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Q8QHK8

- MK08_XENLA

UniProt

Q8QHK8 - MK08_XENLA

Protein

Mitogen-activated protein kinase 8

Gene

mapk8

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (30 Apr 2003)
      Previous versions | rss
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    Functioni

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, and thus regulating transcriptional activity By similarity. Regulates morphogenic cell movements, controlling convergent extension during gastrulation.By similarity1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.1 Publication

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation, potentially by the dual-specificity kinase, MKK7. Indirectly activated by Wnt5a.By similarity1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei55 – 551ATPPROSITE-ProRule annotation
    Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi33 – 386ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. JNK cascade Source: UniProtKB
    2. multicellular organismal development Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24. 6726.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 8 (EC:2.7.11.24)
    Short name:
    MAP kinase 8
    Short name:
    MAPK 8
    Alternative name(s):
    Stress-activated protein kinase JNK1
    Gene namesi
    Name:mapk8
    Synonyms:jnk1
    OrganismiXenopus laevis (African clawed frog)Imported
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-1217560. mapk8.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 426426Mitogen-activated protein kinase 8PRO_0000186268Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei183 – 1831PhosphothreonineBy similarity
    Modified residuei185 – 1851PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Tissue specificityi

    Strongly expressed in presumptive ectoderm and mesoderm regions and weakly expressed in endoderm regions during early stages of embryo development. Expressed in the head and dorsal regions during neurula and tailbud stages.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi98118. 1 interaction.
    IntActiQ8QHK8. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8QHK8.
    SMRiQ8QHK8. Positions 7-362.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 321296Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi183 – 1853TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG014652.
    KOiK04440.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008351. MAPK_JNK.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01772. JNKMAPKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 2 (identifier: Q8QHK8-1) [UniParc]FASTAAdd to Basket

    Also known as: Beta

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSRSKRDSNF SVFEIGDSTF TVLKRYQNLK PIGSGAQGIV CAAFDAVLER    50
    HVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKSLEE 100
    FQDLYIVMEL MDANLCQVIQ MELDHERMSY LLYQMLCGIK HLHSAGIIHR 150
    DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM 200
    GYKENVDIWS VGCILGEMIK GGVLFPGTDH IDQWNKVIEQ LGTPCTEFMK 250
    KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK 300
    MLVIDASKRI SVDDALQHPY INVWYDPLEA EAPPPKIPDK QLDEREHTIE 350
    EWKELIYKEV LDWEERAKNG VIRGQPAPLG AAVTDGSQAH TSSSSGDASS 400
    MSTDPTLPSD TDSSLETSAG TLGCCR 426
    Length:426
    Mass (Da):47,928
    Last modified:April 30, 2003 - v2
    Checksum:i51990EC50A556092
    GO
    Isoform 1 (identifier: Q8QHK8-2) [UniParc]FASTAAdd to Basket

    Also known as: Alpha

    The sequence of this isoform differs from the canonical sequence as follows:
         380-384: GAAVT → AQVQQ
         385-426: Missing.

    Show »
    Length:384
    Mass (Da):43,993
    Checksum:i77078A6C4CEE0AC8
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei380 – 3845GAAVT → AQVQQ in isoform 1. 1 PublicationVSP_007348
    Alternative sequencei385 – 42642Missing in isoform 1. 1 PublicationVSP_007349Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB073999 mRNA. Translation: BAB85483.1.
    AB074000 mRNA. Translation: BAB91438.1.
    BC046834 mRNA. Translation: AAH46834.1.
    RefSeqiNP_001080184.1. NM_001086715.1. [Q8QHK8-1]
    UniGeneiXl.8740.

    Genome annotation databases

    GeneIDi379876.
    KEGGixla:379876.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB073999 mRNA. Translation: BAB85483.1 .
    AB074000 mRNA. Translation: BAB91438.1 .
    BC046834 mRNA. Translation: AAH46834.1 .
    RefSeqi NP_001080184.1. NM_001086715.1. [Q8QHK8-1 ]
    UniGenei Xl.8740.

    3D structure databases

    ProteinModelPortali Q8QHK8.
    SMRi Q8QHK8. Positions 7-362.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 98118. 1 interaction.
    IntActi Q8QHK8. 1 interaction.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 379876.
    KEGGi xla:379876.

    Organism-specific databases

    CTDi 5599.
    Xenbasei XB-GENE-1217560. mapk8.

    Phylogenomic databases

    HOVERGENi HBG014652.
    KOi K04440.

    Enzyme and pathway databases

    BRENDAi 2.7.11.24. 6726.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008351. MAPK_JNK.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01772. JNKMAPKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "JNK functions in the non-canonical Wnt pathway to regulate convergent extension movements in vertebrates."
      Yamanaka H., Moriguchi T., Masuyama N., Kusakabe M., Hanafusa H., Takada R., Takada S., Nishida E.
      EMBO Rep. 3:69-75(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, COFACTOR, TISSUE SPECIFICITY, ENZYME REGULATION.
      Tissue: Oocyte.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo.

    Entry informationi

    Entry nameiMK08_XENLA
    AccessioniPrimary (citable) accession number: Q8QHK8
    Secondary accession number(s): Q8JJC3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3