTSNSAMLECQVHGI → GEHCNKKAVYSRILKYKNTRNDCTTQSNVKH
     678-1327: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 13271303Vascular endothelial growth factor receptor 1
PRO_0000249462

Regions

Topological domain25 – 749725Extracellular Potential
Transmembrane750 – 77021Helical; Potential
Topological domain771 – 1327557Cytoplasmic Potential
Domain30 – 12192Ig-like C2-type 1
Domain120 – 222103Ig-like C2-type 2
Domain227 – 32397Ig-like C2-type 3
Domain331 – 41787Ig-like C2-type 4
Domain424 – 545122Ig-like C2-type 5
Domain552 – 64493Ig-like C2-type 6
Domain651 – 73787Ig-like C2-type 7
Domain819 – 1151333Protein kinase
Nucleotide binding825 – 8339ATP By similarity

Sites

Active site10151Proton acceptor By similarity
Binding site8531ATP By similarity

Amino acid modifications

Modified residue10461Phosphotyrosine; by autocatalysis By similarity
Modified residue11621Phosphotyrosine; by autocatalysis By similarity
Modified residue12021Phosphotyrosine; by autocatalysis By similarity
Modified residue12311Phosphotyrosine; by autocatalysis By similarity
Modified residue13161Phosphotyrosine; by autocatalysis By similarity
Modified residue13221Phosphotyrosine; by autocatalysis By similarity
Glycosylation481N-linked (GlcNAc...) Potential
Glycosylation731N-linked (GlcNAc...) Potential
Glycosylation821N-linked (GlcNAc...) Potential
Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation1251N-linked (GlcNAc...) Potential
Glycosylation2471N-linked (GlcNAc...) Potential
Glycosylation3191N-linked (GlcNAc...) Potential
Glycosylation3831N-linked (GlcNAc...) Potential
Glycosylation3981N-linked (GlcNAc...) Potential
Glycosylation4091N-linked (GlcNAc...) Potential
Glycosylation4131N-linked (GlcNAc...) Potential
Glycosylation4701N-linked (GlcNAc...) Potential
Glycosylation5121N-linked (GlcNAc...) Potential
Glycosylation5431N-linked (GlcNAc...) Potential
Glycosylation5931N-linked (GlcNAc...) Potential
Glycosylation6151N-linked (GlcNAc...) Potential
Glycosylation6631N-linked (GlcNAc...) Potential
Disulfide bond51 ↔ 105 By similarity
Disulfide bond154 ↔ 203 By similarity
Disulfide bond248 ↔ 307 By similarity
Disulfide bond450 ↔ 531 By similarity
Disulfide bond573 ↔ 626 By similarity
Disulfide bond672 ↔ 721 By similarity

Natural variations

Alternative sequence647 – 67731AQEAP…QVHGI → GEHCNKKAVYSRILKYKNTR NDCTTQSNVKH in isoform 2.
VSP_020427
Alternative sequence678 – 1327650Missing in isoform 2.
VSP_027160

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Flt1) [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 96352C59348DC22D

FASTA1,327149,426
        10         20         30         40         50         60 
MPRQLLSGTV LLGAAFLLAG STSGSKLKVP VLSVNGRQHV VQAGQTLNLT CRGEMLHSWS 

        70         80         90        100        110        120 
LPEALSKDSK RLNVTKYACG RNGTQFCSTL TLSRTQANDT GRYSCRYPTS PVKKKRESIV 

       130        140        150        160        170        180 
YVFINDTSNP FVEMHSDIPK IIHMTVGKEM IIPCRVTAPN IAVTLKKIPR ETLIPDGKTI 

       190        200        210        220        230        240 
IWDNMRGFRI PEATYRFIGL LSCETTIGGH KYSTKYLTHR ETNTIFDIKL STPRLVKLLK 

       250        260        270        280        290        300 
GDSLAINCTV KAAWNTRVQM TWTYPGEAMK RGSVTQRIDQ KNREANVFYS ILVIDKVRDI 

       310        320        330        340        350        360 
DKGQYACHVK SGPSNKLVNT TVIVYDKRFI NLKRRRKTML EAVAGRKSYR LPMKVKAFPS 

       370        380        390        400        410        420 
PEVTWLKDGL PAAEKCARYM VKNYSLIIKD VAEEDAGNYT IILSLRQWNL SKNLTVTLKV 

       430        440        450        460        470        480 
NVKPQIYENA VSSFPDPNLY LLSSKQVLTC TVYGIPPPKI TWMWYPCRQN HSKTRRGFCS 

