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Q8QGV6

- NLK2_XENLA

UniProt

Q8QGV6 - NLK2_XENLA

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Protein
Serine/threonine-protein kinase NLK2
Gene
nlk.2, nlk
Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Negatively regulates Wnt/beta-catenin-signaling during development. Plays a role together with sox11 in neural induction during early embryogenesis. Involved in TGFbeta-mediated mesoderm induction in early embryos, acting downstream of map3k7/tak1 to phosphorylate stat3.1. Augments the rnf138/narf-directed ubiquitination and degradation of tcf/lef by enhancing the association of rnf138/narf and tcf/lef. Phosphorylates mef2a to play a role in anterior neural development, including eye formation.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Magnesium By similarity.By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891ATP InferredBy similarity
Active sitei186 – 1861Proton acceptor By similarityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 749ATP By similarityBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. MAP kinase activity Source: UniProtKB-EC
  3. magnesium ion binding Source: UniProtKB
  4. protein binding Source: UniProtKB
  5. protein serine/threonine kinase activity Source: UniProtKB
  6. transcription factor binding Source: UniProtKB
  7. ubiquitin protein ligase binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. Wnt signaling pathway Source: UniProtKB-KW
  2. anterior/posterior pattern specification Source: UniProtKB
  3. mesoderm formation Source: UniProtKB
  4. nervous system development Source: UniProtKB
  5. peptidyl-serine phosphorylation Source: UniProtKB
  6. peptidyl-threonine phosphorylation Source: UniProtKB
  7. positive regulation of protein ubiquitination Source: UniProtKB
  8. regulation of transcription, DNA-templated Source: UniProtKB-KW
  9. serine phosphorylation of STAT3 protein Source: UniProtKB
  10. transcription, DNA-templated Source: UniProtKB-KW
  11. transforming growth factor beta receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway, Wnt signaling pathway

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase NLK2 (EC:2.7.11.24)
Alternative name(s):
Nemo-like kinase 2
Short name:
Nlk.2
Short name:
xNLK
Gene namesi
Name:nlk.2
Synonyms:nlk
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-1218927. nlk.2.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi89 – 891K → R: Kinase inactive. Does not enhance rnf138/narf-mediated ubiquitination or association of rnf138/narf and tcf/lef. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447Serine/threonine-protein kinase NLK2
PRO_0000370235Add
BLAST

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Expressed widely in the ectoderm during early gastrula stage when neural induction is taking place. Expressed in the head region of neurula stage embryos. At the end of neurulation, expression becomes localized to the nervous system, and is restricted to the central nervous system, eye and head neural crest cells by the early tadpole stages.2 Publications

Developmental stagei

Expressed maternally and throughout embryonic development through to the tadpole stage.1 Publication

Interactioni

Subunit structurei

Interacts with sox11, hmgxb4/hmg2l1, rnf138/narf, stat3.1 and mef2a.5 Publications

Protein-protein interaction databases

BioGridi99628. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ8QGV6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 349290Protein kinase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

KOiK04468.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8QGV6-1 [UniParc]FASTAAdd to Basket

« Hide

MAFPGSGRSI PGQLCAGVFS GLIQPPLGQK FYCPNGGGAV PSPLPQALSA    50
PQCNGEGRDP EPDRPIGYGA FGVVWSVTDP RDGKRVALKK MPNVFQNLVS 100
CKRVFRELKM LCFFKHDNVL SALDILQPPQ IDCFEEIYVI TELMQTDLHK 150
VIVSPQPLSS DHIKVFLYQI LRGLKYLHSA GILHRDIKPG NLLVNSNCVL 200
KICDFGLARV EELDESQHMT QEVVTQYYRA PEILMGSRHY RSAIDIWSVG 250
CIFAELLGRR ILFQAQSPIQ QLDLITDLLG TPPLTAMRSA CEGARAHILR 300
GPHKPPSLSV LYMLSGEATH EAVHLLCRML LFDPLKRISA KDALAHPYLE 350
EGRLRYHTCM CHCCYSVSSG RVYTADFEPT ATNRFDDSYE KSLTSVWQVK 400
ELVHRFITDQ HQGKRPPLCI NPHSAAFKTF IRSTAWHSSK VSKKEER 447
Length:447
Mass (Da):50,138
Last modified:June 1, 2002 - v1
Checksum:iF05DE96445D0FAE9
GO

Sequence cautioni

The sequence AAH77759.1 differs from that shown. Reason: Frameshift at position 128.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201S → G in AAH77759. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB071285 mRNA. Translation: BAB85870.1.
BC077759 mRNA. Translation: AAH77759.1. Frameshift.
RefSeqiNP_001082214.1. NM_001088745.1.
UniGeneiXl.7148.

Genome annotation databases

GeneIDi398295.
KEGGixla:398295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB071285 mRNA. Translation: BAB85870.1 .
BC077759 mRNA. Translation: AAH77759.1 . Frameshift.
RefSeqi NP_001082214.1. NM_001088745.1.
UniGenei Xl.7148.

3D structure databases

ProteinModelPortali Q8QGV6.
ModBasei Search...

Protein-protein interaction databases

BioGridi 99628. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 398295.
KEGGi xla:398295.

Organism-specific databases

CTDi 398295.
Xenbasei XB-GENE-1218927. nlk.2.

Phylogenomic databases

KOi K04468.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Involvement of NLK and Sox11 in neural induction in Xenopus development."
    Hyodo-Miura J., Urushiyama S., Nagai S., Nishita M., Ueno N., Shibuya H.
    Genes Cells 7:487-496(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH SOX11, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Oocyte.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.
  3. "Negative regulation of Wnt signalling by HMG2L1, a novel NLK-binding protein."
    Yamada M., Ohkawara B., Ichimura N., Hyodo-Miura J., Urushiyama S., Shirakabe K., Shibuya H.
    Genes Cells 8:677-684(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HMGXB4.
  4. "Role of the TAK1-NLK-STAT3 pathway in TGF-beta-mediated mesoderm induction."
    Ohkawara B., Shirakabe K., Hyodo-Miura J., Matsuo R., Ueno N., Matsumoto K., Shibuya H.
    Genes Dev. 18:381-386(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH STAT3.1.
  5. "NARF, an nemo-like kinase (NLK)-associated ring finger protein regulates the ubiquitylation and degradation of T cell factor/lymphoid enhancer factor (TCF/LEF)."
    Yamada M., Ohnishi J., Ohkawara B., Iemura S., Satoh K., Hyodo-Miura J., Kawachi K., Natsume T., Shibuya H.
    J. Biol. Chem. 281:20749-20760(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RNF138, MUTAGENESIS OF LYS-89.
  6. "Nemo-like kinase-myocyte enhancer factor 2A signaling regulates anterior formation in Xenopus development."
    Satoh K., Ohnishi J., Sato A., Takeyama M., Iemura S., Natsume T., Shibuya H.
    Mol. Cell. Biol. 27:7623-7630(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH MEF2A, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNLK2_XENLA
AccessioniPrimary (citable) accession number: Q8QGV6
Secondary accession number(s): Q6DD67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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