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Q8QHK8 (MK08_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 8

Short name=MAP kinase 8
Short name=MAPK 8
EC=2.7.11.24
Alternative name(s):
Stress-activated protein kinase JNK1
Gene names
Name:mapk8
Synonyms:jnk1
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, and thus regulating transcriptional activity By similarity. Regulates morphogenic cell movements, controlling convergent extension during gastrulation. Ref.1 UniProtKB P45983

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium. Ref.1

Enzyme regulation

Activated by threonine and tyrosine phosphorylation, potentially by the dual-specificity kinase, MKK7. Indirectly activated by Wnt5a. Ref.1 UniProtKB P45983

Tissue specificity

Strongly expressed in presumptive ectoderm and mesoderm regions and weakly expressed in endoderm regions during early stages of embryo development. Expressed in the head and dorsal regions during neurula and tailbud stages. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: Q8QHK8-1)

Also known as: Beta;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: Q8QHK8-2)

Also known as: Alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     380-384: GAAVT → AQVQQ
     385-426: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 426426Mitogen-activated protein kinase 8
PRO_0000186268

Regions

Domain26 – 321296Protein kinase UniProtKB P53779
Nucleotide binding33 – 386ATP By similarity UniProtKB P53779
Motif183 – 1853TXY

Sites

Active site1511Proton acceptor By similarity UniProtKB P53779
Binding site551ATP By similarity UniProtKB P53779

Amino acid modifications

Modified residue1831Phosphothreonine By similarity UniProtKB P53779
Modified residue1851Phosphotyrosine By similarity UniProtKB P53779

Natural variations

Alternative sequence380 – 3845GAAVT → AQVQQ in isoform 1.
VSP_007348
Alternative sequence385 – 42642Missing in isoform 1.
VSP_007349

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 (Beta) [UniParc].

Last modified April 30, 2003. Version 2.
Checksum: 51990EC50A556092

FASTA42647,928
        10         20         30         40         50         60 
MSRSKRDSNF SVFEIGDSTF TVLKRYQNLK PIGSGAQGIV CAAFDAVLER HVAIKKLSRP 

        70         80         90        100        110        120 
FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKSLEE FQDLYIVMEL MDANLCQVIQ 

       130        140        150        160        170        180 
MELDHERMSY LLYQMLCGIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAGTSF 

       190        200        210        220        230        240 
MMTPYVVTRY YRAPEVILGM GYKENVDIWS VGCILGEMIK GGVLFPGTDH IDQWNKVIEQ 

       250        260        270        280        290        300 
LGTPCTEFMK KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK 

       310        320        330        340        350        360 
MLVIDASKRI SVDDALQHPY INVWYDPLEA EAPPPKIPDK QLDEREHTIE EWKELIYKEV 

       370        380        390        400        410        420 
LDWEERAKNG VIRGQPAPLG AAVTDGSQAH TSSSSGDASS MSTDPTLPSD TDSSLETSAG 


TLGCCR 

« Hide

Isoform 1 (Alpha) [UniParc].

Checksum: 77078A6C4CEE0AC8
Show »

FASTA38443,993

References

« Hide 'large scale' references
[1]"JNK functions in the non-canonical Wnt pathway to regulate convergent extension movements in vertebrates."
Yamanaka H., Moriguchi T., Masuyama N., Kusakabe M., Hanafusa H., Takada R., Takada S., Nishida E.
EMBO Rep. 3:69-75(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, COFACTOR, TISSUE SPECIFICITY, ENZYME REGULATION.
Tissue: Oocyte.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB073999 mRNA. Translation: BAB85483.1.
AB074000 mRNA. Translation: BAB91438.1.
BC046834 mRNA. Translation: AAH46834.1.
RefSeqNP_001080184.1. NM_001086715.1. [Q8QHK8-1]
UniGeneXl.8740.

3D structure databases

ProteinModelPortalQ8QHK8.
SMRQ8QHK8. Positions 7-362.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid98118. 1 interaction.
IntActQ8QHK8. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID379876.
KEGGxla:379876.

Organism-specific databases

CTD5599.
XenbaseXB-GENE-1217560. mapk8.

Phylogenomic databases

HOVERGENHBG014652.
KOK04440.

Enzyme and pathway databases

BRENDA2.7.11.24. 6726.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01772. JNKMAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMK08_XENLA
AccessionPrimary (citable) accession number: Q8QHK8
Secondary accession number(s): Q8JJC3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: May 14, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families