       490        500        510        520        530        540 
RTDGSFNLKT GSNIGNRIQS IIERTAIIEG KNKTASTLVV AEAKSSGIYS CVASNKVGKA 

       550        560        570        580        590        600 
ERNVSFLVTD VPSGFHISLE KVPIEGENLV LSCSANKFMY KDISWILPRT VTNQTKARKA 

       610        620        630        640        650        660 
LNKEYSITLT LTIRNVSLAH SGTYTCRARN IFTGKEVLQK KDVSIRAQEA PALLRQLMDQ 

       670        680        690        700        710        720 
TVNTSNSAML ECQVHGIPEP QITWFKNHEE IQQESGIILG PGSRMLFIER VKEEDEGLYQ 

       730        740        750        760        770        780 
CIATNLKGSV ESTAYVTVQG TVERSNLELI TLTCTCVAAT LFWLLLTLFI RKLKRPYFSE 

       790        800        810        820        830        840 
TKTNHYLSII MDPDEVPLDE QCECLPYDAS KWEIARERLK LGKSLGHGAF GKVVQASAFG 

       850        860        870        880        890        900 
IKKSPTCRIV AVKMLKEGAT ASEYKALMTE LKILIHIGHH LNIVNLLGAC TKNGGPLMVI 

       910        920        930        940        950        960 
VEYCKYGNLS NYLKSKRNFF SPTKDPSLQG ELMKDKKGIE PVEGKKQRLA SVTSSESFAS 

       970        980        990       1000       1010       1020 
SGFQEDKSLS DAEEDEEDAA ELYKLPLTME DLISYSFQVA RGMEFLSSRK CIHRDLAARN 

      1030       1040       1050       1060       1070       1080 
ILLSENNVVK ICDFGLARDI YKNPDYVRKG DARLPLKWMA PESIFDKIYN TKSDVWSYGV 

      1090       1100       1110       1120       1130       1140 
LLWEIFSLGA SPYPGVQIDE DFCSKLKEGT RMRAPEQATE EIYQIMLDCW RSNPNERPWF 

      1150       1160       1170       1180       1190       1200 
SELVKRLGDL LQASVQQEGK DYIPLDTIFT AESGFPPASD PLCNEKFPVP SPNCRSTERA 

      1210       1220       1230       1240       1250       1260 
RYINTFKIKP PQRIKTFEEL PIKEKLVFND YQADSGMVLA SEELKRFTWT GSKQKWTLFG 

      1270       1280       1290       1300       1310       1320 
MKGVSRSKES GLSGITKPRS FCSFSCDQLS ESKRRYTYGN TVLEKMKACH SPPPDYSSVV 


HYSQPSI 

« Hide

Isoform 2 (sFlt1) [UniParc].

Checksum: 31C4DC467D3243C4
Show »

FASTA67776,096

References

[1]"Soluble Flt-1 (soluble VEGFR-1), a potent natural antiangiogenic molecule in mammals, is phylogenetically conserved in avians."
Yamaguchi S., Iwata K., Shibuya M.
Biochem. Biophys. Res. Commun. 291:554-559(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB065372 mRNA. Translation: BAB84690.1.
AB065373 mRNA. Translation: BAB84691.1.
RefSeqNP_989583.1. NM_204252.1.
UniGeneGga.150.

3D structure databases

ProteinModelPortalQ8QHL3.
SMRQ8QHL3. Positions 125-225, 801-1157.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ8QHL3.
PRIDEQ8QHL3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID374100.
KEGGgga:374100.

Organism-specific databases

CTD2321.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000037949.
HOVERGENHBG053432.
InParanoidQ8QHL3.
KOK05096.
PhylomeDBQ8QHL3.

Family and domain databases

Gene3D2.60.40.10. 7 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR001824. Tyr_kinase_rcpt_3_CS.
IPR009135. VEGFR1_rcpt.
[Graphical view]
PANTHERPTHR24416:SF126. PTHR24416:SF126. 1 hit.
PfamPF07679. I-set. 3 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSPR01833. VEGFRECEPTR1.
SMARTSM00409. IG. 4 hits.
SM00408. IGc2. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 2 hits.
PROSITEPS50835. IG_LIKE. 6 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20813619.

Entry information

Entry nameVGFR1_CHICK
AccessionPrimary (citable) accession number: Q8QHL3
Secondary accession number(s): Q8QHL2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families