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Protein

Mitogen-activated protein kinase 8

Gene

mapk8

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, and thus regulating transcriptional activity (By similarity). Regulates morphogenic cell movements, controlling convergent extension during gastrulation. May play a role in the regulation of the circadian clock (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+1 Publication

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation, potentially by the dual-specificity kinase, MKK7. Indirectly activated by Wnt5a.By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551ATPPROSITE-ProRule annotation
Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 386ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. JNK cascade Source: UniProtKB
  2. multicellular organismal development Source: UniProtKB-KW
  3. rhythmic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Biological rhythms

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 6725.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 8 (EC:2.7.11.24)
Short name:
MAP kinase 8
Short name:
MAPK 8
Alternative name(s):
Stress-activated protein kinase JNK1
Gene namesi
Name:mapk8
Synonyms:jnk1
OrganismiXenopus laevis (African clawed frog)Imported
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-1217560. mapk8.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426Mitogen-activated protein kinase 8PRO_0000186268Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei183 – 1831PhosphothreonineBy similarity
Modified residuei185 – 1851PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Strongly expressed in presumptive ectoderm and mesoderm regions and weakly expressed in endoderm regions during early stages of embryo development. Expressed in the head and dorsal regions during neurula and tailbud stages.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi98118. 1 interaction.
IntActiQ8QHK8. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ8QHK8.
SMRiQ8QHK8. Positions 7-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 321296Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi183 – 1853TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG014652.
KOiK04440.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01772. JNKMAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: Q8QHK8-1) [UniParc]FASTAAdd to basket

Also known as: Beta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRSKRDSNF SVFEIGDSTF TVLKRYQNLK PIGSGAQGIV CAAFDAVLER
60 70 80 90 100
HVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKSLEE
110 120 130 140 150
FQDLYIVMEL MDANLCQVIQ MELDHERMSY LLYQMLCGIK HLHSAGIIHR
160 170 180 190 200
DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM
210 220 230 240 250
GYKENVDIWS VGCILGEMIK GGVLFPGTDH IDQWNKVIEQ LGTPCTEFMK
260 270 280 290 300
KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK
310 320 330 340 350
MLVIDASKRI SVDDALQHPY INVWYDPLEA EAPPPKIPDK QLDEREHTIE
360 370 380 390 400
EWKELIYKEV LDWEERAKNG VIRGQPAPLG AAVTDGSQAH TSSSSGDASS
410 420
MSTDPTLPSD TDSSLETSAG TLGCCR
Length:426
Mass (Da):47,928
Last modified:April 30, 2003 - v2
Checksum:i51990EC50A556092
GO
Isoform 1 (identifier: Q8QHK8-2) [UniParc]FASTAAdd to basket

Also known as: Alpha

The sequence of this isoform differs from the canonical sequence as follows:
     380-384: GAAVT → AQVQQ
     385-426: Missing.

Show »
Length:384
Mass (Da):43,993
Checksum:i77078A6C4CEE0AC8
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei380 – 3845GAAVT → AQVQQ in isoform 1. 1 PublicationVSP_007348
Alternative sequencei385 – 42642Missing in isoform 1. 1 PublicationVSP_007349Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073999 mRNA. Translation: BAB85483.1.
AB074000 mRNA. Translation: BAB91438.1.
BC046834 mRNA. Translation: AAH46834.1.
RefSeqiNP_001080184.1. NM_001086715.1. [Q8QHK8-1]
UniGeneiXl.8740.

Genome annotation databases

GeneIDi379876.
KEGGixla:379876.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073999 mRNA. Translation: BAB85483.1.
AB074000 mRNA. Translation: BAB91438.1.
BC046834 mRNA. Translation: AAH46834.1.
RefSeqiNP_001080184.1. NM_001086715.1. [Q8QHK8-1]
UniGeneiXl.8740.

3D structure databases

ProteinModelPortaliQ8QHK8.
SMRiQ8QHK8. Positions 7-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi98118. 1 interaction.
IntActiQ8QHK8. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi379876.
KEGGixla:379876.

Organism-specific databases

CTDi5599.
XenbaseiXB-GENE-1217560. mapk8.

Phylogenomic databases

HOVERGENiHBG014652.
KOiK04440.

Enzyme and pathway databases

BRENDAi2.7.11.24. 6725.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01772. JNKMAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "JNK functions in the non-canonical Wnt pathway to regulate convergent extension movements in vertebrates."
    Yamanaka H., Moriguchi T., Masuyama N., Kusakabe M., Hanafusa H., Takada R., Takada S., Nishida E.
    EMBO Rep. 3:69-75(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, COFACTOR, TISSUE SPECIFICITY, ENZYME REGULATION.
    Tissue: Oocyte.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiMK08_XENLA
AccessioniPrimary (citable) accession number: Q8QHK8
Secondary accession number(s): Q8JJC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: April 1, 2015
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.